Free Magnesium Research Articles

Microbiome profile and nutritional benefits of traditional overnight soaked cooked rice

This study explores the microbial composition, nutrient content, and health benefits of "Panta Bhat," a traditional dish made from cooked rice soaked overnight, commonly consumed in Southeast Asia, particularly in Bangladesh and India. The research aims to evaluate its potential as an affordable food to enhance nutrition and combat malnutrition in population where rice is a staple. The findings reveal that soaking cooked rice overnight initiates mild fermentation, leading to the consistent growth of beneficial bacteria from the Bacillota phylum. 16S rRNA sequencing identified two Gram-positive bacteria, Leuconostoc lactis and Weissella confusa, known for their probiotic and lactic acid-producing capabilities. Furthermore, mineral analysis via ICP-MS showed significant increases in free iron, zinc, manganese, boron, calcium, magnesium, and potassium in soaked rice compared to unsoaked rice. Notably, there was a significant reduction in glycaemic response after consuming soaked rice (P < 0.01). This study suggests that overnight-soaked cooked rice is a promising source of probiotics and enhanced micronutrients, making it a valuable and cost-effective, low-glycaemic food option.

Study on Expansion Rate of Steel Slag Cement-Stabilized Macadam Based on BP Neural Network.

The physicochemical properties of steel slag were investigated using SEM and IR, and it was found that free calcium oxide and free magnesium oxide in steel slag produce calcium hydroxide when in contact with water, leading to volume expansion. Thus, the expansion rate of steel slag itself was first investigated, and it was found that the volume expansion of steel slag was more obvious in seven days after water immersion. Then, the cement dosages of 5% and 6% of the steel slag expansion rate and cement-stabilized gravel volume changes between the intrinsic link were further explored after the study found that the cement bonding effect can be partially inhibited due to the volume of expansion caused by the steel slag, so it can be seen that increasing the dosage of cement can reduce the volume expansion of steel slag cement-stabilized gravel with the same dosage of steel slag. Finally, a prediction model of the expansion rate of steel slag cement-stabilized gravel based on the BP (back propagation) neural network was established, which was verified to be a reliable basis for predicting the expansion rate of steel slag cement-stabilized aggregates and improving the accuracy of the proportioning design.

Understanding the Thermodynamics of Magnesium Binding to RNA Structural Motifs.

Divalent magnesium ions (Mg2+) serve a vital role in defining the structural and catalytic chemistry of a wide array of RNA molecules. The body of structural information on RNA motifs continues to expand and, in turn, the functional importance of Mg2+ is revealed. A combination of prior work on the structural characterization of magnesium binding ligands with inner- and outer-sphere coordination modes, with recorded experimental binding energies for inner- and outer-sphere contacts, demonstrates the relative affinity and thermodynamic hierarchy for these sites. In turn, these can be correlated with cellular concentrations of free available magnesium ions, allowing the prioritization of populating important functional sites and a correlation with physiological function. This paper summarizes some of the key results of that analysis and provides predictive rules for the affinity and role of newly identified Mg binding sites on complex RNA structures. The influence of crystal packing on magnesium binding to RNA motifs, relative to their solution form, is addressed and caveats made.

Verification of the analytical performance of the serum magnesium assay on Abbott Architect ci8200

Magnesium is recognized as the fourth most important cation in the body and the second most abundant intracellular cation, following potassium. Its physiological significance lies in its capacity to form complexes with crucial intracellular anionic ligands, notably ATP, and its ability to compete with calcium for specific binding sites on proteins and cell membranes. Over 300 enzyme reactions rely on magnesium. The concentration of free magnesium within cells determines the magnesium content of these enzymes. Magnesium influences myocardial contraction and the electrical activity of myocardial cells, as well as the specialized conduction systems of the fetus, by modulating the transmembrane movements of ions like sodium, potassium, and calcium. Furthermore, magnesium can affect the contractility of vascular smooth muscle, and alterations in intracellular magnesium levels may impact cell proliferation or contraction. Magnesium is involved in nucleic acid and protein metabolism, intermediary metabolism, energy production, and consumption reactions, and it plays specific roles in various physiological systems such as the neuromuscular and cardiovascular systems. Our research aims to assess the analytical accuracy of the serum magnesium assay method utilizing an Abbott kit on the Architect ci8200 automated system in the biochemistry laboratory of Mohammed VI University Hospital of Oujda. The evaluation of the kit's analytical accuracy was conducted within the flexible scope A by conducting a performance analysis on the Architect ci8200. We examined repeatability, reproducibility, measurement imprecision, and compared results between two Architect ci8200 instruments. All findings align with the acceptability criteria recommended by the supplier and the Valtec protocol of the Société Française de Biologie Clinique (SFBC), indicating overall satisfactory outcomes for the study. The Architect ci8200 automated system demonstrated satisfactory analytical performance for reliably determining serum magnesium levels. Method verification in medical laboratories is essential to guarantee the accuracy, precision, and reliability of test results. Verification entails confirming that the test method used is suitable for its intended purpose, yields result consistent with the claimed performance characteristics, and meets the laboratory's quality control and quality assurance standards.

