Soybean Fractions Research Articles

Unravelling the environmental drivers determining the residual fraction of soybean seed

Unravelling the environmental drivers determining the residual fraction of soybean seed

Innovative Fusion-Based Strategy for Crop Residue Modeling

The purpose of this study was to present a new strategy based on fusion at the decision level for modeling the crop residue. To this end, a set of satellite imagery and field data, including the Residue Cover Fraction (RCF) of corn, wheat and soybean was used. Firstly, the efficiency of Random Forest Regression (RFR), Support Vector Regression (SVR), Artificial Neural Networks (ANN) and Partial-Least-Squares Regression (PLSR) in RCF modeling was evaluated. Furthermore, to increase the accuracy of RCF modeling, different algorithms results were combined based on their modeling error, which is called the decision-based fusion strategy. The R2 (RMSE) between the actual and modeled RCF based on ANN, RFR, SVR and PLSR algorithms for corn were 0.83 (3.89), 0.86 (3.25), 0.76 (4.56) and 0.75 (4.81%), respectively. These values were 0.81 (4.86), 0.85 (4.22), 0.78 (5.45) and 0.74 (6.20%) for wheat and 0.81 (3.96), 0.83 (3.38), 0.76 (5.01) and 0.72 (5.65%) for soybean, respectively. The error of corn, wheat and soybean RCF estimating decision-based fusion strategy was reduced by 0.90, 0.96 and 0.99%, respectively. The results showed that by implementing the decision-based fusion strategy, the accuracy of the RCF modeling was significantly improved.

Isolation and characterization of antihypertensive peptides from soy bean protein

Proteins and peptides are the most diverse biomolecules found in nature and make our interest due to their wide applications in food and pharmaceutical industry. Angiotensin Converting Enzyme (ACE) plays a major role in controlling blood pressure. The inhibition of ACE with peptides is a main target in the regulation of hypertension. The objective of the present study was to investigate the therapeutic potential of soy bean. This was accomplished by isolation of ACE inhibitory peptides using response surface methodology (RSM) and characterization of these bioactive peptides by mass spectrometry. 31 hydrolyzed fractions were isolated and evaluated for their ACE inhibition potential. Hydrolyzed fraction having highest ACE inhibitory activity was characterized by liquid chromatography-mass spectrometry (LC-MS) technique. RSM results showed maximum ACE inhibition potential (64%) by hydrolyzate was obtained at 45 oC temperature, pH 8.0, E/S 0.2 in 2 hours hydrolysis time. Results of LC-MS analysis revealed Ser-Gly, Ser-Pro, Met-Ala, His-Ala, Lys-Pro, Phe-Thr, Met-Leu, Pro-Arg, Ala-Pro-Val, Pro-Ala-Leu, Val-Met-Gly, Pro-Leu-Val, Pro-Pro-Gln, His-Arg-Gly, Ser-Phe-Val-Leu, Ala-Val-His-Try, Arg-Thr-Val-Arg, His-His-Tyr-Leu-Val, Asp-Gly-Ala-Cys-Ser-Ala-Asn and MetVal-Thr-Gly-Pro-Gly-Cys-His bioactive peptides in hydrolyzed fraction of soy bean. Our data provide evidence that response surface methodology is a good approach for isolation of antihypertensive bioactive peptides with more potent activity as nutraceuticals or pharmaceuticals. Therefore soy bean can be use for industrial production of pharmaceutical grade natural medicines for handling high blood pressure.

PENAMBAHAN KULIT ARI BIJI KEDELAI HASIL FERMENTASI MENGGUNAKAN EM-4 DALAM FORMULASI RANSUM PELLET BROILER TERHADAP FRAKSI SERAT

Source of feed raw materials can be obtained from various wastes, one of which is industrial waste is soybean seed, processing soybean seed, which has the potential as broiler ration in the form of pellets. This study aims to evaluate the fraction of broiler pellet fiber fermented soybean seed (KABKF) using Effective Microorganism-4 with different storage times. The research method used was an experimental method with a completely randomized design consisting of 5 treatments and 4 replications. The variables in this study were the fiber fraction content which included NDF (%), ADF (%), ADL (%), hemicellulose (%) and cellulose (%). The results of this study indicate that the longer the storage of broiler pellet rations can reduce the NDF content (75.50% - 53.31%), ADF (57.57% - 41.56%), and ADL (12.33% - 5.73%). ) and had not been able to increase the content of hemicellulose (11.86% - 17.93%) and cellulose (42.39-29.78%. The conclusion of this study is that 8 weeks of storage has the best treatment in reducing the content of NDF%, ADF%, and ADL%

