Enterobacter Cancerogenus Research Articles

Detección e identificación molecular de Pantoea vagans en flores de Dahlia sp.

Las Dalias (Dahlia spp.) son flores nativas de Mesoamérica y endémicas de México. Su consumo como alimento es una práctica antigua, en la actualidad es escasa la información y reglamentación sanitaria para su comercialización y consumo. Al respecto, el objetivo del presente estudio fue identificar morfológica, bioquímica y molecularmente, bacterias de origen entérico asociadas con flores de Dalia. Los resultados de la caracterización morfológica revelaron la predominancia de bacilos cortos Gram negativos. A partir de la observación de la morfología colonial en medios de cultivo selectivos y diferenciales se identificaron como Escherichia coli y Salmonella spp. Sin embargo, el uso de un método comercial automatizado los clasifica como Enterobacter cancerogenus. Debido a que ambas pruebas arrojaron resultados distintos en cuanto a la identidad del microorganismo aislado, no fueron concluyentes. Por lo tanto, se recurrió a la caracterización molecular para la identificación del aislado bacteriano. La bacteria predominante en flores de Dalia, correspondió a Pantoea vagans (Número de acceso CP002206). Éste es el primer reporte de Pantoea vagans aislada de flores de Dalia.

Characterization and Identification of Cellulose-degrading Bacteria Isolated from a Microbial Fuel Cell Reactor

Electricity can be directly biogenerated by bacteria in a microbial fuel cell (MFC) using many different biodegradable wastes as substrate. When cellulose is used as a substrate, the cellulolytic and electrogenic activities require a microbial consortium for energy generation. In this study, cellulose-degrading bacteria were isolated from an MFC using CMC (carboxymethylcellulose) agar medium and their cellulolytic activity was assessed. Cellulolytic bacteria isolated from the MFC were characterized and identified based on their phenotypic characteristics and analysis of their 16S rRNA genes sequence. Of thirty-two isolates, only ten cellulolytic bacterial strains were successfully isolated from the MFC reactor under aerobic conditions. The bacterial isolates had a cellulolytic index between 3.63 to 8.96 U mL-1. The bacterial strain SAM3a demonstrated high identity (99% via 16S-rRNA sequencing) to Staphylococcus saprophyticus which showed the highest CMCase activity (8.96) 0.34U mL-1). Enterobacter cancerogenus JCT-55 showed the next highest CMCase activity (8.34 ± 0.56 U mL-1); S. epidermidis BAB-2554 showed the lowest CMCase activity (3.63) 0.05 U mL-1). In the MFC, the genus Staphylococcus was found to be the most dominant group of cellulose-degrading bacteria which used rice straw as a carbon source. In this study, Escherichia coli, S. saprophyticus, Enterobacter cancerogenus, S. epidermidis, S. hominis, Bacillus subtilis, L. murinus, S. haemolyticus, S. epidermidis, and S. epidermidis were found to possess cellulolytic activity.

Enzymatic Inactivation of Pathogenic and Nonpathogenic Bacteria in Biofilms in Combination with Chlorine

This study investigated the effects of enzyme application on biofilms of bacterial isolates from a cafeteria kitchen and foodborne pathogens and the susceptibility of Salmonella biofilms to proteinase K combined with chlorine treatment. For four isolates from a cafeteria kitchen ( Acinetobacter, Enterobacter, and Kocuria) and six strains of foodborne pathogens ( Salmonella enterica, Staphylococcus aureus, and Vibrio parahaemolyticus), the inhibitory effect of enzymes on biofilm formation at 25°C for 24 h or the degradative efficacy of enzymes on 24-h mature biofilm at 37°C for 1 h in tryptic soy broth (TSB) was examined in a polystyrene microtiter plate. The effect of enzymes was also evaluated on a subset of these strains in 20 times diluted TSB (1/20 TSB) at 25°C. The working concentrations of five enzymes were 1 U/100 μL for α-amylase, amyloglucosidase, cellulase, and DNase and 1 milli-Anson unit/100 μL for proteinase K. In addition, 24-h mature Salmonella Typhimurium biofilm on a stainless steel coupon was treated with proteinase K for 1 h at 25°C followed by 20 ppm of chlorine for 1 min at 25°C. The results showed that certain enzymes inhibited biofilm formation by the kitchen-originated bacteria; however, the enzymatic effect was diminished on the mature biofilms. Biofilm formation of V. parahaemolyticus was suppressed by all tested enzymes, whereas the mature biofilm was degraded by α-amylase, DNase I, and proteinase K. Proteinase K was effective in controlling Salmonella biofilms, whereas a strain-dependent variation was observed in S. aureus biofilms. In 1/20 TSB, Enterobacter cancerogenus and Kocuria varians were more susceptible to certain enzymes during biofilm formation than those in TSB, whereas the enzymatic effect was much decreased on 24-h mature biofilms, regardless of nutrient conditions. Furthermore, synergistic inactivation of Salmonella Typhimurium in biofilms was observed in the combined treatment of proteinase K followed by chlorine. Live/Dead assays also revealed a decrease in density and loss of membrane integrity in Salmonella Typhimurium biofilms exposed to the combined treatment. Therefore, certain enzymes can control biofilms of isolates residing in a cafeteria kitchen and foodborne pathogens. This study demonstrates the potential of enzymes for the sanitation of food processing environments and of proteinase K combined with chlorine to control Salmonella biofilms on food contact surfaces.