Impact of Incorporating Free Calcium and Magnesium on the Heat Stability of a Dairy- and Soy-Protein-Containing Model Emulsion.

This study investigated the impact of calcium chloride (CaCl2) and magnesium chloride (MgCl2) at varying concentrations on a model milk formulation's physical and chemical properties after thermal treatment. The model milk was subjected to two-stage homogenization and pasteurization before being supplemented with different concentrations of CaCl2 or MgCl2. The findings revealed that elevating the concentration of either calcium or magnesium resulted in the milk emulsion having a higher viscosity and median particle size following heating. CaCl2 had a slightly stronger impact than MgCl2, particularly at higher concentrations. The milk samples also exhibited a reduction in the zeta potential as the ionic strength of the salt solution increased, with the CaCl2-fortified milk displaying a slightly lower negative surface charge than the MgCl2-fortified milk at the same dose. The model milk's viscosity was evaluated after adding various salt concentrations and a temperature ramp from 20 to 80 °C. Notably, the viscosity and particle size changes demonstrated a non-linear relationship with increasing mineral levels, where a significant increase was observed at or above 5.0 mM. An emulsion stability analysis also revealed that the de-stabilization pattern of the high salt concentration sample differed significantly from its low salt concentration counterparts. These findings could serve as a basis for the future development of fortified UHT milk with nutritionally beneficial calcium and magnesium in industrial applications.

Enhanced strategies for phosphate recovery from urine by magnesium galvanic process

Magnesium galvanic process (MGP) can be applied to recover phosphate from source-separated urine. However, information on how the urine matrix affects MGP performance is limited. Therefore, this study investigated the mechanism of phosphate recovery by MGP in synthetic and real urine matrixes. Our results showed that the major components in urine (i.e., NH4+, Cl−, and HCO3−) all exhibited acceleration effects on corrosion of Mg plate. However, the underlying action mechanism of each component was distinct. Ammonium facilitated the conversion from MgO to Mg(OH)2, chloride complexed with Mg2+ ions, and bicarbonate led to complexation as well as formation of MgCO3. Furthermore, our results revealed an interesting aspect where although bicarbonate alone accelerated the corrosion of Mg plate, its coexistence with other ions inhibited overall performance due to the blocking effect of formed MgCO3 on chloride penetration and reduction in free magnesium ion concentration. After elucidating the interaction of NH4+, Cl−, and HCO3− on the passive layer of the Mg plate, we proposed to pretreat urine with HCl, which resulted in a significant enhancement in current production and phosphate recovery. This improved MGP was further tested in a continuous flow reactor, which recovered over 95% of phosphate in real urine for more than 1 h. The phosphate precipitates were confirmed as high purity struvite. Generally, the improved MGP, which simultaneously produced Mg2+, dihydrogen, and electricity with no energy input, is a promising sustainable and green alternative for phosphate recovery from source-separated urine.

Loss of CorA, the primary magnesium transporter of Salmonella, is alleviated by MgtA and PhoP-dependent compensatory mechanisms.

