Protein Source In Feed Research Articles

Techno-Economic Analysis of Gas Fermentation for the Production of Single Cell Protein.

Despite the large carbon footprint of livestock production, animal protein consumption has grown over the past several decades, necessitating new approaches to sustainable animal protein production. In this techno-economic analysis, single cell protein (SCP) produced via gas fermentation of carbon dioxide, oxygen, and hydrogen is studied as an animal feed source to replace fishmeal or soybean meal. Using wind-powered water electrolysis to produce hydrogen and oxygen with carbon dioxide captured from corn ethanol, the minimum selling price (MSP) of SCP is determined to be $2070 per metric ton. An emissions comparison between SCP, fishmeal, and soybean meal shows that SCP has a carbon intensity as low as 0.73 kg CO2-equiv/kg protein, while fishmeal and soybean meal have an average carbon intensity of 2.72 kg CO2-equiv/kg protein and 0.85 kg CO2-equiv/kg protein, respectively. Moreover, SCP production would occupy 0.4% of the land per ton of protein produced compared to soybean meal and would disturb less than 0.1% of the marine ecosystem currently disturbed by fishmeal harvesting practices. These results show promise for the future economic viability of SCP as a protein source in animal feed and indicate significant environmental benefits compared to other animal feed protein sources.

Yellow mealworm (Tenebrio molitor) as an alternative protein source in fish feed

The rapid growth of aquaculture has increased the demand for fish meal and fish oil, essential components of fish feed, leading to higher production costs due to dwindling global supplies. As the industry seeks sustainable alternatives, insects, particularly the yellow mealworm (Tenebrio molitor), are emerging as a viable protein source. With a nutritional profile comparable to fish meal and low environmental impact, insects offer an efficient and economical solution for feed production. The European Union's approval of mealworms for both animal feed and human consumption highlights their potential to support food security and environmental sustainability amidst rising global protein demands.

Moringa leaf: A potential protein source for animal feed

Moringa leaf: A potential protein source for animal feed

α-Glucosidase Inhibitors from Cold-Pressed Black Sesame (Sesamum indicum) Meal: Characterization of New Furofuran Lignans, Kinetic Study, and In Vitro Gastrointestinal Digestion.

Black sesame (Sesamum indicum) meal is an agricultural waste obtained after oil extraction. It is used as a key protein source in animal feed. Previous investigations have indicated that its health benefits, such as antidiabetic activity, are mainly due to its high lignan content. In the present study, we applied α-glucosidase inhibitory guided isolation to identify the active components responsible for the above claim. Twenty-nine compounds, mostly lignans, were isolated and identified, of which five (2-3, 12-13, and 28) were newly isolated. Of the isolated compounds, 20 and 21 were the most potent inhibitors, retarding enzyme function in noncompetitive and uncompetitive manners. Structure-activity relationship analysis suggested that the number of phenolic hydroxyl groups in the structures was significantly related to the inhibitory effect against α-glucosidase. A gastrointestinal digestion study of the major lignan sesaminol triglucoside (STG, 9) suggested that the transformation of dioxymethylene and glucoside moieties gradually began in the late process, thus enhancing the α-glucosidase inhibitory effect.

The Potential of Oil Palm Plantation Byproducts as Feed for Beef Cattle

Oil palm is one of the plantation commodities that Indonesia relies on to generate foreign exchange annually. The oil palm industry generates both solid and liquid waste. Oil palm waste is a byproduct of the oil palm industry that is not reused; however, with technological advancements, this waste can be utilized as supplementary feed, especially for ruminants. The waste obtained from oil palm plantations and oil palm mills includes oil palm fruit bunches, oil palm fibers, oil palm shells, oil palm kernel meal, oil palm leaves, and oil palm sludge. Mucuna barcteata can also be used as a protein source for animal feed. Research was conducted in the Sei Mangkei plantation, and feed trials were carried out in the Bangun PTPN III Plantation (Simalungun). The methods employed included surveys and laboratory research. Proximate analysis of the suspected feed sources was conducted by PT Saraswanti Indo Genetech Bogor, which encompassed total energy, moisture and ash content, total fat, protein, carbohydrates, crude fiber, calcium, and phosphorus. The weight gain of cattle after being fed the experimental diet was observed over a three-month period. The research results indicate that oil palm byproducts can support a population of 12,365 head of beef cattle per year. Mucuna bracteata, oil palm kernel meal, oil palm fronds, and solid decanter can be used as animal feed. A feed mixture containing 30% Mucuna resulted in the highest weight gain in cattle compared to the other three treatments, with an average daily gain of 0.97.

