End Of 30th Day Research Articles

Anti-diabetic potential and safety profiles of Tephrosia purpurea on streptozotocin-nicotinamide induced diabetes in rats

Background: Tephrosia purpurea was traditionally used for the management of diabetes mellitus. Since this claim has not been investigated scientifically, the aim of this study was to evaluate the antidiabetic activity of Tephrosia purpurea extracts against STZ-Nicotinamide induced diabetes in rat. Methods: This preclinical study was done to evaluate the anti-hyperglycaemic activity of whole plant extract of Tephrosia purpurea in STZ- Nicotinamide induced diabetic rats, which produced a significant difference in blood glucose, lipid profile, renal and liver profile in comparison to untreated rats. In this study, the animals were divided into five groups and diabetes was induced by administering STZ-Nicotinamide and animals with blood sugar level >200 were enrolled in the study. Further the animals are grouped into Group I control (0.1% CMC), Group II, diabetic control and Group III reference control received glibenclamide. Group IV and V, diabetic rats treated with Tephrosia purpurea extract 200 mg/kg and 400 mg/kg respectively. All the test drugs were administered for 30 days. Results: In diabetic rats, treated with 200 and 400 mg/kg of Tephrosia purpurea blood glucose level significantly lowered on 10th day (p<0.05) and 5th day (p<0.01) respectively as compared to untreated rats. At the end of 30th day there is significant reduction in blood glucose treated with TP 400 mg/kg (p<0.001). Safety assessment shows the protective effect of TP (400 mg/kg) on lipid profile TC, TG (p<0.001), HDL (p<0.001), LDL (p<0.001) and VLDL (p<0.01). It also shows protective activity against AST (p<0.001), ALT (p<0.01), ALP (p<0.001) and Renal functions BUN (p<0.001), Creatinine (p<0.001). Conclusion: The anti-hyperglycaemic activity of Tephrosia purpurea is brought out in the study by its significant reduction in the blood glucose level. The safety and efficacy is established based on the protective effect of Tephrosia purpurea in lipid profile, renal and hepatic function of diabetic rats.

OIL EFFLUENT EXPOSURE INDUCED BIOCHEMICAL MODULATIONS IN SUBCELLULAR / TISSUES (CYTOSOL,MICROSOMES) OF FOOT, GILL AND DIGESTIVE GLANDS OF LAMELLIDENS MARGINALIS.

Sub-cellular fractions (cytosolic and microsomal) were prepared from the tissues (foot, gill and digestive gland) of freshwater mussel Lamellidens marginalis and were scrutinized to investigate the modulation of biochemical components (protein, carbohydrate and lipids) after exposure of sub-lethal concentrations of 1/4th (11.88 ppt) and 1/10th (8.55 ppt) of 96-hr LC 50 of oil effluent, and recovery period (depuration-without oil effluent) after seven days interval i.e., 1st, 8th, 15th 22nd and 30th days. At the end of 30th day, biochemical components in both exposure and depuration periods, were analysed from the tissues of mussels. The accumulation of oil effluent in the tissues gradually increased, when exposed to both sub-lethal concentrations of oil effluent and significantly and gradually decreased levels of protein, carbohydrate and lipid contents in the sub-cellular tissues of mussels were observed. During recovery period (without adding oil effluent), all depleted biochemical contents were recovered, increased, gradually, significantly from 30th day to Ist day (P<0.05) and one-way ANOVA showed progressive positive regression coefficient values (P<0.05) in all days. The enhanced and recovered levels of biochemical components in oil-exposed mussels demonstrate a well-established defense mechanism in mollusc, and this response offers the possibility of use as a biomarker for the early detection of oil pollution.

A comparative study of phenytoin dressing versus honey dressing in the treatment of cronic ulcers in tertiary care hospital

Background: Diabetic foot infections are the leading cause of hospitalization morbidity and mortality in diabetics worldwide In India it accounts for 20% of hospitalizations. honey has been investigated to treat ulcers like pressure ulcers, venous ulcers, diabetic ulcers, traumatic wounds and burns. Phenytoin is also now known to promote wound healing and can be an alternative to normal saline specifically in chronic wounds related to diabetes. The present study compares these two modalities in management of diabetic ulcers. Objective: To compare the efficacy of topical phenytoin vs topical honey in chronic diabetic ulcers in terms of rate of wound healing, granulation tissue formation and quality of graft bed for skin grafting. Methods: A comparative prospective study of 50 patients with chronic diabetic ulcers selected based on a predefined criteria divided into two groups: Group A (Phenytoin group) (n=25) and Group B (honey group) (n=25). Final wound area was measured on 30th day. Comparison between the wound size reduction among two groups done at end of 30th day. Outcome was measured in terms of wound reduction, granulation tissue formation and quality of bed for skin grafting between the two groups. Results: This study has shown faster rate of wound healing, better granulation tissue formation and quality graft bed for skin grafting in patients who received topical phenytoin dressing as compared to topical honey dressing.

