Feeding Injury Research Articles

Flea Beetle (Coleoptera: Chrysomelidae) Populations, Effects of Feeding Injury, and Efficacy of Insecticide Treatments on Eggplant and Cabbage in Southwest Virginia.

Flea beetles, are common pests of cabbage Brassica oleracea L. (Brassicales: Brassicaceae) and eggplant Solanum melongena L. (Solanales: Solanaceae), but little is known about the flea beetle populations in Virginia, their impact on yield, or the most effective control methods. This research investigates flea beetle populations and the impact of their feeding injury on cabbage and eggplant in Southwest Virginia and determines the most efficacious control methods. In Whitethorne, VA, cabbage and eggplant crops were vacuum sampled weekly throughout two summers (2015, 2016). Crucifer flea beetle, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae), and striped flea beetle, Phyllotreta striolata Fabr. (Coleoptera: Chrysomelidae) were found on cabbage; whereas, eggplant flea beetle, Epitrix fucula (Crotch) (Coleoptera: Chrysomelidae), and the tobacco flea beetle, Epitrix hirtipennis (Melsheimer) (Coleoptera: Chrysomelidae) were found on eggplant. To evaluate the impact of flea beetle feeding on these plants flea beetle densities and defoliation were assessed weekly and individual plant, as well as whole plot yields, assessed at harvest. For cabbage, significant yield reductions were observed between 1 and 20% and >60% defoliation. Similarly, significant yield reductions were observed between 41 and 60% and >60% defoliation for eggplant. The efficacy of various insecticides was also evaluated. Soil application of the systemic neonicotinoid dinotefuran, imidacloprid, and the foliar-applied bifenthrin resulted in the fewest beetles, the least amount of leaf defoliation, and the highest yield in cabbage and eggplant. This research helps vegetable growers to better understand the severity of these pests and how to effectively combat them.

Genetics of Thrips palmi (Thysanoptera: Thripidae)

Melon thrips (Thrips palmi) is the principal insect pest of vegetable and ornamental plants worldwide. In addition to inflicting feeding injuries on host plants, they act as vectors of economically damaging tospoviruses. Application of insecticides and host plant resistance has proven largely ineffective in the management of T. palmi. However, increasingly, genetic knowledge is being successfully utilized to manage insect pests. A number of recent studies have enriched the genetic database of T. palmi. In this review, we report on genetics of T. palmi for species identification and to study its populations structure. For example, a DNA polymorphism analysis of global T. palmi populations has revealed the existence of 29 haplotypes. The T. palmi population can be divided into three lineages based on phylogenetic analysis. A high maximum intraspecific distance is indicative of cryptic species within T. palmi populations. Regarding climate adaptability, the upregulation of trehalose biosynthesis genes, and genes encoding several heat-shock proteins is expected to enable T. palmi thermal adaptation to both cold and hot conditions. Genetics of T. palmi has shown that resistance to conventional insecticides like cypermethrin appears to be linked to mutations in the sodium channel. On the other hand, resistance to imidacloprid appears to be a result of cytochrome P450-mediated detoxification. T. palmi genes potentially involved in tospovirus transmission are also reviewed and new molecular tools for genetic study presented. Molecular modeling and docking analyses of groundnut bud necrosis virus glycoprotein demonstrated protein–protein interactions with T. palmi vacuolar ATP synthase E subunit, cathepsin, clathrin, adaptor protein 2, and enolase. Overall, a better knowledge of T. palmi genes will assist elucidation of thrips identification, means of adaptation in diverse ecological niches, mechanisms of insecticide resistance, and transmission of plant viruses. This data will be an invaluable tool for integrated thrips management.

Development, survival, and feeding behavior of Helicoverpa zea (Lepidoptera: Noctuidae) relative to Bt protein concentrations in corn ear tissues.

