Fourier Transform Infrared Spectroscopy Spectra Research Articles

Fe3O4-CdO Nanocomposite for Organic Dye Photocatalytic Degradation: Synthesis and Characterization

In this study, pure CdO nanoparticles, magnetic Fe3O4 nanoparticles, and Fe3O4-CdO nanocomposites were prepared via a solution combustion method using cetyltrimethylammonium bromide (CTAB) as a template. These prepared nanomaterial samples were characterized by X-ray diffraction (XRD), ultraviolet-visible diffuse reflectance spectroscopy (UV-vis DRS), Fourier-transform infrared spectroscopy (FTIR), Raman spectroscopy, X-ray photoelectron microscopy (XPS), transmittance electron microscopy (TEM), and scanning electron microscopy (SEM) analysis. XRD patterns confirmed the purity and the crystalline nature of the prepared samples. FTIR and Raman spectra observed the metal-oxygen (M-O) bond formation. UV-vis DRS studies were performed to investigate the optical properties and the bandgap energy determination. The surface morphology and the size of the pure CdO nanoparticles, magnetic Fe3O4, and nanocomposites of Fe3O4-CdO were determined via TEM and SEM analysis. Under optimum experimental conditions, the Fe3O4-CdO nanocomposites were applied for photocatalytic activity against Methylene blue dye. Under visible light irradiation, Fe3O4-CdO nanostructures showed an efficient photocatalytic degradation of 92% against Methylene blue organic dye and showed excellent stability for multiple cycles of reuse.

Asymmetric supercapacitor performance of hydrothermally-synthesized MWCNT-WO3 composite electrode

Supercapacitors are energy storage devices that have the potential to significantly contribute to meeting energy demands in the modern era. However, the charge storage capacity and stability of pseudocapacitive materials should be improved. To improve the electrode storage capacity, tungsten trioxide (WO3) has been synthesized and deposited on multi-walled carbon nanotubes (MWCNT-WO3) on stainless-steel substrates via hydrothermal technique. The structural, morphological, elemental, and compositional studies on WO3 and MWCNT-WO3 samples were done with X-ray diffractometry (XRD), field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDX), Fourier transform Infrared Spectroscopy (FTIR), contact angle, and Brunauer-Emmett-Teller (BET). The electrochemical features of the WO3 and MWCNT-WO3 materials were studied for supercapacitor application using a three-electrode set-up. The results obtained show highly crystalline films with porous nanoplates at the surface and monoclinic WO3 phase. The basic elemental constituents and chemical bonds present in the synthesized samples were confirmed using EDX and FTIR spectra. The MWCNT-WO3 exhibited an optimized capacitive behavior when compared to WO3. A specific capacitance of 818.31 Fg−1 was obtained at a scan rate of 5 mVs−1 in 0.5 M H2SO4 electrolyte. The composite MWCNT-WO3 was further used as an electrode in a solid-state asymmetric supercapacitor, PANI||MWCNT-WO3 fabricated with polyaniline (PANI) and MWCNT-WO3 as positive and negative electrodes in polyvinyl alcohol/sulphuric acid (PVA/H2SO4) gel electrolyte. The fabricated materials exhibited excellent electrochemical performance which makes them useful for supercapacitor applications.

Characteristics and evolution of bioethanol from Manila Tamarind (Pithecellobium dulce) leaf through fermentation

<p>This study's two primary goals are to synthesize bioethanol from biowaste and examine its thermal characteristics. Bioethanol must first undergo a comprehensive assessment of its thermal characteristics in order to be approved for usage in spark-ignition engines. A multi-step procedure comprising extraction, pretreatment, enzymatic hydrolysis, and fermentation was used to manufacture the bioethanol. The raw material was put through a preliminary screening using thermogravimetric analysis to find the mass loss rate as a function of temperature before to starting this process. Remarkably, the Manila tamarind leaves exhibited the highest mass loss, up to 34%. Enzymatic cellulose conversion to fermentable sugars was a critical step in the production of cellulosic ethanol. Following hydrolysis, Saccharomyces cerevisiae was employed in the fermentation process, leading to the bioethanol synthesis phase. The Manila Tamarind leaves yielded the most, around 29% by weight, which is amazing. In order to assess the thermal properties of the extracted ethanol, a variety of parameters were carefully examined, including the viscosity, density, cetane number, and calorific value. In each of these areas, mixtures of tire oil, gasoline, and bioethanol consistently outperformed the others. Furthermore, Fourier Transform Infrared Spectrometry spectra were utilized to validate the concentrations of potential groups, including alcohol, aromatic, alkyne, amide, and carbonyl groups. Clear objectives guide the fermentation process, aiming for consistent quality, safety, and functionality in the end product.</p>