Characterization of Diesel Degrading <i>Enterobacter cancerogenus</i> DA1 from Contaminated Soil

The petroleum industry is an important part of the world economy. However, the massive exposure of petroleum in nature is a major cause of environmental pollution. Therefore, the microbial mediated biodegradation of petroleum residues is an emerging scientific approach used to resolve these problem. Through the screening of diesel contaminated soil we isolated a rapid phenanthrene and a diesel degrading bacterium identified as Enterobacter cancerogenus DA1 strain through 16S rRNA gene sequence analysis. The strain was registered in NCBI with an accession number MG270576. The optimal growth condition of the DA1 strain was determined at pH 8 and 35°C, and the highest degradation rate of the diesel was achieved at this condition. At the optimal condition, growth of the strain on the medium containing 0.05% phenanthrene and 0.1% of diesel-fuel was highest at 45 h and 60 h respectively after the incubation period. Biofilm formation was found significantly higher at 35°C as compared to 30°C and 40°C. Likewise, the lipase activity was found significantly higher at 48 h after the incubation compared to 24 h and 72 h. These results suggest that the Enterobacter cancerogenus DA1 could be an efficient candidate, for application through ecofriendly scientific approach, for the biodegradation of petroleum products like diesel.

Structural Basis for DNA Recognition of a Single-stranded DNA-binding Protein from Enterobacter Phage Enc34

Modern DNA sequencing capabilities have led to the discovery of a large number of new bacteriophage genomes, which are a rich source of novel proteins with an unidentified biological role. The genome of Enterobacter cancerogenus bacteriophage Enc34 contains several proteins of unknown function that are nevertheless conserved among distantly related phages. Here, we report the crystal structure of a conserved Enc34 replication protein ORF6 which contains a domain of unknown function DUF2815. Despite the low (~15%) sequence identity, the Enc34 ORF6 structurally resembles the gene 2.5 protein from bacteriophage T7, and likewise is a single-stranded DNA (ssDNA)-binding protein (SSB) that consists of a variation of the oligosaccharide/oligonucleotide-binding (OB)-fold and an unstructured C-terminal segment. We further report the crystal structure of a C-terminally truncated ORF6 in complex with an ssDNA oligonucleotide that reveals a DNA-binding mode involving two aromatic stacks and multiple electrostatic interactions, with implications for a common ssDNA recognition mechanism for all T7-type SSBs.

The prevalence of antibiotic-resistant bacteria (ARB) in waters of the Lower Ballona Creek Watershed, Los Angeles County, California.

