Garlic Research Articles

Upregulation of caspase-3, oxidative stress, neurobehavioural and histological alterations in mercury chloride-exposed rats: role of aqueous Allium sativum bulb extract.

Mercury is a highly toxic metal that causes a variety of neurological disorders through oxidative stress. Allium sativum, a cooking spice in diverse cultures around the world, has a long history of medicinal use due to its rich antioxidant constituents. This study was designed to evaluate the protective activity of aqueous Allium sativum bulb extract (ASBE) on mercuric chloride (HgCl2)-induced neurotoxicity. Forty Wistar rats were randomly divided into five groups namely I (control), II (HgCl2; 4mg/kg), III (250mg/kg of ASBE and 4mg/kg of HgCl2), IV (500mg/kg of ASBE and 4mg/kg of HgCl2) and V (500mg/kg of Vitamin E and 4mg/kg of HgCl2). At the end of the administration, neurobehavioural, antioxidant enzymes, lipid peroxidation and apoptotic activities as well as the histology of the cerebrum, cerebellum and hippocampus were assessed. Body and brain weights, locomotion, exploration, cognition, memory and antioxidant enzymes were significantly impaired (p < 0.05) in HgCl2-exposed rats following comparison to control. Lipid peroxidation, mercury concentration and caspase-3 activity were significantly upregulated (p < 0.05) in HgCl2-exposed rats following comparison to control. In addition, significant alterations to the histology of the cerebrum, cerebellum and hippocampus were observed in the HgCl2-exposed rats. Conversely, the adverse effects induced by HgCl2 were significantly attenuated (p < 0.05) following ASBE and Vitamin E pretreatment. Taken together, these results suggest that ABSE exerts its neuroprotective activity through its potent antioxidant and anti-apoptotic properties.

Assessing Syzygium aromaticum: From Chemical Profiling to Antioxidant, Antistaphylococcal, and Biocompatibility Attributes.

Staphylococcal infections challenge current treatments due to adverse effects and drug resistance, prompting the exploration of plant-derived compounds for their promising properties. This study aimed to analyze Syzygium aromaticum essential oil, aqueousand ethyl acetate extracts for antioxidant and antistaphylococcal effects against sensitive and resistant clinical isolates. Erythrocytes were used as model cells for biocompatibility. The SA-EO was extracted via hydrodistillation and compounds were identified using GC-MS. The antioxidant activity was assessed using various tests, while antimicrobial activity was evaluated through disk diffusion and microdilution assays. Results showed Eugenol as the major compound in SA-EO. The AE exhibited high phenolic and flavonoid content. SA-EO demonstrated antioxidant activity with IC50 values of 84.63 ± 1.94 µg/mL (TAC) and 52.88 ± 1.44 µg/mL (FRAP). The AE-SA showed scavenging effects with EC50 values of 266.52 ± 50.62 µg/mL (DPPH) and 140.67 ± 44.36 μg/mL (NO). The antistaphylococcal activity revealed significant sensitivity of SA-EO with MIC and MBC values ranging from 0.316 to 1.266 mg/mL. SA-EO showed low hemolytic activity even at high concentrations. Overall, these findings highlight the potential of Syzygium aromaticum in combating multidrug-resistant bacteria and underscore its role as a source of bioactive compounds with antioxidant properties.

The Analysis of Determinants of Production and Optimization of Garlic Farming in Sembalun District East Lombok Regency

Each farming aims to maximize farming income but is constrained by the quantity of inputs. The limited quantity of input is faced by farmers who cultivate garlic plants. One solution is to utilize production inputs available at the location where farmers live, such as organic fertilizers, to reduce the use of inorganic fertilizers. The purpose of the study is to analyze the influence of the use of production inputs on production quantity and to determine the level of efficiency of the use of each production input. The purpose of the research was achieved through the collection of primary data from the results of the garlic farming survey in Sembalon District, East Lombok Regency. As a respondent, 71 farmers cultivate garlic. The research location is in Sembalun Bumbung village and Sembalun Lawang village. The number of sampling units in each village was selected using a proportional random sampling technique of 44 farmers in Sembalun Bumbung village and 27 farmers in Sembalon Lawang village. Production quantity and input quantity data were analyzed by multiple regression followed by an analysis of the ratio of marginal production value to the price per input unit to determine the status of efficiency in the use of production inputs. The results of the study showed that the use of organic fertilizer inputs, ZA fertilizers, and phonska fertilizers had a real effect on the production quantity. The use of organic fertilizers is not optimal, while the use of ZA fertilizers and phonska fertilizers needs to be reduced to increase efficiency.