The corn earworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), preferentially oviposits and feeds on ears of corn (Zea mays L.) and can be managed using transgenic hybrids that produce insecticidal proteins from the bacterium Bacillus thuringiensis (Bt). Concentrations of Bt proteins can vary spatially and temporally in plant tissues, creating a heterogeneous environment that can increase the risk of resistance development. We planted small-plot trials of nine Bt and non-Bt corn hybrids in South Carolina in 2016 and 2017 and investigated the development, survival, feeding injury, and feeding behavior in corn ear tissues. ELISA was used to quantify the concentrations of Cry1F and Cry2Ab2 in young silk, old silk, maternal tip tissue, kernels, and husk. Cry1F and Cry2Ab2 significantly varied with silk age and both proteins were generally highest in the silk and tip tissue. Hybrids with pyramided proteins significantly reduced feeding injury to the silk, tip, and kernel ear tissues, which was less apparent with single Bt protein hybrids. The pyramided hybrid expressing Vip3A incurred no injury to either the ear tip or kernels, and only eight 1st instar larvae were collected in the silk of 520 sampled ears. Age of larvae significantly varied among ear tissues but not between hybrids. Depending on hybrid family, mean larval instar in the silk, tip, and kernels was 1st or 2nd, 3rd, and 5th, respectively. Instar-specific feeding penetrance into corn ears increased with age but did not differ between hybrids. We characterized the instar- and tissue-specific feeding behavior of H. zea larvae but did not detect differences in feeding behavior between Bt and non-Bt hybrids. Implications for resistance management strategies such as seed mixtures are discussed.

A predatory mite, Amblyseius swirskii, and plastic mulch for managing melon thrips, Thrips palmi, in vegetable crops

Melon thrips, Thrips palmi Karny (Thysanoptera: Thripidae), is a significant pest of vegetable crops and ornamental plants due to feeding and oviposition injury to vegetative and reproductive tissues. As an integrated approach, we evaluated the efficacy of a phytoseiid mite, Amblyseius swirskii Athias-Henriot, and different colored and UV-reflective plastic mulches for managing T. palmi in snap bean (Phaseolus vulgaris L.), cucumber (Cucumis sativus L.), yellow squash (Cucurbita pepo L.), eggplant (Solanum melongena L.), Jalapeño pepper (Capsicum annuum L.), and tomato (Solanum lycopersicum L.). The five mulch treatments evaluated were: “Shine N′ Ripe” (Metalized top and black bottom), “Can-Shine” (Metalized top and white bottom), “Black” plastic black-on-black (Can-Grow-XSB), black-on-white (Can-Grow XSB), “White” plastic; white-on-black (Can-Grow XSB), and bare soil with no mulch. The number of T. palmi adults and larvae in leaf samples collected from the middle third (stratum) of plants in each treatment was determined. In 2015 at 49 days after planting (DAP), curative release of 40–50 A. swirskii per plant did not suppress T. palmi effectively when the thrips population was high. There were no significant interactions between A. swirskii and crop or A. swirskii and mulch type on the number of T. palmi. However, in 2016 at 15 DAP, preventive release of A. swirskii, reduced the number of T. palmi in each of the mulch and crop treatments. Metalized mulch reduced the number of T. palmi early in the season when plants’ canopy did not shade the mulched area. These results suggest that use of metalized reflective mulch and A. swirskii, each have the potential to manage T. palmi at low population densities.

Effect of pre-harvest exposures to adult Halyomorpha halys (Hemiptera: Pentatomidae) on feeding injury to apple cultivars at harvest and during post-harvest cold storage

The effect of exposing apples to brown marmorated stink bug, Halyomorpha halys, for discrete intervals before harvest and of post-harvest cold storage on feeding injury expression was evaluated in 2011 and 2012. Individual apples from four cultivars in experimental orchards in Virginia and West Virginia, USA were caged soon after fruit set to protect them from insect injury. During each of the four weeks preceding harvest of each cultivar, five adult H. halys were placed in a subset of cages for 7-days, then removed. Control fruit were not exposed. The proportion of injured fruit and the number of external injuries was evaluated at harvest, after which the fruit were held in cold storage for about 5 weeks, followed by assessments of the proportion of fruit injured and the number of external and internal injuries. Most exposure timings resulted in external injury at harvest, but fruit exposed closer to harvest tended to show less injury than those exposed earlier. Fruit from all cultivars showed external injury at harvest, with variation in the proportion of injured fruit among them. The proportion of injured fruit and the number of external injuries tended to increase during post-harvest cold storage in some, but not all cultivars. The number of external injuries at harvest and after cold storage underrepresented the number of internal injuries. Results are discussed in the relation to the length of pre-harvest protection required to mitigate fruit injury from H. halys.

