Fat Accretion Research Articles

142 Influence of Comfort on Growth, Intramuscular Fat Accretion, and Ultrasound Ribeye Area in Akaushi Steers and Heifers at Time of Weaning

Abstract Comfort (Ralco Agriculture; Marshall, MN) is a feed additive that contains essential oils and capsicum which are antioxidants and selectively antimicrobial. The objective of this study was to evaluate the influence of Comfort on growth, intramuscular fat (IMF) accretion, and ultrasound ribeye area (REAU) in Akaushi and Akaushi crossbred steers and heifers at time of weaning. Steers and heifers (body weight = 257 ± 26.8 kg; n = 56) were weaned on d 0 at the ranch where they were born (Flatonia, TX). On d 0 animals were weighed, ultrasound scanned, and allocated to treatment. Treatments were a negative control that received no Comfort and a Comfort treatment, received 0.23 kg∙animal-1∙day-1 top-dressed on the ration. An equal number of steers and heifers were allocated to each treatment. Average daily high temperature was 38º C. On d 50, animals were shipped to the West Texas A&M University research feedyard (Canyon, TX); weighed and ultrasound scanned upon arrival. Data reported are the difference between d 0 and d 50 measurements. Data were analyzed using treatment as the fixed effect and individual animal as the experimental unit. Statistical analysis used PROC MIXED in SAS 9.4. Animal final BW was not different between treatments (285 COMF vs. 282 kg control; P = 0.77). Animal average daily gain (ADG) tended to be greater in the Comfort cattle compared with the control (0.61 vs. 0.47 kg/d; P = 0.09). Percentage IMF was significantly different between Comfort and control animals (0.14 vs. -0.12 %; P = 0.02). Animal REAU was not different between the treatments (3.39 for Comfort vs. 3.73 cm2 for control; P = 0.83). Fat thickness was not different between Comfort (0.1397 cm) and control cattle (0.1497 cm; P = 0.74). Data collected in this trial indicate that Comfort increased ADG and fat percentage with minimal changes to fat thickness and REAU.

Effect of the obese phenotype on brain composition in congenic lean and obese LA/Ntul//-cp rats

To determine if the obese phenotype impacted on brain development and composition, groups of congenic male lean and obese littermates of the LA/Ntul//-cp rat strain were fed on a standardized nutritionally adequate diet in littermate pairs from weaning until adulthood. The obese phenotype of his strain develops early onset chronic hyperinsulinemia associated with hypertrophic-hyperplastic obesity during early postweaning growth. At 10.5 months of age, animals were sacrificed by cervical dislocation, and brain tissues excised in its entirety, weighed to the nearest mg, and measures of protein, DNA and lipid determined. Body weights (BW) of obese were significantly greater than lean. Brain mass (BrM) of lean > obese, and BrM:BW of lean was greater obese. Brain protein content (BPC) and Brain DNA (BDNA) of lean was greater than obese. Brain lipid as a percent was similar in both phenotypes and net brain lipid content was proportional to brain mass. Total body fat mass of obese was significantly greater than occurred in lean littermates. Inflammatory cytokines residing in adipose tissue have been reported to contribute to DNA damage in neuronal and other tissues, impede cell replication, and accelerate cell senescence. These results indicate that brain growth and cellular development is impaired in the hyperinsulinemia-prone obese phenotype of this strain, and are likely associated with development of a chronic inflammatory syndrome and cytokine expression common to excessive fat accretion and obesity

Effect of the obese phenotype on expression of hepatic T4–5’ deiodinase activity, T3 generation and thyroidal mediated actions in congenic LA/Ntul//–cprats

