Fish Poisoning Research Articles

Unlocking Wild Edible Fruits of Indo-Burma Biodiversity Hot Spot, Arunachal Pradesh, India, to Support Food Security and Sustainable Rural Livelihood

Forests inhabit a large number of trees, shrubs, climbers, non-timber forest products and a number of wild edible fruits. These wild edible fruits are an indispensable constituent of human life and rich in vitamins, minerals, fibres, antioxidants, alkaloids, and phenolics. Many of them are good source of medicines. The present study was undertaken to document wild edible fruits of Arunachal Pradesh, India, and their socioeconomic importance in the sustainable rural economy. The study revealed that altogether 50 wild edible fruits belonging to 31 families are used against a number of ailments. They have a number of uses in the socioeconomic life of rural people such as constructional timbers, firewood, charcoal, fodder, fish poisoning, dyes, oils, etc. Although these fruits play an important role in the life of tribal people, these valuable species have been vanishing at a very fast rate. Therefore, promoting their sustainable use and increasing their abundance in different landscapes through the agroforestry system must immediately be undertaken. The claimed therapeutic values are to be critically studied and dynamic conservation of associated indigenous knowledge among local tribal communities should be carried out through policy interventions, implementation of farmer’s rights, and generation of awareness among the local people. Policy makers should immediately undertake strategic plans for both in situ and ex situ conservation of wild edible fruits that have ethno-medicinal values. In addition, the orally transmitted therapeutic values of wild edible fruits should be urgently documented to safeguard these valuable species from further extinction.

Histamine Content in Selected Production Stages of Fish Products.

Histamine intoxication, known as scombroid fish poisoning, is caused by the consumption of foods with high levels of histamine. This biogenic amine is formed as a result of histidine decarboxylation by bacterial decarboxylases present in food, including fish and fish products. The aim of this study was to investigate the content of histamine at different production stages of canned, marinated and smoked fish. Raw fish, semi-finished fish products, and the final products of the same production batches were collected between 2019 and 2022 from different fish production facilities in Poland. A total of 133 raw fish samples and 76 smoked fish, 54 brined fish, 39 canned fish and 18 marinated fish final products were analysed using high performance liquid chromatography with a diode array detector. Histamine was identified in 55 (17.2%) out of 320 tested samples, including 8 samples of raw fish with a histamine level above 100 mg/kg. However, no samples of fish products had histamine content above the European Union Commission limit. The obtained results show that fish products on the Polish market are generally safe for consumers in regard to histamine intoxication risk.

Preventive effects of Tephrosia lupinifolia DC. on haematological alterations induced by cadmium in Sprague-Dawley rats

Introduction: Cadmium is known to be one of the most noxious environmental and industrial pollutant. Cigarette smoke and food products are the major sources of cadmium exposure in non-occupationally exposed people. Tephrosia lupinifolia DC. (Fabaceae) is commonly known as Fish poison. It has usage in treatment of diarrhea, stomachache, urinary disorders, asthma and rheumatism. Objectives: This investigation was aimed to examine the protective potentials of Tephrosia lupinifolia DC. against cadmium-induced changes in haematological parameters of Sprague-Dawley rats. Methodology: In present study, male rats (n=30) were taken and equally divided into five groups. Rats were orally administrated with relevant doses of methanolic extract of Tephrosia lupinifolia (25, 50 mg/kg bw) for 7 days and then CdCl2 (4 mg/kg) was intravenously injected to produce acute toxicity. At the end of experiment, rats were dissected to collect blood for haematological analysis. Results: Cadmium treatment caused significant decline of RBCs (P=0.002), PCV and Hb concentration (P=0.0001) as compared to control. It also elevates the WBC count (P<0.001), concentration of lymphocytes, neutrophil percentages, MCV, MCHC and MCH values (P=0.05). The changes induced by cadmium in different haematological parameters were improved in the rats treated with Tephrosia lupinifolia. Conclusion: The outcomes of this study recommended that methanolic extract of Tephrosia lupinifolia (TLME) ameliorates the harmful effects of CdCl2 on blood parameters which can be credited to the presence of different phytochemical constituents in it.