Evaluation of the Application Value of Cottonseed Protein Concentrate as a Feed Protein Source in Broiler Chickens

Cottonseed protein concentrate (CPC) has the function of replacing soybean meal to maintain normal animal growth and development. This study involved 180 Arbor Acres (AA) broilers, which were randomly assigned to three different treatments. Each treatment had six replicates, with each replicate consisting of 10 chicks. The control group was fed a basal diet, while the CPC-1 and CPC-2 groups used CPC to replace 25% and 50% of the soybean meal in the basal diet, respectively. The study showed that replacing soybean meal with 25% CPC in broilers’ diets can maintain normal growth, while substituting 50% of soybean meal with CPC negatively affects the growth and development of broiler chickens. Furthermore, the CPC-1 group showed a significant increase in serum total antioxidant capacity, superoxide dismutase enzyme activity, and immunoglobulin content, along with a decrease in malondialdehyde content. Based on the research results mentioned above, it was speculated that CPC has the potential to replace around 25% of soybean meal in broiler feed without causing any negative impact on growth performance. This suggests that CPC could be a viable alternative to soybean meal in broiler diet.

Lethal and sublethal effects of chronic exposure to insecticide residues on reared Alphitobius diaperinus

Abstract Edible insects such as lesser mealworm (Alphitobius diaperinus) are a promising new protein source for food and feed. The feed substrate on which these insects are reared may be contaminated with residues of insecticides originating from agricultural products that may impact insect performance. In this study, two generations of A. diaperinus were chronically exposed to spinosad (2.0 and 0.2 mg/kg) and imidacloprid (0.1 and 0.01 mg/kg) in the substrate. The aim was to determine sublethal effects on performance measures (total biomass (yield), mean individual weight, number of alive individuals) of larvae, pupae, and adult beetles, as well as pupation and eclosion. Exposure to spinosad at 2.0 mg/kg resulted in significant adverse effects on most performance measures of larvae, of both generations. Imidacloprid caused a reduction in yield and mean individual weight of the larvae as compared to the control at 0.1 mg/kg, while an increase in those measures was observed at 0.01 mg/kg. Significant adverse effects on adult beetles were only observed for imidacloprid at 0.1 mg/kg, and no significant effects of this insecticide on pupation and eclosion were observed. The concentrations of tested substances in larval samples were negligible for both generations, however, transfer from substrate to larval biomass was higher in the offspring generation relative to the parent generation. More research is needed to fully assess the hazard of insecticide residues to cause sublethal effects on A. diaperinus, for which method development for more cost-efficient designs is required.

Tracking digestible and non-digestible cell wall components during protein concentrate production from grass-clover and alfalfa

AbstractGreen biomass from leguminous and gramineous forage crops, such as alfalfa and grass-clover, has been proposed as a potential new source of feed protein concentrates for non-ruminant livestock. However, the efficient separation of the protein fraction from the non-digestible cell components, primarily comprising cell walls (fiber) and starch, presents a significant technological challenge. Moreover, it is crucial to optimize the process to preserve the optimal nutritional value of the final product. This study comprehensively analyzed the non-digestible fiber content and composition across all biorefinery fractions using two different feedstocks: green biomass from alfalfa and grass-clover. The pilot scale refining process involved a combination of screw pressing, lactic acid fermentation, and protein separation via centrifugation. We observed variations in carbohydrate composition and abundance between alfalfa and grass-clover. The lactic acid fermentation led to a reduction in cellulose and total glucose content. Our findings indicate that the final protein concentrate still contains residual cell wall components, including lignin, indicating potential inefficiencies in the filtration, fermentation, and isolation steps. The presented analytical approach provides a valuable framework for optimizing processing conditions and tailoring enzyme cocktails for enhanced valorization of the by-products.

Biochemical and protein nutritional potential of mulberry (Morus alba L.) leaf: partial substitution improves the nutrition of conventional protein.