Hydroxyapatite nanoparticles reinforced polyvinyl alcohol/chitosan blend for optical and energy storage applications

AbstractBiopolymer blend nanocomposite films composed of polyvinyl alcohol (PVA) and chitosan (CS) with varying quantities of hydroxyapatite (HA) nanoparticles were developed using a green solution casting method. The formation of blend nanocomposite was confirmed by Fourier‐transform infrared spectroscopy (FTIR), ultraviolet–visible (UV) spectrometer, field‐emission scanning electron microscopy (FE‐SEM), X‐ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). Mechanical parameters such as tensile strength, percentage elongation at break, hardness, dielectric constant, and temperature‐dependent alternating current (AC) conductivity were analyzed. The bonding between the nanoparticles and PVA/CS blend was verified by the shift in distinctive peaks of blend in the nanocomposites to a higher wavenumber in the FTIR spectra. The UV spectra demonstrated the difference in absorbance and transmittance of the PVA/CS blend with the reinforcement of HA nanofillers. The bandgaps of the nanocomposites marginally decreased with an increase in nanoparticle loading. The SEM and XRD patterns revealed the ordered arrangement of the blend matrix due to the presence of nanoparticles. The assessment of thermal characteristics obtained from DSC and TGA demonstrated a clear increase in phase transition temperature and thermal stability of nanocomposites. The electrical conductivity measurement indicated a rise in dielectric constant and AC conductivity of blend nanocomposites with the addition of nanoparticles. The temperature‐dependent AC conductivity showed a reduction in activation energy as temperature and nanoparticle loading increased. The tensile strength and surface hardness of the PVA/CS blend nanocomposite increased with the dosage of HA content in the blend matrix.Highlights A series of polyvinyl alcohol (PVA)/chitosan (CS)/hydroxyapatite (HA) nanocomposites were prepared by the green method Enhanced optical property, thermal stability, and glass transition temperature Possess excellent dielectric constant and alternating current conductivity The inclusion of HA increased the mechanical strength of the PVA/CS blend A potential blend nanocomposite for flexible optoelectronic and charge storage devices.

Study on preparation, structure, and cooling properties of TiO2/acrylonitrile–styrene–acrylate terpolymer solar reflective nanocomposite

AbstractPreparation, characterization, and cooling properties of TiO2 /acrylonitrile–styrene‐acrylate (ASA) terpolymer solar reflective nanocomposites with various contents of titanium dioxide (TiO2) nanoparticles were investigated. The formation of nanocomposites was monitored by Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and contact angle measurements. The cooling features were characterized via ultraviolet–visible–near infrared (UV–vis–NIR) spectrophotometry and heat build‐up tests. FTIR spectra show the disappearance of the vinylic absorption peak at 1636 cm−1 of the modified TiO2 which signifies good chemical interaction between the poly (butyl acrylate) chains and the nanoparticles. DLS measurements revealed relative enlargement in the diameter of nanocomposite latex particles from 107 to 184 nm as the percentage of mineral nanoparticles increased. SEM and TEM analysis revealed the formation of a core‐shell morphology with an almost uniform distribution of nanoparticles in the polymer chains. Spectral properties details obtained using UV–vis–NIR spectrophotometry revealed that surface modification of TiO2 nanoparticles resulted in a more than 7% increase in solar transmittance compared to the raw form. Nevertheless, grafting a thermoplastic shell onto the acrylic core reduced the average solar transmittance from 81.92% to 55.32%. Incorporating TiO2 nanoparticles (up to 6 wt%) into the polymer matrix led to an 18.44% further decrease in solar transmittance compared to the neat ASA. Heat build‐up tests showed enhanced cooling of 10.2, 12.7°C, 13.4°C, and 14.2°C for 0, 2, 4, and 6 wt% of used nanoparticles, respectively.Highlights In situ nanocomposites were synthesized through mini‐emulsion polymerization. Chemical bonding formed between functionalized TiO2 and polymeric matrix. ASA/TiO2 nanocomposite shields >56% of NIR light and 63% of solar light. Nanocomposites reduced heat build‐up temperature by more than 14°C during indoor test.