日本沿岸産イシガキダイのシガトキシン分析

Ciguatera fish poisoning (CFP) is recognized as the most frequent seafood poisoning due to the consumption of fish containing the principal toxins, ciguatoxins (CTXs). In Japan, CFP events have been reported annually from Okinawa and Amami Islands, locating subtropical regions. In addition, there have been reported several outbreaks due to consumption of the fish caught from the Pacific coast of the Mainland and they were often caused by the matured spotted knifejaw, Oplegnathus punctatus. As part of our research on CFP in Japan, we investigated CTXs analysis by LC-MS/MS on 176 individuals of O. punctatus (weight: 100-6,350 g, standard length: 13-60 cm) from the coast of the Mainland (Honshu, Shikoku, and Kyushu), Amami, Okinawa, and Ogasawara (Bonin) Islands. CTXs were detected from only two specimens collected from Okinawa. Total CTXs levels of the two specimens were at 0.014 and 0.040 μg/kg, respectively, exceeding FDA guidance level at 0.01 μg CTX1B equivalent/kg. However, they might be little risk of CFP because consuming over 1.5 kg of flesh is needed to develop intoxication. The toxins consisted of CTX1B analogs including CTX1B, 52-epi-54-deoxyCTX1B, CTX4A, and CTX4B, and no CTX3C analogs, supporting the finding that ciguatoxic fishes in Okinawan Waters containing only CTX1B analogs.

Toxicity and underlying mechanism of the toxic dinoflagellate Gambierdiscus caribaeus to the fish Oryzias melastigma

Gambierdiscus spp. is mainly responsible for the ciguatera fish poisoning (CFP) around the world. The gambiertoxin produced by Gambierdiscus can be passed through the food chain to form ciguatoxins (CTXs) that cause ciguatoxins poisoning. However, the toxic effects of Gambierdiscus on fish through the food chain and related mechanism remains unclear. In this study, the toxicity of Gambierdiscus caribaeus on the marine medaka (Oryzias melastigma) was investigated, where the simulated food chain toxic algae-food organism-fish (G. caribaeus-Artemia metanauplii-O. melastigma) was set. The results showed that direct or indirect exposure through the food chain of G. caribaeus could affect the swimming behaviour of O. melastigma, manifested as decreased swimming performance and spontaneous abnormal swimming behaviours. Histological observation showed that direct or indirect exposure of G. caribaeus caused different degrees of pathological damage to the gills, intestine and liver tissues of O. melastigma. Transcriptome sequencing and RT-qPCR demonstrated that G. caribaeus exposure could trigger a series of physiological and biochemical responses, mainly reflected in energy metabolism, reproductive system, neural activity, immune stress and drug metabolism in marine medaka. Our finding may provide novel insight into the toxicity of Gambierdiscus on fish.

2-Methoxyjuglone, a Promising Bioactive Compound for Pharmaceutical and Agricultural Purposes: A Review.

2-Methoxyjuglone, a member of the 1,4-naphthoquinone family, was first obtained through semi-synthesis based on 2-hydroxyjuglone as the precursor in 1966. It has been isolated and identified from different plant species of the Juglandaceae, Sterculiaceae, and Proteaceae families. 2-Methoxyjuglone has been demonstrated to possess a wide range of biological activities, including antitumor, antifungal, and antibacterial activities; in addition, it has been shown to poison fish and inhibit seed germination. These properties make 2-methoxyjuglone a promising bioactive compound for pharmaceutical and agricultural purposes. This review article provides an overview of the current research progress on 2-methoxyjuglone for the first time, with a primary focus on the plant sources, extraction, identification, synthesis, and biological activities associated with this compound for further development.