With the requirements of environmental, cost and economic sustainability, new sources of alternative proteins in the livestock industry are receiving increasing attention. Mulberry (Morus alba L.) leaves are a unique feed resource because of their high protein content and large availability. Therefore, mining sustainable protein suitable for the animal husbandry industry in sericulture resources could achieve a win-win situation. The protein content in mulberry leaves is 232.10-386.16 g kg-1 , and the mean value of crude fat content is 43.76 ± 8.48 g kg-1 , which has the advantages of protein content and energy. In addition, the average content of phytic acid in mulberry leaves is only 1.88 ± 0.56 g kg-1 , which means that it is not inhibited in terms of nutrient absorption. Meanwhile, the digestibility of protein was Bean pulp > Sample 8 ≈ Alfalfa ≈ Sample 13 ≈ Cottonseed meal > Fish meal, and the β-turn and particle size of mulberry leaf protein are more conducive to digestion in vitro. Furthermore, the protein of Sample 13 had the richest essential amino acids (252.00 g kg-1 ) and the highest essential amino acid index (EAAI), which was superior to conventional feed protein. In addition, the partial substitution of mulberry leaf protein (15%) significantly increased the EAAI value of conventional feed protein. However, to balance nutrition, it is necessary to combine mulberry leaf protein with other proteins to further broaden its application field. Mulberry leaves are a new source of feed protein, which helps to alleviate the two major problems of mulberry resource surplus and feed protein resource shortage. © 2023 Society of Chemical Industry.

Effect of microbial fermentation on the quality of soybean meal

SummarySoybean meal is an important and inexpensive source of protein in food and animal feed, and contains anti‐nutritional factors such as trypsin inhibitors, antigenic proteins, oligosaccharides, phytic acids and lectins, which limit its application. Microbial fermentation has the capability to improve the nutritional value and biological activities of soybean meal by changing its natural composition. Fermentation results in the degradation of anti‐nutritional factors in soybean meal, an increased amount of small molecular protein peptides and improved content of isoflavone aglycone. However, the nutritional composition and biological activities of the resulting fermented products differ due to the different microbial species and conditions used for fermentation. Compared to soybean meal, fermented soybean meal has several beneficial effects such as digestibility, antioxidant and hypoallergenicity. The objective of this review is to discuss the fermentation processes of soybean meal, as well as outlines some biological activities associated with it. Furthermore, the current application of fermented soybean meal is also summarised.

PSI-3 Effects of Strategic Blending of Food Industry By-Products with Canola Meal on in Vitro and in Situ Fermentation and Nutrient Degradation

Abstract Canola meal (CM) has become an increasingly available high protein feed source for beef cattle in North America, thanks to the expansion of the canola crushing industry in Western Canada and the United States. The inclusion of CM in diets has been reported to improve nutrient utilization and the performance of growing beef cattle. However, the energy value of CM was not as high as barley grain finishing diets, resulting in poorer performance of finishing feedlot steers. Pea starch and pea molasses are available as food industry by-products of industrial pea protein extraction. However, the utilization of these food industry by-products as a component of livestock feed has not been well evaluated. The strategic blending of food industry by-products, such as pea starch and pea molasses, with canola meal (CM) is a novel approach to improving the energy density of CM as animal feed. Nutrient synchrony, where the energy and protein availability in the rumen is synchronized to maximize the ruminal microbial fermentation, has been proposed to improve animal performance. The objectives of our study were to evaluate the in vitro and in situ fermentation and nutrient degradability of novel blended CM. Pea starch and pea molasses were added at 5% (CM5) and 10% (CM10) levels in CM (DM basis), with regular CM serving as control (CON). The in vitro study consisted of two runs of 24 h incubations with three replicates per treatment. The in situ study utilized three cannulated beef heifers and used the same number of replicates of samples and time periods as for the in vitro study. Results indicated a numerical improvement in DM degradation for CM5 and CM10 than CON both in vitro and in situ incubations. Total gas production (mmol) was numerically greater, while the proportion of methane (mmol and g/DM) was less for CM5 than CON. Results indicated that in vitro fermentation of CM5 was enhanced, while greater propionate production from CM5 likely reduced methane production. Further evaluation of these food industry by-products for beef cattle growth performance studies could provide valuable information about rumen fermentation, nutrient digestibility, and energy value of these novel blended CM.

Black soldier fly larvae meal supplementation in a low protein diet reduced performance, but improved nitrogen efficiency and intestinal morphology of duck.

