Fluoride Dentifrice Research Articles

Relationship between 1-, 3-, and 6-Month Gingival Bleeding Outcomes.

Results from systematic reviews and meta-analyses show generally consistent antigingivitis effects between 3- and 6-mo observation time points with twice-daily use of stannous fluoride (SnF2) dentifrice. However, the relationship between 1-, 3-, and 6-mo gingivitis responses has not been investigated. This pooled analysis was conducted to understand the relationship of 1-, 3-, and 6-mo gingival bleeding outcomes. Number of bleeding sites, derived from Löe-Silness Gingival Index (LSGI) or Gingival Bleeding Index, was identified as the primary end point of the analysis for the biological and clinical relevance. Randomized, double-blinded, controlled clinical studies meeting the following predefined selection criteria were identified: 1) published and unpublished gingivitis clinical trials conducted from 1995 to 2022 comparing efficacy of 0.454% SnF2 dentifrices to negative controls (sodium fluoride or sodium monofluorophosphate dentifrice) and 2) studies with a 3-mo assessment and at least a 1- or 6-mo assessment. The search resulted in ten 6-mo and fourteen 3-mo studies meeting selection criteria. A mixed-effects model was performed on the pooled data to assess gingival bleeding outcomes across time. The bleeding efficacy significantly increased between months 1 and 3 (P < 0.0001) and plateaued between months 3 and 6 (P = 0.007), supporting the fact that bleeding reduction relative to control established by 1 mo will increase and be maintained through 3 and 6 mo (R2 = 0.857). In addition, gingival bleeding and gingivitis efficacy, as measured by LSGI, were found to be highly correlated (R2 = 0.874). A clear relationship has been demonstrated between 1-, 3-, and 6-mo gingival bleeding outcomes in gingivitis clinical studies comparing SnF2 dentifrice to negative control dentifrice. These findings have important implications to the dental practice and scientific research as antigingivitis efficacy evaluations can be observed as early as 1 mo and are consistent with those seen at 3 or 6 mo. Outcomes from this investigation indicate that the clinical evaluation of antigingivitis efficacy at 1 mo is predictive of that at 3 and 6 mo, supporting studies of 1-mo duration as a viable method of knowledge acquisition. This more efficient, expedited research design has positive implications for patient care, clinical practice guidelines, protocols, and policies.

Effects of a stabilized stannous fluoride dentifrice on clinical, immunomodulatory, and microbial outcomes in a human experimental gingivitis model.

Stannous fluoride dentifrice is well established for its beneficial clinical effects. In this study, we evaluated the effects of stannous fluoride on inflammation and oral microbiome. In this randomized, parallel-arm, double-blind, controlled clinical trial, we compared clinical resolution of experimental gingivitis by evaluating bleeding on probing, gingival index, and plaque index between stannous fluoride stabilized with zinc phosphate (test) and sodium fluoride (control) dentifrices. Further, these groups were compared for oral neutrophil counts, systemic priming of neutrophils, gingival crevicular fluid (GCF) expression of inflammatory markers, and the oral microbiome. We found significant reduction in bleeding on probing in the test group compared to the control group in experimental gingivitis when participants used the test dentifrice prior to induction of experimental gingivitis. The test group also showed significant reductions in GCF levels of inflammatory markers (matrix metalloproteinase 8 [MMP8], receptor activator of nuclear factor kappa-Β ligand [RANKL]), oral polymorphonuclear neutrophil (PMN) counts, and systemic neutrophil priming (CD11b expression) during experimental gingivitis. Further, significant reductions in the gram-negative genera Porphyromonas, Tannerella, and Treponema were noted in the test group. The stannous fluoride stabilized with zinc phosphate dentifrice formulation demonstrated clinical reduction in gingival inflammation and a beneficial effect on microbiome and immune markers. This intervention should be explored as a preventive aid in the progression of plaque-induced gingivitis to periodontitis.

Effect of Tooth Brushing Cycles and Dentifrice Fluoride Concentration on a Glazed CAD/CAM Ceramic.