COVID-19 outbreak reproduction number estimations and forecasting in Marche, Italy

BackgroundCOVID-19 disease is becoming a global pandemic and more than 200 countries were affected because of this disease. Italy is one of the countries is largely suffered with this virus outbreak, and about 180,000 cases (as of 20 April 2020) were registered which explains the large transmissibility and reproduction case numbers. ObjectiveIn this study, we considered the Marche region of Italy to compute different daily transmission rates (Rt) including five provinces in it. We also present forecasting of daily and cumulative incidences associated after the next thirty days. The Marche region is the 8th in terms of number of people infected in Italy and the first in terms of diffusion of the infection among the 4 regions of the center of Italy. MethodsEpidemic statistics were extracted from the national Italian Health Ministry website. We considered outbreak information where the first case registered in Marche with onset symptoms (26 February 2020) to the present date (20 April 2020). Adoption of incidence and projections with R statistics was done. ResultsThe median values of Rt for the five provinces of Pesaro and Urbano, Ancona, Fermo, Ascoli Piceno, and Macerata, was 2.492 (1.1–4.5), 2.162 (1.0–4.0), 1.512 (0.75–2.75), 1.141 (1.0–1.6), and 1.792 (1.0–3.5) with 95% of CI achieved. The projections at end of 30th day of the cumulative incidences 323 (95% CI), and daily incidences 45 (95% CI) could be possible. ConclusionsThis study highlights the knowledge of essential insights into the Marche region in particular to virus transmission dynamics, geographical characteristics of positive incidences, and the necessity of implementing mitigation procedures to fight against the COVID-19 outbreak.

Effect of Hemocare Syrup and Hemocare XT Tablets on Hemoglobin levels in iron deficiency anemia among women of reproductive age: A randomized, placebo controlled, open label trial

BackgroundIron deficiency anemia is one of the important public health problems in developing countries among the women of reproductive age group. Hemocare formulation plays a significant role in Iron deficiency anemia because of its Iron content. The present study aimed to investigate the effect of Hemocare Syrup and Hemocare XT Tablets compared with placebo on Hemoglobin Levels in Iron Deficiency Anemia among Women of Reproductive Age. MethodsA prospective, interventional, randomized, open label, placebo controlled trial has been conducted with 126 patients. Eligible patients randomly received either Placebo 100 mg or Hemocare XT Tablet 100 mg or 10 ml of Hemocare Syrup once daily in the same manner for 4 weeks. Hemoglobin values were documented at the end of 15th day, 21st day and 30th day. ResultsThe baseline hemoglobin values were compared with each follow up and statistically significant difference was found at the end of 30th day of Hemocare Syrup and Hemocare XT treatment (P < 0.05) but there was no significant difference observed in the placebo group. The study results revealed that 30 days treatment of Hemocare Syrup and Hemocare XT Tablets increases the Hemoglobin levels to 12.46 ± 0.44 g/dl and 12.90 ± 0.98 g/dl from 9.08 ± 2.54 g/dl and 9.68 ± 2.04 g/dl respectively. ConclusionHemocare Syrup and Hemocare XT Tablets treatment showed clinically significant improvement in hemoglobin levels from the baseline and placebo group.

Kadmiyum ile Oksidatif Strese Maruz Kalan Sıçanların Beyin Dokusunda Astaksantinin Etkisi;Biyokimyasal, Histopatolojik Değerlendirme

Aim of this study is to evaluate protective impact of Astaxanthin (AST) on rats with experimentalbrain injury induced with Cadmium (Cd). 32 male Wistar albino rats were divided into four groups as Control,Cadmium, Astaxanthin (AST), Cadmium (Cd)+Astaxanthin (AST). Rat brain tissues were obtained at the endof 30th day. Malondialdehyde (MDA), glutathione (GSH) levels and superoxide dismutase (SOD) enzymeactivities were measured in brain homogenates and histopathological examination was performed. MDA levelswere improvement in cadmium administered group (p&amp;lt;0.01) as well as Cd+AST administered group (p&amp;lt;0.05)compared to control group. In addition a substantial reduction Cd+AST group was observed compared to cadmiumadministered group (p&amp;lt;0.01). GSH level shows a decrease in Cd and Cd+AST groups compared to control (p&amp;lt;0.05).SOD enzyme activity was found significantly lower in Cd and Cd+AST groups compared to control (p&amp;lt;0.01). Inaddition, increase of SOD in Cd+AST group compared to cadmium group was also found significant (p&amp;lt;0.05).Histopathological findings in the cerebral cortex and hippocampus were different between groups. In the controland AST administered groups, normal histological structure was observed in the brain, while severe lesions wereseen in the Cd administered group and in the Cd+AST group only mild degenerative lesions were observed.As a result, elevated MDA level due to Cd administration was attenuated with AST administration. Decreased GSHlevel and SOD enzyme activity due to Cd administration was increased with AST administration. In addition, ASTadministration decreased histopathological lesions. Consequently, it is thought that AST may be used for protectionagainst brain oxidative damage due to Cd.