Structural, thermal and optical characteristics of Fe 1−X Zr X alloy by using mechanical milling approach: influence of Zr4+ ion

By changing Zr concentrations, Fe1-x-Zrx(x = 0.25 & 1 at%) alloys were successfully produced in an argon atmosphere using the mechanical alloying method. The produced Fe1-x-Zrxalloys were characterized by x-ray diffraction (XRD), transmission electron microscopy (TEM), energy dispersion x-ray analysis (EDAX), fourier transform infrared spectroscopy (FTIR), thermogravimetry analyzer (TG-DTA), ultraviolet-visible spectroscopy (UV–vis) absorption, and photoluminescence (PL) spectroscopy. According to XRD pattern analysis, the prepared alloys had a cubic crystalline structure and complete solid solution formation. The prepared alloy samples the average crystallite size was calculated using the Scherrer formula. The average crystallite size for the 0.25 at% Zr concentration is found to be 7.79 nm and 11.8 nm for the 1 at% Zr concentration. Lattice parameter changes are a very important tool for confirming the complete dissolution of the Zr atom in the Fe matrix.The TEM-dark field image confirms that the grain size is in the nanometric range (<100 nm). TEM-SAED spotty continuous ring pattern confirmed the complete solution formation is well correlated with the XRD results. The elemental composition of materials can be determined quantitatively through energy dispersive x-ray analysis in the Fe1-x-Zrxalloy. The elemental distribution of the mechanically alloyed samples shows that Zr elements are homogeneously distributed in the Fe matrix. Bands at 3428 cm−1 in the FTIR spectrum have been linked to O–H stretching vibrations. CH2 and CH stretching vibrations were associated with peaks of about 2920 cm−1 and 2850 cm−1. The weight loss and gain changes were observed and represented in the TG-DST graph; we found that overall weight changes are + 10.7% (gain) at 1023 °C for Fe 1-x-Zr x (x = 0.25 at%) alloy. However, when compared to 0.25 at% of Zr alloy, Fe-Zr (x = 1 at%) exhibits a low weight gain (+ 6.54%). The UV–visible absorbance edge revealed a blue shift when Zr was added, indicating alloy production. The energy band gap of materials was calculated using UV–vis, and it has been observed that the band gap reduces as Zr concentration increases. Zr was added to Fe1-x-Zrxalloy nanoparticles, resulting in 514 nm and 775 nm emission wavelengths. The greatest emission wavelengths and strong flawless sharp emission peaks were discovered to be between 450 nm and 550 nm. The higher PL emission peak was 514 nm at 0.25 at% of Zr.

A Novel 3-Dimensional Printed Nanoceramic Hybrid Resin Fixed Lingual Retainer: Characterization and Mechanical Tests.

Introduction: An innovative retention protocol was developed to create a new 3D-printed fixed retainer employing SprintRay OnX nanoceramic hybrid resin. The feasibility and usability of the retainer were subsequently evaluated. Methods: Identification and characterization of SprintRay OnX was done using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy with energy dispersive X-ray (SEM-EDX), field emission scanning electron microscope (FE-SEM), X-ray diffraction (XRD), and flexural strength. Load-deflection and pull-out tests were conducted on the 3D-printed straight wires, with three distinct cross-sectional geometries: round (1 mm), oval (1 mm × 1.5 mm) and semielliptical (1 mm × 1.5 mm). Twisted G&H and coaxial Respond stainless steel multistrand retainers were used for comparison. In the load-deflection test, a three-point bending test (3PBT) was employed. For the pull-out test, the retainer wire was inserted into the composite, which was placed in a centrally located hole of an acrylic block; the retainer wire was subjected to a tensile force along its long axis. Results: Characteristic bands close to those of PMMA were observed in the FTIR spectra. SEM-EDX and XRD revealed a crystalline material with homogeneously distributed Yb element signals (19.4%). On FE-SEM micrographs, small clumps were displayed on smooth surfaces. The flexural strength and the flexural modulus were, respectively, 142.48 MPa and 7.842 GPa. All groups of 3D-printed wires exhibited significantly higher load-deflection levels than the multistrand wires (MSWs). Concerning pull-out forces, they fell in between twisted G&H (96 N) and coaxial Respond (48.09 N) retainer wires. The 3D-printed wires fractured cohesively without detachment from the adhesive, suggesting that the chemical bond was adequate for satisfactory wire integration, yet the wire's strength was compromised. Concerning the cross-sectional geometry, the load-deflection and the pull-out forces of 3D-printed oval and semielliptical wires were significantly higher than that of 3D-printed round wires, which was attributed to the larger cross-sections of the wires. Conclusion: Oval and semielliptical 3D-printed wires offered favorable features as lingual retainers.