Screening for the prevalence of antibiotic-resistant bacteria (ARB) was done at the Ballona Creek and Wetlands, an urban-impacted wetland system in Los Angeles, California. The goals were (1) to assess the overall prevalence of ARB, and (2) compare differences in ARB abundance and the types of antibiotic resistance (AR) among the following sample types: lagoon water from Del Rey Lagoon, urban runoff from Ballona Creek, and water from the Ballona Wetlands (tidal water flooding in from the adjacent estuary, and ebbing out from the salt marsh). Antibiotic resistance distributions were analyzed using the Kolmogorov-Smirnov test to develop the cumulative frequency of bacteria having resistance of up to eight antibiotics. Distributions from the environmental water samples were compared to unchlorinated secondary effluent from the Hyperion Water Reclamation Plant that was used as comparator samples likely to have an abundance of ARB. As expected, densities of total and ARB were highest in secondary effluent, followed by urban runoff. Samples of water flooding into the wetlands showed similar results to urban runoff; however, a reduction in densities of total and ARB occurred in water ebbing out of the wetlands. During preliminary work to identify ARB species, several bacterial species of relevance to human illness (e.g., Staphylococcus aureus, Enterococcus hirae, Pseudomonas aeruginosa, Aeromonas veronii, Enterobacter cancerogenus, Serratia marcescens, Pseudomonas stutzeri, and Staphylococcus intermedius) were isolated from sampled waters. If wetlands are a sink for ARB, construction and restoration of wetlands can help in the mediation of this human and environmental health concern.

Feasibility study of reduction of nitroaromatic compounds using indigenous azo dye-decolorizers

This first-attempt study assessed and compared eight model indigenous dye-decolorizing bacteria strains (e.g., Aeromonas hydrophila NIU01, Aeromonas salmonicida 741,760 and 829, A. tecta 644, Enterobacter cancerogenus BYm30, Exiguobacterium acetylicum NIU-K2, and Ex. indicum NIU-K4) for reduction of nitroaromatic compounds (NACs) to aminoaromatic compounds in “cell pellet” (CP) cultures. Possibly due to identical reductases originated from azoreductases and nitroreductases, indigenous decolorizing bacteria also owned promising nitro reduction characteristics. UV–vis spectroscopy and HPLC chromatography studies revealed that the strains have significant azo dye-decolorizing capabilities, and also can reduce 4-nitrobenzoic acid (4-NBA) to 4-aminobenzoic acid (4-ABA). Nitro reduction performance of indigenous bacterial strains were revealed via determination of specific reduction rate (SRR) of 4-NBA to 4-ABA. The ranking of SRRs of eight indigenous bacterial strains to reduce 4-NBA revealed that NIU01 had the greatest reduction potential; however, NIU-K4 had the least SRR in LB-removed cultures. The multiple-spectrum reduction properties of these indigenous decolorizer strains may be applicable to the treatment of various organic pollutants (e.g., azo dyes and NACs).

Antifungal properties exhibited by bacteria isolated from agriculturally cultivable soils and their antagonistic nature towards fungal phytopathogen suppression

Soil samples collected from rhizosphere soil, cultivable field soil, Rhizoplane soil of paddy plants and were used to isolate bacteria by culture methods of which thirty two bacterial cultures were identified with diverse colony characteristics on nutrient agar medium and biochemical characterization. Sixteen bacterial isolates were selected for screening fungal antagonism on PDA and were further tested against <italic>Fusarium</italic> and <italic>Rhizoctonia species.</italic> In this study, two <italic>Pseudomonas strains</italic> (RB15, RB30) and <italic>Bacillus cereus</italic> (RB13) were cyanogen producers. Both strains of <italic>Pseudomonas</italic> RB15 and RB30 and <italic>Bacillus cereus</italic> (RB13) were also siderophore producers. Only three isolates showed chitinolytic activity; <italic>Serratia marcesens (RB24), Bacillus cereus (RB13) and Enterobacter cancerogenus</italic> (RB17).

Feasibility Study on Dye Decolorization Using Microbial Cultures Supplemented with Decolorized Metabolites of <i>Hylocereus polyrhizus</i>

This feasibility study used indigenous microbes to implement assessment upon capabilites of dye decolorization with supplementation of decolorized metabolites (DM) of Hylocereus polyrhizus. As indicated, ca. 5% DM of Hylocereus polyrhizus could clearly exhibit reversible redox peak potentials via cyclic voltammetric analysis, revealing its significant capability as electron-shuttling mediators. Compared to DM-supplemented decolorization, the ranking of dye-decolorizing capabilities of bacteria was Shewanella putrefaciens WLP72> Enterobacter cancerogenus BYm30> Aeromonas hydrophila NIU01~ A. hydrophila YTl1> Klebsiella pneumoniae ZMd31. The ranking of biodegradability of azo dyes was RB160 > RBk5 > DY86˜ =RG19 > RR141. Evidently, the presence of DM significantly stimulated bacterial decolorization performance of WLP72, BYm30, ZMd31. However, possibly due to inhibitory characteristics of DM to A. hydrophila, its dye decolorization was delayed. That is, using DM to enhance dye decolorization, biodecolorizers should be acclimated in such envionments for effective expression. The findings also pointed out that supplementation of DM to YTl1, WLP72 at ca. 10~15 % for decolorization of RR141 could effectively increase dye-decolorizing efficiency ca. 2.33~2.88 fold. Such DM augmentation inevitably could autocatalytically stimulate electron transfer capabilities for optimal wastewater decolorization with sustainable development.