To evaluate the effect of tooth brushing and dentifrice fluoride (F-) concentration on changes in color and translucency (ΔE00 and ΔT00, respectively), surface gloss (GS), surface roughness (Sa), and microstructure of a glazed CAD/CAM ceramic. Ceramic blocks (e.max/CAD) were sectioned into rectangular plates (14 x 12 x 1 mm), and one surface of each sample was glazed. Samples were divided into three groups according to the F- concentration in the dentifrice (0, 1,100, and 5,000 μg/g) and were then subjected to 60,000 tooth brushing cycles. Luminosity and color were measured using a spectrophotometer at baseline and after every 20,000 cycles to obtain their ΔE00 and ΔT00 values. Another set of samples was prepared to measure the GS with a gloss meter and the Sa with a confocal laser microscope. The GS and Sa results were subjected to analysis of variance, Tukey test, and Dunnett test (α = .05). After 60,000 tooth brushing cycles, all of the variables were clinically acceptable, and there were no significant differences in the ΔE00, ΔT00, GS, or Sa among the fluoridated dentifrices. The GS values decreased significantly as the number of tooth brushing cycles increased. The ΔE00, ΔT00, GS, and Sa values were all clinically acceptable after the glazed e.max/CAD ceramic had been subjected to 60,000 tooth brushing cycles with dentifrices containing up to 5,000 μg/g of F-.

Comparative Evaluation of Remineralization and Antimicrobial Efficacy of Maximum Fluoride Containing Toothpastes Available in India - In-Vivo Study

Background: Early childhood caries (ECC) is a common type of dental caries with an overall prevalence of 49.6% in India. Dentifrices- containing 1000-1500ppm concentration of fluorides and certain remineralizing agents are known to be effective in reversal of early enamel lesions and reduction of dental caries prevalence. However, in India, the mean fluoride content of dentifrices available for children is only 449.5 ± 26.1 ppm. Only very few toothpastes with the recommended concentration of fluoride is available in the Indian market, for children below the age of 6 years. Aims: To compare and evaluate the remineralization potential and antimicrobial efficacy of toothpaste with highest amount of fluoride, available in India for children below 6years of age, with non-fluoridated remineralizing children’s toothpaste. To assess the Total Soluble Fluoride (TSF) concentration of fluoridated toothpastes. Methodology: 95 extracted or naturally exfoliated human primary teeth were decoronated and sectioned mesiodistally. Specimens were demineralized and randomly divided into six groups (3 fluoridated and 3 non-fluoridated remineralising toothpaste groups): group 1: Omnident®- 917ppm, group 2: AquaFresh®™ little teeth- 1450ppm, group 3: Colgate kids®- 1000ppm, group 4: Enafix® (Ca. orthophosphate + Ca. Sucrose phosphate), group 5: Aclaim® (Nano HAP) and group 6: Shy-NM® (Novamin). The samples were placed in the demineralizing solution for 48h and subjected to Vickers hardness test (VHT) and SEM to obtain the baseline values. Then the samples were remineralized using the respective toothpastes and were subjected to SEM and VHT at 21days, 3months and 6months intervals. Antimicrobial activity of all the dentifrices was determined by agar well diffusion method and selective ion electrode analysis was used to evaluate the Total Fluoride (TF) and Total Soluble Fluoride (TSF) concentrations among the three fluoridated toothpastes. The data obtained were statistically analysed. Results: SEM images showed mineral deposition in all the groups obliterating the defects caused due to demineralization at various intervals. Remineralising agents showed significantly higher remineralisation especially SHY-NM when compared to Fluoridated toothpastes. However they did not show any antimicrobial efficacy. Fluoridated toothpastes showed antimicrobial efficacy against S. mutans and the highest antimicrobial efficacy was seen with Aquafresh followed by Omnident and Colgate Kids which was directly proportional to the TSF concentration of the toothpastes. Conclusions: Highest remineralisation of artificial enamel lesions was shown by non- fluoridated remineralising toothpaste, however, the fluoridated dentifrices displayed both antimicrobial and remineralising properties. The Total Soluble Fluoride (TSF) concentration of fluoride toothpastes were generally slightly less than the Total Fluoride (TF) concentration (TSF/F (%): 97%- AquaFresh®TM, 61.1%-Colgate kids® and 96.5% in Omnident®), which can be correlated to the dose-dependant antimicrobial efficacy as well as remineralisation of the fluoridated toothpastes. Keywords: remineralization potential, antimicrobial efficacy, toothpaste with highest amount of fluoride, dental caries.

Active Surveillance of Root Caries in Vivo with CP-OCT.