Comparative morphological and anatomical traits of Adenia lobata (Jacq) Engl. and Adenia cissampeloides (Planch. ex Benth.) Harms. (Passifloraceae)

Comparative morph-anatomical studies of leaf, stem, and tendril were carried out on two Adenia species to enhance the delimitation of the species. The Adenia species have alternate phyllotaxy, simple leaf, glands at the base of their petioles, hypostomatic, similar stomata types, solitary crystal, similar vascular bundle type, tanniferous cells, and dorsiventral leaves. Both species have tetracytic and anomocytic stomata in addition A. lobata had paracytic, and anisocytic stomata. Mucilaginous cells or cavities occurred in the petiole, leaf lamina, midrib, stem, and the tendril of A. cissampeloides but were only observed in the tendril and upper epidermis of A. lobata. The mucilaginous and tanniferous cells were more abundant in A. cissampeloides compared to A. lobata and could account for the reason why A. cissampeloides is used as fish poison. The sclereids in A. cissampeloides are surrounded by lignified cells while the ones in A. lobata do not have such feature. Also, xylem cells in the A. cissampeloides tendril are thicker compared to A. lobata. This could be attributed to the fact that the fruits in A. cissampeloides are directly attached to the tendril. The outline and number of vascular bundles in the midrib and petiole differed among the two species. In A. lobata, the ridge averaged 130 µm thick and 73.76 µm in A. cissampeloides. The analysed characteristics are valuable in the delimitation of these Adenia species.

Traditional Aucan knowledge on fish and plants eaten by fish along the Tapanahoni River, Suriname

Suriname’s freshwater systems are home to a large diversity of (endemic) fish species, and communities of Suriname’s interior strongly depend on this diversity for their nutrient intake. However, studies on traditional knowledge of the country’s freshwater fish and fish-plant interactions are scarce. Here, we present our findings of a pilot study in the Aucan Maroon community of Diitabiki (Tapanahoni river). We report the species of freshwater fish caught for food, their corresponding Aucan names and plants eaten by fish and/or used for fishing by the local population. We held semi-structured interviews and performed participant observation with fishers and forest guides in August and September 2021. We recorded 14 fish species, of which 12 were identified to species level, and recorded 16 Aucan fish names, of which nine were previously undocumented. Furthermore, we reported 11 plant species that were used for fishing and one fish poison (Tephrosia sinapou (Buc’hoz) A.Chev.). Suriname’s riverine ecosystems are threatened by gold mining activities that endanger the health of local communities, as well as the fish populations and riverine forests on which both people and fish depend. Local knowledge on fish, their feeding behavior and the flooded forests is essential for the conservation of this important Amazonian ecosystem, for the development of sustainable management plans and health education programs on mercury levels in consumption fish.

4-tert-butylphenol triggers common carp hepatocytes ferroptosis via oxidative stress, iron overload, SLC7A11/GSH/GPX4 axis, and ATF4/HSPA5/GPX4 axis

4-tert-butylphenol (4-tBP) is a toxic environmental pollutant with moderate bioaccumulation, environmental persistence, and long-term toxicity. Its toxicity to aquatic organisms has become an issue of concern. However, the molecular mechanism of 4-tBP toxicity to aquatic organisms remained unclear. Liver is a target organ for environmental pollutants. Here, we established 4-tBP-exposed toxicity model in vivo and primary hepatocyte model in vitro in common carp (Cyprinus carpio L.). We found increased hepatic-somatic index (HSI) and abnormal serum biochemical indexes (ALT, AST, and LDH) after 4-tBP exposure, indicating liver damage. We further revealed that 4-tBP damaged the structural integrity of the livers with typical features of ferroptosis. Based on toxicogenomics analysis, we found ferroptosis is likely to be involved in the mechanism of 4-tBP-induced liver damage. Moreover, our in vivo and in vitro experiment provided evidences that 4-tBP-exposure led to excess oxidative stress, iron overload, decreased MMP, and abnormal expression of ferroptosis-related factors. Interestingly, ferrostatin-1 (Fer-1, a ferroptosis inhibitor) pretreatment alleviated above changes. In summary, we demonstrated that 4-tBP triggered hepatocytes ferroptosis via oxidative stress, iron overload, SLC7A11/GSH/GPX4 axis, and ATF4/HSPA5/GPX4 axis. For the first time, we discovered that Fer-1 can ameliorate the toxicity of 4-tBP, which needs more investigations. Our results provided a scientific basis of molecular mechanism of 4-tBP-induced fish poisoning.