The effect of hook type and trailing gear on hook shedding and fate of pelagic stingray (Pteroplatytrygon violacea): New insights to develop effective mitigation approaches

The pelagic stingray (Pteroplatytrygon violacea) in the French Atlantic bluefin tuna makes up almost half of the catch in numbers, ranking first of the five major species caught. Given the high levels of catches, more attention was given to the impact of this fishery in order to avoid future conservation issues. The effects of the hook shape (circle versus J-type hooks) and trailing gear on hook retention has been investigated on 10 individuals kept in captivity during 125 days. Experiments showed that the J-type hook used commonly by fishers had a fast self-shedding rate which will allow for a quick resumption of feeding and minimal injury which means quicker wound healing and better chance for survival. J-type hooks were all expelled within 6 days while circle hook shedding rates were much longer, taking 44.5 ± 54.4 days (mean ± SD). The mechanism of expulsion of the hook has been clearly described and the impact of the trailing line assessed. Appropriate handling practices maximizing the crew safety and the post-release survival were identified. Other effective mitigation approaches for the fishery are proposed and discussed.

Evaluation of injury caused by lace bug, Cochlochila bullita (Stål) (Hemiptera: Tingidae) on Cat’s whiskers, Orthosiphon aristatus (Blume) Miq. and Sweet basil, Ocimum basilicum Linnaeus

The Ocimum Tingid, Cochlochila bullita (Stal) (Hemiptera: Tingidae) is an important Hemipteran pest for various economically important plants. This study focused on assessing the feeding injury caused by C. bullita on Cat’s whiskers, Orthosiphon aristatus (Blume) Miq. and Sweet basil, Ocimum basilicum Linnaeus. The injured area and number of faecal spots on the leaves caused by C. bullita were recorded. Leaves chlorophyll content were measured to examine the effect of C. bullita feeding on the leaf chlorophyll content. Result showed that feeding occurred on both sides of the leaves and the damages were clearly apparent on the upper leaf surface. The injured area was more in O. aristatus, 12.89 ± 0.52% compared to O. basilicum, 5.85 ± 0.37%. Chlorophyll reduction in infested O. aristatus was also more, with −6.40 ± 0.17 μg / cm2 compared to O. basilicum with only −3.13 ± 0.11 μg / cm2 after 3 days of infestation. Oviposition of C. bullita into midribs were observed and it nearly blocked the vascular tissues. However, these insertions seemed not to harm the plant in short-term, but damage occurs when the nymphs hatch and feed the plants. Overall, O. aristatus seems to be less tolerant to C. bullita infestation as compared to O. basilicum. Beyond the aesthetic damage caused by C. bullita on O. arisatus, severe reduction of chlorophyll due to feeding-induced stress complicated the physiological process of the plant.

Injury by Contarinia (Diptera: Cecidomyiidae) to early-seeded and late-seeded canola in northeastern Saskatchewan, Canada, and assessment of seed treatments for midge control

