Guava Extract Research Articles

CHARACTERIZATION OF NANO PARTICLES ETHANOL EXTRACT OF GUAVA (PSIDIUM GUAJAVA) FRUIT WITH VARIATION OF CHITOSAN-ALGINATE

The ethanol extract of Guava (Psidium Guajava) fruit is known to have strong antioxidant activity so that it has the potential to be developed into a nanoparticle delivery system. The purpose of this study was to determine the effect of variations in the concentration of chitosan - alginate polymer on the physical characteristics of guava fruit extract nanoparticles (Psidium Guajava). Guava Fruit Extract was formulated in the form of nanoparticles by ionic gelation method with variations in the concentration of chitosan:alginate polymer, namely 0.5% : 0.5% (F1), 0.75% : 0.5% (F2), and 1% : 0.5% (F3). The test parameters include the percentage of transmittance. The optimal formulation is based on the level of clarity of the solution and the percentage of transmittance obtained in the formulation ratio of 0.75%: 0.5% (F2), with an average transmittance of 91.9%. Based on the results of the research that has been done, it can be concluded that guava fruit extract can be formulated in nanoparticle size with varying physical characteristics depending on the concentration of chitosan and alginate used.

Aqueous Extract of Guava (Psidium guajava L.) Leaf Ameliorates Hyperglycemia by Promoting Hepatic Glycogen Synthesis and Modulating Gut Microbiota.

Type 2 diabetes mellitus (T2DM) is a major global health concern. Psidium guajava L. (guava) is widely used for food as well as a folk medicine. Previous studies have shown its anti-diabetic and anti-inflammatory properties. However, the underlying mechanisms remains to be elusive. In this study, we assessed the potential therapeutic effects of aqueous extract of guava leaves (GvAEx) on T2DM and explored their potential mechanisms in vivo and in vitro. GvAEx was gavage administered for 12 weeks in diabetic db/db mice. Our results have demonstrated that GvAEx significantly lowered fasting plasma glucose levels (p < 0.01) and improved glucose tolerance and insulin sensitivity (p < 0.01, p < 0.05, respectively). Additionally, GvAEx increased hepatic glycogen accumulation, glucose uptake and decreased the mRNA expression levels of gluconeogenic genes. Furthermore, GvAEx-treatment caused higher glucose transporter 2 (GLUT2) expression in the membrane in hepatocytes. Notably, for the first time, we have elaborated the possible mechanism of the hypoglycemic effect of GvAEx from the perspective of intestinal microbiota. GvAEx has significantly changed the composition of microbiota and increased short chain fatty acid (SCFA) -producing Lachnospiraceae family and Akkermansia genus in the gut. Taken together, GvAEx could alleviate hyperglycemia and insulin resistance of T2DM by regulating glucose metabolism in the liver and restoring the gut microbiota. Thus, GvAEx has the potential for drug development against T2DM.

IDF21-0412 Phyto-Pharmacological Benefits of Ethanol Extract of Guava Leaves on Streptozotocin Induced Type-2 Diabetic Model Rats

IDF21-0412 Phyto-Pharmacological Benefits of Ethanol Extract of Guava Leaves on Streptozotocin Induced Type-2 Diabetic Model Rats

EVALUATION OF THE SECURITY OF THE USE OF GUAJAVA PSIDIUM EXTRACT CAPSULE ON HEART FUNCTION IN DENGUE FEVER AND DENGUE HEMORAGIC FEVER PATIENTS IN TYPE C HOSPITAL