Genomic regions controlling seed weight, water absorption ratio, sprout yield, and storage protein fractions in soybean

ABSTRACT Physicochemical traits, such as 100-seed weight (100-SW), water absorption ratio (WAR), sprouting and storage protein fractions of soybean (Glycine max (L.) Merr.) seeds, contribute to the quality of soy products processed from them. In the present investigation, 108 recombinant inbred lines (RILs) derived from LSb1 × NRC7 were genotyped using 105 polymorphic simple sequence repeat (SSR) markers across the genome. These RILs were phenotyped for 100-SW, WAR, sprout yield, hypocotyl length, and glycinin (11S), and β-conglycinin (7S) subunits of storage protein also. The study revealed common genomic regions governing multiple seed traits, besides the identification of quantitative trait loci (QTL) for individual seed traits. Of the seven QTL identified for 100-SW and 10 for WAR, five QTL were novel for both the traits. All four QTL identified for sprout yield and two QTL for 11S/7S ratio were novel. Five QTL were identified for multiple seed traits. These genomic regions common to different seed traits should be useful for pyramiding desirable traits for 100-SW, WAR, sprout yield, and 11S/7S ratio in the same genetic background for developing soybean varieties suitable for processing food products.

Physicochemical and manufacturing cost elements of complementary food formulations from broken fraction of rice cultivars, soybean and sorghum malt

The broken fractions of rice cultivars improved for yield, grain quality, amylose content and tolerance to common production constraints consisting of FARO 44, FARO 52, NERICA L-34, NERICA L-19 and LOCAL RICE. The rice cultivars, Sorghum malt and soybean were processed. Evaluation of the physicochemical properties of these new African rice cultivars together with their products yield and utilization (value addition) were the main objective of this study. The experimental design for these infant food formulations (5x2x2 factorial design) consisted of those 5 rice cultivars, sorghum malt (0, 5%) and soybeans flour (0, 30%). These formulations together with the raw materials were evaluated for their physicochemical properties. Manufacturing cost of formulations with ratios of rice to soybeans 70:30 with malt were selected and quantified. Results of particle size distributions showed significant (p < 0.05) difference. However, 98.38 and 94.90% of raw materials and blends respectively, successfully passed through 600 µm sieve aperture. Mesh 300 and 180 µm were found to retain the highest percentage particles. Functional properties of raw materials and blends were within the recommended range. More importantly, pH values of blends were comparable to infants’ natural milk drinks. Proximate composition of raw materials and blends contain 4.14 to 9.59% moisture, 0.37 to 5.12% ash, 8.25 to 45.35% protein, 0.41 to 20.00% fat and 26.54 to 82.59% carbohydrate. Blends fiber and energy ranged from 1.27 to 4.33 and 362.95 to 388.71 Kcal (1541.92 to 1643.04 KJ) respectively. Formulations with ratios of rice to soybeans 70:30 with malt and those of rice 100% alone with malt were observed suitable as follow-on formulae and for infant(s) with critical protein related ailments, respectively. Net profit generated is more significant than interest rate that could have been obtained from bank saving system

Purification and Characterization of Antioxidant Peptides from Alcalase-Hydrolyzed Soybean ( Glycine max L.) Hydrolysate and Their Cytoprotective Effects in Human Intestinal Caco-2 Cells.