Parameters of thyroidal responses to diet and environment have long been shown to be impaired in the obese phenotype of the LA/Ntul//–cp rat. To characterize the effect of expression of the obese phenotype on activity of maximum T4–5’ deiodinase (T4–DI) activity on adiposity and aspects of metabolism, groups of lean and obese littermates were subjected to measures of resting metabolic rate (RMR, VO2) at thermal neutrality (30°C) after a brief fast, followed by dose response Norepinephrine (NE) stimulation. Hepatic T4–DI activity represents a major source of hormonally active T3 in plasma and is readily delivered to peripheral tissues. At 16 weeks of age animals were sacrificed by cervical dislocation, blood was collected, and liver tissue was dissected for measures of serum levels of T3 and T4, and measures of maximal tissue dithiolthreitol (DTT)–stimulated T4–DI activity determined. The retroperitoneal and dorsal white adipose (WAT) depots were also dissected to estimate the magnitude of adiposity present upon sacrifice in each phenotype. Measures of RMR and the VO2 response to NE were greater in lean than obese phenotype, and indicators of BW and adiposity were much greater in obese than lean (p=<0.01). Measures of serum T3 were greater in lean than obese, but T4 was similar in both phenotypes. Measures of the rate of hepatic T4 DI activity were greater in lean than obese (p=<0.05), and the T1/2 for T4 disappearance were also prolonged in the obese phenotype. These results are consistent with other studies of impaired nonshivering thermogenesis in this strain, and suggest that decreased activity of hepatic T4–5’ deiodinase activity may be a contributing factor in the impaired capacity for nonshivering thermogenesis and excess fat accretion in the obese phenotype of this strain.

Dietary Oil Type and Late Feed Restriction Elicit Synergistic Effects on Growth, Caeca Bacteria, Carcass, Fat Accretion, and Muscle Lipids in Female Broilers

AbstractThe influence of dietary oil type (OT) and late feed restriction (FR) on growth, caeca bacteria count, carcass, separable fat depots, serum and muscle lipids, and meat quality in broilers is examined. In total, 224, 21‐day‐old female Arbor acres broilers are randomly allotted to either PN, Unrestricted+50 g kg−1 palm oil (PO); PR, 25%‐FR+PO; SN, Unrestricted+50 g kg−1 soybean oil (SO); or SR, 25%‐FR+SO for 21 d. The SN and PR birds have the heaviest and lightest body weight gain (BWG), respectively. Feed conversion ratio (FCR) is poorer in the PN birds. Feed‐restricted birds have more caeca Lactobacillus and lower Salmonella, serum cholesterol, and triglycerides. Carcass yield is higher in SR birds and lower in PN birds. The FR‐OT interaction is significant for muscle lipids and weight of fat depots. The PN birds deposit more abdominal, intramuscular, sartorial, mesentery and neck fats, muscle saturated fatty acids, and C18:1n‐9 and lower C18:3n‐3 and C20:5n‐3 than other birds. Neither OT nor FR affects the pH, color, water holding capacity, and oxidative stability of breast meat. The changes elicited by 25%‐FR on BWG, FCR, carcass yield, depot fats, and muscle lipids in female broilers are dependent on dietary OT.Practical Application: Fast growth rate and unrestricted access to feed can predispose broiler chickens to excessive fat accretion, which is counterproductive. Producing high‐quality carcasses that meet consumers’ expectation is crucial for sustainable and efficient broiler production. The possible interaction between dietary OT and late quantitative FR in mitigating excessive fat accretion and altering production indices in female broilers is explored. Late quantitative FR reduces BWG, FCR, serum lipids, caeca pH and Salmonella spp. and fat accretion in fat depots, and enhances carcass yield and caeca Lactobacillus spp. However, the impacts are more pronounced in the PO birds than the SO birds. Late FR improves the C18:3n‐3 and C18:2n‐6 contents in PO meat while reducing the C14:0 and C16:0 contents in SO meat. These findings explicate that dietary OT and late quantitative FR can be synergistically used to mitigate excessive fat accretion and alter growth, caeca microflora, and muscle lipids in broilers.

