With the progress of globalization, the environment for enterprises’ survival and development has become increasingly complex. More and more enterprises realize that their sustainable competitive advantage is closely related to the development of enterprise niche. Based on the ecostate-ecorole theory, an evaluation index system for enterprise niche is developed in this paper. The study selects indicators based on literature research and frequency analysis, adopts factors including market environment, industrial environment, human resources, and technical resources to evaluate ecostate of enterprise niche, and establishes an evaluation model for ecostate; the research uses factors including policy environment, innovation decision-making ability, resource accessibility, and technical management capability to evaluate ecorole of enterprise niche, and sets up an evaluation model for ecorole by catastrophe progression method. The results of the reliability and validity test showed that the evaluation index system is both reliable and effective. The paper provides implications for the evaluation of enterprise niche.