Simulated freeze-thaw cycles restructure plastisphere microbiomes and functional gene co-occurrence networks in alpine lake sediment microcosms.

Effects of Alhagi sparsifolia root content and soil moisture content on soil deformation and strength under different freeze-thaw temperature conditions

Unraveling the multi-scale mechanism of egg components in improving the freeze-thaw stabilization of frozen raw noodles.

Effects of milling methods on the properties of frozen corn dough: Insights into starch structural and physicochemical properties under repeated freeze-thaw cycles.

Thermo-hydro-mechanical (THM) coupling interactions in unsaturated soils with macropores under freeze-thaw cycles

New insight into concrete damage under the coupled action of low-stress dynamic loading and freeze-thaw cycles: Experiment and numerical simulation

Diclofenac sodium is a widely prescribed NSAID for inflammatory and rheumatoid diseases, and the dose adjustment, especially for elderly patients suffering from renal or hepatic impairment, is not a routine practice. In this study, a sensitive RP-HPLC bioanalytical method was developed and validated for quantifying diclofenac in human plasma. After quality assessment of different diclofenac sodium brands, a pharmacokinetic study was performed in 12 healthy human subjects and compared with predicted in silico PBPK models in healthy subjects and in patients with renal or hepatic impairment. The bioanalytical method demonstrated sensitivity and linearity from 20 to 3000 ng/mL, with 99.9% accuracy. Stability tests on plasma samples stored long term and subjected to freeze-thaw cycles showed considerable stability at -20°C. Pharmacokinetic parameters included Cmax, Tmax, AUC0-t and AUC0-∞. In silico PBPK modelling indicated that routine monitoring might not be necessary for patients with mild to moderate hepatic or renal impairment. However, significantly higher AUC0-t values were observed in severe cases, end-stage renal disease, cirrhosis B and cirrhosis C, suggesting a need for dose adjustments to mitigate dose-dependent toxicities. This study provides a comprehensive framework for generic manufacturers, regulatory agencies and clinicians for dose optimization, safety prediction and clinical decision support.

Modulations of shallow sea wave-related seismic noise revealed during a sea-ice freeze-thaw cycle.

From stability to application: mechanistic insights into the stability and 3d printing of probiotic-loaded pea protein-based high internal phase pickering emulsions.

Development of an ex vivo model for predicting in vivo tissue back pressure for subcutaneous injections.

ABSTRACT Accurate prediction of the freeze-thaw resistance of fiber-reinforced concrete (FRC) is crucial for optimizing its mix design and evaluating long-term durability. To enhance the accuracy of predicting FRC durability under freeze-thaw cycles, a dataset comprising 1,969 sets of FRC durability data was constructed. A machine learning hyperparameter optimization method based on the improved golden jackal optimization algorithm (PEGJO) was proposed for predicting FRC freeze resistance. The K-means++ algorithm was applied to cluster the data, balancing the training and testing sets. The PEGJO algorithm performs hyperparameter optimization for five machine learning algorithms: Support Vector Regression (SVR), Random Forest (RF), Kernel Ridge Regression (KRR), CatBoost, and XGBoost. Results indicate that the PEGJO-optimized XGBoost model achieved the highest predictive accuracy, with test set R2 values of 0.9581, 0.9585, and 0.9699 for compressive strength, relative dynamic modulus, and mass loss rate, respectively. Additionally, key characteristics influencing the durability of FRC were investigated using interpretable methodologies. To enhance practical applicability, an interactive graphical user interface based on the Tkinter framework was developed. This provides valuable guidance for FRC mix design, thereby advancing the field of performance evaluation for FRC.

Carbonation resistance and freeze thaw cycle performance of composite limestone powder tailings mixed sand concrete

Mitigating the Degradation of Microwave Self-Healing Efficiency in Asphalt Mixtures under Water Damage and Freeze–Thaw Cycles via Silicon Carbide Aggregates

Experimental study on the bonding strength of rebars in seawater sea-sand concrete under chloride erosion and freeze–thaw cycles

Freeze-thaw cycle and strain rate effect on energy dissipation mechanism and synergistic deterioration characterization of LFMOC

Hydroxytyrosol-based natural deep eutectic solvents (NADESs): Preparation, Cryoprotective mechanisms and their protective effects on the quality of freeze-thawed minced beef.

Sperm cryopreservation is a fundamental technique in assisted reproductive technologies. While human sperm freezing is clinically routine, preserving nonhuman primate sperm remains challenging due to species-specific characteristics and low post-thaw viability. This study aims to investigate the proteomic changes in rhesus macaque sperm before and after cryopreservation, focusing on identifying the molecular mechanisms behind cryoinjury. Semen was collected from six sexually mature male rhesus macaques (6-10 years) using rectal electroejaculation. Motility parameters were quantified pre- and postcryopreservation using computer-assisted semen analysis (CASA) with vapor-phase liquid nitrogen protocols. Data-independent acquisition (DIA) quantitative proteomics, combined with LC-MS/MS, was used to profile global protein expression changes. Differential protein analysis was performed using multibioinformatics tools for functional annotation and network modeling. Papanicolaou and acridine orange (AO) staining were used to assess sperm morphology and DNA integrity, while reactive oxygen species (ROS) assay and immunofluorescence were employed to validate the proteomic analysis results. We identified 512 differentially expressed proteins (32 upregulated, 480 downregulated), which are predominantly enriched in pathways related to metabolic reprogramming, oxidative stress response, and protein homeostasis. Immunofluorescence staining further confirmed markedly reduced levels of SOD1, PGK2, PKC‑alpha, and phospho‑tyrosine in sperm after freeze-thaw cycling. These molecular hubs are associated with sperm motility and functional stability and may be essential for regulating energy metabolism, oxidative balance, and proteotoxicity during cryopreservation-induced stress in rhesus macaque sperm. Cryopreservation induces profound proteomic remodeling in rhesus macaque sperm, mitochondrial electron transport, and metabolic homeostasis. Our findings reveal conserved and species-specific cryoinjury mechanisms, offering molecular targets for optimizing cryopreservation protocols in nonhuman primates to advance reproductive conservation strategies.

Study on material degradation mechanism of brick masonry defensive wall heritages under different environmental erosion

Click-crosslinked nanogels integrated into 3D stem cell spheroids enhance regenerative function for swallowing muscle repair.

Prediction of progressive sandstone fracturing under freeze-thaw cycles and seepage-stress coupling via cepstrum features and deep learning network optimization