Surgical Site Infection Caused by Enterobacter cancerogenus

We report the first case of surgical site infection caused by Enterobacter cancerogenus. The infection occurred in a patient who underwent surgery for a broken ankle. The source of infection was unclear and clinical outcome was good after treatment with amoxicillin/clavulanic acid for 10 days. The review of the literature shows that E. cancerogenus has been rarely implicated in skin and soft tissue infections. These infections occur usually after injuries or trauma related to environmental contamination. In conclusion, E. cancerogenus should be considered as a potential pathogen in patients with surgical site infections. Further cases should be reported in the future to know better the transmission routes of E. cancerogenus in patients with nosocomial infections.

Isolation and Characterization of Salt Tolerant Endophytic and Rhizospheric Plant Growth-Promoting Bacteria (PGPB) Associated with the Halophyte Plant (Sesuvium Verrucosum) Grown in KSA

This study was designed to isolate and characterize endophytic and rhizospheric bacteria associated with the halophyte plant Sesuvium verrucosum, grown under extreme salinity soil in Jeddah, Saudi Arabia. The plant growth promotion activities of isolated bacterial were evaluated in vitro. A total of 19 salt tolerant endophytic and rhizospheric bacterial isolates were obtained and grouped into six according to genetic similarity based on RAPD data. These six isolates were identified by amplification and partial sequences of 16S rDNA as Enterobacter cancerogenus,Vibrio cholerae, Bacillus subtilis, Escherichia coli and two Enterobacter sp. Isolates were then grown until exponential growth phase to evaluate the atmospheric nitrogen fixation, phosphate solubilization, and production of phytohormones such as indole-3-acetic acid, as well as 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. While, All of the six strains were negative for ACC deaminaseactivity, two isolates showed Nitrogen fixation activity, three isolates produce the plant hormone (Indole acetic acid) and two isolates have the activity of solubiliztion of organic phosphate. Among the six isolates, the isolate (R3) from the soil around the roots is able to perform the three previous growth promoting possibilities together and it is ideal for use in promoting the growth of plants under the high salinity conditions. This isolate is candidate to prepare a friendly biofertelizer that can be used for the improvement of the crops performance under salinity conditions.Int J Appl Sci Biotechnol, Vol 3(3): 552-560

Antibiotic Resistance and Molecular Characterization of Enterobacter cancerogenus Isolated from Wild Birds in Taif Province, Saudi Arabia

Antibiotic Resistance and Molecular Characterization of Enterobacter cancerogenus Isolated from Wild Birds in Taif Province, Saudi Arabia

Isolation of Alkaline-tolerant Bacteria from Primary Infected Root Canals

IntroductionAlkaline-tolerant bacteria in primary infected root canals could have enhanced survival capacity against antimicrobials commonly used in root canal treatment. The aims of this study were to isolate and characterize alkaline-tolerant bacteria before endodontic treatment (S1), after chemomechanical root canal preparation (S2), and after calcium hydroxide dressing (S3). MethodsBacteriologic samples were obtained from 43 primary infected root canals. Samples were inoculated into culture media at a pH of 9 and incubated anaerobically. The identities of bacterial isolates were determined by 16S ribosomal RNA sequencing. ResultsAll S1 samples were culture positive, with 70% harboring bacteria tolerating a pH of 9. Gram-positive bacteria Pseudoramibacter alactolyticus and Streptococcus spp were the most frequently isolated strains with a prevalence of 54%. Of 13 culture-positive S2 samples, 8 isolates tolerated a pH of 9, namely Streptococcus sanguinis, Enterococcus faecalis, Enterobacter cancerogenus, Streptococcus oralis, and Fusobacterium nucleatum. Seven of these 8 isolates (88%) were correspondingly isolated at S1. All 3 culture-positive S3 samples tolerated a pH of 9, namely S. sanguinis and E. faecalis, which were also isolated in the corresponding S1 and S2 samples. ConclusionsWe showed that the presence of alkaline-tolerant Streptococcus and Enterococcus spp in primary infected root canals could lead to their persistence during and after root canal treatment and could pose a challenge to current treatment efficacy.

