This study was conducted to identify polymorphism in Booroola gene (FecB) in five Iraqi sheep breeds (Hamdani, Karadi, Arabi, Naeimi and Awassi). About fifty mature ewes (Ten for each breed used as pooled samples) were genotype for the Booroola (BMPRIB) receptor. The polymerase chain reaction (PCR) was used for amplification of a fragment with 140 bp at this locus. For genotyping of individuals at Booroola locus, the resulted amplified fragments were digested using AvaII restriction enzyme. AvaII restriction enzyme was used to detect possible mutation (G|GACC). The results showed no differences in the band patterns of digested products only the wild type alleles (Fec++) were detected and all breeds for this locus were monomorphism. Considering the phenotypic records in these breeds, the result revealed that the genetic factor responsible for litter size is not related to report of Booroola major gene and research should continue to search for other major genes ( such as FecH, FecX and FecG) in these sheep breeds.