Repeated mechanical recycling of biodegradable polymers: PLA exhibits less deterioration than PBAT/PLA blend

Biodegradable polymers have been extensively researched as alternatives to non-biodegradable fossil-based polymers. Although they reduce environmental impact, their production competes with land needed for food crops. Therefore, exploring their reuse and recycling is essential for enhancing their circular economy and sustainability. This study evaluated the recyclability of commercially available biodegradable polymers, poly (lactic acid) (PLA) and its blend with poly (butylene adipate co-terephthalate) (PBAT), across three cycles of mechanical recycling. Each cycle simulates plastic production, shelf-life, washing, and reprocessing stages. Samples were analysed after molding, ageing, and washing for each cycle using Differential Scanning Calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), tensile testing, and rheological analysis to track changes in crystallization, chemical structure, viscosity, and mechanical behaviors. Results indicated that both PLA and PBAT/PLA (55/45 wt%) blend showed a rise in crystallinity (Xc) due to the annealing effect of the ageing and washing. No changes in FTIR spectra and Xc were detected after one recycling cycle, indicating stability. After three reprocessing cycles, second heating crystallinity rose from 2 % to 13 % for PLA and from 2 % to 12 % for PBAT/PLA (45 wt% PLA), indicating morphological changes from degradation and molecular weight reduction. Despite higher crystallinity and a more moderate decline in complex viscosity and storage modulus than PLA, PBAT/PLA showed reduced mechanical properties, with a 90 % drop in elongation at break and nearly 50 % in stress at break, highlighting the need for interventions to control PBAT degradation. PLA maintained strong mechanical properties, demonstrating its potential as a compostable recyclable material.

Agar-gelatin-derived hydrogel-based controlled delivery devices for linuron herbicide to prevent environmental hazards

Innovation in agriculture chemicals and delivery systems is essential for the production of food for sustainable development. Herein, natural polymers and agar-gelatin-derived controlled delivery devices for linuron herbicide to prevent environmental and health hazards. These hydrogels were designed by the graft-copolymerization reaction of poly(methacrylamide) [poly(MAAm)] on agar-gelatin for use as controlled-release formulations (CRF). Copolymers were characterized by Copolymers were characterized scanning electron micrographs (SEM), Atomic force microscopy (AFM), X-ray diffraction (XRD) Fourier transform infrared spectroscopy (FTIR), 13C-nuclear magnetic resonance (NMR), and thermogravimetric analysis -differential thermogravimetry (TGA-DTG) techniques. SEM shows the uneven surface morphology, and AFM showed a rough surface with an average roughness value of 137.50 nm in the polymeric matrix. The amorphous nature of the polymer was confirmed with XRD techniques. Grafting of the poly(MAAm) on the agar and gelatin was confirmed by FTIR and 13C NMR spectra. Agar-gelatin-cl-poly(MAAm) hydrogels absorbed water at 4.05 ± 0.32 g per gram, which is useful for soil water retention. Release of the linuron herbicide occurred in a controlled manner during 72 h of observations and followed non-Fickian diffusion mechanism. The slow release of the linuron from the hydrogel is a useful observation for preventing leaching and evaporation loss of herbicide. In the soil column, the release of linuron occurred in a slow and sustained manner for 240 h. A soil adsorption study showed a decrease in ground water ubiquity score (GUS) for linuron-encapsulated hydrogel as compared to commercial formulations. It can reduce groundwater contamination risk due to the leaching of the linuron herbicide. Degradation of CRF may improve micronutrient and soil quality.

Synthesis, Characterization and Antimicrobial Properties of Cellulose from Pentaclethra macrophylla Benth pod (PMBP)

Pentaclethra macrophylla Benth pod (PMBP) popularly known as African oil bean pod is a known bio-waste carelessly littered around the eastern part of Nigeria, adding to pollution. The purpose of this research was to synthesize cellulose from Pentaclethra macrophylla Benth pod (CPMBP), characterise and assess the anti-microbial activity. Fourier Transform Infrared Spectroscopy (FTIR) analysis was carried out on the synthesized cellulose at Central Laboratory, University of Nigeria Nsukka and was discovered to have a signal at 897.92 cm-1 in all spectra denoting the glycosidic linkages of the glucose ring in cellulose. The X-ray Diffraction (XRD) analysis was carried out on the synthesized cellulose at Nano Laboratory, University of Nigeria Nsukka and was found that pure cellulose, cellulose nanocrystals and Cellulose from Pentaclethra macrophylla Benth pod (CPMBP), had typical peaks at 2θ values of 15o and 22.6o, thereby showing that CPMBP is a form of cellulose. In the XRD diffractogram, pure cellulose had sharpest peak at around 2θ＝22.6o while that of CPMBP was sharpest at around 2θ＝22.4o. Scanning Electron Microscopy (SEM) analysis was performed at Central Laboratory kwara state and images of synthesized cellulose from PMBP powder at 1000x magnification was aimed at assessing the shape (morphology) and elemental composition of the synthesized cellulose of PMBP biomass. The existence of cellulose in PMBP biomass was confirmed by the overall FTIR, SEM and XRD spectrum, along with traces of lignin and hemicelluloses. The sensitivity test of the cellulose extract was carried out against 3 different test microorganisms (Salmonella typhi, Candida albicans, and Aspergillus niger) at the concentration of 10 mg/ml of the agent. The rest result showed that Salmonella typhi, and Candida albicans were sensitive to the agent at 10 mg/ml concentration whereas Aspergillus niger was resistant to the agent. This research has shown that two important disease-causing bacteria and fungi that affect the human population severely may be inhibited by the potential of the cellulose extract at 10 mg/ml concentrations and thus be useful in the Pharmaceutical industry.

