Background: The main role in the diagnosis of human echinococcosis belongs to instrumental methods. Enzyme-linked immunosorbent assay (ELISA) for determinftion the specific echinococcosis IgG is important in predicting the development of relapses and evaluating the effectiveness of treatment. This method has significant deficiency – false-positive results due to cross-reactions in patients with other parasitic or allergic diseases. Purpose: To evaluate the value of ELISA and immunoblotting for diagnosis of echinococcosis and monitoring the effectiveness of treatment. Methods & Materials: We analyzed 1500 serum samples of patients with echinococcosis and persons suffered from another parasitic or allergic diseases. The study was carried out by ELISA for detection of specific Echinococcus granulosus IgG. Also 66 serum samples containing specific IgG were studied by immunoblotting. Results: ROC analysis showed that the sensitivity of ELISA in determination of specific Echinococcus granulosus IgG was 92.23%, specificity 88.87%, AUC is 0.91. The predictive value of a positive result was 56.89%, negative – 98.63%. Immunoblotting results of 66 samples containing IgG showed: in 6 patients with enterobiosis a positive result was due to non-specific P39 protein (39 kDa). In 3 patients who underwent surgical treatment due to alveococcosis P16/18 (16–18 kDa) proteins were detected, specific for all another echinococcosis except echinococcus granulosus. Specific P7 protein (7 kDa) and non-specific P39 were detected before surgery in 15 patients with isolated uncomplicated echinococcosis. Six months after surgery and antigelminthic treatment we observed 5-fold decrease in level of IgG and only non-specific P39 in 6 patients, in 9 cases it was happen only after 3 years. In 18 patients with multiple recurrent echinococcosis highly specific P7, non-specific P39 and P16/18 (16–18 kDa) proteins were detected one month after repeated surgical treatment. One year after the completion of 6-month course of anti-relapse therapy a decrease of level the specific immunogobulins was noted, and positive results in ELISA was due to non-specific P16/18. Conclusion: The use of serological methods in the diagnosis of echinococcosis and monitoring the effectiveness of surgical and anti-relapse treatment is justified. The method of immunoblotting allows to differentiate echinococcosis and detect false positive ELISA results with other parasitic invasions.