The Influence of Gibberellins and Smoke Water as a Stimulant for Germination and Vegetative Growth of Syzygium aromaticum (L.) Merr. &amp; L. M. Perry

Clove or cengkeh (Syzygium aromaticum) is one of Indonesia’s commodities with high domestic and international potential, considering that this plant is used as raw material for the cigarette industry. Therefore, it is necessary to optimize the production of Indonesian cloves, one of which is by using growth stimulators such as plant growth regulators (PGR). This study uses gibberellic acid (GA3) and smoke water as exogenous growth triggers. The treatment given was soaking S. aromaticum seeds in gibberellic acid (GA3) and liquid smoke for 24 h. The GA3 concentrations used were 100 ppm, 75 ppm, 50 ppm, and 25 ppm. Smoke water was obtained from the pyrolysis of coconut shells, and the concentrations used were 0.5%, 1%, 2%, and 3%. Observations were conducted for 11 weeks and divided into two phases, namely the germination phase and the vegetative growth phase. Parameters measured included germination percentage, radicle, and plumula length in the first phase, root length, plant height, and number and area of leaves in the second phase. The best results were achieved with the soaking treatment using 0.5% smoke water, which showed a significant increase in all observed growth parameters. This is due to the content of karrikin in smoke water, which acts like a growth hormone and triggers the performance of other growth hormones. In addition, karrikin plays an active role in the germination process by changing the morphology of the seeds.

Optimizing encapsulation of garlic and cinnamon essential oils in silver nanoparticles for enhanced antifungal activity against Botrytis cinerea pathogenic disease

The application of nanotechnology offers an environmentally sustainable approach to managing plant diseases and improving the protection of agricultural products and food. The antimicrobial potential of essential oils (EOs) derived from Allium sativum (garlic) and Cinnamomum verum (cinnamon) can be significantly enhanced through the application of nanotechnology. In this study, we encapsulated the EOs of A. sativum and C. verum in silver nanoparticles (AgNPs), which resulted in increased fungicidal activity against Botrytis cinerea, the pathogen responsible for anthracnose in many horticultural crops. The encapsulated EOs demonstrated a synergistic effect, achieving over 80% inhibition of B. cinerea mycelium growth and suppressing conidia germination by 75%. Furthermore, substantial morphological changes in the fungal hyphae were observed upon application of the nano-encapsulated EOs. The observed morphological changes included disrupted cell walls and deformed hyphal structures, which indicated severe damage to the fungal cells. The AgNPs function as carriers, enhancing the stability and dispersion of EOs and facilitating a more uniform and sustained release of the active compounds. The results of this study indicate that nano-encapsulated EOs have the potential to be effective antifungal agents, offering a promising approach for pathogen control in agriculture. The integration of nanotechnology with traditional antimicrobial agents enables the development of advanced formulations that provide enhanced protection against a wide range of plant pathogens. This approach not only enhances the efficacy of existing treatments but also reduces the necessity for synthetic chemicals, thereby aligning with sustainable agricultural practices.

Investigating zeolite's role in retaining mineral nitrogen fertilizers and the effects of titanium and selenium on garlic plants under water deficit conditions

Investigating zeolite's role in retaining mineral nitrogen fertilizers and the effects of titanium and selenium on garlic plants under water deficit conditions

Biostimulants for enhancing productivity, bioactive components, and the essential oils of garlic with the potential antifungal activity