Fat Accretion and Growth in the Rat

The normal course of fat accretion in male rats from birth to 3 years is described. The relative amount of fat and relative size of fat pads increased steadily throughout life (except for a brief cessation in fat accretion at weaning), in a manner closely related to body weight and independent of age. Several new rat strains with extremes of growth or fat accretion, fed the same diet, were used to define and illustrate courses of fat accretion: very large and very small non-obese strains and a hereditarily obese rat. Rats of different known strains fed a variety of more refined diets show a course of fat growth different from that obtained with stock diets. Several types of obesity are discussed: insulin obesity, nutritional obesity, and hereditary obesity. Hereditary size and growth of the rat were readily (in a few generations) altered up or down, by consistently using the largest or the smallest 25% of the population for breeders. It is probable that the large increase in size of some laboratory rats over the past 40 years has been accomplished not only by improvement in stock diets and colony conditions, but also by selection for increased hereditary size.

Phosphatides and Cholesterol in the Rat Body: Effects of Growth, Diet and Age

Data are presented on the content of phosphatides, free and total cholesterol, and neutral fat in 152 male rats from birth to 3 years of age. The ratio of phosphatides to total cholesterol remained remarkably constant at near 4 (mol ratio of 2) for the first year of life with a variety of diets. Two- and three-year-old rats showed a highly significant decrease in this ratio, corresponding to an increase in total cholesterol of about 10%, with no change in phosphatides. The percentage of free cholesterol decreased steadily throughout life from 90 to 72, diet and senescence having no effect thereon. The pattern of accretion of neutral fat with stock diet was in many respects very different from the common pattern observed for both phosphatides and cholesterol. The substitution of more concentrated, refined or purified diets of varying fat content (2% to approximately 40%) for the crude stock diet led to no change in phosphatides or cholesterol. These same rats showed large increases in neutral fat.

Abstract MP12: Dash Diet and Its Components is Associated With Visceral Fat Area and Hepatic Attenuation Among South Asian Adults in the Mediators of Atherosclerosis in South Asians Living in America (MASALA) Study (2010-2013)

Introduction: South Asians (SA) are a growing population group in the US that remain understudied in research despite their high cardiometabolic risk. Compared to other ethnic groups, SA experience cardiometabolic disease onset at a younger age, and have higher abdominal, visceral and hepatic fat despite lower BMI. SA in the US have a unique dietary pattern characterized by a combination of traditional and western food items. Examining the dietary pattern of SA in relation to central adiposity may provide a tenable strategy for disease prevention and treatment. Hypothesis: We assessed the hypothesis that higher DASH diet score is associated with lower central adiposity, indicated by visceral fat area and hepatic attenuation measurements, among SA in the US. Methods: Using baseline data from 822 adults enrolled in the Mediators of Atherosclerosis in South Asians Living in America (MASALA) study, we computed a Dietary Approaches to Stop Hypertension (DASH) concordance score from the FFQ validated to measure diet of South Asians in North America. Using an established method, score concordance was based on 8 components including high intake of fruit, vegetables, nuts/legumes, low-fat dairy products, whole grains; and low intake of sodium, sugar-sweetened beverages, and red/processed meats. We examined the association between the DASH score and its components with CT imaging of visceral fat area and hepatic attenuation (higher score indicates lower liver fat). Results: The overall DASH score among participants ranged from 13-35 (mean ± SD, 24.6 ± 4.4). Hepatic attenuation was 5 HU higher among participants in the highest category DASH score compared to the lowest (aβ (SE): 5 (1), 95% CI: 2, 7) in the age-adjusted model. The relationship was not significant in the fully adjusted model. Visceral fat area was 13.6 cm 2 lower among participants in the highest category of DASH score compared to the lowest (aβ (SE): -3.6 (5.1), 95% CI: -23.5, -3.7) in the model adjusted for age, sex, percent of life in US, education, BMI, physical activity, smoking, alcohol, and energy intake. When examining individual components of DASH, participants with the highest intake of low-fat dairy had hepatic attenuation 3 HU higher than those with the lowest intake, and those with the lowest intake of red/processed meat had hepatic attenuation 2 HU higher in the fully adjusted model. Participants with the lowest red/processed meat intake had visceral fat area that was 11 cm 2 lower in the fully adjusted model. Conclusions: In conclusion, our results indicate that greater adherence to DASH and some of its individual components is associated with favorable abdominal fat areas among a sample of SA adults in the US. These findings support the relationship between diet and abdominal fat accretion. Forthcoming analyses will use the DASH score to further elucidate the diet-disease relationship among SA in the US.

