Because of its large specific surface area, small particle size, high surface energy, and unique nanoeffect, the morphological characteristics of nanoparticles are the key factors affecting the properties of materials. How to detect and evaluate the morphological characteristics of nanoparticles is the first problem to be solved in the preparation and application of nanomaterials. The main purpose of this paper is to use TEM to recognize the image features of nanoparticles and introduce the transmission electron microscope and image edge segmentation method and random forest algorithm. A method integrating the in situ characterization of modern electron microscopy and the measurement of the electrical properties of nanomonomers was developed. In this paper, a multielectrode TEM in situ electrical measurement platform is prepared, which improves the contact during the integration of nanomaterials and improves the electrical measurement accuracy of the TEM in situ electrical method. In this paper, based on the random forest algorithm, a multirandom forest algorithm is proposed. Due to the different gray levels of images referenced by the multirandom forest algorithm, the segmentation results are processed by FCM clustering algorithm. Experimental results show that in terms of image segmentation accuracy, the minimum Jaccard coefficient obtained by multiple random forest algorithm is 89% and 95%, respectively, which is obviously better than watershed segmentation method and maximum entropy threshold segmentation. In the aspect of automatic image segmentation of nanoparticles, the image segmentation accuracy is the highest when the sample block size and the number of sample blocks selected in the multiple random forest algorithm are 5∗5, 7500, and 35, respectively. Therefore, the multirandom forest algorithm has achieved high accuracy in image segmentation of nanoparticles, which provides valuable information for the preparation and application of nanomaterials. A new type of TEM dark‐field imaging diaphragm was prepared, which greatly improved the imaging quality of weak‐phase bulk materials represented by graphene and nonspiral biological samples represented by intracellular polyvesicles.
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