To feed the world’s growing population, the agriculture sector has recently had to strike a balance between reducing its detrimental effects on ecosystems and human health and boosting resource efficiency and production. In reality, pesticides and fertilizers are vital to agriculture and are useful instruments that farmers can employ to increase yield and guarantee steady productivity throughout the seasons under both favorable and unfavorable conditions. Therefore, in the present study, fertilizing with potassium citrate as a foliar spray and humic acid (HA) as a soil application allowed for the evaluation of vegetative growth parameters (plant height, number of leaves/plant), total phenolic content, total carbohydrate, antioxidant activity, the essential oil (EO) composition, and bulb yield of garlic (Allium sativum L.). These were carried out in two field experiments throughout the 2020–2021 and 2021–2022 growth seasons. A gas chromatography-mass spectroscopy (GC-MS) apparatus was performed to determine the chemical composition of the isolated EOs. The antifungal activity of the EOs was assessed against two fungi, Fusarium proliferatum and Macrophomina phaseolina, that cause geranium plants to wilt and decay. The findings indicated that applying HA at a rate of 2 g/L with potassium citrate at a rate of 5 or 10 mL/L produced garlic bulbs with the highest levels of productivity and diameter. The diverse treatments between HA with potassium citrate resulted in significant variations in the bioactive components, such as total phenol content, antioxidant activity, total carbohydrate, and sulfur content. The analysis of the EOs revealed the presence of dimethyl trisulfide, diallyl disulfide, methyl 2-propenyl trisulfide, allitridin, and methyl allyl disulfide and allyl tetrasulfide as main compounds. By gradually increasing the concentration of the garlic EO to 4000 µg/mL compared to the control, the inhibition percentage of fungal growth of F. proliferatum and M. phaseolina was increased. In conclusion, a high concentration of HA with potassium citrate (5 or 10 mL/L), may be suitable and highly appreciated as a fertilizer application to enhance the productivity and EOs content of garlic plants.

The Impact of Allicin Contents on Microbiological Counts in Stored Garlic Cloves and Dried Garlic Sheets.

The Impact of Allicin Contents on Microbiological Counts in Stored Garlic Cloves and Dried Garlic Sheets.

In vitro Evaluation of Antifungal Activities of Zingiber officinale (Ginger) and Allium sativum (Garlic) Extracts on Fungal Isolates from Tinea Capitis

In vitro Evaluation of Antifungal Activities of Zingiber officinale (Ginger) and Allium sativum (Garlic) Extracts on Fungal Isolates from Tinea Capitis

Phytogenic synthesis and antimicrobial activity of ZnO nano bow ties (ZnO NBTs): An experimental and computational study

Phytogenic synthesis is a sustainable and eco-friendly approach for producing nanoscale particles, using biological entities such as plants and their byproducts. In this study, Allium sativum extract was selected as a capping and reducing agent due to the presence of phytochemicals such as allicin, diallyl disulfide (DADS), vinyl dithiins, ajoene (E- and Z-ajoene), diallyl trisulfide (DATS), and thiol (sulfhydryl) groups. The resulting ZnO Nano Bow Ties (ZnO NBTs) were characterized using FE-SEM, XRD, EDX, DLS, zeta potential, FTIR, and UV-Vis spectroscopy to evaluate the size, morphology, and crystallinity. The obtained XRD, SEM, and DLS results suggested an average longitudinal length of ∼372 nm with a maximum lateral width of ∼64 nm and a Bow Tie shape. Gas Chromatography-Mass Spectroscopy (GC-MS) analysis was employed to elucidate the prominent phytochemical constituents of the Allium sativum extract. Preliminary antibacterial assays reveal significant inhibition zones and growth inhibition effects against gram-negative bacteria of both Klebsiella pneumoniae and Escherichia coli, suggesting the promising antimicrobial potential of these ZnO NBTs. Monte Carlo simulations revealed that the cone-shaped ZnO NBTs bind strongly to the active sites of the target proteins with binding affinities of −36.20 and −32.14 kcal/mol for Klebsiella pneumoniae and Escherichia coli respectively, which correlates with their activities. The ZnO NBTs complexes formed stronger hydrophobic interactions and hydrogen bonds with amino acid residues of Escherichia coli than with Klebsiella pneumoniae. This integrated experimental and computational study underscores the potential of the use of ZnO NBTs as a sustainable and effective strategy to combat bacterial pathogens. The findings of this study indicate that efficient morphology (shape) is a major contributor to the protein binding affinities of ZnO NBTs, with promising implications for the design of antibacterial drugs in nanomedicine.

Elucidating the genetic basis of bulb-related traits in garlic (Allium sativum) through genome-wide association study

The genetic architecture of garlic bulb related traits were still not well elucidated due to its big and complex genome. In this study, genotyping-by-sequencing (GBS) in 163 garlic accessions mainly from China were conducted. All the 163 garlic accessions were divided into three subpopulations, and largely consistent with geographic origins. Genome-wide association study (GWAS) was conducted for 5 garlic bulb related traits across four environments. Totally, 26 significantly loci were identified in two or more environments and located within or near 431 genes, and explain 14.0–31.7 % of the phenotypic variances. Among these, qBW5.1 was nearly with the qBH5.1. Four loci were reported previously, whereas the remaining 22 are likely to be new. Gene ontology enrichment analysis showed that the candidate genes were significantly enriched in metabolic process, biosynthetic process and catalytic activity. Nine candidate genes encode the zinc finger domain-containing protein, serine/threonine-protein kinase, peroxygenase, auxin-induced protein, ethylene-responsive transcription and E3-Ubiquitin protein ligases were identified and validated. Additionally, a meaningful achievement is one kompetitive allele-specific PCR marker, Kasp_chr7_BW for bulb weight were successfully developed and validated in a diverse panel. These results uncover the genetic mechanism of garlic bulb related traits and provide accessions and KASP markers for further garlic molecular breeding.