This study aimed to purify and identify antioxidant peptides from the low-molecular-weight fraction (SPH-I, MW < 3 kDa) of Alcalase-hydrolyzed soybean ( Glycine max L.) hydrolysate and further evaluate the cytoprotective effects of synthesized peptides against oxidative stress in human intestinal Caco-2 cells. After purification by gel filtration chromatography and reversed-phase HPLC, four major peptides were sequenced by nano-LC-ESI-MS/MS as VVFVDRL (847 Da, SPH-IA), VIYVVDLR (976 Da, SPH-IB), IYVVDLR (877 Da, SPH-IC), and IYVFVR (795 Da, SPH-ID). The antioxidant peptides were synthesized and displayed desirable DPPH radical-scavenging activity (from 16.5 ± 0.5 to 20.3 ± 1.0 μM Trolox equivalent (TE)/μM), ABTS•+ radical-scavenging activity (from 3.42 ± 0.2 to 4.24 ± 0.4 mM TE/μM), ORAC (from 143 ± 2.1 to 171 ± 4.8 μM TE/μM), and FRAP (from 54.7 ± 1.2 to 79.0 ± 0.6 mM Fe2+/μM). Moreover, the synthesized peptides protected Caco-2 cells against H2O2-induced oxidative damage via significantly downregulating intracellular ROS generation and lipid peroxidation ( p < 0.05). Additionally, SPH-IC and SPH-ID statistically upregulated total reduced glutathione synthesis, enhanced activities of catalase and glutathione reductase, and suppressed ROS-mediated inflammatory responses via inhibiting interleukin-8 secretion ( p < 0.05).

Germination changes the isoflavone profile and increases the antioxidant potential of soybean

Isoflavones, which exist in their conjugated or aglycone forms, are well recognized for their potential health benefits. However, isoflavones as aglycone have been regarded as the most bioactive form. In the present study, the profile of isoflavones and their scavenging activity as affected by germination were investigated in several soybean fractions, namely cotyledons, epicotyls, radicles, and hypocotyls. Only aglycones were detected in the radicles from 144 h until 168 h of germination, which makes this component a potential feedstock for studies aiming at isolation, especially of daidzein, which was present in higher concentrations. In terms of total yield and contribution to the total weight of the germinated soybeans, the cotyledons are the best source of aglycones, which was achieved at 144 h of germination. The higher scavenging activity of high-aglycone components from germinated soybeans supports the use of germination to obtain functional foods and/or ingredients with potentially superior bioactivities.

Aflatoxin B1 and zearalenone in soybeans: occurrence and distribution in whole and defective kernels

ABSTRACTFew studies have addressed the distribution of mycotoxins in soybean and/or their processing fractions. In this study, samples from commercial lots were collected in four Brazilian states. The distribution of mycotoxins in soybean fractions, according to their commercial grading system, namely whole kernels (WK), split, broken and crushed kernels (SBCK), damaged kernels (DK), heat damaged and burned kernels (HDBK), moldy kernels (MK), greenish kernels (GK), foreign material + impurities (FMI), were analyzed using HPLC-FLD. AFB1 and ZEN tested positive in 43.3 and 80%, respectively. The incidence of AFB1 was higher in MK (50%), followed by HDBK (30.4%) and FMI (26.0%). ZEA incidence ranged from 69% (SBCK) to 100% (HDBK). Co-occurrence (53.3%) in at least one fraction was also detected. Brazil is the second world producer of soybeans, which places the country in a very important position. Therefore, the information provided is crucial and timely relevant for the industry and policymakers.

Auxin-to-Gibberellin Ratio as a Signal for Light Intensity and Quality in Regulating Soybean Growth and Matter Partitioning.

The intensity and quality (red to far-red (R/Fr) ratio) of light directly affect growth of plant under shading. Gibberellins (GAs) and auxin [indole-3-acetic acid (IAA)] play important roles in mediating the shading adaptive responses of plants. Thus, the intensity and quality of the uncoupling light from shading were assessed to identify the influence of each component on the morphology and matter distribution of the leaf, stem, and petiole. This assessment was based on the changes in endogenous Gibberellin 1 (GA1) and IAA levels. Soybean plants were grown in a growth chamber with four treatments [normal (N), N+Fr, low (L), and L+Fr light]. Results revealed that the reductions in photosynthetically active radiation (PAR) and R/Fr ratio equally increased height and stem mass fractions (SMFs) of the soybean seedling. The light intensity significantly influenced the dry mass per unit area and mass fraction of soybean leaves, whereas the light quality regulated the petiole elongation and mass fraction. Low R/Fr ratio (high Fr light) increased the soybean biomass by improving the photosynthetic assimilation rate and quantum yield of photosystem II. In addition, the IAA and GA1 levels in the leaf, stem, and petiole did not reflect the growth response trends of each tissue toward light intensity and quality; however, trends of the IAA-to-GA1 content ratios were similar to those of the growth and matter allocation of each soybean tissue under different light environments. Therefore, the response of growth and matter allocation of soybean to light intensity and quality may be regulated by the IAA-to-GA1 content ratio in the tissues of the soybean plant.