The active surveillance of root caries lesions to monitor potential remineralization or decay progression is challenging for the clinician, due to unreliable diagnostic information. The conventional visual and tactile methods for assessing the lesion activity are not reliable, and the clinician is often unable to determine if the lesion is progressing or has been arrested. An important marker of an arrested lesion is a highly mineralized transparent surface zone (TSL) that forms when the mineral is deposited in the outer layer of the lesion. The purpose of this study was to determine if cross-polarization optical coherence tomography (CP-OCT) could be used to detect changes in the lesion severity and activity during active monitoring. In total, 18 subjects with 22 suspected active root caries lesions were evaluated using CP-OCT at the baseline, 3 months, and 6 months. All subjects were instructed to use a high fluoride dentifrice at the baseline. The results showed that CP-OCT was able to discriminate the active from the arrested lesions by identifying the presence of a TSL on arrested lesions. The results also indicated that the mean TSL thickness increased significantly (p < 0.05) for the nine lesion areas. In addition, CP-OCT was able to show the progression of demineralization, erosion, and changes in gingival contours in scanned areas. CP-OCT was valuable for monitoring the activity and severity of root caries lesions in vivo. CP-OCT can be used to assess the activity of root caries lesions at a single time point by detecting the presence of a TSL at the lesion surface indicative of the lesion arrest.

Ecological influence by colonization of fluoride-resistant Streptococcus mutans in oral biofilm.

Dental caries is one of the oldest and most common infections in humans. Improved oral hygiene practices and the presence of fluoride in dentifrices and mouth rinses have greatly reduced the prevalence of dental caries. However, increased fluoride resistance in microbial communities is concerning. Here, we studied the effect of fluoride-resistant Streptococcus mutans (S. mutans) on oral microbial ecology and compare it with wild-type S. mutans in vitro. Biofilm was evaluated for its polysaccharide content, scanning electron microscopy (SEM) imaging, acid-producing ability, and related lactic dehydrogenase (LDH), arginine deiminase (ADS), and urease enzymatic activity determination. Fluorescence in situ hybridization (FISH) and quantitative real-time polymerase chain reaction (qRT-PCR) were used to evaluate the S. mutans ratio within the biofilm. It was followed by 16S rRNA sequencing to define the oral microbial community. Fluoride-resistant S. mutans produced increased polysaccharides in presence of NaF (P < 0.05). The enzymatic activities related to both acid and base generation were less affected by the fluoride. In presence of 275 ppm NaF, the pH in the fluoride-resistant strain sample was lower than the wild type. We observed that with the biofilm development and accumulative fluoride concentration, the fluoride-resistant strain had positive relationships with other bacteria within the oral microbial community, which enhanced its colonization and survival. Compared to the wild type, fluoride-resistant strain significantly increased the diversity and difference of oral microbial community at the initial stage of biofilm formation (4 and 24 h) and at a low fluoride environment (0 and 275 ppm NaF) (P < 0.05). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that fluoride-resistant strain enhanced the metabolic pathways and glucose transfer. Fluoride-resistant S. mutans affected the microecological balance of oral biofilm and its cariogenic properties in vitro, indicating its negative impact on fluoride's caries prevention effect.

Situational diagnosis of policies in Latin American and Caribbean countries for the use of fluoride and reduction of sugar consumption.

Policy evaluation and guidance on fluoride use and sugar consumption in Latin American and Caribbean countries (LACC) may provide a scientific evidence basis for policymakers, dental professionals, civil society organizations and individuals committed to improving public oral health. A cross-sectional study was conducted to evaluate the extent of implementation of policies/guidelines on fluoride use, and sugar consumption in LACC. The study had two stages. First a questionnaire covering four major areas was developed: fluoridation of public water supplies; salt fluoridation; fluoride dentifrices, and sugar consumption. Then, the questionnaire was applied to collect data among representative participants in public oral health from LACC. Ninety-six participants from 18 LACC answered the questionnaire. One-hundred seventy documents were attached, and 285 links of websites were provided by the respondents. Implementation of policies and guidelines on water and table salt fluoridation and processed and ultra-processed food consumption were found in most countries, with some issues in the consensus and coverage. Thus, differences were identified in the extent of implementation of public oral health strategies on sugar consumption and fluoridation among the countries. There is no consensus on the policies in LACC to reduce sugar consumption and for the use of fluoride. A few policies and guidelines were applied in isolated countries, with a variety of strategies and standards. For future actions, it will be important to encourage the development of strategies and public policies within countries, and to evaluate the effectiveness of existing policies in reducing dental caries and in improving oral health in LACC.