Physiochemical and functional properties of Moringa oleifera protein obtained by isoelectric precipitation

The work investigated the effect of extraction pH (10 or 11) and precipitation pH (4 or 4.5) on the physicochemical and functional properties of Moringa oleifera protein. The physicochemical characteristics of Moringa leaf powder were analyzed using proximate quantification based on AOAC standard methods. The thermal, spectral, x-ray diffraction data were collected using the differential scanning calorimetric, Fourier transform infrared spectroscopy (FTIR), and wide-angle x-ray diffraction. The extraction at pH 10 and the precipitation process at pH 4.5 generated the highest protein content of 58.88%. Some of the functional properties of this pH combination were emulsion activity at 29.67%, emulsion stability at 50.45%, protein solubility at 32.36%, and water absorption capacity of 4.78 mL water/g. The thermal properties suggested the denaturation temperature of Moringa protein powder at 75°C, which was comparable to that previously reported plantbased protein isolate. FTIR and x-ray spectra argued the domination of amorphous protein fraction with a trace of lipid and carbohydrate. With these characteristics, protein has great potential to be used for food and drug applications.

Single and Double Microencapsulation of Organic Thermochromic Material

With the increase in temperature, urban areas face issues like urban heat islands. The reduction of green vegetation across urban areas has led to a tremendous rise in temperature. Many studies suggest significant cities are currently experiencing this issue to a greater extent. Many researchers are working on building paints and dyes to reduce the heat intake of the building. Organic thermochromic materials are one such alternative for building dye and stain. The main issue with using organic thermochromic materials is that they quickly degrade under sunlight, which makes them less useful for building paint. To overcome this issue, many organic thermochromic black dye materials (OTCBDM) are encapsulated with inorganic materials such as metal oxide, which block sunlight and reduce the effect of degradation. Encapsulation is achieved via the emulsification method of metal oxide. This paper deals with the single and double microencapsulation methods with different surfactant concentrations, metal oxides, and combinations thereof. Various techniques were used such as Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) with energy‐dispersive X‐ray spectroscopy technique (EDS), and differential scanning calorimeter (DSC), UV‐Vis spectroscopy, thermogravimetric analysis (TGA), and CIE Lab space analysis to determine the materials’ encapsulation and chromatic nature. The FTIR spectra suggested the absorption peaks for the Ti─OH bond (at 814, 903, and 913 cm−1) and Si─OH group (at 1,084 and 1,178 cm−1) for the commercial dye encapsulated with TiO2 and SiO2. The Ti─O─Si bonds at 1,054 and 1,145 cm−1 showed for the double encapsulation of SiO2 and TiO2 either (i) doped or in (ii) layered structures. From the EDS analysis, we can clearly say that the presence of SiO2 and TiO2 indicates the encapsulation of the commercial thermochromic black dye material. The SEM micrographs of the TiO2 encapsulation of OTCBDM seem more promising and uniform core–shell structure formation with CTAB as a surfactant than the SDBS. Moreover, the SEM microstructure results of double encapsulation using both TiO2 and SiO2 suggested thicker coating of particles when compared with single encapsulated counterparts. The cold phase of layered double encapsulation of OTCBDM + SiO2/TiO2 shows a darker color than the doped combination as obtained from the CIE Lab results. This feature suggested that an efficient encapsulation and chromatic transition are achieved in a layered combination of double encapsulation than in a doped combination. The DSC spectra indicated the increase in enthalpy at the heat cycle from 94.147 J/g for the SiO2 microencapsulated OTCBDM sample compared to all other samples shows thermal energy storage behavior for outdoor building applications. The UV–Vis absorbance spectra show two distinct peaks at 470 and at 610 nm related to the OTCBDM. The single‐layer encapsulated organic thermochromic black dye material shows less absorbance compared to the plain organic thermochromic black dye material. The double encapsulated sample has the highest absorbance compared to all other samples suggesting the material having better retention of peaks at 470 and at 610 nm. The thermographic analysis (TGA) shows when compared to the plain and single encapsulated OTCBDM samples, the double encapsulated samples demonstrated higher thermal endurance characteristics due to thicker layer coatings of the shell over the core structure. The novelty of this work relies on the double encapsulation of OTCBDMs using SiO2 and TiO2 to improve the photodegradation behavior of the organic core dye particles, and we have demonstrated that the cationic surfactant CTAB outperforms than the SDBS in microencapsulation processes.