Nonshivering thermogenesis revisited: sympathetic and non-sympathetic contributions

Factors of diet and environment contribute to complementary, additive mechanisms that influence the magnitude of diet induced thermogenesis generated in man and animals. Both short terms sympathetically mediated and longer acting thyroidal mediated pathways have been identified and appear to function in a metabolically coordinated fashion to facilitate biochemical and physiologic pathways of energy balance implicating multiple organs and peripheral tissues during periods of over- and under-nutrition. In a congenic lean rodent model known to express parameters of environmentally and diet induced mediated thermogenesis, thyroidal and sympathetic contributions each contributed to approximately 50% of the relative increase in VO2 in quietly resting animals after consuming a high energy palatable diet as determined in the presence vs. absence of α-methylparatyrosine, a sympathoplegic chemical agent. In contrast, in the obese phenotype of the corpulent and other rodent strains, both sympathetic and thyroidal contributions to adaptive thermogenic mechanisms are impaired in response to both nutritional and environmental challenge, contributing to a greater efficiency of energy metabolism and body fat accretion. Thus, the purpose of the present review is to approximate the qualitative distribution between the two primary factors linked to the physiologic process resulting in the expression of diet induced thermogenesis in a normally lean phenotype of rat, the LA/Ntul//-cp.

Resistant Starch Decreases Serum Total Cholesterol and Triacylglycerol Concentrations in Rats

Rats were meal-fed semipurified diets containing a low (0.8 g/MJ) and a high (9.6 g/MJ) amount of resistant starch (RS) or various amounts of RS (0.8 to 9.6 g/MJ) and guar gum (0 to 8.8 g/MJ). In one experiment, rats were fed the low and high RS diets in three dietary regimens (ad libitum consuming, 12 h ad libitum/12 h food deprived, and meal fed). Effects of RS and guar gum on serum postprandial and postabsorptive concentrations of total cholesterol (TC) and triacylglycerol (TAG), growth, hydrogen excretion, tissue weights and contents of small intestine and cecum, and pH of cecal contents were investigated. In addition, effects of RS on food intake, de novo hepatic synthesis of fatty acids and neutral sterols, and on lipoprotein lipase activity and weight of epididymal fat pads were investigated. Compared with feeding the low RS diet, the high RS diet reduced the serum TC and TAG concentrations, with these effects observed after 1 and 2 wk of feeding, respectively. The dietary regimen did not influence the effect of RS on the serum TC and TAG concentrations, but it did affect the serum TAG concentration. Resistant starch had no effect on the hepatic synthesis of fatty acids and neutral sterols or on the lipoprotein lipase activity in epididymal fat pads. Guar gum also reduced the serum TC concentration, but it had no effect on serum TAG concentration. The tissue weights and contents of small intestine and cecum as well as hydrogen excretion increased with increasing amounts of dietary RS and guar gum, whereas the pH of cecal contents decreased. No effects of RS on food intake and total body weight gain were found, whereas guar gum decreased weight gain. Feeding the high RS diet also led to a lower weight of the epididymal fat pads. We conclude that dietary RS can reduce serum TC and TAG concentrations and fat accretion.