In many Gram-negative bacteria, the stress sigma factor of RNA polymerase, σS/RpoS, remodels global gene expression to reshape the physiology of stationary phase cells and ensure their survival under non-optimal growth conditions. In the foodborne pathogen Salmonella enterica serovar Typhimurium, σS is also required for biofilm formation and virulence. We have recently shown that a ΔrpoS mutation decreases the magnesium content and expression level of the housekeeping Mg2+-transporter CorA in stationary phase Salmonella. The other two Mg2+-transporters of Salmonella are encoded by the PhoP-activated mgtA and mgtB genes and are expressed under magnesium starvation. The σS control of corA prompted us to evaluate the impact of CorA in stationary phase Salmonella cells, by using global and analytical proteomic analyses and physiological assays. The ΔcorA mutation conferred a competitive disadvantage to exit from stationary phase, and slightly impaired motility, but had no effect on total and free cellular magnesium contents. In contrast to the wild-type strain, the ΔcorA mutant produced MgtA, but not MgtB, in the presence of high extracellular magnesium concentration. Under these conditions, MgtA production in the ΔcorA mutant did not require PhoP. Consistently, a ΔmgtA, but not a ΔphoP, mutation slightly reduced the magnesium content of the ΔcorA mutant. Synthetic phenotypes were observed when the ΔphoP and ΔcorA mutations were combined, including a strong reduction in growth and motility, independently of the extracellular magnesium concentration. The abundance of several proteins involved in flagella formation, chemotaxis and secretion was lowered by the ΔcorA and ΔphoP mutations in combination, but not alone. These findings unravel the importance of PhoP-dependent functions in the absence of CorA when magnesium is sufficient. Altogether, our data pinpoint a regulatory network, where the absence of CorA is sensed by the cell and compensated by MgtA and PhoP- dependent mechanisms.

Evaluation of Potential Factors Affecting Steel Slag Carbonation

Steel slag is a solid waste product generated during the carbonation stage of steelmaking. It has high levels of heavy metals and substantial amounts of free calcium and magnesium oxide, making it unsuitable for use as a cement material. Furthermore, the disposal of steel slag in landfills requires many resources and can seriously contaminate the surrounding environment. One method of reducing its negative environmental impact is carbonation, which involves reacting steel slag with carbon dioxide to form stable minerals. However, many parameters influence the carbonation efficiency of steelmaking slag, including temperature, time, particle size, pressure, CO2 concentration, liquid-to-solid ratio, moisture content, humidity, additives, etc. To this end, this paper comprehensively reviews the most important steel slag carbonation-influencing factors. Moreover, it compares the characteristics from two perspectives based on their causes and effects on carbonation. Finally, this article reviews earlier studies to identify the factors that affect steel slag carbonation and the potential of carbonated steel slag as a sustainable construction material. Based on previous research, it systematically examines all the elements for future work that need to be improved.

Use of Basic Oxygen Furnace Slag (BOFS) in Superpave Mix Design: BOFS Aggregate Properties

This research aims to evaluate the properties of basic oxygen furnace slag (BOFS) as aggregates used in Superior Performing Asphalt Pavements (Superpave) mix design. A total of five different aggregates, including one limestone and four BOFS aggregates with different ages, were evaluated by the following aggregate properties: specific gravity, water absorption, Los Angles abrasion, aggregate crushing value, angularity, flakiness, soundness (sulfate chemical and freeze-thaw resistance), and expansion characteristics. Then, the volume stability of the hot mix asphalt (HMA) mixture was also discussed based on the aging effect of BOFS aggregate. Test results indicated that aged BOFS aggregate performs better than unaged BOFS aggregate: Aged BOFS aggregate has lower water absorption, lower aggregate crushing value, higher abrasion resistance, and less expansion. The flakiness and angularity of BOFS aggregates were satisfied with Superpave criteria. However, if free-lime (f-CaO) and free magnesium (f-MgO) in BOFS aggregate exist, it may cause the volume stability of asphalt mixtures with BOFS aggregate. In conclusion, if BOFS is appropriately aged and weathered, BOFS can be an alternative to conventional aggregate.

Improving diagnosis and treatment of hypomagnesemia.

Magnesium is one of the most abundant cations in the body and acts as a cofactor in more than 600 biochemical reactions. Hypomagnesemia is a highly prevalent condition, especially in subjects with comorbid conditions, but has received less attention than other electrolyte disturbances. This review will discuss magnesium physiology, absorption, storage, distribution across the body, and kidney excretion. After reviewing the regulation of magnesium homeostasis, we will focus on the etiology and clinical presentation of hypomagnesemia. The role of laboratory medicine in hypomagnesemia will be the main purpose of this review, and we will discuss the laboratory tests and different samples and methods for its measurement. Although free magnesium is physiologically active, total serum magnesium is the most commonly used measurement in laboratory medicine and is apt for clinical purposes; however, it is not appropriately used, and many patients with hypomagnesemia remain undiagnosed and not treated. Using information technologies, laboratory medicine can largely improve the diagnosis and treatment of hypomagnesemia through the design and establishment of automatic demand management and result management interventions by acting in the first and last steps of the laboratory cycle, test requests, and actions taken after test results, to unmask patients with hypomagnesemia and improve the number of patients undergoing treatment.