Antiferroptotic properties of allicin and related organosulfur compounds—diallyl disulfide and diallyl trisulfide—from Garlic

Allicin (diallyl thiosulfinate) is an abundant bioactive compound in garlic (Allium sativum L.) with broad-spectrum antiinflammatory, antifungal, antioxidant, and antitumor properties. The bioactive compounds of garlic including allicin may also help reduce the incidence of various diseases, although the individual contributions and precise mechanisms are still largely unknown. In this study, we reveal that allicin and the closely related compounds diallyl disulfide and diallyl trisulfide (all containing more than one disulfide bond) protect mouse hippocampal HT22 cells against chemically induced ferroptosis, a newly defined form of cell death characterized by iron-dependent lipid peroxidation. In contrast, these organosulfur compounds did not prevent chemically induced apoptosis. The antiferroptotic efficacies of these compounds were strongly associated with prevention of lipid peroxidation and reactive oxygen species production but independent of glutathione upregulation, ferrous iron chelation, and modulation of the nuclear factor erythroid 2 (NF-E2)-related factor-2-antioxidant element response pathway. Additional studies are warranted on the therapeutic potential of allicin and related compounds in garlic for neurodegenerative diseases associated with ferroptosis.

Physicochemical properties, GC–MS profiling, and antibacterial potential of Allium sativum essential oil: In vitro and in silico approaches

Antibiotic resistance remains a critical challenge for public health. In this study, we investigate the antibacterial potential of Moroccan Allium sativum L. essential oil (EO) through both in vitro and in silico approaches. The EO was extracted using hydrodistillation, and its physicochemical properties were analyzed alongside its chemical composition via gas chromatography-mass spectrometry (GC/MS). We assessed antibacterial efficacy through disk diffusion assays targeting Escherichia coli (E. coli), methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa (P. aeruginosa), and determined the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. Finally, an in silico molecular docking analysis and ADMET (absorption, distribution, metabolism, excretion, and toxicity) prediction were performed. Our analysis identified 13 sulfur-based compounds within the EO, with diallyl disulfide (42.85 %), methylallyl disulfide (18.84 %), methylallyl trisulfide (15.38 %), and diallyl trisulfide (13.11 %) as major constituents. The EO demonstrated robust activity against MRSA, with an inhibition zone of 22 mm and an MIC of 0.75 mg/mL, whereas E. coli exhibited lower sensitivity, with an MIC of 1.51 mg/mL. Molecular docking analysis suggested that the primary sulfur compounds may effectively inhibit bacterial resistance-related enzymes. These findings highlight the potential of Allium sativum L. EO as a natural antibacterial agent, attributed largely to its sulfur compound content. This activity is likely linked with the EO's interaction with the complex structures of bacterial cell membranes, offering promise in combating resistant bacterial strains.

Effect of Pre-Sowing Treatments on Cloves Germination and Bulb Yield and Quality of Garlic Plants

Effect of Pre-Sowing Treatments on Cloves Germination and Bulb Yield and Quality of Garlic Plants

Response of Garlic Plants to Foliar Spray by Biostimulating Treatments

Response of Garlic Plants to Foliar Spray by Biostimulating Treatments

Development and characterization of material resulting from the sulfation of chitosan incorporated with essential oils for application in the control of dermatophyte fungus