&#x0D; There are 126,675 DHF sufferers in 34 provinces and 1,229 of them died, while the Incidence Rate (IR) in Central Java is 47.9 per 100,000 population. Based on the safety of Psidium guajava Linn on the liver by calculating the lethal dose average showed that there were no deaths from the two experimental models of Swiss mice and Wistar mice in the dose range up to 2,000 mg/kg BB. Safety tests in rats proved lethal dose 50 (LD50) of guava leaf extract more than 5 g/kg. This study aims to determine the effect of psidii capsules containing guava leaf extract (Psidium guajava Linn.) toward levels (SGOT and SGPT) of DF and DHF patients in PKU Muhammadiyah Wonosobo Hospital. This study belongs to perspective cohort analytic observational using blood plasma patients of PKU Muhammadiyah Wonosobo Hospital of 37 patients in the period of March to August 2018 which were divided into two groups, namely standard therapy + guava leaf extract capsules and the group receiving standard therapy only. Dosage of Psidium guajava 3x2 capsules for 3 to 4 days. Examination of SGOT and SGPT levels used UV-vis spectrophotometry. General linear model was used for analyzing the data. The average SGOT level after treatment was 64 U/L in DF and 99 at DHF while the SGPT levels were 30 U/L and 35 U/L. Average levels of SGOT and SGPT decreased. There were no differences in SGOT and SGPT mean of DF and DHF patients in the two groups after giving guava leaf extract capsules with p&gt;0.05. In SGOT mean, there was an increase in SGOT after giving guava leaf extract probably because there were patients who had chronic liver disorders.&#x0D; &#x0D; &#x0D; &#x0D; &#x0D; &#x0D; Keywords: Dengue Fever, Dengue Hemorrhagic Fever, Guava extract capsule, SGOT SGPT&#x0D;

Salivary Active MMP-2 of Breast Cancer Patients Is Inhibited by Guava Leaves PBS Extract

Matrixmetalloproteinase-2 (MMP-2), also called gelatinase-A, is a 72 Kd protein present in chromosome 16 in human. It is a zinc binding protein, responsible for the degradation of Extra Cellular Matrix (ECM) in normal physiological as well as disease processes like arthritis and cancer. It has a specific role in the cancer development and angiogenesis. MMP-2 contributes to cell migration. MMPs are the key to normal development as well as in the pathology of cancer and other inflammatory diseases. The inhibitors of MMP-2 activity are very important in maintaining the normal activity of MMP-2 and have an important role in the management of cancer. Regulation of MMP-2 activity is done by inhibitors like, TIMPs. In this report, we are discussing about a possible inhibitor(s) present in the PBS extract of guava leaves, inhibiting the active MMP-2 present in the saliva of breast cancer patients.

Role of Plant Volatile to Diaphorina citri on Feeding and Oviposition Behaviour

&#x0D; &#x0D; &#x0D; Citrus production in Indonesia and in the world is suffered from disease of citrus vein phloem degeneration (CVPD. It was vectored by psylids (Diaphorina citri). The psylids used citrus plant volatiles as cues for finding their host plants for feeding and laying eggs. Extract of guava leaves was a prospective control material for declaining population of psyllid. Investigation by research was conducted to determine the effect of CVPD symptomatic citrus plants in attracting psylids gravid female for staying and laying eggs, and also to determine the repellency ability of guava shoots to adult psyllids. Citrus buds of healthy plants and CVPD symptomatic plants were exposed to ten gravid female and the number of psylids stay and the number of eggs per bud was recorded. Y-tube olfactometer was used to determine repellent effect of upper shoot, middle shoot, and bottom shoot of guava leave to ten adult psyllids. The result shows that CVPD symptomatic plants was more attractive for laying eggs, eventhough the number of eggs was higher on healthy plants. Repellence effect to psyllids adult was identified in guava leave extracts. The increase of leaf age would decrease the effect. It was suggested that repellent properties was highest youngest leaf or upper shoots of guava.&#x0D; &#x0D; &#x0D;

ANTIBACTERIAL AND PHYTOCHEMICAL STUDIES OF PSIDIUM GUAJAVA LEAF EXTRACT

The phytochemical screening, and anti-bacterial activities of the hydro-methanolic leaves extract of Psidium guajava using standard screening methods such as disc diffusion methods. The secondary metabolites such as alkaloids, phenols , saponins, and tannins were present in Psidium guava extract in phytochemical screening, It also showed dose dependent antibacterial activities against almost all the test organisms. Results denote the antibacterial activity of Psidium guajava leaves extract