AbstractA three-year field study in northeastern Saskatchewan, Canada, determined the effects of seeding date and seed treatment on feeding injury by Contarinia Róndani (Diptera: Cecidomyiidae) midges to canola, Brassica napus Linnaeus (Brassicaceae), and subsequent seed yield. Emergence cage monitoring indicated the presence of two generations of midges at the four locations observed. Intensity of midge injury to canola was low, but damaged plants were common. Field location and seeding date influenced probability of midge injury and canola growth stage. The wettest of the four locations had the greatest number of midges but low levels of probability of damage in two of three years. Early-seeded plots had higher injury probability than late-seeded plots, with the highest probability of injury occurring one to two weeks after flowering commenced. Seed treatment marginally affected midge injury ratings, suggesting that insecticide efficacy dissipated before the midges attacked. Seed yield was higher in early-seeded than in late-seeded plots in two of three years, despite the greater level of damage seen in early-seeded plots. Overall, the research showed that the agronomic benefits of early seeding outweighed the effects of the midge damage observed; therefore, producers in the Canadian prairie provinces should seed canola when conditions are most agronomically suitable.

Evaluation of Mexican Bean Beetle (Coleoptera: Coccinellidae) Host Selection, Survival, and Feeding Injury Among Snap Bean and Lima Bean Cultivars.

Mexican bean beetle, Epilachna varivestis Mulsant (Coleoptera: Coccinellidae), is a serious pest of snap bean, Phaseolus vulgaris L. (Fabales: Fabaceae), lima bean, Phaseolus lunatus L. (Fabales: Fabaceae), and an occasional pest of soybean, Glycine max (L.) Merr. (Fabales: Fabaceae), in the United States. Past research indicates that some bean cultivars endure greater injury from this pest than others, suggesting that such crops are either more preferred, better hosts, and/or have poorer defenses. This study characterizes injury potential among three snap bean and three lima bean cultivars by measuring ovipositional preference, survival, and feeding injury of E. varivestis in field and greenhouse experiments. Snap beans, as a group, were more preferred and susceptible to injury than lima beans. The purple wax snap bean, Dragon's Tongue, was the most susceptible overall; while the dwarf bush lima bean, Henderson, was the least susceptible. The results of this study will aid in the development of integrated pest management strategies for E. varivestis, while also providing broader biological information for future research on this pest and others.

Impact of Lygus hesperus (Hemiptera: Miridae) Feeding and Oviposition on Lettuce

The western tarnished plant bug, Lygus hesperus Knight (Hemiptera: Miridae), is an important insect pest of lettuce, Lactuca sativa L. in the central coast region of California. However, little is documented on the injury symptoms relative to levels of L. hesperus infestation on lettuce. Experiments were conducted in which lettuce plants were exposed to 0, 1, 3, 5, and 10 L. hesperus adults for 2 and 7 days. The major injury symptom observed was lesions on the midrib of the leaves, and under low to moderate infestation (e.g., one to five adults per plant) after 2 days of exposure these lesions were not significantly different from the insect-free control. Under heavy infestation (e.g., 10 adults per plant), however, lesions developed rather quickly after 2 days of exposure. The number of lesions was positively associated with numbers of feeding sites and eggs. When heavy infestation lasted for a week, most of the plants died. At low to moderate infestation, more lesions developed compared with the insect-free control. After a weeklong exposure, an increase in number of lesions was negatively associated with plant height. The results emphasize the need for regular scouting to facilitate immediate management decisions and actions to reduce losses from L. hesperus feeding and oviposition injury in lettuce.

Effects of Planting Date on Thrips (Thysanoptera: Thripidae) in Cotton.

At-plant applications of insecticides are the most common method to manage thrips in upland cotton, Gossypium hirstutum L. Because the primary pest species, tobacco thrips, Frankliniella fusca (Hinds), has developed resistance to commonly used neonicotinoid insecticides used in producing cotton, alternative control options are needed for sustainable thrips management programs. A 3-year study (2015-2017) showed that densities of thrips, feeding injury from thrips, cotton growth, and yield varied among 10 planting dates. Densities of thrips were lowest in seedling cotton planted after mid-May in all years. Thrips injury ratings in all years were highest in cotton planted in April, lowest in cotton planted in June, and below intermediate injury (intermediate corresponded to a 3 on the 0-5 scale) levels in cotton planted after mid-May. Cotton planted during May, rather than in April or June, had the highest yield potential, regardless of variety. Results of the study indicated that altering planting date could potentially be useful in mitigating injury and losses from thrips in upland cotton.