Hydration characteristics of tailing mud–42.5 OPC composite cementitious system and its application to dry-mixed mortar

This study investigated the influence of the raw material ratio on the product quality of tailing mud with type-I 42.5 ordinary Portland cement (OPC) in a binary cementitious system. In particular, this research aimed to expand the applicability of tailing mud and tailing waste rock while minimizing the production cost of dry-mixed mortar. The results demonstrated a synergistic reaction between the tailing mud and 42.5 OPC, which was beneficial for enhancing hydration and gelation, increasing the production of calcium silicate hydrate (C–S–H) and calcium hydroxide (C–H), promoting more free magnesium oxide (MgO) in the cement to participate in the hydration reaction, and improving the structure of the composite gelation system. When the concentration of tailing mud in 42.5 OPC was 20%, the cementitious block attained a 28-day compressive strength of 44.85 MPa. To manufacture the dry-mixed mortar product, 80% mud and 20% 42.5 OPC were mixed with equal amounts of tailing mud and waste rock aggregate. For the dry-mixed mortar, the 28-day compressive strength was 7.32 MPa, 2-h consistency-loss rate was 12.4%, and water retention rate was 99.2%. To further explore the synergistic reaction mechanism between the tailing mud and 42.5 OPC, the energy calculations, geometric optimization, and dynamic modeling of the tailing mud–42.5 OPC cementitious system were performed using the Forcite module in Materials Studio software. Overall, the results indicated that including tailing mud improved the mechanical strength of 42.5 OPC.

Effect of addition of intrathecal preservative free magnesium sulfate with 0.5% bupivacaine heavy and fentanyl with 0.5% bupivacaine heavy on post-operative pain relief in patients undergoing hysterectomy

Background: Spinal anesthesia is the most common neuraxial anesthesia for infraumblical surgeries. The use of various adjuvants has become popular in today’s scenario to enhance its quality. Aims and Objectives: The aim of this study was to evaluate the onset, duration of sensory and motor block, hemodynamic effects (if any), duration of post-operative analgesia, and adverse effects of fentanyl or magnesium given intrathecally with 0.5% bupivacaine heavy in patients undergoing hysterectomy. Materials and Methods: This prospective randomized double-blinded study was conducted in total sixty patients undergoing hysterectomy, divided into two groups of 30 patients each. Group F received 25 μg fentanyl with 3 mL 0.5% bupivacaine heavy and Group M received 100 mg magnesium sulfate with 3 mL 0.5% bupivacaine heavy. The onset and duration of sensory and motor blockade, duration of analgesia, hemodyamics, and side effects were assessed. Results: The mean time of onset of sensory and motor block was less in fentanyl (P&lt;0.001). Duration of sensory, motor block, and duration of analgesia was more in fentanyl group (P&lt;0.001), whereas incidence of side effects such as bradycardia, hypotension, and shivering was less in magnesium. Conclusion: Our study concluded that addition of fentanyl as adjuvant effectively augmented the quality of spinal anesthesia, but magnesium provided stable hemodynamics and lesser side effects as compared to fentanyl.

Study on vibrational Raman modes and electric properties of lead-free sodium bismuth titanate – bismuth magnesium zirconate piezoelectric ceramics

Raman spectra of lead- free piezoelectric sodium bismuth titanate-bismuth magnesium zirconate (1-x)(Na0.5Bi0.5)TiO3-xBi(Mg0.5Zr0.5)O3 [NBT-BMZ] ceramics are recorded and analyzed. Vibration phonon frequencies associated to Bi-O, Na-O, TiO6 octahedra and oxygen vibrations are noticed. Intensity changes on E(TO1) mode at 110 cm−1 arising from Bi-O vibrations is due to difference in polarizability of substituted cations in the A-site of NBT. Frequency shift in 247 and 281 cm−1 mode are attributed to lattice change and TiO6 octahedral rotation induced by substitution of BMZ in NBT. Increase of E(TO4) and A1(TO4) vibration modes due to decrease of TiO6 octahedral rotation reduces the lattice distortion and homogeneous strain which in turn prompts softening of coercive field and enhances remnant polarization as well as dielectric constant in NBT-BMZ piezoceramics. Also, vibrational Raman modes E(TO1), E(TO4) and A1(TO4) are the decisive phonons for interpreting the interesting dielectric behavior of NBT-based ceramics.