Chemists find shortcut to puffer fish poison

Chemists find shortcut to puffer fish poison

Chemists find shortcut to puffer fish poison

Chemists find shortcut to puffer fish poison

Gambierdiscus and Its Associated Toxins: A Minireview.

Gambierdiscus is a dinoflagellate genus widely distributed throughout tropical and subtropical regions. Some members of this genus can produce a group of potent polycyclic polyether neurotoxins responsible for ciguatera fish poisoning (CFP), one of the most significant food-borne illnesses associated with fish consumption. Ciguatoxins and maitotoxins, the two major toxins produced by Gambierdiscus, act on voltage-gated channels and TRPA1 receptors, consequently leading to poisoning and even death in both humans and animals. Over the past few decades, the occurrence and geographic distribution of CFP have undergone a significant expansion due to intensive anthropogenic activities and global climate change, which results in more human illness, a greater public health impact, and larger economic losses. The global spread of CFP has led to Gambierdiscus and its toxins being considered an environmental and human health concern worldwide. In this review, we seek to provide an overview of recent advances in the field of Gambierdiscus and its associated toxins based on the existing literature combined with re-analyses of current data. The taxonomy, phylogenetics, geographic distribution, environmental regulation, toxin detection method, toxin biosynthesis, and pharmacology and toxicology of Gambierdiscus are summarized and discussed. We also highlight future perspectives on Gambierdiscus and its associated toxins.

Exploring the Health Impacts of Climate Change in Subsistence Fishing Communities throughout Micronesia: A Narrative Review

Abstract For many people living in low-income coastal communities, marine resources provide a crucial source of animal protein and are of major nutritional importance. However, because of various human-induced pressures, such as overfishing and poor resource management, marine resources are deteriorating at an unprecedented rate. Climate change effects this dynamic by contributing directly to marine resource deterioration and acting as an effect multiplier, worsening already-present problems in the systems. This deterioration threatens the viability of marine resources to support future food security demands and presents multiple health implications for coastal communities that rely upon these resources. This research used a narrative review to explore how the impacts of climate change are projected to impact human health and sustainable development throughout subsistence fishing communities. A case study approach focusing on the Pacific Ocean region of Micronesia was conducted to provide a practical indication of the future scenario applicable to other geographical regions across the globe. The results indicate that climate change is likely to exacerbate adverse health outcomes such as food insecurity, ciguatera fish poisoning, heatstroke, and mental health problems and that climate change may lead to the deterioration of traditional cultural practices. As the climate crisis is happening now and will be an issue extending into the foreseeable future, it is necessary to implement adaptation strategies, funding, and governance to limit global emissions, preserve marine resources, and support human well-being. Therefore, this research details adaptation strategies, such as diversifying fish catch and reviving traditional postharvest preservation methods, that may help communities adapt to climate change. Significance Statement The Sixth Assessment Report by the Intergovernmental Panel on Climate Change (2021) has indicated that marine resources are deteriorating at an unprecedented rate across all regions of the globe. As marine resources significantly contribute to feeding coastal–semisubsistence communities, it is beneficial to understand how this deterioration will affect human health. Thus, this research indicates that declines in marine resources may exacerbate the prevalence of food insecurity, ciguatera fish poisoning, heat-related illnesses, mental health problems, and chronic diseases within marine-dependent communities. Furthermore, this research details adaptation strategies such as diversifying fish catch, improving postharvest preservation methods, and offering counseling services, which may help support the health and well-being of marine-dependent communities.

Literature Review on the Type of Fish and Histamine-producing Bacteria Associated with Histamine Poisonings in Japan

Histamine poisoning has been reported worldwide. Improvements in refrigeration technology have led to a reduction in this food poisoning; however, it continues to occur. Misdiagnosis of fish allergies has compounded this problem and the number of patients subjected to histamine poisoning that are transported to the emergency ward because of anaphylactic shock-like symptoms should not be underestimated. We investigated incidents of histamine food poisoning in Japan from 1998 to 2020, and found that there were a mean 9.7 incidents/year and 195.3 cases/year. Facility-wise occurrence of the incidents per year was the highest in restaurants followed by lunch facilities, and these together accounted for approximately 70% of the incidents. Facility-wise total number of cases was the highest in lunch facilities followed by restaurants, and these together accounted for 80% of the cases. Fish associated with histamine poisoning were mainly tuna, marlin, and mackerel. Based on the current literature review, 23 genera of histamine-producing bacteria were isolated from fish purchased in Japan. The most frequently reported bacteria were Morganella morganii and Photobacterium damselae. Psychrophilic bacteria such as Morganella psychrotolerans and Photobacterium phosphoreum were also isolated. To prevent histamine poisoning, freezing or fast handling of fish and the products during processing and consuming is important because only refrigeration of fish is enough.