Efficacy of Chlorantraniliprole Seed Treatments Against Armyworm (Mythimna unipuncta [Lepidoptera: Noctuidae]) Larvae on Corn (Zea mays).

Mythimna unipuncta (Haworth) (Lepidoptera: Noctuidae) is an important insect pest of corn (Zea mays L.) in North America and can cause severe damage during outbreaks. Insecticides are the main control method; however, development of insecticide resistance poses management challenges and necessitates the use of novel insecticides. A synthetic insecticide, chlorantraniliprole, belonging to the anthranilic diamides, targets insect ryanodine receptors and is a potential alternative to conventional insecticides for management of M. unipuncta. We determined the efficacy of seed treatment with chlorantraniliprole alone compared with a positive control consisting of a neonicitinoid seed treatment of thiamethoxam and combinations of both compounds against M. unipuncta larvae in laboratory bioassays. Bioassays were conducted on two different growth stages of M. unipuncta larvae (instars 3 and 5) and two plant growth stages (V1 and V2 corn) in clip cages and whole plant experiments. Larval mortality, head capsule width, and feeding injury were measured. The chlorantraniliprole seed treatment alone or in combination with thimethoxam at different doses affected survival of M. unipuncta larvae. In all bioassays except one, larval mortality occurred earlier when a combination of chlorantraniliprole and thimethoxam seed treatment was used when compared with a thimethoxam alone seed treatment. Mythimna unipuncta larvae developed faster on the untreated control corn plants compared with corn with insecticide seed treatments. Foliar injury was low in both chlorantraniliprole alone and chlorantraniliprole + thimethoxam treatments compared with the control. Chlorantraniliprole thus offers potential alternative to conventionally used insecticides in the management of M. unipuncta in corn.

Deriving Economic Models for Pea Aphid (Hemiptera: Aphididae) as a Direct-Pest and a Virus-Vector on Commercial Lentils.

The pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera: Aphididae), presents a dual threat to commercial pulse growers because it can inflict direct injury through feeding and indirect injury as a vector of two important viruses, Pea enation mosaic virus (PEMV) and Bean leafroll virus (BLRV). A decision support system is needed to help producers manage both of these threats in pulses. To address these gaps in lentil, Lens culinaris (Medikus) (Fabales: Fabaceae), we conducted field experiments near Moscow, Idaho in 2011 and 2012 with three objectives: 1) determine economic injury levels (EILs) for pea aphid in lentil based on the direct effects of their feeding on yield, 2) develop economic guidelines for treating aphids carrying PEMV or BLRV based on the impact on yield of virus inoculation at different times after crop emergence, and 3) provide a framework for using both of these decision tools as part of a comprehensive approach to pea aphid management in lentil. EILs were determined based on data from replicated field cage trials over 2 yr. Windows of economic vulnerability to viruses were determined based on artificial inoculation with viruses at different days after crop emergence over 2 yr. Both direct and indirect injury support tools can be parameterized with potential yields, market prices, and the costs of insecticide applications to guide treatment decisions. Together, the two tools comprise a decision support system for managing pea aphid acting as both a direct pest and as a vector of the viruses in lentils in the Palouse region of northern Idaho and southeastern Washington State.

Lethal and Sublethal Effects of Organically-Approved Insecticides againstBagrada hilaris(Hemiptera: Pentatomidae)1

Abstract Bagrada hilaris (Burmeister) (Hemiptera: Pentatomidae) is a stink bug species that preferentially attacks cruciferous crops. Because organic growers have limited insecticide tools for combating B. hilaris, the lethal and sublethal effects of organically approved insecticide products and their selected combinations against B. hilaris were examined in laboratory and field settings. In a topical spray assay, spinosad, either alone or in combination with pyrethrins, azadirachtin, and potassium salts, resulted in >95% mortality relative to the water-only treatment 48 h postapplication; whereas, stand-alone pyrethrins and azadirachtin failed to cause similar mortality rates. When adults were exposed to treated broccoli (Brassica oleracea var. italica Blenck) seedlings for 48 h after 0, 1, and 7 d of application, the number of feeding injury sites was significantly lower with the combined treatment of spinosad, pyrethrins, and azadirachtin than those on seedlings treated with water alone at 0 and 1 d p...