Comparison of Effect of Preservative Free Ketamine and Magnesium Sulphate used as an Adjuvant to 0.5 % Hyperbaric Bupivacaine in Orthopaedic Lower Limb Surgeries under Spinal Anaesthesia - A Prospective Double Blind Randomised Control Study

Comparison of Effect of Preservative Free Ketamine and Magnesium Sulphate used as an Adjuvant to 0.5 % Hyperbaric Bupivacaine in Orthopaedic Lower Limb Surgeries under Spinal Anaesthesia - A Prospective Double Blind Randomised Control Study

Assessing the Performance of a Sewage Treatment Plant in Habbak, Srinagar: A Physicochemical Analysis

A study was carried out to assess the physicochemical profile of one of the sewage treatment plants in the Habak region of Srinagar city. The water samples from the inlet and outlet of the plant were analyzed for various limnological parameters like temperature, dissolved oxygen, free carbon dioxide, alkalinity, calcium and magnesium hardness, orthophosphate etc. The average water temperature recorded at the outlet and inlet ranged between 16.56 to 18.03oC, free carbon dioxide between 50.06 to 78.03 mg/l, alkalinity between 328 to 389 mg/l, calcium hardness between 83.41 to 85.12 mg/l, magnesium hardness between 614.58 to 783.53 mg/l, orthophosphate between 503.56 to 650μg/l respectively. The dissolved oxygen content at the inlet was found to be zero during all times in the study while the average value at the outlet was found to be 5.2 mg/l. All the parameters were found to be non-significant except for water temperature, dissolved oxygen, and total hardness.

Exceptional reversed yield strength asymmetry in a rare-earth free Mg alloy containing quasicrystal precipitates

This work reports an exceptional reversed yield strength asymmetry at room temperature for a rare-earth free magnesium alloy containing a mass of fine dispersed quasicrystal (I-phase) precipitates. Although exhibiting traditional basal texture, it owns an exceptional CYS/TYS as high as ∼1.17. Electron back-scattered diffraction (EBSD) and transmission electron microscopy (TEM) examinations indicate pyramidal <c + a> and prismatic <c> dislocations plus tensile twinning being activated after immediate yielding in compression while basal and non-basal <a> dislocations in tension. I-phase particles transferred the concentrated stress by self-twinning to provide the driving force for tensile twin initiating in neighboring grains, thereby significantly increasing the critical resolved shear stress of tensile twinning to possibly the level of pyramidal <c + a> slip, finally leading to the dominance of pyramidal <c + a> slip plus tensile twinning in texture grains. This results in a higher contribution on yield strength by ∼55 MPa in compression than in tension, which reasonably agrees with the experimental yield strength difference (∼38 MPa). It can be concluded that I-phase particles influence deformation modes in tension and in compression, finally result in reversed yield strength asymmetry.

Effects of Magnesium, Pyrophosphate and Phosphonates on Pyrophosphorolytic Reaction of UDP-Glucose Pyrophosphorylase.

UDP-glucose pyrophosphorylase (UGPase) carries a freely reversible reaction, using glucose-1-P and UTP to produce UDP-glucose (UDPG) and pyrophosphate (PPi), with UDPG being essential for glycosylation reactions in all organisms including, e.g., synthesis of sucrose, cellulose and glycoproteins. In the present study, we found that free magnesium (Mg2+) had profound effects on the reverse reaction of purified barley UGPase, and was absolutely required for its activity, with an apparent Km of 0.13 mM. More detailed analyses with varied concentrations of MgPPi allowed us to conclude that it is the MgPPi complex which serves as true substrate for UGPase in its reverse reaction, with an apparent Km of 0.06 mM. Free PPi was an inhibitor in this reaction. Given the key role of PPi in the UGPase reaction, we have also tested possible effects of phosphonates, which are analogs of PPi and phosphate (Pi). Clodronate and etidronate (PPi analogs) had little or no effect on UGPase activity, whereas fosetyl-Al (Pi analog), a known fungicide, acted as effective near-competitive inhibitor versus PPi, with Ki of 0.15 mM. The data are discussed with respect to the role of magnesium in the UGPase reaction and elucidating the use of inhibitors in studies on cellular function of UGPase and related enzymes.

Nuclear Magnetic Resonance-Measured Ionized Magnesium Is Inversely Associated with Type 2 Diabetes in the Insulin Resistance Atherosclerosis Study.