Does Carbohydrate and Insulin Resistance of Obesity Contribute to the Senescence of Brain Atrophy in Aging? Here is What the Lab Rats May Be Telling Us about Carbohydrates

Since the dietary guidelines were adjusted several decades ago in an effort to improve cardiovascular health by promoting diets containing fewer calories from fats, the consumption of carbohydrate calories including those from refined carbohydrate sources have increased proportionately in much of industrialized society. Refined carbohydrates including sucrose are rapidly digested by brush border glucosidases and have a higher glycemic index and stimulate greater insulinogenic responses than calories obtained from complex carbohydrate sources and tend to contribute to greater body fat accretion over time. High carbohydrate diets promote greater elevations in insulin secretion and can lead to insulin resistance and its multiple pathophysiologic sequelae including increases in body fat accretion, obesity, non-insulin dependent diabetes (NIDDM) chronic inflammation, and other comorbidities that can compromise health and longevity including neurologic demise. In the obese phenotype of the aging congenic, chronically insulin resistant non- diabetic LA/Ntul//-cp rat, measures of brain composition and cellularity as determined by brain mass and DNA content were significantly decreased and were further compromised when fed a high glycemic index, insulinogenic diet where the starch was replaced with isocaloric quantities of sucrose. These observations are consistent with the chronic insulin and amylin resistance linked to high carbohydrate diets in the obese phenotype as a contributing factor in the progression of senescence and brain atrophy and suggest that the factors of obesity and insulin resistance, compounded by the glycemic index of the diet were key factors in the neurologic demise and premature death in the obese phenotype of this strain.

Effects of maternal dietary fatty acids during mid-gestation on growth, glucose metabolism, carcass characteristics, and meat quality of lamb progeny that were fed differing levels of dry matter of intake

This experiment evaluated growth, glucose metabolism, carcass characteristics, and meat quality of market lambs that were offered ad libitum or restricted (85% of ad libitum) feed intake following two different maternal fatty acid (FA) supplementations while in-utero. Ewes received either a diet supplemented with polyunsaturated FA or saturated/monounsaturated FA during mid- to late-gestation. Following weaning, progeny wethers were fed either ad libitum or a restricted level of feed intake. Ewe FA supplementation did not affect (P ≥ 0.11) growth, meat quality, nor plasma glucose or insulin concentrations of the progeny. Carcass body fat and yield grade of the progeny were affected (P = 0.01) by maternal FA supplementation and restricted feed intake. In summary, maternal FA supplementation did not affect progeny growth, while feed restriction during finishing did not affect meat quality. The interaction between maternal FA supplementation and finishing strategy for body fat accretion indicates that metabolism and the supply of FA during gestation may warrant further investigation.

Metabolic imprinting induced by a high-sugar diet: effects on microRNA expression and insulin resistance in young rats.

Metabolic imprinting describes associations between nutritional experiences of early life and the development of diseases later in life. The goal of this study was to evaluate the metabolic imprinting induced by a high-sugar diet (HSD) and its effects on microRNA (miRNA) expression and insulin resistance (IR) in young rats. We assessed the effects of expression of adipogenic (miR-200c) and metabolic (miR-126a) miRNAs in retroperitoneal white adipose tissue (rWAT) on IR development. Weaned male Wistar rats (N = 6) were fed a standard chow diet or HSD (68% carbohydrates) for 4-, 8-, or 12-weeks. Serum samples were collected to measure triacylglycerol and VLDL-cholesterol, and we assessed glucometabolic parameters (glucose, insulin, HOMA-IR, and QUICKI). rWAT was collected for microRNA analysis (N = 3). The HSD resulted in body fat accretion and IR after 8-weeks, which resolved by 12-weeks. Moreover, the HSD had a time-dependent effect on miRNA relative expression, downregulating rno-miR-200c-3p at week 8 and rno-miR-126a-3p at week 12. MiR-200 family dysregulation has been related to IR, and miR-126a downregulation could be associated with the improvement in IR observed after a 12-week HSD feeding period. This is the first time that excessive sugar intake post-weaning has been associated with miRNA production by rWAT with an impact on IR development.