Fluoride concentration in dentifrices marketed In Argentina.

The objective of this study was to determine the different forms of fluoride available in dentifrices commercialized in Argentina. Thirty three different brands of fluoridated dentifrices were purchased in different cities of Argentina. The total fluoride (TF), total soluble fluoride (TSF) and ionic fluoride (IF) of two samples of each brand were analysed (μg F/ml) by ion-specific fluorine electrodes. A total of 67% of dentifrices showed fluoride content that compromises anti-caries activity. A total of 54.5% was formulated with sodium fluoride (NaF), 36.3% formulated with sodium monofluorophosphate (MFP), 3% NaF associated with MFP and 9% formulated with stannous fluoride (SnF2). The TF found was less than the amount declared by the manufacturer in all the brands analysed. The concentration of TF found in the fluoride dentifrices ranged from 590.3 to 1381.4μg/ml F, TSF ranged from 343.1 to 1258.3μg/ml F, and IF ranged from 13.1 to 913μg/ml F. The concentrations of TF were below the reported values. TSF and IF were lower than the total fluoride concentration. Most of the samples studied have TSF concentration that compromises anti-caries effect. Additional studies with a larger number of samples are suggested to obtain more data.

Evaluation of remineralizing potential of hydroxyapatite, phosphopeptide-amorphous calcium phospahate and fluoride dentifrices using SEM/EDX analysis: A randomized controlled in-vitro study

The aim of this study is to evaluate remineralization efficiency of hydroxyapatite (HA) containing dentifrices comparing to phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride (F) containing dentifrices using scanning electron microscope (SEM) and energy dispersive spectrometry (SEM/EDX). 3 × 3 mm 2 areas on the buccal surfaces of extracted primary second molars were divided vertically into 3 sections as demineralization , control and remineralization surfaces. Control surfaces (1/3 middle) coated without any application. 1/3 left and right surfaces were subjected to demineralization solution. After this application 1/3 right surfaces were coated as demineralization surfaces. Then teeth were randomly separated in 3 groups (n = 10): group HA (Natural toothpaste, Splat®, Russia); Group CPP-ACP (MI Paste ONE®, GC, U.S.); group F (Oral-B® Kids, Oral-B, Germany). 1/3 right surfaces were brushed with dentifrices twice a day for one week with pH cycle. Coatings were removed. Surface evaluations were conducted by SEM and EDX. Mann Whitney U and Kruskal Wallis tests were used for statistical analysis. Both atomic (at%) and weight (wt%) percentages of P values were statistically significant for F group in remineralization surfaces comparing to demineralization surfaces. Ca at%, P at% and P wt% values were statistically significant for CPP-ACP group in remineralization surfaces. P at% and P wt% values for F group were higher in remineralization surfaces compared to all. Remineralization was observed in all groups. Most homogeneous surface features were seen in group CPP-ACP. • Dentifrice containing hydroxyapatite showed favourable results comparable to fluoride containing dentifrice. • CPP-ACP containing dentifrice has not been evaluated before in any study. Most of the studies has assessed CPP-ACP varnishes. • It is important to know the efficacy of these materials in toothpaste formula. As pediatric dentists, we should be able to recommend toothpaste containing an effective remineralization agent for groups where fluoride supplementation or fluoride use is not preferred. • CPP-ACP dentifrice showed promising results compared to fluoride. • EDX analysis provides the opportunity to work without damaging the sample in remineralization studies. Primary teeth are valuable samples for studies.

Functional changes in the oral microbiome after use of fluoride and arginine containing dentifrices: a metagenomic and metatranscriptomic study