Reduced crude protein (CP) diets offer potential benefits such as optimized feed efficiency, reduced expenses, and lower environmental impact. The objective of this study was to evaluate black soldier fly larvae (BSFL) meal on a low-protein diet for duck performance, blood biochemical, intestinal morphology, gastrointestinal development, and litter. The experiment was conducted for 42 days. A total of 210-day-old male hybrid ducklings (5 replicate pens, 7 ducks per pen) were randomly assigned to 6 dietary treatments (3×2 factorial arrangements) in randomized design. The factors were CP level (18%, 16%, 14%) and protein source feed soybean meals (SBM), black soldier fly larvae meals (BSFLM). Reduced dietary CP levels significantly decreased growth performance, feed intake, the percentage of nitrogen, pH (p<0.05), and tended to suppress ammonia in litter (p = 0.088); increased lipid concentration; and enhanced relative weight of gastrointestinal tracts (p<0.05). In addition, dietary BSFL as a source of protein feed significantly increased lipid concentration and impacted lowering villus height and crypt depth on jejunum (p<0.05). In conclusion, the use of BSFLM in a low-protein diet was found to have a detrimental effect on growth performance. However, the reduction of 2% CP levels in SBM did not have a significant impact on growth performance but decreased nitrogen and ammonia concentrations.

Fractionation of proteins from alfalfa juice at demonstration scale: Effects of temperature and centrifugation strategies on removal of green protein and recovery of the soluble white protein fraction

Given the growing global demand for protein, alfalfa (Medicago sativa) has emerged as a promising alternative protein source for both animal feed and human nutrition due to its high protein content and nutritional value. This study investigated the fractionation of proteins in freshly pressed alfalfa juice into green and white fractions, with focus on optimizing the recovery of the white protein fraction, which holds significant potential for various food applications. Two sets of demonstration-scale experiments were conducted to evaluate the impact of temperature and centrifugation techniques on the fractionation process. The goal was to achieve the optimal coagulation and removal of the green fraction while concurrently enhancing the yield of the white protein fraction. The first set of experiments explored heat treatment at four different temperatures (50 °C, 55 °C, 60 °C, and 65 °C), followed by decanter centrifugation. Optimal coagulation of the green fraction with higher white protein fraction yield was observed at 55 °C. In the second set of experiments, a constant temperature was maintained (55 °C) while testing three different separation scenarios. A two-stage centrifugation approach employing decanter and disc-stack high-speed centrifuges proved most effective, removing most of the coagulated green fraction and preserving over 80% of the white fraction in the residual juice after heat treatment. Complete removal of the green fraction was not achieved, and an additional microfiltration step was introduced to clarify the residual juice. Microfiltration retained up to 50 % of the white protein, resulting in a significant reduction in the overall recovery yield. The results indicate that achieving high recovery yields of soluble white protein remains a challenge and requires further optimization of the fractionation process.

Cellulose-degrading bacteria improve conversion efficiency in the co-digestion of dairy and chicken manure by black soldier fly larvae

Black soldier fly larvae (BSFL) have potential utility in converting livestock manure into larval biomass as a protein source for livestock feed. However, BSFL have limited ability to convert dairy manure (DM) rich in lignocellulose. Our previous research demonstrated that feeding BSFL with mixtures of 40% dairy manure and 60% chicken manure (DM40) provides a novel strategy for significantly improving their efficiency in converting DM. However, the mechanisms underlying the efficient conversion of DM40 by BSFL are unclear. In this study, we conducted a holistic study on the taxonomic stucture and potential functions of microbiota in the larval gut and manure during the DM and DM40 conversion by BSFL, as well as the effects of BSFL on cellulosic biodegradation and biomass production. Results showed that BSFL can consume cellulose and other nutrients more effectively and harvest more biomass in a shorter conversion cycle in the DM40 system. The larval gut in the DM40 system yielded a higher microbiota complexity. Bacillus and Amphibacillus in the BSFL gut were strongly correlated with the larval cellulose degradation capacity. Furthermore, in vitro screening results for culturable cellulolytic microbes from the larval guts showed that the DM40 system isolated more cellulolytic microbes. A key bacterial strain (DM40L-LB110; Bacillus subtilis) with high cellulase activity from the larval gut of DM40 was validated for potential industrial applications. Therefore, mixing an appropriate proportion of chicken manure into DM increased the abundance of intestinal bacteria (Bacillus and Amphibacillus) producing cellulase and improved the digestion ability (particularly cellulose degradation) of BSFL to cellulose-rich manure through changes in microbial communities composition in intestine. This study reveals the microecological mechanisms underlying the high-efficiency conversion of cellulose-rich manure by BSFL and provide potential applications for the large-scale cellulose-rich wastes conversion by intestinal microbes combined with BSFL.