Microbial biofilm development on neonatal enteral feeding tubes.

Neonates in intensive care units often require supporting medical devices and antibiotic treatment. The intensive care treatment combined with their immature immune system, the increased permeability of mucosa, and the undeveloped microflora of the gut may render the neonates highly vulnerable to colonisation and subsequent infections when exposed to opportunistic pathogens. These infections may not only be local gastrointestinal infections, but also systematic following translocation from the gastrointestinal system. This could be particularly alarming considering that common antibiotics may not be effective if the causative strain is multi-drug resistant.This chapter reviews our information on the microbial colonization of neonatal feeding tubes. The range of organisms which have been recovered are wide, and while primarily bacterial, fungi such as Candida have also been found. The bacteria are principally Staphylococcus spp. and Enterobacteriaceae. The Enterobacteriaceae isolates are predominantly Enterobacter cancerogenus, Serratia marcescens, Enterobacter hormaechei, Escherichia coli and Klebsiella pneumoniae. Many of these isolates encode for antibiotic resistance; E. hormaechei (ceftazidine and cefotaxime) and S. marcescens strains (amoxicillin and co-amoxiclav).

Calcium carbonate precipitation by heterotrophic bacteria isolated from biofilms formed on deteriorated ignimbrite stones: influence of calcium on EPS production and biofilm formation by these isolates

Heterotrophic CaCO3-precipitating bacteria were isolated from biofilms on deteriorated ignimbrites, siliceous acidic rocks, from Morelia Cathedral (Mexico) and identified as Enterobacter cancerogenus (22e), Bacillus sp. (32a) and Bacillus subtilis (52g). In solid medium, 22e and 32a precipitated calcite and vaterite while 52g produced calcite. Urease activity was detected in these isolates and CaCO3 precipitation increased in the presence of urea in the liquid medium. In the presence of calcium, EPS production decreased in 22e and 32a and increased in 52g. Under laboratory conditions, ignimbrite colonization by these isolates only occurred in the presence of calcium and no CaCO3 was precipitated. Calcium may therefore be important for biofilm formation on stones. The importance of the type of stone, here a siliceous stone, on biological colonization is emphasized. This calcium effect has not been reported on calcareous materials. The importance of the effect of calcium on EPS production and biofilm formation is discussed in relation to other applications of CaCO3 precipitation by bacteria.

Identification of Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the VITEK MS system

This multicenter study evaluated the accuracy of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry identifications from the VITEK MS system (bioMérieux, Marcy l'Etoile, France) for Enterobacteriaceae typically encountered in the clinical laboratory. Enterobacteriaceae isolates (n = 965) representing 17 genera and 40 species were analyzed on the VITEK MS system (database v2.0), in accordance with the manufacturer's instructions. Colony growth (≤72 h) was applied directly to the target slide. Matrix solution (α-cyano-4-hydroxycinnamic acid) was added and allowed to dry before mass spectrometry analysis. On the basis of the confidence level, the VITEK MS system provided a species, genus only, or no identification for each isolate. The accuracy of the mass spectrometric identification was compared to 16S rRNA gene sequencing performed at MIDI Labs (Newark, DE). Supplemental phenotypic testing was performed at bioMérieux when necessary. The VITEK MS result agreed with the reference method identification for 96.7% of the 965 isolates tested, with 83.8% correct to the species level and 12.8% limited to a genus-level identification. There was no identification for 1.7% of the isolates. The VITEK MS system misidentified 7 isolates (0.7 %) as different genera. Three Pantoea agglomerans isolates were misidentified as Enterobacter spp. and single isolates of Enterobacter cancerogenus, Escherichia hermannii, Hafnia alvei, and Raoultella ornithinolytica were misidentified as Klebsiella oxytoca, Citrobacter koseri, Obesumbacterium proteus, and Enterobacter aerogenes, respectively. Eight isolates (0.8 %) were misidentified as a different species in the correct genus. The VITEK MS system provides reliable mass spectrometric identifications for Enterobacteriaceae.