BackgroundTooth decay is one of the most prevalent diseases worldwide, and efficient tooth brushing with a fluoride-containing dentifrice is considered fundamental to caries prevention. Fluoride-containing dentifrices have been extensively studied in relation to enamel resistance to demineralization. Arginine (Arg) has also been proposed as a promising prebiotic to promote pH buffering through ammonia production. Here, we present the first metagenomic (DNA sequencing of the whole microbial community) and metatranscriptomic (RNAseq of the same community) analyses of human dental plaque to evaluate the effect of brushing with fluoride (Fl) and a Fl+Arg containing dentifrices on oral microbial composition and activity. Fifty-three patients were enrolled in a longitudinal clinical intervention study with two arms, including 26 caries-active and 27 caries-free adults. After a minimum 1-week washout period, dental plaque samples were collected at this post-washout baseline, 3 months after the use of a 1450-ppm fluoride dentifrice, and after 6 months of using a 1450-ppm fluoride with 1.5% arginine dentifrice.ResultsThere was a shift in both the composition and activity of the plaque microbiome after 3 months of brushing with the fluoride-containing toothpaste compared to the samples collected at the 1-week post-washout period, both for caries-active and caries-free sites. Although several caries-associated bacteria were reduced, there was also an increase in several health- and periodontitis-associated bacteria. Over 400 genes changed proportion in the metagenome, and between 180 and 300 genes changed their expression level depending on whether caries-free or caries-active sites were analyzed. The metagenome and metatranscriptome also changed after the subjects brushed with the Fl+Arg dentifrice. There was a further decrease of both caries- and periodontitis-associated organisms. In both caries-free and caries-active sites, a decrease of genes from the arginine biosynthesis pathway was also observed, in addition to an increase in the expression of genes associated with the arginine deiminase pathway, which catabolizes arginine into ammonia, thereby buffering acidic pH. Bacterial richness and diversity were not affected by either of the two treatments in the two arms of the study.ConclusionsOur data demonstrate that long-term use of both assayed dentifrices changes the bacterial composition and functional profiles of human dental plaque towards a healthier microbial community, both in caries-free and caries-active sites. This observation was especially apparent for the Fl+Arg dentifrice. Thus, we conclude that the preventive benefits of tooth brushing go beyond the physical removal of dental plaque and that the active ingredients formulated within dentifrices have a positive effect not only on enamel chemistry but also on the metabolism of oral microbial populations.-kQdKShqK4FxgvJNrxZQaCVideo

Dental floss as an adjuvant of the toothbrush helps gingival health.

Design and aim This randomised controlled clinical trial assessed the efficacy of using dental floss in addition to toothbrushing in reducing gingival and plaque indices.Intervention The participants received oral hygiene instruction and supervised personal and professional plaque removal every week for two months. The research team instructed the participants to perform toothbrushing twice daily - groups 'toothbrushing and flossing' (TB + DF) and 'toothbrushing alone' - and use dental floss once daily - group TB + DF. Gingival index (GI) and plaque idex (PI) were evaluated at baseline and after one and two months.Case selection The authors invited the participants via the Federal University of Santa Maria screening service, social media and public posters. The participants were 18 years and older, without interproximal clinical attachment loss, with papillae completely filling the interproximal space and with at least 24 teeth remaining. The participants were included if they had a gingival index score of two at more than 15% of the interproximal sites. The authors excluded dental students, patients with xerostomia or diabetes, pregnant women, smokers, patients with orthodontic appliances and restraints, patients requiring antimicrobial prophylaxis, patients having used antibiotics/anti-inflammatory agents within the last three months and those with psychomotor disorders.Experimental groups and data analysis The authors used a block randomisation sequence with block sizes of ten. Randomisation confidentiality was maintained using serially sealed, numbered, opaque envelopes. At baseline, all the participants received oral prophylaxis and their GI, probing depth (PD), clinical attachment loss (CAL) and bleeding on probing (BOP) were assessed. A clinical staff member disclosed the experimental group assignments, distributed scheduled oral hygiene materials and conducted assigned study interventions. Each participant received a soft toothbrush (Colgate Twister Compact Head, New York, NY, USA) and fluoride dentifrice (Colgate Triple Action, 90 g, New York, NY, USA). Patients were instructed to brush their teeth twice daily. Subjects from the TB + DF group also received dental floss and were asked to use it once daily (Colgate, Tarpaulin, New York, NY, USA). The research team supervised the oral hygiene practices at day zero and recalled the patients to provide them with personalised oral hygiene instruction and dental plaque removal. Individual patients were considered as units of analysis. PI, GI, CAL, PD, gingival bleeding (GI = 2), BOP and percentage sites with different GI scores were presented as means, standard deviations and standard errors. The authors used multiple imputations to replace missing data and mixed linear models to analyse and compare the experimental groups.Results In total, 75 participants were randomised. Both groups showed a significant reduction in gingival inflammation (GI = 2) in the first month, albeit no change was seen in the second month. Adding flossing to toothbrushing significantly reduced the GI = 2 by the end of the first month (37.7 to 15.9%), maintaining optimised overall oral health through day 60 (15.1%) compared with toothbrushing alone. No significant differences were noted between the two groups from the first to the second month. Interproximal dental plaque was reduced for both groups from baseline to day 30 with no marked change from then to day 60. Plaque reduction was similar in both groups.Conclusions Toothbrushing reduces interdental plaque build-up and halts its progression while improving gingival health in adults with no interdental attachment loss. Dental flossing as an adjuvant to toothbrushing also enhances these benefits.