Maternal diabetes independent of BMI is associated with altered accretion of adipose tissue in large for gestational age fetuses.

AimTo analyse the effects of maternal diabetes mellitus (DM) and body mass Index (BMI) on central and peripheral fat accretion of large for gestational age (LGA) offspring.MethodsThis retrospective study included LGA fetuses (n = 595) with ultrasound scans at early (19.23 ± 0.68 weeks), mid (28.98 ± 1.62 weeks) and late (36.20 ± 1.59 weeks) stages of adipogenesis and measured abdominal (AFT) and mid-thigh (TFT) fat as surrogates for central and peripheral adiposity. Women were categorised according to BMI and DM status [pre-gestational (P-DM; n = 59), insulin managed (I-GDM; n = 132) and diet managed gestational diabetes (D-GDM; n = 29)]. Analysis of variance and linear regressions were applied.ResultsAFT and TFT did not differ significantly between BMI categories (normal, overweight and obese). In contrast, AFT was significantly higher in pregnancies affected by D-GDM compared to non-DM pregnancies from mid stage (0.44 mm difference, p = 0.002) and for all DM categories in late stage of adipogenesis (≥ 0.49 mm difference, p < 0.008). Late stage TFT accretion was higher than controls for P-DM and I-GDM but not for D-GDM (0.67 mm difference, p < 0.001; 0.49 mm difference, p = 0.001, 0.56 mm difference, p = 0.22 respectively). In comparison to the early non-DM group with an AFT to TFT ratio of 1.07, the I-GDM group ratio was 1.25 (p < 0.001), which normalised by 28 weeks becoming similar to control ratios.ConclusionsDM, independent of BMI, was associated with higher abdominal and mid-thigh fat accretion in fetuses. Use of insulin improved central to peripheral fat ratios in fetuses of GDM mothers.

Dietary Flavonoids as Modulators of Lipid Metabolism in Poultry

Flavonoids, naturally-occurring compounds with multiple phenolic structures, are the most widely distributed phytochemicals in the plant kingdom, and are mainly found in vegetables, fruits, grains, roots, herbs, and tea and red wine products. Flavonoids have health-promoting effects and are indispensable compounds in nutritional and pharmaceutical (i.e., nutraceutical) applications. Among the demonstrated bioactive effects of flavonoids are anti-oxidant, anti-inflammatory, and anti-microbial in a range of research models. Through dietary formulation strategies, numerous flavonoids provide the ability to support bird health while improving the nutritional quality of poultry meat and eggs by changing the profile of fatty acids and reducing cholesterol content. A number of such compounds have been shown to inhibit adipogenesis, and promote lipolysis and apoptosis in adipose tissue cells, and thereby have the potential to affect fat accretion in poultry at various ages and stages of production. Antioxidant and anti-inflammatory properties contribute to animal health by preventing free radical damage in tissues and ameliorating inflammation in adipose tissue, which are concerns in broiler breeders and laying hens. In this review, we summarize the progress in understanding the effects of dietary flavonoids on lipid metabolism and fat deposition in poultry, and discuss the associated physiological mechanisms.

Molecular Liver Fingerprint Reflects the Seasonal Physiology of the Grey Mouse Lemur (Microcebus murinus) during Winter.