Does <i>Pestalotiopsis royenae</i> Cause Leaf Streak of Large Cardamom?

Leaf streak caused by Pestalotiopsis royenae (Guba) Steyaert has been reported as a new disease of large cardamom ( Amomum subulatum ). It is characterized by rectangular spots running parallel to the veins. P. royenae was isolated onto potato dextrose agar from the infected portion of the plant. In the pathogenicity test, disease symptoms were not present on inoculated plant and on detached leaf in vitro after 20 days. During 2014–2016, a survey in various large cardamom plantations of Sikkim revealed the presence of tea mosquito bugs on the infected parts of A. subulatum . The spots did not show any growth. Different sized spots have been noticed on the infected leaf produced by different instars. At the initial stage, these symptoms appear on the young and tender leaves of large cardamom. Tea mosquito bugs were collected from the infested leaves and allowed to feed under controlled conditions, which produced similar type of leaf streak symptoms in large cardamom and also in other non-host crops like maize and turmeric. The results reveal that the cause of leaf streak is due to feeding injury of tea mosquito bugs and rule out P. royenae as a pathogen causing leaf streak disease symptoms. Pestalotiopsis sp. was also isolated as endophyte from large cardamom.

Avoiding Unwanted Vicinity Effects With Attract-and-Kill Tactics for Harlequin Bug, Murgantia histrionica (Hahn) (Hemiptera: Pentatomidae).

In the development of an attract-and-kill approach for the management of harlequin bug (HB), Murgantia histrionica (Hahn), we evaluated attraction and retention of HB by pheromone-baited traps in the field. In release-recapture and on-farm experiments, traps with collard plants with lures-containing HB aggregation pheromone (murgantiol = 10,11-epoxy-1-bisabolen-3-ol) arrested and retained more HB than traps with either plant or lure. In order to avoid unwanted vicinity effects (increased feeding injury to neighboring crop plants due to halo or spillover effects), we also investigated two methods of retaining HB that were attracted to traps: a systemic toxicant (neonicotinoid applied to the trap plant as a drench) and a contact toxicant (long-lasting insecticidal netting [LLIN] with incorporated pyrethroid). More HB adults and more HB-feeding injury were observed on collard plants in the vicinity of lures compared with those neighboring lures in combination with a systemic toxicant. This difference indicates that improvements to trap retention acted to mitigate spillover effects, thereby avoiding unwanted vicinity effects. We also conducted laboratory assays in order to estimate the length of exposure to LLIN necessary to knock down HB adults and nymphs, calculating a knockdown time (KDT50) of 78.3, 2.6, and 2.1 s for females, males, and nymphs, respectively.

Evaluation of Alternatives to an Organophosphate Insecticide with Selected Cultural Practices: Effects on Thrips, Frankliniella fusca, and Incidence of Spotted Wilt in Peanut Farmscapes.

Peanut growers use a combination of tactics to manage spotted wilt disease caused by thrips-transmitted Tomato spotted wilt virus (TSWV). They include planting TSWV-resistant cultivars, application of insecticides, and various cultural practices. Two commonly used insecticides against thrips are aldicarb and phorate. Both insecticides exhibit broad-spectrum toxicity. Recent research has led to the identification of potential alternatives to aldicarb and phorate. In this study, along with reduced-risk, alternative insecticides, we evaluated the effect of conventional versus strip tillage; single versus twin row seeding pattern; and 13 seed/m versus 20 seed/m on thips density, feeding injury, and spotted wilt incidence. Three field trials were conducted in Georgia in 2012 and 2013. Thrips counts, thrips feeding injuriy, and incidence of spotted wilt were less under strip tillage than under conventional tillage. Reduced feeding injury from thrips was observed on twin-row plots compared with single-row plots. Thrips counts, thrips feeding injury, and incidence of spotted wilt did not vary by seeding rate. Yield from twin-row plots was greater than yield from single-row plots only in 2012. Yield was not affected by other cultural practices. Alternative insecticides, including imidacloprid and spinetoram, were as effective as phorate in suppressing thrips and reducing incidence of spotted wilt in conjunction with cultural practices. Results suggest that cultural practices and reduced-risk insecticides (alternatives to aldicarb and phorate) can effectively suppress thrips and incidence of spotted wilt in peanut.