Chitosan (CS) is a natural polymer widely used in the development of various materials. Chemical modifications to its structure guarantee an improvement in its physicochemical and bioactive properties. Therefore, in this study, sulfation of chitosan (CS-S) and incorporation of essential oils (EOs) of Syzygium aromaticum and Cinnamomum ssp. were carried out, with the aim of obtaining a material to be applied in the control of dermatophytosis caused by the fungus Microsporum canis. The material obtained was characterized using FTIR, 13C SSNMR, TGA-MS and SEM techniques. The presence of sulfate groups causes the material to have greater solubility in aqueous media (4.18 ± 1.1 g L−1) compared to Chitosan (0.70 ± 0.6 g L−1). Incorporation efficiency (IE) demonstrated that CS-S has the ability to incorporate EOs with values close to 21 % (W/W). Dynamic light scattering (DLS) studies showed that CS-S particles had a manometric size (CS-S-EOC 960.6 ± 161.2 nm and CS-S-EOS 414.1 ± 118.0 nm), when in aqueous medium, after EO incorporation. The antifungal potential was evaluated for CS-S-EOC and CS-S-EOS, with values of 59.67 % and 64.93 %, respectively, demonstrating inhibition potential on the mycelial growth of the fungus Microsporum canis.

Allium sativum nanovesicles exhibit anti-inflammatory and antifibrotic activity in a bleomycin-induced lung fibrosis model.

Idiopathic pulmonary fibrosis (IPF) is a chronic and highly fatal disease characterized by excessive accumulation of extracellular matrix (ECM), foci of myofibroblasts, and a usual pattern of interstitial pneumonia. As suggested by international guidelines, the treatment for this disease involves supportive therapies, as there is currently no effective treatment. Plant-derived nanovesicles have emerged as a new treatment for various diseases and have been tested in cellular and murine models. This research aimed to test the use of Allium sativum nanovesicles (AS-NV) in a murine model of IPF induced by bleomycin. AS-NV reduced the amount of collagen and restored lung architecture in the mouse model. AS-NV was tested on human lung fibroblasts, which do not affect the viability of healthy cells. AS-NV treatment decreases the mRNA levels of genes related to fibrosis, inflammation, and ECM deposition (Mmp2,Timp-2,Vegf,Pcna,Col1a1,Tgf-β,α-Sma,IL-1β,and Hif1a) in bleomycin-induced idiopathic pulmonary fibrosis. This research highlights the anti-inflammatory and antifibrotic activity of AS-NV, which contributes to plant nanovesicle mechanisms in IPF; however, more AS-NV studies are needed to identify alternative treatments for idiopathic pulmonary fibrosis.

Exploring the Antibacterial Properties and Chemical Profiles of Essential Oils from Syzygium aromaticum and Nigella sativa Against MDR Bacteria

For centuries, plants have been studied for their healing properties. Recently, the rise of antibiotic resistance in bacteria has prompted scientists to explore herbal remedies. Syzygium aromaticum, from the Myrtaceae family, has been utilized as a histological clearing agent. In contrast, Nigella sativa (black cumin), a member of the Ranunculaceae family, has long been utilized in traditional medicine dating back to ancient times. The current study investigates the chemical composition and antibacterial activity of essential oils from Syzygium aromaticum (clove) and Nigella sativa (black cumin) against multidrug-resistant (MDR) bacteria. Using the agar well diffusion method, Syzygium aromaticum exhibited significant antibacterial activity, particularly against Salmonella typhi (30.66 mm inhibition zone), while Nigella sativa was effective only against Staphylococcus aureus (19 mm). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values for Syzygium aromaticum ranged from 3.53 to 7.06 mg/mL, indicating stronger antibacterial properties than Nigella sativa (MIC: 12.7 mg/mL, MBC: 22.5 mg/mL). GC-MS analysis revealed that Syzygium aromaticum contained bioactive compounds like Diglycolic acid (76.59%) and Eugenol (2.06%), while Nigella sativa was dominated by Heptasiloxane (80.83%) and Thymoquinone (2.66%). These findings suggest that Syzygium aromaticum essential oil could serve as a potent natural antimicrobial agent against MDR pathogens.

Combined Transcriptomic and Metabolomic Analyses Reveal the Mechanisms by Which the Interaction Between Sulfur and Nitrogen Affects Garlic Yield and Quality

