Integration of Chelex-100 thermal lysis and lyophilized LAMP for rapid point-of-care detection of surgical site infections following liver surgery.

Standard laboratory tests for surface disinfectants often fail to reflect real-life clinical conditions, potentially overestimating efficacy. Simulated-use testing that incorporates clinical strains, realistic contamination and user application may provide a more accurate reflection of in-use performance in healthcare settings. The aim of this study was to develop and validate a standardized, reproducible Phase 3 Step 1 simulated-use surface disinfection test that incorporates clinically relevant organisms, hospital-representative surfaces, and realistic application methods. Based on EN 16615:2015, the test method was modified to reflect hospital conditions more closely. Clinically isolated outbreak strains of Staphylococcus aureus, Enterococcus faecium and Acinetobacter baumannii were used. Contamination was applied via a touch-transfer method. Surface materials included hospital-relevant substrates, and disinfectant wipes were applied by trained volunteers to simulate routine cleaning practices. The touch-transfer contamination method was reproducible, and no significant differences were observed in drying or water controls across different surfaces. Wiping speed and contact pressure did not correlate with efficacy. However, microbial recovery varied across test runs and participants. The test method presented here allows for efficacy testing of commercial disinfectants. A Phase 3 Step 1 simulated-use test was established, which incorporates micro-organisms isolated from the application area, surfaces representative of the application area, and where the product is applied by trained participants. This internally validated method better represents clinical disinfection practices compared with current standardized tests and may support improved assessment of surface disinfectant efficacy under conditions approximating real-world hospital use.

Clinical characteristics and mortality risk factors in enterococcal bloodstream infections: a 9-year retrospective cohort study in Japan.

Plasmonic magnetic nanoparticles-enabled universal enrichment, photothermal lysis, and duplex CRISPR detection of bacteria in urine samples.

Synthesis of pyrazine derivative-substituted phthalocyanines: evaluation of their Sono/photochemical and antimicrobial properties supported by DFT studies.

Chemical, physical, and antibacterial characteristics of hydraulic calcium silicate sealer modified with hydroxyapatite, carboxymethyl chitosan, and poly[2-(methacryloyloxy)ethyl]trimethylammonium chloride.

Anti-biofilm and associated anti-virulence activities of sucrose fatty acid esters against human pathogens.

Design and synthesis of benzimidazole-based derivatives with antimicrobial activity: mechanistic insights into ROS-mediated oxidative damage, hemolytic assessment, and molecular docking studies.

Efficacy of common disinfectants used in Malaysia against isolates of vancomycin-resistant enterococci (VRE) on surfaces simulating poultry farm environment.

Characteristics of reproductive tract microbiota in health and disease.

Rifaximin in cirrhosis: when therapeutic drift meets antimicrobial stewardship.

Tackling antibiotic resistance in ESKAPE pathogens through the lens of bacterial small RNAs.

Limited utility of MALDI-TOF MS for identifying optrA-carrying vancomycin-resistant Enterococcus faecium during a hospital outbreak.

Innovative synbiotic functional fermented barley-based milk using novel isolated probiotic Levilactobacillus brevis HBE2 and Enterococcus faecalis HBE4

Bidirectional modulation of microbial communities by tea polyphenols and gallic acid enhances quality in dry fermented sausages.

The pursuit of eco-friendly nanomaterials with both antibacterial and anticancer properties is gaining momentum in biomedical research. This study reports the green synthesis of Silver and Zinc oxide (Ag/ZnO) nanocomposite using Coleus amboinicus leaf extract as a natural reducing and stabilizing agent. Characterization via UV–Vis spectroscopy revealed absorption peaks at approximately 280 nm and 370 nm, indicative of Ag and ZnO nanoparticles, respectively. FTIR analysis identified phytochemical-derived functional groups responsible for particle stabilization, while SEM imaging showed agglomerated nanoparticles with diameters ranging from 100 to 150 nm. XRD analysis confirmed the crystalline nature of the nanocomposites. The biosynthesized Ag/ZnO nanocomposite exhibited potent antibacterial activity against multidrug-resistant strains including Enterococcus faecalis , Pseudomonas aeruginosa , MRSA, and Klebsiella pneumoniae , with significant zones of inhibition observed at a concentration of 80 μg/mL. Furthermore, the nanocomposite displayed promising anticancer activity against A549 lung cancer cells, achieving an IC₅₀ of 60 μg/mL after 24 h, as determined by MTT assay. Morphological features of apoptosis, such as membrane blebbing and nuclear condensation, were observed, while AO/EtBr, DCFDA, and Rhodamine 123 staining confirmed elevated reactive oxygen species (ROS) generation and disruption of mitochondrial membrane potential. These findings suggest a ROS mediated mechanism underlying the dual antibacterial and anticancer activities of the Ag/ZnO nanocomposite. This green synthesis approach provides a sustainable strategy for developing multifunctional nanomaterials, although further in vivo studies are required to confirm efficacy and elucidate the molecular mechanisms involved for future therapeutic applications. • Green synthesis of Ag/ZnO nanocomposite using Camboinicus leaf extract • Potent activity against MDR bacteria at 80 μg/mL concentration • Anticancer efficacy against A549 lung cancer cells • ROS-mediated mechanism confirmed via multiple fluorescence staining assays.

Phage-loaded chitosan-polyvinyl alcohol (Ph-CH/PVA) hydrogel for targeted eradication of drug-resistant Enterococcus faecalis and enhanced wound healing in a murine model

Molecular and functional characterization of Enterococcus faecalis OB15: A hypoallergenic and antimicrobial starter from Tunisian Rigouta cheese

Antimicrobial activities of new and known flavanoid metabolites of Dalea lanata var. terminalis (Fabaceae).

Unique structural features and DNA recognition mechanisms of the PRD-containing virulence regulators MafR and MgaSpn.