Effects of Herbal-cake-separated Moxibustion on Blood Lipid Protein Levels and Expression of Toll-like Receptor and Nuclear Factor Genes in Atherosclerotic Plaques in Hyperglycemia Rabbits

To observe the effect of herbal cake-separated moxibustion on blood lipid-apoprotein levels and the expression of Toll-like receptor 2 (TLR 2), TLR 4 and nuclear factor kappa B(NF-κB) mRNAs in atherosclerotic (AS) vulnerable plaques of hyperglycemia rabbits, so as to explore its mechanism underlying improvement of atherosclerosis. Sixty New Zealand rabbits were randomly divided into 5 groups: control, model, direct moxibustion, herbal-cake-separated moxibustion and medication groups(n＝12 rabbits in each group). The AS vulnerable plaque model was established by high-fat forage feeding plus balloon-induced abdominal aorta injury and gene transfection of Ad 5-p 53 recombinant vector. Direct moxibustion or herbal-cake-separated moxibustion was applied to "Juque" (CV 14) and bilateral "Tianshu" (ST 25), "Fenglong" (ST 40), or bilateral "Xinshu" (BL 15), "Pishu" (BL 20) and "Ganshu" (BL 18) for 15－20 min every time. The medication group was treated by feeding Atorvastatin. All the treatments were conducted once daily for 8 weeks. Plasma total cholesterol(TC) and triglyceri-de(TG) contents were detected by enzyme method, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) contents detected by colorimetric determination, and plasma apolipoprotein A(Apo-A) and apolipoprotein B(Apo-B) levels determined by electrophoretic method. The pathological changes of vulnerable plaque and the aortic intima and media thickness were observed under light microscope after H．E. staining. The expression levels of TLR 2, TLR 4 and NF-κB mRNAs in AS plaques were determined by quantitative real-time PCR. After modeling, the levels of plasma TC, TG, LDL and Apo-B in the model group were remarkably increased (P<0.01), and Apo-A and HDL/LDL were significantly decreased in comparison with the control group (P<0.01). Additionally, the aortic intima and media thickness and the expression levels of TLR 2, TLR 4 and NF-κB mRNAs in AS plaques were significantly increased (P<0.01). After the treatment, the elevated levels of plasma TC, TG, LDL and Apo-B, the aortic intima thickness and media thickness, and the expression levels of TLR 2, TLR 4 and NF-κB mRNAs in the 3 treatment groups were significantly down-regulated in comparison with the model group (P<0.05, P<0.01), while the decreased levels of Apo-A and HDL/LDL were considerably increased (P<0.01). Comparison among the 3 treatment groups showed that the therapeutic effects of the herbal-cake-separated moxibustion and medication were significantly superior to those of the direct moxibustion in down-regulating the levels of TC, TG, LDL, Apo-B, TLR 2 mRNA, TLR 4 mRNA and NF-κB mRNA, and reducing the thickness of the aortic intima and media, as well as in up-regulating the levels of Apo-A and HDL/LDL (P<0.05, P<0.01). No significant differences were found between the herbal-cake-separated moxibustion and medication groups in the above-mentioned indexes (P>0.05). Herbal-cake-separated moxibustion has a positive role in stabilizing AS vulnerable plaque in hyperglycemia rabbits, which may be associated with its effects in regulating blood lipid-apolipoprotein levels and inhibiting the expression of TLR 2, TLR 4 and NF-κB mRNAs in vulnerable plaques.