Fractionation, physicochemical, and structural characterization of winged bean seed protein fractions with reference to soybean

ABSTRACTProteins from defatted flour winged bean seed (Psophocarpus tetragonolobus) were sequentially fractionated with reference to soybean seed (Glycine max) using distilled water, 25 g/kg sodium chloride solution, 0.1 M NaOH, and 70% alcohol and subjected to physicochemical, functional, and structural analysis. Winged bean seed had globulin (31.94%) and albumin (26.46%), with denaturation temperatures (Td) of 92.82 and 82.76°C, and surface hydrophobicities (SHs) of 214.51 and 189.32, respectively, whereas soybean seed had 34.95% globulin and 32.82% albumin, with respective Td of 81.68 and 90.63°C, and SH of 210.80 and 204.66. This indicates that winged bean albumin could perform better at water/oil interface in an oil-in-water emulsion system because of it higher hydrophobicity, although soybean albumin had higher extraction rate. Both legumes’ proteins showed comparable gelation when employed for their gelling ability to form three-dimensional network in food systems. Soybean proteins had significantly (P < 0.05) higher foaming capacities, but winged bean proteins foams were more stable and soluble over broad pH, good for manufacture of ice cream and protein beverages, respectively. Though β-sheets and β-turns were the major secondary structures of both protein fractions, soybean albumin had significantly (P < 0.05) higher β-sheets than winged bean albumin, whereas winged bean globulin and glutelin had higher content of β-sheets than corresponding soybean fractions. The low amounts of β-sheets in the winged bean albumin and soybean globulin could render them more digestible than the corresponding soybean and winged bean protein fractions.Abbreviations: WB: Winged bean; SB: soybean; Td: denaturation temperature; SH: surface hydrophobicity; SH: surface hydrophobicity; W-ALB: winged bean albumin; W-GLO: winged bean globulin; W-GLU: winged bean glutelin; W-PRO: winged bean prolamin; W-ISO: winged bean isolate; S-ALB: soy albumin; S-GLO: soy globulin; S-GLU: glutelin; S-ISO: soy glutelin and S-PRO: soy prolamin.

Comparison of alkaline and acid pretreatments for enzymatic hydrolysis of soybean hull and soybean straw to produce fermentable sugars

The specific characteristics of biomass structure and differences in chemical composition of soybean hull (SBH) and soybean straw (SBS) may result in different behavior of pretreatment and enzymatic hydrolysis. In this work, dilute sulfuric acid (DA) pretreatment and alkaline sodium hydroxide (AL) pretreatment in a range of pretreatment temperatures and durations were investigated to improve the enzymatic digestibility of SBH and SBS. Satisfactory enzymatic digestibility was observed in the hydrolysis of both pretreated soybean fractions, but SBH showed higher digestibility than that of SBS, no matter which pretreatment technology was applied. Furthermore, DA pretreatment was more effective than AL pretreatment in conversion of soybean fractions into fermentable sugars, if both pretreatment and enzymatic hydrolysis stages were considered. The highest total sugar yield of SBH and SBS using DA pretreatment and subsequently hydrolyzed with 30 FPU/g-DM cellulase were 86.9% and 70.3%, respectively.

Use of Unmanned Aerial Vehicle for Multi-temporal Monitoring of Soybean Vegetation Fraction

, 2016Purpose: The overall objective of this study was to evaluate the vegetation fraction of soybeans, grown under different cropping conditions using an unmanned aerial vehicle (UAV) equipped with a red, green, and blue (RGB) camera. Methods: Test plots were prepared based on different cropping treatments, i.e., soybean single-cropping, with and without herbicide application and soybean and barley-cover cropping, with and without herbicide application. The UAV flights were manually controlled using a remote flight controller on the ground, with 2.4 GHz radio frequency communication. For image pre-processing, the acquired images were pre-treated and georeferenced using a fisheye distortion removal function, and ground control points were collected using Google Maps. Tarpaulin panels of different colors were used to calibrate the multi-temporal images by converting the RGB digital number values into the RGB reflectance spectrum, utilizing a linear regression method. Excess Green (ExG) vegetation indices for each of the test plots were compared with the M-statistic method in order to quantitatively evaluate the greenness of soybean fields under different cropping systems. Results: The reflectance calibration methods used in the study showed high coefficients of determination, ranging from 0.8 to 0.9, indicating the feasibility of a linear regression fitting method for monitoring multi-temporal RGB images of soybean fields. As expected, the ExG vegetation indices changed according to different soybean growth stages, showing clear differences among the test plots with different cropping treatments in the early season of 1. Conclusion: Therefore, multi-temporal images obtained with an UAV and a RGB camera could be applied for quantifying overall vegetation fractions and crop growth status, and this information could contribute to determine proper treatments for the vegetation fraction.Keywords: Barley cover cropping, Excess green, Image processing, M-statistic method, UAV, Vegetation index