Effect of supplementing azolla and empty pea pod on growth performance, blood biochemical metabolites and meat quality in White Pekin ducks

Strategic dietary inclusion of low cost plant derived protein source in duck feed is critical for sustainability of the duck farm. Empty pea pod and azolla are two promising protein sources used as animal feed. The aim of this study was to evaluate the effect of supplementing dried and grounded azolla (AP) and empty pea pods (PP) on growth, blood biochemical metabolites and meat quality in white pekin ducks. A total of 90 white pekin ducklings were randomly assigned to 3 treatment groups (Control, T1 and T2) comprising of three replicated pens. Inside each replicate pen, a total of 10 ducklings were housed under standard management practices. AP and PP was supplemented as top up feed @ 5% of basal diet to experimental birds under T1 and T2 groups, respectively from day 0 to 56. At the end of the trial (56 day), 3 birds from each pen were sampled for serum and breast muscle. Results reveled that AP and PP has significant influence on body growth. The effect on growth was in the order of: T2&gt;T1&gt;Control. It was also observed that PP had hypoglycemic and hypolipidemic effect on ducks. However, AP and PP supplementation did not affect carcass traits, pH, cooking loss and Lipid Peroxidase Activity (TBA value) in duck meat.Thus, it could be concluded that supplementation of dried pea pods (PP) might be of great benefit to white pekin ducks as a source of cheap and unconventional protein.

The future of protein sources in livestock feeds: implications for sustainability and food safety

The feed-food competition for environmental and economic resources raises increasing concerns about the production and supply of protein for the global livestock sector. Risks to food-security and approaching deadlines for global sustainable development, means exploring alternative protein feed ingredients is imperative. This Review discusses the potential for soilless, local and circular protein feed sources to provide solutions for key sustainability and food-security threats to the global livestock sector, through their partial incorporation in future livestock feeds and feeding systems. In doing so, it offers a holistic insight into the potential opportunities, but also risks associated with such alternatives. Through this analysis, a four-point strategic plan is synthesized to facilitate higher-level policy making that may enable implementation of these alternative ingredients at commercial scales, building toward a more sustainable and resilient livestock industry.

Amino Acid Digestibility of Different Formulations of Torula Yeast in an In Vitro Porcine Gastrointestinal Digestion Model and Their Protective Effects on Barrier Function and Inflammation in a Caco-2/THP1Co-Culture Model.

Single-cell protein from torula yeast (Cyberlindnera jadinii) grown on lignocellulosic biomass has been proven to be an excellent alternative protein source for animal feed. This study aimed to evaluate the amino acid (AA) digestibility by estimating intestinal absorption from three yeast-based ingredients, produced by cultivating C. jadinii on hydrolysate, using either mixed woody species (drum- (WDI) or spray-dried (WSI)) or corn dextrose (drum-dried (DDI)) as the carbon source. Further, the protective effect of intestinal digests on activated THP1-Blue™-induced epithelial damage and cytokine profile was evaluated. Total protein content from these three ingredients ranged from 34 to 45%, while the AA dialysis showed an estimated bioaccessibility between 41 and 58%, indicating good digestibility of all test products. A protective effect against epithelial-induced damage was observed for two of the three tested products. Torula yeast cultivated on wood and drum-dried (WDI) and torula yeast cultivated on wood and spray-dried (WSI) significantly increased transepithelial electrical resistance (TEER) values (111-147%, p < 0.05), recovering the epithelial barrier from the inflammation-induced damage in a dose-dependent manner. Further, WSI digests significantly reduced IL8 (250.8 ± 28.1 ng/mL), IL6 (237.9 ± 1.8 pg/mL) and TNF (2797.9 ± 216.3 pg/mL) compared to the blank control (IL8 = 485.7 ± 74.4 ng/mL, IL6 = 478.7 ± 58.9 pg/mL; TNF = 4273.5 ± 20.9 pg/mL) (p < 0.05). These results align with previous in vivo studies, supporting torula yeast-based ingredients as a high-quality protein source for pigs, protecting the intestinal barrier from inflammatory damage, and reducing the pro-inflammatory response. We provided novel insights into the mechanisms behind the health improvement of pigs fed on torula yeast-based ingredients, with potential applications for designing nutritional interventions to recover intestinal homeostasis during critical production periods, such as weaning.

Application of Rice Field Snails (Pila ampullacea) Extract as an Alternative Substitute for Protein Sources in Export-Quality Catfish Feed (Clarias sp.)

