You have accessJournal of UrologyStone Disease: Basic Research II1 Apr 2015MP34-02 GENE EXPRESSION PROFILE OF RANDALL'S PLAQUE IN IDIOPATHIC CAOX STONE FORMERS SUGGESTS DYSREGULATION OF M&[PHIS]-RELATED GENES Kazumi Taguchi, Rei Unno, Yasuhiro Fujii, Taku Naiki, Shuzo Hamamoto, Ryosuke Ando, Kazuhiro Kanemoto, Atsushi Okada, Takahiro Yasui, Keiichi Tozawa, Yutaro Hayashi, and Kenjiro Kohri Kazumi TaguchiKazumi Taguchi More articles by this author , Rei UnnoRei Unno More articles by this author , Yasuhiro FujiiYasuhiro Fujii More articles by this author , Taku NaikiTaku Naiki More articles by this author , Shuzo HamamotoShuzo Hamamoto More articles by this author , Ryosuke AndoRyosuke Ando More articles by this author , Kazuhiro KanemotoKazuhiro Kanemoto More articles by this author , Atsushi OkadaAtsushi Okada More articles by this author , Takahiro YasuiTakahiro Yasui More articles by this author , Keiichi TozawaKeiichi Tozawa More articles by this author , Yutaro HayashiYutaro Hayashi More articles by this author , and Kenjiro KohriKenjiro Kohri More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.1298AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Randall's plaque (RP) is the origin of renal calcification on which idiopathic calcium oxalate (CaOx) kidney stones develop. Despite numerous investigations with animal hyperoxaluric stone models and human samples, the exact role of RP in the formation of CaOx crystals remains unknown. We recently reported that osteopontin (OPN) expression and macrophage (M&[phis]) migration around crystals were essential for stone formation in a transgenic mouse stone model, suggesting the potential therapeutic effect of these molecules. Hence, we examined the gene expression profile in RP sections from human papillary tissues and studied the controlling factors for the development of RP. METHODS We obtained biopsies of renal papillary tissues from 20 idiopathic CaOx stone patients who had undergone retrograde intrarenal surgery in our institution from November 2013 to October 2014. Samples were individually collected from 2 different regions in each patient, renal papillary mucosa with RP (RP group) and normal papillary mucosa without RP (normal group). We also collected normal renal papillary mucosa as a control group from 5 patients with non-CaOx stones undergoing ureteroscopy for screening. We performed immunohistochemical stains and transmission electron microscopy (TEM) in order to evaluate the ultra-microstructure of RP. We analyzed the gene expression profile in RP, normal, and control groups using microarray. Finally, we performed PCR to compare the expression of OPN and M&[phis]-related genes among the 3 groups. RESULTS X-ray diffraction showed that RP contained deposits of calcium phosphate sodium. TEM revealed basement membranes surrounding RP and the local expression of OPN and collagen. Gene ontology analysis demonstrated that the RP group had markedly higher gene expressions related to endochondral bone morphogenesis, morphogen/motor activity, Ca++ binding activity, and upregulation of inflammation. Whereas lower gene expressions related to oxygen transport, antioxidant activity, chemokine receptor activity, and acute immune/inflammatory response were noted compared to the control group. Inflammatory M&[phis]-related gene expressions, such as IL-6 and MCP-1, were significantly higher, but the expression of the CCR2 gene, which is related to anti-inflammatory M&[phis] activity, was lower in the RP group than in the normal or control groups. CONCLUSIONS Our results provide compelling evidence that dysfunctions of the antioxidant, inflammatory, and immune response cause the development of RPs and that M&[phis]-related genes may regulate their genesis. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e410-e411 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Kazumi Taguchi More articles by this author Rei Unno More articles by this author Yasuhiro Fujii More articles by this author Taku Naiki More articles by this author Shuzo Hamamoto More articles by this author Ryosuke Ando More articles by this author Kazuhiro Kanemoto More articles by this author Atsushi Okada More articles by this author Takahiro Yasui More articles by this author Keiichi Tozawa More articles by this author Yutaro Hayashi More articles by this author Kenjiro Kohri More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...