Metabolite analysis on reductive biodegradation of reactive green 19 in Enterobacter cancerogenus bearing microbial fuel cell (MFC) and non-MFC cultures

The first-attempt study uncovered metabolic pathways of reactive green 19 (RG19) decolorization by Enterobacter cancerogenus BYm30 in single chamber membrane-less microbial fuel cell (SCML-MFC) and non-MFC cultures. The finding indicated that menaquinone from BYm30 might play as a redox mediator (or an electron-shuttling carrier) to stimulate dye decolorization and bioelectricity generation in MFC. Due to the formation of reduced organic sulfides from RG19 in MFC, color removal in MFC was significantly faster than non-MFC cultures. In addition, tertiary alcohols and phenols originally present in LB broth might be degraded by BYm30 under anaerobic condition regardless of MFC or non-MFC condition. Apparently, these novel findings could clarify the mechanism(s) behind reductive decolorization of RG19 taking place in MFC and non-MFC conditions for wastewater decolorization.

Biochemical and Molecular Diversity Analysis of Culturable Bacteria in Cotesia plutellae (Kurdjumov) (Hymenoptera: Braconidae), a Parasitoid of Diamondback Moth, Plutella xylostella (Linnaeus)

Geographical populations of Cotesia plutellae , a predominant endolarval parasitoid of the diamondback moth, Plutella xylostella (Linnaeus) were screened and analyzed for bacterial diversity. The culturable bacterial species were isolated and identified by sequence analysis of 16S rRNA gene. Eleven bacterial isolates were identified viz., Pseudomonas sp., Enterobacter cancerogenus, Bacillus spp., Pseudomonas putida, Pantoea agglomerans, Bacillus thuringiensis, Pantoea sp. and Bacillus cereus . The molecular characterization and phylogenetic analysis placed these phylotypes into two major classes i.e. Bacilli and Gamma proteobacteria. The evolutionary distance matrix (Pairwise distance) showed similarity between the sequences. The bacterial diversity observed was low in the different geographic populations. The nucleotide sequences were blasted and submitted to GenBank.

Metagenomic sequencing of the human gut microbiome before and after bariatric surgery in obese patients with type 2 diabetes: correlation with inflammatory and metabolic parameters

Roux-en-Y gastric bypass (RYGB) has become a prominent therapeutic option for long-term treatment of morbid obesity and type 2 diabetes mellitus (T2D). Cross talk and pathogenetic consequences of RYGB-induced profound effects on metabolism and gut microbiome are poorly understood. The aim of the present study therefore was to characterize intra-individual changes of gut microbial composition before and 3 months after RYGB by metagenomic sequencing in morbidly obese patients (body mass index (BMI)>40 kg m(-)(2)) with T2D. Subsequently, metagenomic data were correlated with clinical indices. Based on gene relative abundance profile, 1061 species, 729 genera, 44 phyla and 5127 KO (KEGG Orthology) were identified. Despite high diversity, bacteria could mostly be assigned to seven bacterial divisions. The overall metagenomic RYGB-induced shift was characterized by a reduction of Firmicutes and Bacteroidetes and an increase of Proteobacteria. Twenty-two microbial species and 11 genera were significantly altered by RYGB. Using principal component analysis, highly correlated species were assembled into two common components. Component 1 consisted of species that were mainly associated with BMI and C-reactive protein. This component was characterized by increased numbers of Proteobacterium Enterobacter cancerogenus and decreased Firmicutes Faecalibacterium prausnitzii and Coprococcus comes. Functional analysis of carbohydrate metabolism by KO revealed significant effects in 13 KOs assigned to phosphotransferase system. Spearmen's Rank correlation indicated an association of 10 species with plasma total- or low-density lipoprotein cholesterol, and 5 species with triglycerides. F. prausnitzii was directly correlated to fasting blood glucose. This is the first clinical demonstration of a profound and specific intra-individual modification of gut microbial composition by full metagenomic sequencing. A clear correlation exists of microbiome composition and gene function with an improvement in metabolic and inflammatory parameters. This will allow to develop new diagnostic and therapeutic strategies based on metagenomic sequencing of the human gut microbiome.

Enterobacter cancerogenus in Trauma

<i>Enterobacter cancerogenus</i> in Trauma