The freshwater fishery commodity that ranks at the top in total production in Indonesia, and its high domestic consumption, is catfish (Clarias sp.). One of the efforts to provide alternative feeds is by using rice field snails (Pila ampullacea) due to their high protein content. This study aims to determine the content of P. ampullacea feed and its application as an alternative protein source in export-quality Clarias sp. The feed was prepared by combining P.ampullacea flour, rice bran, and corn flour in a ratio of 3:1:1. Analysis of water content was conducted using the weight reduction method, ash content using the ashing method, protein content using the Kjeldahl method, and fat content using the soxhletation method. The analysis results showed that P. ampullacea had water content of 7.97%, ash content of 10.36%, protein content of 50.17%, and fat content of 4.16%. Meanwhile, the P.ampullacea feed had water content of 10.49%, ash content of 8.26%, protein content of 48.74%, and fat content of 3.6%. The results indicated that the application of P.ampullacea to Clarias sp. led to an increase in protein and fat content every week, the levels found in the commercial feed used.

Efficiency of rearing Tenebrio molitor and Zophobas morio larvae as a source of feed protein

The purpose of the work was to study the effectiveness of rearing larvae of Tenebrio molitor and Zophobas morio as a source of feed protein, as well as their chemical composition and amino acid characteristics, while improving the nutrient medium. It was found that the cultivation of Zophobas morio larvae for the production of feed protein is more eff ective than Tenebrio molitor larvae. At the age of 71 days, the average weight of Zophobas morio reached 387,0±8,24 mg, which was 238,8 mg more than that of the large Tenebrio molitor or 2,6 times (p &lt; 0,001). There was a higher content of crude fat and crude protein in the chemical composition of Zophobas morio larvae by 9,4 g/kg or 6,2 % and 8,6 g/kg or 4,2 % with the same content of chitin 25–26 g/kg, calcium 0,32–0,35 g/kg and ash 23,3–24,7 g/kg. In the larvae of Zophobas morio a signifi cantly higher content of lysine was found by 9,7 g/kg or 2,3 times, valine by 29,8 g/kg or 2,5 times, leucine+isoleucine by 33,3 g/kg or 2,6 times, serine by 8,2 g/kg or 2,4 times, alanine by 13,4 g/kg or 2,3 times, glycine by 9,0 mg/kg or 2,6 times, proline by 5,9 g/kg or 2,4 times and tyrosine by 1,1 g/kg or 39,3 %. The introduction of dried daphnia into the traditional nutrient medium of Zophobas morio made it possible to signifi cantly increase the mass of larvae by 54,4 mg or 16,4 % (p &lt; 0,01), as well as the content in them such nutrient as dry matter by 13,3 g or 3,4 %, crude protein and fat by 35,3 g/kg or 19,7 % and 8,7 g/kg or 5,7 %, metabolic energy by 1,8 MJ/kg or 25,3 %, lysine by 7,1 g/kg or 68,9 %, cystine by 2,2 g/kg or 2,2 times, arginine by 16,3 g/kg or 2,5 times, leucine+isoleucine by 29,5 g/kg or 2,2 times. Such use of dried daphnia did not have a high positive effect on the growth rate of Tenebrio molitor larvae, their chemical composition and amino acid profile.

High yielding cows metabolism peculiarities under climate change conditions with the use of feed additive with protein protected from rumen degradation

The research relevance is determined by the problem of thermal load on the body of dairy cows by increasing the content of protein protected from breakdown in the rumen. The research aims to determine the metabolism and level of delivery of various forms of protein and energy to the body of highly productive cows in the diets of cows in the second half of lactation under conditions of climate change. Research methods include zootechnical (assessment of feed intake, rationing, selection of groups, organisation of the experiment, determination of milk production), biochemical (determination of the content of basic nutrients in feed, milk quality indicators), statistical, and mathematical. The compensation of protein in the diet of high-yielding cows under climate change conditions was carried out using the protein feed additive TEP-mix with protein protected from rumen breakdown. It has been proven that its inclusion in the cow’s diet can increase average daily milk production by 9.1% in the cold season (0-10°C) and by 28.0% in the summer (21-36.4°C). In addition, the fat and protein content in milk increased in cows receiving the high-protein feed additive TEP mix. It was found that the increase in protein nutrition of cows from 23.18% to 33.53% with the use of the feed additive TEP-mix with protein protected from scar breakdown ensures a reduction in the impact of climate change on the metabolism of dairy cows. Thus, it has been proved that the use of specific protein feed sources that regulate the level of non-cutting protein in the diet can be considered a factor in combating heat stress and increasing milk production of dairy cows throughout the physiological cycle of lactation, including in extreme conditions of high temperatures