Grey mouse lemurs (Microcebus murinus) are primates that respond to environmental energetic constraints through strong physiological seasonality. They notably fatten during early winter (EW), and mobilize their lipid reserves while developing glucose intolerance during late winter (LW), when food availability is low. To decipher how the hepatic mechanisms may support such metabolic flexibility, we analyzed the liver proteome of adult captive male mouse lemurs, whose seasonal regulations are comparable to their wild counterparts. We highlight profound hepatic changes that reflect fat accretion in EW at the whole-body level, without triggering an ectopic storage of fat in the liver, however. Moreover, molecular regulations are consistent with the decrease in liver glucose utilization in LW, and therefore with reduced tolerance to glucose. However, no major regulation was seen in insulin signaling/resistance pathways. Fat mobilization in LW appeared possibly linked to the reactivation of the reproductive system while enhanced liver detoxification may reflect an anticipation to return to summer levels of food intake. Overall, these results show that the physiology of mouse lemurs during winter relies on solid molecular foundations in liver processes to adapt fuel partitioning while opposing the development of a pathological state despite large lipid fluxes.

α-Linolenic acid rich-chia seed modulates visceral adipose tissue collagen deposition, lipolytic enzymes expression, insulin signaling and GLUT-4 levels in a diet-induced adiposity rodent model

Given obesity and its associated metabolic disorders have reached epidemic proportions, the study of therapeutic strategies targeting white adipose tissue (WAT) are of main research interest. We previously shown that α-linolenic acid–rich chia seed was able to ameliorate a wide range of metabolic disorders including body fat accretion in sucrose-rich diet (SRD)-fed rats, an experimental model of visceral adiposity and insulin resistance. However, the mechanisms involved are not fully clarified. The aim of this study was to evaluate the effect of chia seed administration upon WAT remodeling and key enzymes that controls lipolysis, insulin signaling (tAKT, pAKT), and GLUT-4 levels in different visceral fat pad depots (epididymal –eWAT- and retroperitoneal –rWAT- adipose tissues) of SRD-fed rats. Results showed that chia seed reduces adipocytes hypertrophy, the increased lipid content and collagen deposition in both WAT. These changes were accompanied by a significant reduction of HSL and ATGL protein levels in eWAT and HSL protein levels in rWAT. Moreover, chia seed restored the altered expression pattern of the pAKT observed in SRD-fed rats, and modulated GLUT-4 levels. Chia seed could be a dietary intervention of great relevance with potential beneficial effects in the management of body fat excess and WAT function.

Abstract 59: Prenatal predictive factors and prevalence of early postpartum maternal dysglycemia in patients with gestational diabetes

Background: Women with gestational diabetes mellitus (GDM) have increased risk of postpartum diabetes which may be further exacerbated by fat accretion during pregnancy and post-partum deterioration in lifestyle behaviours.Aims and objectives: To determine prenatal predictive factors and prevalence of maternal dysglycemia at 6-12weeks postpartum in patients with GDM.Results: In this prospective cohort study, of 118 subjects recruited initially, only 83 (81.3%) followed for postpartum screening. Among them 38.56%, 22.89% and 8.43% had family history of Diabetes Mellitus, Previous history of GDM and PCOD respectively. We observed that 62.7% reverted to NGT postpartum, however; 8.4%, 3.6%, 10.8% and14.5% had IFG, IGT, IFG+IGT and overt DM respectively.In univariate analysis, BMI (p=0.001), family history of DM (p=0.008), PCOD (p=0.012), impaired fasting glucose (p<0.001), HbA1c at diagnosis (p<0.001) and fetal macrosomia (p=0.019) were the risk factors for postpartum diabetes. However on Multivariate analyses, only HbA1c at diagnosis, Family history of DM and BMI showed statistically significant association with postpartum diabetes.Conclusion: GDM patients have a high prevalence of postpartum glucose intolerance and diabetes. The prenatal risk factors identified could be useful for prenatal risk stratification for early detection and interventions to reduce the risk of progression and complications of diabetes mel.

Body composition from birth to 2 years in term healthy Indian infants measured by deuterium dilution: Effect of being born small for gestational age and early catch-up growth.