The aims were to optimize a nuclear magnetic resonance (NMR)-based assay for quantifying ionized or free magnesium and investigate its association with type 2 diabetes (T2D). A high-throughput, ionized magnesium assay was optimized and evaluated. Plasma magnesium was quantified, and associations with T2D were ascertained in Insulin Resistance Atherosclerosis Study (IRAS) participants. Coefficients of variation for the ionized magnesium assay ranged from 0.7–1.5% for intra-assay and 4.2–4.7% for inter-assay precision. In IRAS (n = 1342), ionized magnesium was significantly lower in subjects with prediabetes and T2D than in normoglycemic subjects, and lower in participants with T2D than those with prediabetes (p < 0.0001). Cross-sectional regression analyses revealed that magnesium was associated with T2D at baseline in models adjusted for multiple clinical risk factors (p = 0.032). This association appeared to be modified by sex, in such a way that the associations were present in women (OR = 0.54 (95% CI 0.37–0.79), p = 0.0015) and not in men (OR = 0.98 (95% CI 0.71–1.35), p = 0.90). Longitudinal regression analyses revealed an inverse association between magnesium and future T2D in the total population (p = 0.035) that was attenuated by LP-IR (p = 0.22). No interactions were detected between magnesium and age, race, BMI, glucose, insulin, triglycerides, or LPIR for the prospective association with future T2D. However, a significant interaction between magnesium and sex was present, now with a trend for an association in men (OR = 0.75 (95% CI 0.55–1.02), p = 0.065 and absence of an association in women (OR = 1.01 (0.76–1.33), p = 0.97). Conclusions: lower ionized magnesium, as measured by an NMR-based assay optimized for accuracy and precision, was associated cross-sectionally with T2D at baseline and longitudinally with incident T2D in IRAS.

31 P MRSI at 7 T enables high-resolution volumetric mapping of the intracellular magnesium ion content in human lower leg muscles.

The non-invasive determination of the free magnesium ion concentration ([Mg2+free ]) using 31 P MRSI in vivo is of interest in research on various pathologies, e.g. diabetes. The purpose of this study was to demonstrate the potential of 31 P MRSI at 7 T to enable volumetric, high-resolution mapping of [Mg2+free ]. 3D 31 P MRSI datasets from the lower leg of three healthy volunteers were acquired at B0 = 7 T with a nominal spatial resolution of (8× 8× 16)mm3 in 56 min. Volumetric [Mg2+free ] maps were calculated based on the quantified local chemical shift difference between the α- and β-resonance of adenosine triphosphate (ATP) considering also local pH values. Mean [Mg2+free ] values from three different muscle groups were compared. To demonstrate the potential of reducing the measurement time, the analysis was repeated on the acquired MRSI data retrospectively reconstructed with fewer averages. The generated [Mg2+free ] maps revealed local differences, and mean [Mg2+free ] values of (1.08 ± 0.03)mM were found in the tibialis anterior, (0.91 ± 0.04)mM in the soleus and (0.98 ± 0.03)mM in the gastrocnemius medialis. The time-reduced 28-min scan resulted in comparable [Mg2+free ] maps, and mean values being in agreement with the values from the 56-min scan. 31 P MRSI at 7 T enables volumetric, high-resolution mapping of free magnesium ion content in human lower leg muscles. The measurement time of the 31 P MRSI acquisition can be reduced to 28 min, opening the potential to apply volumetric [Mg2+free ] mapping for the investigation of pathologies with altered magnesium homeostasis.

Studies on freshwater crab population as influencedby physico-chemical attributes in Gho-Manhasanstream, a tributary of river Chenab

Crustaceans are distributed world-wide and are found in a variety of habitats. Freshwater crabs constitute one-fifth of the brachyurans all over the world. Freshwater crabs are the eminent indicators of good water quality. The present study was carried out with an aim to assess the influence of various physico-chemical parameters such as temperature, pH, depth, dissolved oxygen, free carbon dioxide, calcium, magnesium, bicarbonates and chlorides on the population of crabs in the Gho-Manhasan stream, a tributary of river Chenab. A comprehensive study of these parameters and a comparative analysis with those recorded in last eight years span gave a clear picture of the pollution stress due to anthropogenic activities in the water body that has led to a considerable decline in the population of crabs in this stream. This emphasizes the need to spotlight and improve measures of conservation before there is decline in the crab population to the levels from which they cannot recuperate.