The Effect of Ethanol Extract of Guava (Psidium guajava L) Leaf on Hypercholesterolemia-Diabetic White Rats Male Induced by High Fat Feed and Streptozotocin

This study aims to determine the effect of guava (Psidiium guajava L) leaf extract on hypercholesterolemia-diabetic white rats male and the effective dose for reducing cholesterol and blood glucose levels. This study is a laboratory experimental study using 30 rats divided into 6 test animals. the treatment group, each group consisted of 5 rats. Group I was normal control, group II negative control was given Na-CMC suspension, group III as positive control was given simvastatin group IV dose 150 mg / kg BW, group V dose 250 mg / kg BW and group VI dose 350 mg / kg BW. The results showed that the ethanol extract of guava leaves influenced reducing blood glucose levels of male white rats with an effective dose of 250mg / kg BW with an average reduction of 119 mg/dL and effective reducing the cholesterol levels at a dose of 150 mg /kg BW with an average reduction of 28,33 mg/dL.

Optimization of Blended Guava and Fluted Pumpkin Leaves extract as corrosion inhibitor of mild steel in 0.5 m hydrochloric acid

&#x0D; &#x0D; &#x0D; There are many plant extracts that have been studied for possible use as corrosion inhibitors in the oil and gas industries. Hence, this work is focused on optimization of blended Guava and Fluted Pumpkin Leaves extract as corrosion inhibitor of mild steel in HCI using Weight loss method. Response Surface Methodology of Design Expert trial version 12 StatEase was used to design and analyze the result of the 35-run experiments. Three factor-three level was adopted in the design. Time, Temperature and Inhibitor Quantity were the independent variables, while the acid concentration of 0.5M was constant throughout the experiments and Inhibition Efficiencies were measured using IE formula. Intervals of 1 hr, 0.2g, and 10°C were chosen for the immersion time, inhibitor quantity and temperature, respectively. Phytochemical analysis carried out on the Guava and Fluted Pumpkin Leaves shows that each leaf extract contains phytochemicals which are responsible for inhibiting corrosion. Weight loss result shows that the Blended extract reduces the corrosion rate of mild steel in 0.5M HCl. Additionally, 4 experiments for Blended Extract, Fluted Pumpkin, Guava Extract and Industrial Inhibitor were carried out at optimal conditions as predicted by the software at time 4.036 hr, temperature 49.5°C, inhibitor quantity 0.487g and ratio of 59.21GE:FP40.79 Inhibition Efficiencies of the Blended, Fluted Pumpkin, Guava Extracts and Industrial Inhibitor were 93.70%, 78.14%, 63.7% and 95.18%, respectively. The results show that the blended and Industrial Inhibitor compared well with the software predicted IE of 96.085%. Therefore, the blended extract could serve as good substitute to the industrial inhibitor.&#x0D; &#x0D; &#x0D;

Comparison of Time-kill Assay to Evaluate the Antimicrobial Efficacy of Garlic (Allium sativum) and Guava (Psidium guajava) Extracts on Periodontal Pathogens.

Background:The role of Gram-negative anaerobic periodontal pathogens in periodontal diseases has led to the loss of tooth-supporting structures. These diseases can be prevented by the inhibition of bacterial biofilm on the tooth surfaces. Many treatment modalities have been tried to prevent periodontal diseases. With the rise in resistance to synthetic antimicrobials, there is a requirement to develop natural antimicrobials for the control of periodontitis.Aim:The aim of the study was to evaluate and compare the efficacy of garlic (Allium sativum) and guava (Psidium guajava) extracts on Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans using time-kill assay.Materials and Methods:Aqueous garlic extract (AGaE), ethanolic garlic extract (EGaE), aqueous guava extract (AGuE), and ethanolic guava extract (EGuE) were prepared. Time-kill assays were performed on P. gingivalis and A. actinomycetemcomitans. The aqueous and ethanolic extracts of guava and garlic were compared to assess the maximum bactericidal potency.Results:The comparison of time-kill assay of AGaE and AGuE on P. gingivalis showed a statistically significant difference at 2 h (t = 5.29, P < 0.01), 4 h (t = −4.867, P < 0.01), and 6 h (t = −3.647, P < 0.001). The comparison of time-kill assay of EGaE and EGuE on A. actinomycetemcomitans showed a statistically significant difference at 2 h (t = 4.54, P < 0.01) and highly significant difference at 4 h (t = 6.57, P < 0.001).Conclusions:The, judicious use of these phytomedicinal products could be cost-effective and also the adverse effects caused due to the long-term usage of synthetic antimicrobials can be avoided.