Effect of a Stannous Fluoride Dentifrice on Biofilm Composition, Gene Expression and Biomechanical Properties.

An in situ study was conducted to examine the mode of action of a 0.454% stannous fluoride (SnF2)-containing dentifrice in controlling the composition and properties of oral biofilm. Thirteen generally healthy individuals participated in the study. Each participant wore an intra-oral appliance over a 48-h period to measure differences in the resulting biofilm’s architecture, mechanical properties, and bacterial composition after using two different toothpaste products. In addition, metatranscriptomics analysis of supragingival plaque was conducted to identify the gene pathways influenced. The thickness and volume of the microcolonies formed when brushing with the SnF2 dentifrice were dramatically reduced compared to the control 0.76% sodium monofluorophosphate (MFP)-containing toothpaste. Similarly, the biophysical and nanomechanical properties measured by atomic force microscopy (AFM) demonstrated a significant reduction in biofilm adhesive properties. Metatranscriptomic analysis identified pathways associated with biofilm formation, cell adhesion, quorum sensing, and N-glycosylation that are significantly downregulated with SnF2. This study provides a clinically relevant snapshot of how the use of a stabilized, SnF2 toothpaste formulation can change the spatial organization, nanomechanical, and gene expression properties of bacterial communities.

The Use of Fluorides in Public Health: 65 Years of History and Challenges from Brazil.

Untreated dental caries is the most common disease globally and fluoride use at the population level is crucial for its control. To investigate the economic and political conditions under which the trajectory of population-based fluoride use has occurred is key for a more comprehensive view on its current and future challenges. The objective was to give a brief history and summarize the information on the use of fluorides at the population level in Brazil from 1952 to 2017 and to point to current and future challenges. A critical overview was undertaken based on country-level analyses comprising political and economic conditions. The analytical approach adopted a set of premises applicable to the study of capitalist democracies. Fluoride methods of systemic and topic use began to be employed in Brazilian public health programs in the 1950s and in a combined way from 1974. Differences in political and economic contexts were highlighted for four periods: the first interventions from 1952 to 1974, when the fluoridated water law was approved; the expansion after 1974 until 1988, when a new constitution was enacted; the following time until 2010; and the final period. From the 1980s to 2008, water fluoridation coverage increased progressively, consolidating as a major strategy of systemic use in spite of inequalities among territories. Activities aimed to promote access to topical fluoride use increased and maintained stability until 2014, when they dropped sharply. Regulation of fluoride dentifrice’s quality remained insufficient. It was hypothesized that the strengthening of conservative liberalism and the increase in fiscal austerity observed in recent years might produce serious constraints on public investment and limit access to fluorides. To reduce inequities and promote benefits for all, including the most vulnerable groups, policies based on egalitarian and social justice theoretical perspectives are needed more than ever.

Assessment of Oral Health-Related Quality of Life among Kutia Kandha Tribes of Odisha.

Aim and Objectives:Among the 75 listed particularly vulnerable tribal groups (PVTG), the highest number is found in Odisha. They do not have proper access to oral health-care services and at-risk to various oral conditions and lesions. Hence, the purpose of the study was to assess the oral health quality of life and its association with different factors of the Kutia Kandha tribal population.Materials and Methodology:A cross-sectional study was channeled among 600 Kutia Kandha tribe of Odisha. The oral health impact profile (OHIP-14) questionnaire was used to check the oral health-related quality of life. Number and percentages were derived using Microsoft Excel and for inferential statistics, a model was developed using multivariable logistic regression using STATA software. P was set at 0.05, which was considered to be statistically significant.Results:Total sample composed of 330 men and 270 women with a mean age of 40.62 ± 16.29 years. Smoking was seen among 19.8% of tribal people had smoking habit and 72.33% (n = 434) of the study group consumed smokeless tobacco. Only a few used fluoridated (3.8%) dentifrice. The mean OHIP score of the tribe was 30.67 ± 4.514 and about 65% of participants reported poor oral health quality of life scores.Conclusion:The oral health quality of life of the tribe is poor and the prevalence of tobacco among the target population because of the unavailability of dental services. Proper health education and motivation can be acknowledged to this group are required to improve their oral health.