Nitrogen and sulfur are essential macronutrients in plant growth and development, and their interaction profoundly influences gene expression, metabolic activities, and adaptability in plants, directly affecting plant growth and yield. Garlic (Allium sativum L.) is a crop of significant economic and medicinal value. However, despite the critical role of the nitrogen–sulfur interaction in garlic’s adaptability, yield, and quality, the specific mechanisms underlying these effects remain unclear. In this study, transcriptomic and metabolomic analyses were employed to investigate the effects of combined sulfur and nitrogen application on garlic bulb tissues. The results show that the combined application of sulfur and nitrogen significantly increased the diameter and weight of garlic bulbs by 14.96% and 35.47%, respectively. The content of alliin increased by 28.48%, while the levels of abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA), and gibberellin (GA) increased by 15.82%, 12.94%, 32.34%, and 48.13%, respectively. Additionally, the activities of alliinase, superoxide dismutase (SOD), and catalase (CAT) were enhanced by 7.93%, 4.48%, and 19.74%, respectively. Moreover, the application of sulfur and nitrogen significantly reduced the malondialdehyde (MDA) content and peroxidase (POD) activity in garlic bulbs by 29.66% and 9.42%, respectively, thereby improving garlic’s adaptability and growth potential. Transcriptomic analysis revealed differentially expressed genes in several key pathways, including plant hormone signal transduction, RNA degradation, glutathione metabolism, amino acid biosynthesis, and glycerophospholipid metabolism. Metabolomic analysis identified 80 differentially abundant metabolites primarily consisting of amino acids, indole carboxylic acids, and fatty acids. The integrated transcriptomic and metabolomic analyses highlighted the pivotal roles of glutathione metabolism, glycerophospholipid metabolism, and amino acid biosynthesis pathways in the synergistic effects of sulfur and nitrogen. This study not only provides critical scientific evidence for understanding the mechanisms underlying the nitrogen–sulfur interaction’s impact on the yield and quality of garlic but also offers a scientific basis for optimizing nutrient management strategies to enhance garlic yield and quality.

Safety assessment and exploration of the mechanism of toxic effects of diallyl trisulfide: Based on acute and subacute toxicity and proteomics experiments

Ethnopharmacological relevanceGarlic (Allium sativum L.) is a widely consumed spice and condiment around the world, applied both as a food and as a traditional medicine, and is a natural strengthening agent for the body's circulatory and nervous systems. Diallyl trisulfide (DATS) is the major volatile organosulfur phytochemical found in garlic, with antithrombotic, anticoagulant, and antiplatelet activities as well as antioxidant, anti-infective, and other pharmacological effects. However, the safe dose and the underlying mechanisms of its toxic effects remain elusive. Aim of the studyDATS, an important pharmacologically active compound found in garlic, has garnered attention for its ability to fight cancer, antioxidant, anti-infective, and cardioprotective. The aim of this study was to evaluate the safety of DATS and to elucidate the potential mechanisms of its toxicity. Materials and methodsIn this study, ICR mice were selected for acute and subacute toxicity experiments according to OECD guidelines. The toxicity profile of DATS was analyzed by computer prediction software. Also, key differential proteins in spleen and serum were analyzed by proteomics. The binding stability of DAST to differential proteins was analyzed by molecular docking. Additionally, the regulatory relationship between DATS and differential proteins was verified by Western blot and ELISA experiments. ResultsThe results showed that the LD50 value of DATS in acute toxicity was 188.67 mg/kg. In subacute toxicity, water consumption and food intake were reduced in both male and female mice. In addition, the spleen and small intestinal organ coefficients were significantly elevated in male mice at the high dose of DATS; the blood biochemical indices ALB and TP were also significantly elevated. HE staining results showed significant damage to the spleen, liver, small intestine, and kidney of mice at high doses of DATS. Spleen and serum proteomics analyses showed that DATS significantly inhibited ZBP1 expression and upregulated TEC. ADMETlab 2.0 software predictions identified DATS as having potential genotoxicity, dermal sensitization, carcinogenicity, and respiratory and ocular toxicity. Docking results showed that the binding energies between DATS and TEC protein (PDB: 6F3F) and ZBP1 protein (PDB: 4KA4) were −3.7 kcal/mol, −2.4 kcal/mol, respectively. Western blot results showed that DATS-H significantly inhibited the expression of ZBP1 (only in male mice) and Bcl-2 proteins. ELISA results showed that DATS-H significantly increased the level of TEC protein both in male and female mice. ConclusionsLong-term administration of high-dose DATS may carry some risk of toxicity. Based on the amount of DATS in garlic, it is recommended that adults should not take more than 359 mg of DATS and 84.5 g of garlic per day. The mechanism of toxicity may be related to the fact that DATS significantly inhibits ZBP1 expression, upregulates TEC, and promotes apoptosis. This study provides valuable toxicological data for the effective evaluation of the long-term toxicity of DATS and offers an additional experimental basis for developing DATS as a healthy food or drug.