Anti-plasticizing effect of water on prilocaine and lidocaine - the role of the hydrogen bonding pattern.

It is generally accepted that water, as an effective plasticizer, decreases the glass transition temperatures (Tgs) of amorphous drugs, potentially resulting in physical instabilities. However, recent studies suggest that water can also increase the Tgs of the amorphous forms of the drugs prilocaine (PRL) and lidocaine (LID), thus acting as an anti-plasticizer. To further understand the nature of the anti-plasticizing effect of water, interactions with different solvents and the resulting structural features of PRL and LID were investigated by Fourier transform infrared spectroscopy (FTIR) and quantum chemical simulations. Heavy water (deuterium oxides) was chosen as a solvent, as the deuterium and hydrogen atoms are electronically identical. It was found that substituting hydrogen with deuterium showed a minimal impact on the anti-plasticization of water on PRL. Ethanol and ethylene glycol were chosen as solvents to compare the hydrogen bonding patterns occurring between the hydroxyl groups of the solvents and PRL and LID. Comparison of the various Tgs showed a weaker anti-plasticizing potential of these two solvents on PRL and LID. The frequency shifts of the amide CO groups of PRL and LID due to the interactions with water, heavy water, ethanol, and ethylene glycol as observed in the FTIR spectra showed a correlation with the binding energies calculated by quantum chemical simulations. Overall, this study showed that the combination of weak hydrogen bonding and strong electrostatic contributions in hydrated PRL and LID could play an important role in inducing the anti-plasticizing effect of water on those drugs.

Development of Polymeric Micelles for Combined Delivery of Luteolin and Doxorubicin for Cancer Therapy.

Background: Luteolin (LUT) is a bioactive compound with several pharmacological activities including anticancer effect. Doxorubicin (DOX) is an anthracycline chemotherapeutic drug that have proven to be effective in treating various types of cancers. Polymeric micelles (PMs) containing biologically active materials have emerged as prospective dosage forms with high drug-loading, which can add therapeutic benefit to the poorly water-soluble compounds and novel chemical entities. PMs are effective in delivering several drugs, such as anticancer drugs, antifungal drugs, flavonoids and drugs targeting the brain. The aim of the current study is to develop PMs for LUT and DOX as a combined delivery system for cancer therapy. Methods: PMs were prepared using 2.5% of each of LUT and DOX with varying compositions of Poloxamer 188, Poloxamer 407, Vitamin E (TPGS), Poloxamer 123 and Gellucire 44/14 at room temperature. Particle size, polydispersity index, zeta potential, were achieved using Zetasizer Nano particle size analyzer and the sizes were further confirmed with transmission electron microscopy (TEM). Prepared PMs were further characterized using powder X-ray diffraction (PXRD) and fourier transform infrared spectroscopy (FTIR). An MTT assay was performed on breast cancer (MCF-7) cells and liver cancer (HepG2) cells to determine the cytotoxic effect of the different PMs formulations. Results: PMs were successfully developed and optimized using 74.3% Poloxamer 407 with 20.7% Vitamin E (TPGS), and 70% Poloxamer 407 with 25% Gellucire 44/14, respectively. The droplet size and polydispersity index were found to be 62.03 ± 3.99 nm, 91.96 ± 5.80 nm and 0.33 ± 0.05, 0.59± 0.03, respectively for PMs containing TPGS and Gellucire 44/14. Zeta potentials of the PMs containing TPGS and Gellucire 44/14 were recorded as -2.27 ±0.11mV and -7.78 ± 0.10 mV, respectively. The PMs showed a spherical structure with approximately 50-90 nm range evident by TEM analysis. The PXRD spectra of PMs powder presented the amorphization of LUT and DOX. The FTIR spectra of LUT-loaded and DOX-loaded PMs were identical, suggesting consistent PMs composition. The MTT assay showed that the representative combined drug loaded PMs treatment led to a reduction in the viability of MCF-7 and HepG2 cells compared to drug free PMs and pure LUT, DOX alone. Conclusions: PMs with LUT and DOX exhibited significant cytotoxic effects against breast and liver cancer cells and could thus be an important new pharmaceutical formulation to treat cancer.

Evaluation of Physicochemical Changes in Hard-Boiled Eggs Stored at Different Temperatures.