COMPARATIVE EVALUATION OF FLUORIDE RECHARGE OF FLUORIDE RELEASING DENTAL MATERIALS BY FLUORIDATED DENTIFRICE : AN IN VITRO STUDY

Introduction: Fluoride releasing dental materials releases maximum fluoride within twenty four hours which reduces thereafter.If we could recharge fluoride on the daily basis,the caries preventive effect from fluoride releasing dental materials can be prolonged. Aim and Objectives: The present study is undertaken to compare and evaluate fluoride recharge of fluoride releasing dental materials by fluoridated dentifrice. Materials and Method: Materials used in this study are GC Fuji Type IX,Fuji Plus,Zirconomer Improved,Beautifil II LS. All restorative materials were mixed according to manufacturers instruction and standardized test pellets were made using autoclaved instruments in sterile plastic moulds. These test pellets were divided in various groups and brushed with fluoridated dentifrice accordingly . Results were evaluated statistically using Post Hoc test and ANOVA and correlation coefficient for which regression lines were drawn. Result: The mean fluoride release and recharge was maximum in Zirconomer Improved, followed by GC Fuji Plus ,then by GC Fuji Type IX and least by Beautifil II LS. Conclusion:Daily recharge of fluoride releasing dental materials with fluoridated dentifrices is recommended to increase the cariostatic effect.

Virucidal Activity of Over-the-Counter Oral Care Products Against SARS-CoV-2.

The oral cavity is an important entry point for SARS-CoV-2 infection. This study tested whether four commercially available mouthrinses and dentifrices have in vitro virucidal activity against SARS-CoV-2 (=4 log10 reduction in viral titer). One part of stock SARS-CoV-2 virus plus one part 0.3 g/l bovine serum albumin were mixed with eight parts of test product solution. After 30 s for the rinses, or 60 s for the dentifrices, the mixture was quenched in an appropriate neutralizer, serially diluted, and inoculated onto Vero E6 cells to determine viral titer. Triplicate runs were performed for each test condition with appropriate controls for test product cytotoxicity, viral interference, and neutralizer effectiveness. Test products included: 1.5% hydrogen peroxide (H2O2) rinse; 0.07% cetylpyridinium chloride (CPC) rinse; 0.454% stannous fluoride (SnF2) dentifrice A; and 0.454% SnF2 dentifrice B. ?The 1.5% H2O2 rinse, 0.07% CPC rinse, SnF2 dentifrice A, and SnF2 dentifrice B all produced > 4 log10 reduction in SARS-CoV-2 titer. All four test products displayed potent virucidal activity in vitro. Clinical studies are warranted to determine what role, if any, these oral care products might play in preventing transmission of SARS-CoV-2 or in the management of patients currently diagnosed with COVID-19 illness.

A Systematic Review and Meta-Analysis of the Relationship Between the Severity of Dental Fluorosis and Fluoride Biomarkers in Endemic Areas.

The intake of high concentrations of fluoride, mainly through drinking water, diet and fluoridated dentifrices, produces fluorosis, which in its early stages is manifested as dental fluorosis (DF). To recognize exposure to fluoride in endemic areas and to evaluate the risk of developing health impairment, the WHO has established several biomarkers that are used to determine systemic fluorine (F-) exposure. Thus, the aim of this study was to conduct a systematic review and meta-analysis of the relationship between the severity of DF and fluoride biomarkers in endemic areas. The protocol of this study was previously registered as CRD42021244974. A digital search was carried out in PubMed/Medline, SpringerLink, Scopus, Cochrane and Google Scholar by employing the keywords "urine", "nails", "hair", "plasma", "saliva" and "dental fluorosis" for the original studies with content associated with F- for the biomarkers and DF. The mean difference was established as the effect measure for the meta-analysis. Seven studies fulfilled the eligibility criteria, among which five assessed urine and two employed nails as fluoride biomarkers. A positive significant difference was found between the biomarkers and the severity of DF (0.27, p < 0.001) and individually for each biomarker (urine: 0.14, p = 0.001; nails: 0.88, p < 0.05). The F- concentration in urine and nails is correlated with the severity of DF, with the most evident differences between healthy individuals and those with mild severity. Both biomarkers are adequate to assess this relationship in endemic areas of fluoride and DF.