Guava extract as an antioxidant additive in diets of Japanese breeder quails to mitigate the effect of egg storage time on newly hatched quality

Guava extract as an antioxidant additive in diets of Japanese breeder quails to mitigate the effect of egg storage time on newly hatched quality

FORMULASI, EVALUASI DAN UJI ANTIBAKTERI SEDIAAN GEL EKSTRAK ETANOL DUAN JAMBU BIJI (Psidium guajava L.) SEBAGAI ANTI JERAWAT

Acne is skin problems that can be overcome by using herbal plants, one of which is guava leaves. Guava leaves (Psidium guajava L.) contains tannins, saponins, polyphenols and flavonoids that can inhibit the growth of acne-causing bacteria. This study aims to formulate guava leaf extract (Psidium guajava L.) and evaluate the results of antibacterial tests on gel preparations as anti-acne. The ethanol extract was extracted and formulated using Na-CMC. Evaluation of the antibacterial activity of guava leaf extracts and preparations using Staphylococcus aureus and Eschericia coli bacteria. The results showed that the ethanol extract of guava leaves (Psidium guajava L.) with a concentration of 15% had antibacterial activity against Staphylococcus aureus with an inhibition area of ​​6.75 cm, and the gel preparation of guava leaf ethanol extract (Psidium guajava L.) resistance 0.2 mm. The evaluation found that the gel preparation of ethanol extract of guava leaves (Psidium guajava L.) can be used as an anti-acne gel.

Performance, nutrient digestibility, and intestinal histomorphometry of broilers fed diet supplemented with guava extract standardized in phenolic compounds

The objective of this study was to evaluate the effects of guava extract standardized in phenolic compounds (SGE) on broiler performance, nutrient digestibility, and intestinal histomorphometry. A total of 300 one-day-old male Cobb-500® broiler chicks were distributed in a completely randomized design with five treatments (basal diet supplemented with 120 mg vitamin E/kg and basal diet supplemented with 0, 600, 800, or 1,000 mg SGE/kg) and six replicates of ten birds each. Performance was evaluated at seven and 21 [...]

The effect of guava (Psidium guajava) leaf extract on the quality of cobia (Rachycentron canadum) fillets during ice storage

The aim of this study is to evaluate the effects of Psidium guajava leaves extract on the quality of cobia (Rachycentron canadum) fillets during in ice storage. A group of cobia fillets was soaked in Psidium guajava extract solutions at concentration of 0.03% (w/v) for 30 minutes while the other group soaked in cold ice water, considered as control treatment. The experimental fillets were packed and stored in ice for 15 days. Sampling were done on the 1st, 5th, 10th and 15th day. Analyzed parameters included temperature, total viable counts (TVC), sensory property, pH, moisture, water holding capacity (WHC), texture, total volatile base nitrogen (TVB–N), peroxide value (PV), Thiobarbituric acid reactive substances (TBARs), and fillet color. The results indicated that cobia fillets treated with guava extract solutions 0.03% showed significantly higher sensory property, lower PV and TBARs compared to the control treatment during ice storage. Based on the sensory property and total viable counts, cobia fillets can be used up to 10 days in both treatments remaining high quality of the cobia fillet.