Eggs that have been hard-boiled are frequently used as ready-to-eat food. Refrigerated and frozen storage of hard-boiled eggs causes issues, such as customer rejection owing to textural changes. The objective of this research is to ascertain how storage temperature affects hard-boiled eggs' alteration in texture over time. Medium-sized brown shell eggs were acquired from a local market, boiled at 100°C for 15 min, and then stored at room temperature (25°C), refrigeration (4°C), and freezing (-18°C) conditions for 0, 12, 24, and 48 h. Fourier transform infrared spectroscopy (FTIR), texture profile, visual observation using a gemological microscope, free amino acid content, and color were measured. Freezing had a substantial impact on the eggs' hardness, gumminess, chewiness, and cohesiveness (p<0.05). The FTIR spectrums confirmed the textural changes in bonds of amide A (3,271 cm-1), amide I (1,626.2 cm-1), amide II (1,539.0 cm-1), C=O stretch of COO- (1,397 cm-1), asymmetric PO2- stretch (1,240 cm-1). Microscopic images confirmed structural changes in eggs stored at -18°C. The free amino acid content was lower in fresh and frozen eggs than in the rest (p<0.05). However, there was no discernible variation in the egg white's color when eggs were kept at 4°C (p>0.05). Salmonella spp. was found exclusively in eggs kept at room temperature. In conclusion, hard-boiled eggs did not exhibit structural or chemical changes when stored at 4°C for up to 48 h compared to freezing and room temperature conditions.

Sustainable green synthesis of Hedychium coronarium leaf extract-stabilized silver nanoparticles and their applications: colorimetric sensing of Sn2+ and Hg2+ and antifungal and antimicrobial properties.

Hedychium coronarium (Hc) (commonly known as Gulbakawali) leaf extract was used for the stable and sustainable green synthesis of silver nanoparticles (Hc-AgNPs), which were biodegradable and non-toxic. Hedychium coronarium leaf extract was used as a reducing agent to stabilize the Hc-AgNPs by converting Ag+ to Ag0 without adding any capping agent. It demonstrated stability for up to six months, and no agglomeration was observed. The Hc-AgNPs were characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), ultraviolet-visible spectrophotometry, and fluorescence spectroscopy analysis. The UV-visible spectrum supported the formation of stable Hc-AgNPs by displaying a strong surface plasmon resonance (SPR) peak at 440 nm. FT-IR spectra showed the functional groups present in the leaf extract of Hedychium coronarium, which was the primary source of secondary metabolites attached to Ag0. XRD analysis revealed a distinct 2θ peak of Hc-AgNPs at 38.15°, indicating a face-centred cubic structure with a crystallite size of 22.6 ± 1 nm at the (111) plane. Moreover, TEM demonstrated the spherical morphology of the Hc-AgNPs with an average particle size of 22.42 ± 1 nm. The photophysical characteristics of the Hc-AgNPs, as highlighted by their UV-vis and fluorescence characteristics, revealed their semiconducting nature with an impressive band gap (E g) value of 3.78 eV. Fascinatingly, the fluorescence activity of Hc-AgNPs at 504 nm showed a broad emission band corresponding to the absorption band at 251 nm. We performed the selective colorimetric sensing of Sn2+ metal ions using Hc-AgNPs, which demonstrated a detection limit of 10-3 M, suggesting their potential as very good solid biosensors. Interestingly, the Hc-AgNPs showed antifungal activity, which has not been reported before. Specifically, the results showed that the Hc-AgNPs had a higher fungitoxicity effect against Aspergillus flavus (59.58 ± 3.68) than against Fusarium oxysporum (57.93 ± 4.18). The antibacterial activity of the Hc-AgNPs was evaluated against three Gram-negative phytopathogenic bacteria: Xanthomonas oryzae (X. oryzae), Ralstonia solanacearum (R. solanacearum), and Erwinia carotovora (E. carotovora), showing effective inhibition zones of 16.33 ± 0.57, 15.33 ± 0.57, and 14.33 ± 0.57 mm, respectively. These results indicate that the Hc-AgNPs could inhibit these phytopathogenic bacteria with varying degrees of effectiveness in the order of X. oryzae > R. solanacearum > E. carotovora.

Rifampicin adsorption and release study using Santa Barbara amorphous-16 modified Al (SBA-16-Al) for a drug delivery system.

In this study, the surface modification of Santa Barbara Amorphous-16 (SBA-16) with aluminum (SBA-16-Al) was carried out as a rifampicin matrix for the treatment of tuberculosis. Surface modification of SBA-16 was achieved using the direct-synthesis grafting method. Then, the adsorption and release properties of rifampicin from the SBA-16-Al matrix have been studied in batches. In addition, the SBA-16-Al has been characterized using Fourier-Transform Infrared Spectroscopy (FTIR), X-ray diffraction analysis (XRD), transmission electron microscopy (TEM), and Surface Area Analysis (SAA) Brunaur, Emmett and Teller (SAA-BET). The results show that the mesoporous material, the SBA-16-Al has a specific surface area of 843.5 m2 g-1 and 624.3 m2 g-1 for SBA-16, nanometer-sized pore diameters, and an amorphous crystal lattice. The FTIR spectra showed the Al-O bond at 802 cm-1 which indicates the Al group has been successfully added into SBA-16. The adsorption isotherm of rifampicin in SBA-16-Al follows the Freundlich model which illustrates the adsorption is heterogeneous and forms a multilayer. The adsorption of rifampicin is chemisorption which occurs non-spontaneously and is quite stable. The release kinetics of rifampicin in the drug delivery system followed the Higuchi model with k1 0.5472 mg 0.5/hour pH 1.5 and k2 mg 0.5/hour pH 6.5.