The Influence of Preschool Children's Mothers on the Knowledge, Attitude and Practice of Using Fluoride Toothpaste

Dental caries in Sri Lanka appears to be a significant problem, especially among preschool children. The effect of caries prevention in the primary teeth of preschool children through the use of fluoridated dentifrices and prevention programs has not been widely addressed. A descriptive cross sectional study was carried out by recruiting 635 mothers and their children in Panadura, Sri Lanka. Multi-stage cluster sampling technique was used and preschools considered as clusters to select the sample. A pre-tested interviewer-administered questionnaire was used to assess the knowledge, attitudes and practices. Only 66% (n=418) of mothers had fair knowledge and 2.8% (n=18) had good knowledge and the mean of the total knowledge score was 3.2 (SD ± 1.7) out of 7. Seventy four point six (n=472) had favourable attitude towards fluoridate toothpaste.83.1% of mothers and 76.6% children were using fluoridated toothpaste. 83.6% of mothers and 83.6% of children brushed their teeth twice or more than twice daily. A pea sized amount or more of toothpaste was used by 79.2% of children and 52.3% of children’s teeth were cleaned by a parent or a grandparent.81.8% of children had started using toothpaste at the age of one year or before. The results of the present study highlight the importance of giving instructions on caring for baby’s teeth during pregnancy including the selection of toothpaste for the child, time of initiation of using toothpaste, amount of toothpaste that should be used for the particular age group.

Changes in enamel surface after use of nanoencapsulated fluoride for dental caries remineralization: an in vitro study

Aim: To evaluate through an in vitro study surface changes on decayed enamel after the use of fluoridated dentifrices involving nanoencapsulated technology (NanoF). Methods: Forty blocks of human enamel were distributed among four groups (n = 10): 50% NanoF + 50% NaF (50% nF), 100% NanoF (100% nF), 100% NaF as the positive control (PC) and without fluoride as the negative control (NC). The specimens were subjected to a pH cycling model for 7 days. Surface microhardness (SMH), linear surface roughness (Ra) and area surface roughness (Sa) were measured before and after the carious lesion formation and at the end of the treatment. Percentages of surface microhardness recovery (%SMHR), altered Ra (%RaC) and altered Sa (%SaC) were calculated. The data were analyzed using the ANOVA test, repeated-measures ANOVA and Pearson's correlation test (p&lt;0.05). Results: No significant differences among groups were found for Ra, Sa, %RaC or %SaC (p&gt;0.05). Ra and Sa increased after treatment. A significant correlation was found between Ra and Sa. The PC dentifrice had the highest %SMHR, followed by 100% nF (p&lt;0.05). In contrast, no surface remineralization was found in the 50% nF and NC groups. Conclusion: Enamel surface changes were found after the remineralizing treatment with 100% nF group. The majority of dentifrices with fluoride provided an increase in the roughness and surface microhardness. Nanotechnology is an innovative, promising method for the controlled release of fluoride and the remineralization of teeth with dental caries.

Application of Near-infrared Light Transillumination in Restorative Dentistry: A Review

This paper presents the various applications of near-infrared light transillumination (NILT) in dentistry. Untreated dental caries is considered the most prevalent health condition affecting both children and adults worldwide. Increased awareness and a paradigm shift toward utilization of minimally invasive treatment procedures and nonionizing radiation led to the discovery of newer techniques for screening and early diagnosis of demineralized lesions. Demineralized lesions detected early can be treated with minimally invasive treatment procedures such as the usage of fluoridated dentifrice to encourage remineralization and resin infiltration to arrest caries progression. NILT procedure involves noninvasive, nonionizing radiation and helps in the identification of early demineralized lesions using light transillumination. At near-infrared wavelengths, the enamel appears translucent and helps in visualizing and detecting demineralized lesions when long-wave light transilluminated against the tooth surface. The wavelength in the range of 1310 nm is considered best for the transillumination of lesions. This technique has been proven to be successful in the detection of carious and demineralized lesions. NILT can be used as a screening tool for the early detection of demineralized lesions and can be considered as an adjunct to bitewing radiographs. It can be advantageous in screening pregnant, growing adolescent patients and in cases where multiple follow-ups are needed and ionizing radiation must be avoided. Keywords: Dental caries, Early diagnosis, Ionizing radiation, Minimally invasive, Near-infrared, Occlusal caries, Transillumination.