Bioactive compounds present in different parts of Guava and their significance: A review

Guava (Psidium guajava L.) is a tropical and subtropical fruit crop that is commonly used in food and traditional medicine all over the world. It is a member of the Myrtaceae family. Tannins, flavonoid: quercetin, pentacyclic triterpenoid: guajanoic acid, saponins, carotenoids, lectins, leucocyanidin, uvaol, amritoside, betasitosterol, ellagic acid, oleanolic acid, triterpenes, and ursolic acid are some of the essential phytoconstituents. Guava can be eaten raw or refined into juice, pulp, jam, jelly, nectar, powder, tea, and other items. The guava plant parts are utilized for the improvement of variety industrial and medicinal products. Antiviral, anti-inflammatory, antidiabetic and anti-mutagenic factors are all found in guava. Guava extract has antinociceptive properties and can help with liver inflammation and serum development.

Egg characteristics of Japanese quail fed diets containing guava extract (Psidium guajava L.)

The main purpose of this study was to evaluate the characteristics of eggs produced by Japanese quails fed with guava extract. A total of 400 eggs collected from the quails fed with experimental diets, were distributed in a completely randomized design in factorial arrangement 4 x 4 with main effects including four dietary levels of guava extract (0, 3, 6 and 9 g kg-1) and four storage periods (0, 9, 18 and 27 days) totalizing 16 treatments with five replicates of five eggs. The eggs were produced by Japanese quails, fed with experimental diets. The diets were isonutritive formulated by corn-soybean basis. The eggs were stored for different periods, at the same conditions, to constitute the treatments. The parameters evaluated were egg weight loss (g and %), yolk color, Haugh unit, specific gravity, eggshell thickness and pH of the yolk and albumen. Data were submitted to ANOVA, and means were compared using Scott-Knott test, using α = 0.05. The specific gravity of fresh eggs was better with the use of 6 g kg-1 of guava extract in the diet. The use of 3 g kg-1 of guava extract resulted in smaller eggshell thickness of fresh eggs. The Haugh unit was affected just by the storage periods. The dietary guava extract (3, 6 or 9 g kg-1) increased the pH of albumen in eggs stored for 27 days. The use of 6 g kg-1 of guava extract in quails diet increase the specific gravity in fresh eggs and increase the yolk color when the eggs are stored.

Chemical Characterization and Hepatoprotective Effects of a Standardized Triterpenoid-Enriched Guava Leaf Extract

Nutraceutical/pharmaceutical agents capable of maintaining redox and inflammation homeostasis are considered as candidates for the prevention and/or treatment of liver diseases. Psidium guajava (commonly known as guava) leaf is a commercially available functional food that has been reported to possess hepatoprotective property. However, the hepatoprotective constituents in guava leaf are not known. In the current study, a standardized triterpenoid-enriched extract of guava leaves (TGL) was developed. A new ursolic acid derivative, namely 2α,3β,6β,23,30-pentahydroxyurs-11,13(18)-dien-28,20β-olide (1), and 23 known triterpenoids were isolated and identified from TGL. The hepatoprotective effects of TGL were evaluated through a model using acetaminophen (APAP)-exposed C57BL/6 male mice. Pretreatment of TGL (75 and 150 mg/kg) restored the mice hepatic architecture, improved the serum ALT and AST levels, and reduced the hepatic ROS and MDA contents. Further molecular mechanistic study revealed that TGL modulated Nrf2 and MAPK signaling pathways to alleviate APAP-induced oxidative and inflammatory stress in liver. In addition, the new compound 1 from TGL showed protective effects against APAP-induced cytotoxicity via activation of the Nrf2 pathway in HepG2 cells. Overall, this is the first report on the hepatoprotective effects of a standardized triterpenoid-enriched extract of guava leaves, which supports its potential nutraceutical application in liver disease management.