Mass scale production and purification of graphite oxide from Sri Lankan vein graphite and spectroscopic characterization

Discovery of graphene has enhanced attention on industrial scale production of graphene using natural graphite which involves oxidation followed by reduction processes. Aiming for the first time, mass scale production of graphite oxide from Sri Lankan vein graphite of natural purity 99.5% carbon, following an improved Hummer’s method was experimented at optimized conditions minimizing chemical, energy and time wastage. The present study further aimed at determination of pH and manganese ions on successive purification processes of graphite oxide. The X-ray diffraction spectroscopy (XRD), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) characterizations were followed for verification of products. The wastewater produced from graphite oxide preparation process was systematically tested for Mn2+ ion using Atomic Absorption Spectroscopy (AAS). XRD peaks verified the formation of graphite oxide successfully through a complete oxidation of graphite. FTIR spectrum exhibited characteristic peaks related to typical graphite oxide while SEM shows the typical morphological features. XPS analysis verified complete removal of Mn from graphite oxide after purification. AAS analysis reveals entire removal of Mn after several washing cycles using only water. The investigation concludes that even mass scale production of quality graphite oxide is possible from Sri Lankan pure vein graphite which can subsequently be used to produce precious graphene and derivatives for various high-end applications.

Functional Group and Phytochemical Properties Detection of Peperomia Pellucida Leaves Extract

Since prehistoric times, medicinal plants, often known as medicinal herbs, have been identified and employed in traditional healing practises. Because of the existence of phytochemical ingredients, medicinal plants synthesise hundreds of chemical compounds and are a key source of molecules with therapeutic qualities. Wild herb leaves were taken from the green region of Universiti Sains Islam Malaysia, specifically Peperomia pellucida, also known as 'sireh cina' in Malaysia. The dried wild herb leaves were crushed. For 48 hours, the powdered materials were treated to a solid-liquid extraction method utilising hot water and ethyl acetate as a solvent. For all examined samples, a phytochemical screening test of hot water and ethanolic leaves extract confirmed the presence of antioxidant chemicals such as flavonoid and phenolics. Meanwhile, resins were only found in ethanolic leaves extract, and steroid was not found in any of the studied samples. Fourier-Transform Infrared Spectroscopy (FTIR) was used to study the organic component in greater depth by evaluating the chemical ingredients in the crude extracted samples of ethyl acetate. The study of FTIR spectra revealed that the hydroxyl group, alkanes, alkyl groups, benzoic compounds, and phenols were the primary functional groups. The identification of functional groups indicates a possible source of antioxidants and highlights a potential prospect for the development of natural products from these wild plants in the drug discovery business. Disc diffusion analysis of antibacterial properties exhibited significant clear zone formation against Gram-positive and Gram-negative tested bacteria.

Prospective Antiviral Effect of Ulva lactuca Aqueous Extract against COVID-19 Infection.

Marine algal extracts exhibit a potent inhibitory effect against several enveloped and non-enveloped viruses. The infection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has several adverse effects, including an increased mortality rate. The anti-COVID-19 agents are still limited; this issue requires exploring novel, effective anti-SARS-CoV-2 therapeutic approaches. This study investigated the antiviral activity of an aqueous extract of Ulva lactuca, which was collected from the Gulf of Suez, Egypt. The aqueous extract of Ulva lactuca was characterized by high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and Energy Dispersive X-ray (EDX) analyses. According to the HPLC analysis, the extract comprises several sugars, mostly rhamnose (32.88%). The FTIR spectra showed numerous bands related to the functional groups. EDX analysis confirmed the presence of different elements, such as oxygen (O), carbon (C), sulfur (S), magnesium (Mg), potassium (K), calcium (Ca), and sodium (Na), with different concentrations. The aqueous extract of U. lactuca (0.0312 mg/mL) exhibited potent anti-SARS-CoV-2 activity via virucidal activity, inhibition of viral replication, and interference with viral adsorption (% inhibitions of 64%, 33.3%, and 31.1%, respectively). Consequently, ulvan could be a promising compound for preclinical study in the drug development process to combat SARS-CoV-2.