This paper discusses recovery of erythromycin (EMC) from the solvent rich phase formed after sugaring-out extraction (SOE), a novel two phase separation technique. The conditions for SOE of EMC have already been optimized (glucose 15.6% w/w, temperature 4 °C, pH 8.3, and solvent/broth ratio 1:1). The solvent i.e. acetonitrile (ACN) rich phase was further processed to separate EMC using crystallization. Three different modes of crystallization of EMC were studied for a model system: evaporative, antisolvent (water), and combined antisolvent-evaporative. It was found that EMC degraded during evaporative crystallization, substantially lowering the yield. Antisolvent crystallization at the EMC concentration used did not yield any crystals. The combined antisolvent-evaporative crystallization resulted in the formation of desirable plate like crystals with a yield of 95% and was explored further using simulated fermentation broth (SFB) which resulted in a yield of 92.2% with desired crystal morphology. A mathematical model for EMC crystallization was also developed and the crystallization kinetics was estimated by fitting model predictions with the experimental data. Furthermore, antimicrobial activity of the crystallized EMC was found comparable to the commercial sample, showing that the chosen purification technique did not impact the efficacy of the recovered EMC.