Phytochemical and pharmacological review of Barringtonia racemosa

Barringtonia racemosa is a mangrove associated species plant belonging to the Lecythidaceae family. Barringtonia racemosa L. is commonly known as Powder-puff Mangrove Associate, putat, fish poison tree. It is widely distributed in Eastern Africa and Asia. This review discusses about Barringtonia racemosa ethnobotany, traditional uses, phytochemistry and pharmacological application.

Molecular characterization of toxic benthic dinoflagellate, Prorocentrum lima in west Indonesian waters using LSU 28S rDNA gene

Abstract. Widiarti R, Zamani NP, Bengen DG, Madduppa H. 2022. Molecular characterization of toxic benthic dinoflagellate, Prorocentrum lima in west Indonesian waters using LSU 28S rDNA gene. Biodiversitas 23: 3257-3263. Prorocentrum lima is one of the toxic benthic dinoflagellates known to cause Diarrhetic Shellfish Poisoning (DSP), which is also associated with ciguatoxin-producing species, Gambierdiscus toxicus that causes Ciguatera Fish Poisoning (CFP). P. lima has a wide range of morphological variability and genetic diversity, but such research has never been reported from Indonesian waters yet. This study aimed to determine the molecular characteristics of P. limain west Indonesian waters, namely Bintan Island, Belitung Island, Seribu Islands, and Karimunjawa Islands. Molecular characterization was conducted by amplification on large subunit (LSU)28S rDNA gene. Extraction was conducted using freeze-thaw which was continued with single cell PCR method. Genetic distance values and phylogenetic analysis were analyzed using MEGA software. Based on molecular analysis, P. lima from this research was divided into two subclades, namely subclade A from Seribu Islands and Belitung Island, and subclade B from Karimunjawa Islands and Bintan Island. P. lima from Bintan Island showed a closer relationship with the reference sequence from the Genbank. Observation of molecular characters of P. lima showed that the genetic diversity of P. lima depended on the variation of the island’s morphogenesis type.These findings could support a further study on the distribution of P. lima in Indonesian waters, related to the genetic variation and toxin production, since Indonesia consists of many small islands.

Indigenous Community Fishing Practices in Nagaland, Eastern Indian Himalayas

The significance of indigenous knowledge under the current scenario of biodiversity imperilment is well-known since such knowledge is gained through continuous intergenerational observations of natural systems. In this study, we present a description of indigenous community fishing practices in Nagaland and investigate their relationship with the cultural and traditional aspects of the associated communities through oral interactions, questionnaires and as a participatory observer. We observed inter- and intra-community fishing in which the piscicidal plants Millettia pachycarpa and Derris elliptica (both Fabaceae) were used as fish poison. M. pachycarpa was commonly used in inter-community ‘fishing festivals’ since it is easily available, less laborious to collect and there are no reports of harm to the body in comparison to D. elliptica which causes allergy and/or dermatological effects. Indigenous community fishing is conducted to develop a sense of peaceful co-existence and prosperity within and among the neighboring communities. However, the increasing use of synthetic fish poisons has overlapped with the traditional practices of fishing, exerting pressure on the livelihoods and food security of the tribal populations while contributing to riverine ecosystem degradation. Formulation of policies banning synthetic fish poison, and judicious use of traditional piscicidal plant fishing is therefore recommended.

Toxic Effects and Tumor Promotion Activity of Marine Phytoplankton Toxins: A Review.