South Asian body composition is characterized by higher body fat at any given BMI. While this does not occur during fetal growth, it is important to understand if inappropriate fat accretion then begins in the first 2 years in Indian infants. The fat mass (FM) and fat-free mass (FFM) of healthy term newborns was evaluated at 12 days, 3.5 months, 1 year, and 2 years, by deuterium oxide (D2O) dilution. The effect of being born small versus appropriate for gestational age (SGA vs. AGA), and accelerated early growth pattern on FM and FFM accretion was also investigated. Newborns (262 total, 150 males) with mean birth weight of 2863 ± 418 g were enrolled. FM percentage (FM%) assessed by D2O in 144, 166, 81, and 115 infants at 12 days, 3.5 months, 1 year, and 2 years respectively, was11.6 ± 6.8, 21.1 ± 7.0, 17.9 ± 8.2 and 22.4 ± 9.5%. Boys had higher FFM at all ages, but FM% was similar in both sexes. Children born SGA had similar FM index (FMI) but a lower FFM index (FFMI) at 2 years compared with those born AGA. Infants with catch-up growth between 0 and 2 years had higher FMI at 2 years compared to those without. Infants in the present study had a lower FM% and FMI till 1 year of age in comparison to previous studies from other countries, but had an increase in adiposity between 1 and 2 years, whereas in previous studies FM% remained stable or declined between 1 and 2 years of age. There was an upward inflection in the curve of FM% and FMI between 1 and 2 years of age in the present study, which may represent an early adiposity rebound. Further longitudinal body composition data for Indian infants as well as those of other ethnicities but with low birth weight will clarify whether early accelerated growth pattern contributes to greater accrual of fat rather than lean mass during childhood.

Anthropometry Based Growth and Body Composition in Infants with Complex Congenital Heart Disease.

Infants with congenital heart disease (CHD) often have poor growth and altered body composition (fat and muscle accretion). Describe growth patterns in infants with CHD using interval weight, length, head circumference (HC), triceps (TSF), subscapular skinfolds (SSSF), and mid-upper arm circumference (MUAC) measurements. A total of 120 infants enrolled: 48% healthy and 58% with CHD (45% single ventricle [SV]; 55% two ventricle [2V] physiology). Weight, length, HC, TSF, SSSF, and MUAC measured at 3-, 6-, 9-, and 12-months of age. CHD infants had lower weight, length, and HC z-scores at 3-, 6-, and 9-months. At 9-months, infants with SV physiology had larger TSF and SSSF z-scores over 2V and healthy infants. Overall MUAC z-scores were smaller at 3- and 6-months in infants with CHD. Infants with CHD have a complex pattern of growth. Longitudinal growth and body composition measurements provide information to better understand this pattern.

Specific adipose tissue Lbp gene knockdown prevents diet-induced body weight gain, impacting fat accretion-related gene and protein expression

Lipopolysaccharide binding protein (Lbp) has been recently identified as a relevant component of innate immunity response associated to adiposity. Here, we aimed to investigate the impact of adipose tissue Lbp on weight gain and white adipose tissue (WAT) in male and female mice fed an obesogenic diet. Specific adipose tissue Lbp gene knockdown was achieved through lentiviral particles containing shRNA-Lbp injected through surgery intervention. In males, WAT Lbp mRNA levels increased in parallel to fat accretion, and specific WAT Lbp gene knockdown led to reduced body weight gain, decreased fat accretion-related gene and protein expression, and increased inguinal WAT basal lipase activity, in parallel to lowered plasma free fatty acids, leptin, triglycerides but higher glycerol levels, resulting in slightly improved insulin action in the insulin tolerance test. In both males and females, inguinal WAT Lbp gene knockdown resulted in increased Ucp1 and Ppargc1a mRNA and Ucp1 protein levels, confirming adipose Lbp as a WAT browning repressor. In perigonadal WAT, Lbp gene knockdown also resulted in increased Ucp1 mRNA levels, but only in female mice, in which it was 500-fold increased. These data suggest specific adipose tissue Lbp gene knockdown as a possible therapeutic approach in the prevention of obesity-associated fat accretion.