UJI TOKSISITAS EKSTRAK ETANOL DAUN JAMBU BIJI AUSTRALIA (Psidium guajava L) DENGAN METODE BSLT (Brine Shrimp Lethality Test)

ABSTRACTGuava is one of the plants that can traditionally be used for the treatment of diseases. Many kinds of guava, one of which is the Australian guava has the characteristics of roots, stems, leaves, dark red fruit. This study aims to determine the toxicity of the ethanol extract of Australian guava leaves (Psidium guajava L) using the BSLT (Brine Shrimp Lethality Test) method and determine the chemical content of Australian guava leaves (Psidium guajava L). The extract was made by the ultrasonic method using 96% ethanol solvent. Toxicity tests were carried out using 48-hour-old Artemia salina Leach shrimp larvae. The toxic effect of the extract was identified by the percentage of shrimp larvae mortality using probit analysis (LC50). From the research results, phytochemical content includes tannins, flavonoids, alkaloids, terpenoids and saponins, and flavonoid compounds have the highest content compared to the others. Research shows that the ethanol extract of Australian guava leaves is of a moderate category (LC50 441,977 ppm).Keywords :Australia guava leaves, BSLT, Artemia salina L, Ultrasonic

PENGARUH EKSTRAK JAMBU BIJI MERAH (Psidium guajava Linn) TERHADAP KUALITAS DAGING SAPI DALAM PROSES CURING

&lt;p&gt;&lt;em&gt;Curing is a method that can be used to maintain the quality of beef. The curing method uses nitrate and nitrite compounds, which have useful roles in the curing process. However, those compounds have a side effect on the body if consumed continuously, which causes cancer disease (carcinogenic). This harmful carcinogenic property can be controlled by the addition of ascorbic acid. Ascorbic acid is commonly found in several fruits, one of which is red guava (Psidium guajava L)&lt;/em&gt;&lt;em&gt;. &lt;/em&gt;&lt;em&gt;Therefore, this study aims to find out the most optimal addition of red guava extract in maintaining the beef qualities with curing treatment on the parameters of water content, pH, whc, texture, color, ascorbic acid, and nitrite residue. This study was conducted using a Completely Randomized Design with the various concentration of red guava extract (0%-5%) and observed at soaking times of 0, 2, 4, and 6 days. The analysis was performed by twice of sample repetition and analysis repetition. The results of the analysis were statistically analyzed by One Way ANOVA continued with the DMRT test if the results were significantly different (α = 0.05). Based on the results of the study, the higher of concentration in red guava extract, the pH, water content, whc, b* color, a* color, nitrite residue were smaller while the hardness, springiness, cohesiveness, L* color, a* color, the ascorbic acid content in curing were higher. The &lt;/em&gt;&lt;em&gt;chosen treatment&lt;/em&gt;&lt;em&gt; &lt;/em&gt;&lt;em&gt;due to&lt;/em&gt;&lt;em&gt; the test parameter in this study was the addition of 4% concentration in red guava extract in the curing process with 2 days soaking times.&lt;/em&gt;&lt;/p&gt;

The Effect Of Microwave Irradiation on Synthesis of Gold Nanoparticles Using Ethanol Extract of White Bol Guava Leaves

The aim of this research was to determine the effect of microwave radiation on the synthesize of gold nanoparticles using ethanol extract of white bol guava leaves. 50 mL of 25 ppm HAuCl4 solution was synthesized using ethanol extract of white bol guava leaves to convert Au3+ to Au0 with 0.75 mL and synthesized using microwave and without microwave. Characterization of gold nanoparticles (Au-NPs) using Scanning Electron Microscope (SEM) and X-ray Diffraction (XRD) instruments. Based on the results, the microwave irradiation has a significant effect on the formation of Au-NPs using ethanol extracts of white bol guava leaves, based on the analysis using XRD, the size of Au-NPs was 17.13 nm with microwave irradiation and 17.57 nm without without microwave irrdaiation. Based on the results of SEM-EDS obtained Au-NPs by the microwave irradiation of 54.92%, while without a microwave irradiaton of 42.17 %.