Potential biopesticides for crucifer flea beetle, Phyllotreta cruciferae (Coleoptera: Chrysomelidae) management under dryland canola production in Montana

The crucifer flea beetle, Phyllotreta cruciferae (Goeze), is an economically important and dominant pest of canola (Brassica napus L) in the Northern Great Plains of the USA. The current flea beetle management strategy is based on using synthetic chemical treated seeds and if necessary, foliar spray of chemicals at canola seedlings in early spring for targeting adult population. However, there is an increasing demand for development of alternative management strategies for P. cruciferae pertaining to concerns over the development of resistance to synthetic insecticides and non-target effects on pollinators and other beneficial insects. Replicated field trials were conducted to test the efficacy of several commercially available biopesticides including Entrust® (spinosad), entomopathogenic nematode Steinernema feltiae + Barricade® (polymer gel 1%), Aza-Direct® (azadirachtin), Pyganic 1.4® EC (pyrethrin), Grandevo® SC (Chromobacterium subtsugae), Venerate® XC (Heat killed Burkholderia sp. strain A396 as seed treatment and foliar application) and Gaucho® (imidacloprid) (chemical check) for the P. cruciferae management at two locations (Conrad and Sweetgrass) of Montana in 2016. Biopesticide products were evaluated based on canola leaf area injury ratings and seed yield levels. Although, there was no clear trend of canola yield increase, selected biopesticide treatments were effective in maintaining low leaf area injury ratings as compared to untreated control. Entrust was able to maintain low leaf area injury ratings (8.5–14.5%) when compared to untreated control (16.0–21.4%) at both the locations. Entomopathogenic nematodes, Steinernema feltiae + Barricade® and Venerate® applied as foliar treatments maintained significantly lower feeding injury pressure at Sweetgrass (11.8%) and Conrad (13.4%) locations respectively, when compared to the untreated control. Our study results suggest that these biopesticide treatment results were comparable in efficacy to the chemical seed treatment Gaucho®. Other two biopesticide products- Aza-Direct® and Pyganic 1.4® EC treatments did not provide effective control of P. cruciferae at both the locations.

Laboratory assessment of feeding injury and preference of brown marmorated stink bug, Halyomorpha halys Stål (Hemiptera: Pentatomidae), for Actinidia chinensis var. deliciosa ‘Hayward’ (Zespri® Green) and Actinidia chinensis var. chinensis ‘Zesy002’ (Zespri® SunGold)

ABSTRACTThe brown marmorated stinkbug (BMSB), Halyomorpha halys Stål (Hemiptera: Pentatomidae), is native to Asia and is characterised by its polyphagous feeding habits and high hitchhiking potential. In invaded areas, such as the eastern USA, economic damage to agricultural crops by BMSB has been significant. In northern Italy, where BMSB is invasive, feeding damage has been recorded in commercial kiwifruit orchards. In New Zealand, a major kiwifruit producer, BMSB originating from the USA, Italy and China (the native range of kiwifruit) have been intercepted. These BMSB interceptions pose a high biosecurity risk to key agricultural industries in New Zealand, including kiwifruit. However, information on the ability of BMSB to feed on key commercial kiwifruit varieties, and the types of damage it may cause to this crop, is lacking. To address this issue, Actinidia chinensis var. ‘SunGold’ (G3) and Actinidia deliciosa var. ‘Green’ (Hayward), were exposed to adult BMSB under no-choice and choice feeding trials. Across kiwifruit cultivars (i.e. Green and SunGold) and experimental setups (i.e. choice and no-choice), mixed adult groups (i.e. males and females feeding together) caused significantly more damage than individual females and males. After accounting for adult density, there was no experimental evidence that BMSB exhibited a feeding preference for either SunGold or Green varieties. However, there were variety differences for the development of BMSB feeding injury, with lower incidence of damage recorded for SunGold.