Biodegradable soy wound dressings with controlled release of antibiotics: Results from a guinea pig burn model

There is growing interest in the development of biodegradable materials from renewable biopolymers, such as soy protein, for biomedical applications. Soy protein is a major fraction of natural soybean and has the advantages of being economically competitive, biodegradable and biocompatible. It presents good water resistance as well as storage stability. In the current study, homogenous antibiotic-loaded soy protein films were cast from aqueous solutions. The antibiotic drug gentamicin was incorporated into the films in order to inhibit bacterial growth, and thus prevent or combat infection, upon its controlled release to the surrounding tissue. The current in vivo study of the dressing material in contaminated deep second-degree burn wounds in guinea pigs (n=20) demonstrated its ability to accelerate epithelialization with 71% epithelial coverage compared to an unloaded format of the soy material (62%) and a significant improved epithelial coverage as compared to the conventional dressing material (55%). Our new platform of antibiotic-eluting wound dressings is advantageous over currently used popular dressing materials that provide controlled release of silver ions, due to its gentamicin release profile, which is safer. Another advantage of our novel concept is that it is based on a biodegradable natural polymer and therefore does not require bandage changes and offers a potentially valuable and economic approach for treating burn-related infections.

Synergistic Functionality of Soybean 7S and 11S Fractions in Oil-in-Water Emulsions: Effect of Protein Heat Treatment

Oil-in-water emulsions stabilized by different concentrations of two fractions (7S and 11S) of soy protein, with or without heat treatment at 70 or 90°C, were investigated. Emulsions stabilized by 7S showed smaller droplets than those containing 11S. Moreover, emulsions stabilized by heat treated mixtures enriched in 11S produced gel after high pressure homogenization. Emulsions produced from 75% of 7S and 25% of 11S without or with heat treatment at 70°C showed the smallest droplets, indicating a synergistic effect between them. Thus, the combination between 7S and 11S has a great potential to be used as natural emulsifier in food-grade emulsions.

Selenomethionine and Total Methionine Ratio is Conserved in Seed Proteins of Selenium‐Treated and Nontreated Soybean, Flax, and Potato

ABSTRACTCrop biofortification with Se is widely accepted. However, little is known about the efficiency of selenomethionine (Se‐Met) incorporation into the seed and tuber protein fraction of crops. Here, we report on the efficiency of Se incorporation into the seed and tuber protein fraction of soybean, flax, and potato and the putative identity of some of the proteins that are likely targets for this random pretranslation modification. Soybean, flax, and potato plants received Se using different sodium selenate application methods and rates, and Se‐Met was determined in seed, tuber, and their protein fractions. Selenium content in seed and tuber tissues and in protein fractions was increased by 2 to 86 times. The methionine (Met) pool was increased, as was the proportion of Se‐Met in the Met pool, but the ratio of Se‐Met:total Met was well conserved (1:1) between the treated and nontreated plants. After two‐dimensional (2D) protein electrophoresis, five protein spots were identified as glycinin, trypsin inhibitor (soybean), patatin‐3‐Kuras‐1, patain‐B2, and phosphoenol carboxy kinase (potato), none of which showed S to Se substitution in their primary structure. Linking Se and three stress markers in fresh tuber extracts, glutathione (GSH) level, glutathione peroxidase (GPx), and glutathione reductase (GR) activities were increased by 20 to 35%. The data showed low protein modification in these non‐Se‐accumulator crops and indicated that rates of 5 to 10 g ha–1 Se, through foliar application, may ensure not only an adequate daily intake levels for humans but may also activate antioxidant enzyme systems within the tuber tissue.