Phytoplankton are photosynthetic microorganisms in aquatic environments that produce many bioactive substances. However, some of them are toxic to aquatic organisms via filter-feeding and are even poisonous to humans through the food chain. Human poisoning from these substances and their serious long-term consequences have resulted in several health threats, including cancer, skin disorders, and other diseases, which have been frequently documented. Seafood poisoning disorders triggered by phytoplankton toxins include paralytic shellfish poisoning (PSP), neurotoxic shellfish poisoning (NSP), amnesic shellfish poisoning (ASP), diarrheic shellfish poisoning (DSP), ciguatera fish poisoning (CFP), and azaspiracid shellfish poisoning (AZP). Accordingly, identifying harmful shellfish poisoning and toxin-producing species and their detrimental effects is urgently required. Although the harmful effects of these toxins are well documented, their possible modes of action are insufficiently understood in terms of clinical symptoms. In this review, we summarize the current state of knowledge regarding phytoplankton toxins and their detrimental consequences, including tumor-promoting activity. The structure, source, and clinical symptoms caused by these toxins, as well as their molecular mechanisms of action on voltage-gated ion channels, are briefly discussed. Moreover, the possible stress-associated reactive oxygen species (ROS)-related modes of action are summarized. Finally, we describe the toxic effects of phytoplankton toxins and discuss future research in the field of stress-associated ROS-related toxicity. Moreover, these toxins can also be used in different pharmacological prospects and can be established as a potent pharmacophore in the near future.

In vivo subchronic effects of ciguatoxin-related compounds, reevaluation of their toxicity

Ciguatoxins are marine compounds that share a ladder-shaped polyether structure produced by dinoflagellates of the genus Gambierdiscus and Fukuyoa, and include maitotoxins (MTX1 and MTX3), ciguatoxins (CTX3C) and analogues (gambierone), components of one of the most frequent human foodborne illness diseases known as ciguatera fish poisoning. This disease was previously found primarily in tropical and subtropical areas but nowadays, the dinoflagellates producers of ciguatoxins had spread to European coasts. One decade ago, the European Food Safety Authority has raised the need to complete the toxicological available data for the ciguatoxin group of compounds. Thus, in this work, the in vivo effects of ciguatoxin-related compounds have been investigated using internationally adopted guidelines for the testing of chemicals. Intraperitoneal acute toxicity was tested for maitotoxin 1 at doses between 200 and 3200 ng/kg and the acute oral toxicity of Pacific Ciguatoxin CTX3C at 330 and 1050 ng/kg and maitotoxin 1 at 800 ng/kg were also evaluated showing not effects on mice survival after a 96 h observation period. Therefore, for the following experiments the oral subchronic doses were between 172 and 1760 ng/kg for gambierone, 10 and 102 ng/kg for Pacific Ciguatoxin CTX3C, 550 and 1760 ng/kg for maitotoxin 3 and 800, 2560 and 5000 ng/kg for maitotoxin 1. The results presented here raise the need to reevaluate the in vivo activity of these agents. Although the intraperitoneal lethal dose of maitotoxin 1 is assumed to be 50 ng/kg, without chemical purity identifications and description of the bioassay procedures, in this work, an intraperitoneal lethal dose of 1107 ng/kg was obtained. Therefore, the data presented here highlight the need to use a common procedure and certified reference material to clearly establish the levels of these environmental contaminants in food.

Pharmacological studies of oil sardine fishes

The current review article discusses the toxins of different species of fish as well as their pharmacological and therapeutic applications. Fishing in coastal areas is a major concern because it can cause serious poisoning to fishermen, residents and visitors. Toxins from fish can cause severe discomfort, which can spread to the extremities and the lymphatic system. It may also result in venous stasis, hemorrhage, and alterations in the diameter of the artery wall. Particle channels, ligand-gated channels, and G-protein-coupled receptors in body cells are totally impacted by fish poisons as far as physiological and metabolic exercises. They damage the signaling molecules, causing hemolysis, cardiovascular problems, impaired nerve activity and smooth muscle contraction. The effects of fish venom on specific antibodies to the toxin are used for rapid neutralization. It reduces pain, reduces symptoms and stops the inflammatory process in its tracks. Fish poisons have a wide range of medicinal uses and their biological information can be used to develop immunological diagnoses, biocides, anticancer drugs and analgesics.