Neural network and crop residue index multiband models for estimating crop residue cover from Landsat TM and ETM+ images

Crop residues on the soil surface provide not only a barrier against water and wind erosion, but they also contribute to improving soil organic matter content, infiltration, evaporation, temperature, and soil structure, among others. In Argentina, soybean (Glycine max (L.) Merill) and corn (Zea mays L.) are the most important crops. The objective of this work was to develop and evaluate two different types of model for estimating soybean and corn residue cover: neural networks (NN) and crop residue index multiband (CRIM) index, from Landsat images. Data of crop residue were acquired throughout the summer growing season in the central plains of Córdoba (Argentina) and used for training and validating the models. The CRIM, a linear mixing model of composite soil and residue, and the NN design, included reflectance and digital numbers from a combination of different TM bands to estimate the fractional residue cover. The results show that both methodologies are appropriate for estimating the residue cover from Landsat data. The best developed NN model yielded R2 = 0.95 when estimating soybean and corn residue cover fraction, whereas the best fit using CRIM yielded R2 = 0.87; in addition, this index is dependent on the soil and residue lines considered.

Assessment of adulteration of soybean proteins in dairy products by 2D microchip-CE device.

To determine the adulteration of soybean proteins in dairy product, a microchip-CE device was developed to isolate selected fraction of soybean and milk proteins in pI range from 5.5 ∼ 7.0 by 1D IEF, followed by ITP/CZE in the embedded capillary for preconcentration, separation and UV detection at 280 nm. Compared to IEF-CZE without ITP preconcentration, the enhancement factor (EF) in detection of soybean proteins was 20 times. Adulteration of 0.1% soybean protein in total dairy proteins can be detected in less than 10 min.

Thirteen week rodent feeding study with processed fractions from herbicide tolerant (DP-Ø73496-4) canola

The potential health effects of meal and oil processed from seed of genetically modified (GM) canola plants (OECD unique identifier: DP-Ø73496-4; hereafter referred to as 73496 canola) containing an insert that expresses the GAT4621 protein conferring tolerance to nonselective herbicidal ingredient glyphosate were evaluated in a subchronic rodent feeding study. Sprague–Dawley rats (12/sex/group) were administered diets containing dehulled, defatted toasted canola meal (DH meal) and refined/bleached/deodorized canola oil (RBD oil) processed from seed of plants that were untreated (73496), sprayed in-field with glyphosate (73496GLY), the non-transgenic near-isogenic (091; control), or one of four commercially available non-GM reference canola varieties (45H72, 45H73, 46A65, 44A89). All diets were formulated as a modification of the standard laboratory chow PMI® Nutrition International, LLC Certified Rodent LabDiet® 5002 (PMI® 5002). DH canola meal and RBD canola oil replaced all commodity soybean fractions typically incorporated in PMI® 5002. No toxicologically significant differences were observed between the test and control groups in this study. The results reported herein support the conclusion that DH meal and RBD oil processed from seed of 73496 canola are as safe and nutritious as DH meal and RBD oil processed from seed of non-GM canola.

Classification of Distinct Seed Carbohydrate Profiles in Soybean

Soybeans are an important source of protein-rich meal for livestock feed formulations. Recent changes in the cost of commodity-based sources of metabolizable energy (ME) inputs has put pressure on soybean meal to deliver both protein and ME in feed formulations. The non-oil fraction of soybean contains approximately 12% soluble carbohydrates, principally sucrose, raffinose, and stachyose. Of these carbohydrates, only sucrose is positive for ME. Both raffinose and stachyose, belonging to the raffinose family of oligosaccharides (RFOs), are considered antinutritional because of the negative consequences of their fermentation in the gut of monogastric animals when RFOs are consumed in the diet. Therefore, there is an interest in improving soybean seed composition so that it contains higher ME and fewer antinutritional components by increasing the sucrose content while lowering the RFOs. Several soybean lines have been discovered that contain altered levels of RFOs, and recent molecular genetic investigations have shown the phenotype to be caused by mutations in a raffinose synthase 2 (RS2) gene encoding the enzyme that is the committed step for RFO biosynthesis. The objective of this research was to determine the variation in carbohydrate profile for different soybean lines grown in a single location containing one of three different alleles of the RS2 gene. The results indicate that, although there is variation in the carbohydrate profiles for each line, different lines with the same RS2 genotype tend to produce a characteristic carbohydrate profile. Although the carbohydrate profile for each RS2 genotype class was consistent in different genetic backgrounds under two conditions grown at one location, more research will be necessary to determine the environmental stability of the carbohydrate profiles in multiple locations over different years.