Enhanced Biogas Production from Co-digestion of Intestine Waste from Slaughterhouse and Food Waste

The presence of higher protein and lipids contents of the intestine waste from slaughterhouse affects the anaerobic digestion (AD) process due to volatile fatty acids accumulation and ammonia toxicity. The aim of the present batch reactor study was to evaluate the effect of the mixing ratio on anaerobic co-digestion of intestine waste from the slaughterhouse (IWS) and carbon-rich food waste (FW) to enhance the biogas production and to optimize the C/N ratio for improving the efficiency and better performance of AD. Biogas productions were estimated for various mixing ratios on volatile solid (VS) basis (IWS/FW: 1:1, 1:2, 1:3, 1:4, and 1:5) keeping the inoculum to substrate ratio maintained at 0.5 as per German method VDI 4630. On the basis of the present study, the biogas productions of different mixing ratios at the end of 30th day were found to be on the order of 0.43 (1:2) > 0.39 (1:3) > 0.38 (1:1) > 0.36 (FW) > 0.35 (1:4 and 1:5) > 0.18 (IWS) L/g of VS added. Similarly, the VS removal efficiencies wer...

Pregabalin does not affect fracture healing adversely.

This study aims to assess the radiographic, histological, and biomechanical effects of pregabalin on fracture healing in a rat model of femur fracture. A total of 32 female Wistar-Albino rats (mean age 12±1 weeks; mean body weight 236±12 grams) were randomized into four groups with eight rats in each group. Groups A and B were the control groups whereas C and D were the treatment groups. Drugs were delivered by oral gavage. Radiographic, histological and biomechanical evaluations were performed after sacrificing the rats in groups A and C on 15th postoperative day and the rats in groups B and D on 30th postoperative day. Although radiographic results were better in groups A and B than groups C and D at the end of 15th day, no significant difference was detected at the end of 30th day. No statistically significant differences were observed between the groups on 15th and 30th days in terms of histological or biomechanical evaluation. Administration of pregabalin did not affect fracture healing process adversely in rats.

Functionalized polycaprolactam as an active food package for antibiofilm activity and extended shelf life

Papain is covalently crosslinked on polycaprolactam and tested as a wrapper for packaging cottage cheese, against E. coli biofilm. The bacterial count on neat polycaprolactam (NP) was 50×106/ml on the 5th day which dramatically increased to 300×106colony forming units (CFU)/ml by the end of 30th day. The corresponding CFU/ml on papain functionalized polycaprolactam (FP) was 10×102 on 5th day and 20×102 by the end of 30th day. Fourier transform infrared spectroscopic (FTIR) analysis of biofilm on NP showed the presence of polysaccharide, protein, lipid and metabolites which was three times reduced on FP. FT Raman spectroscopy showed the effect of papain on functional groups such as hydroxyl, amino, carbonyl, phosphoryl and aliphatic, leading to the inhibition of the biofilm. Motility, hydrophobicity and zeta potential of E. coli on NP and FP were 10.67 and 5.65μm/s/V/cm; 88 and 20%; 8.93±2.09 and 2.65±0.52mV respectively, thereby decreasing the biofilm forming ability of E. coli.

Effects of Commonly Consumed Beverages on Surface Roughness and Color Stability of the Nano, Microhybrid and Hybrid Composite Resins: An in vitro Study

Consumption of certain beverages may affect the esthetic and physical properties of the resin composite, thereby undermining the quality of restorations. To analyze the effect of three beverages (cola, coffee, tea) on color stability and surface roughness of three different types of resin composites at various time intervals in vitro. Nano, microhybrid and hybrid resin composites were used. Each material was randomly divided into four equal subgroups of 10 samples each according to the beverages used (cola, coffee, tea, distilled water). The samples were immersed in each beverage for 1, 15 and 30 days. Surface roughness and color changes measurements were noted at the baseline-the first, fifteenth and thirteenth day. It was found that nanoresin composite followed by microhybrid and hybrid showed least surface roughness and color change. The Coke beverage subgroup showed more surface roughness and the subgroup coffee has shown more color changes with respect to other subgroups. All specimens showed discoloration after completion of the test period which was visually perceptible and clinically unacceptable. At the end of 30th day, among the materials, nanofilled composite resin showed comparatively less surface roughness and color change than microhybrid and hybrid composite resins.

Subcellular/tissue distribution and responses to oil exposure of the cytochrome P450-dependent monooxygenase system and glutathione S-transferase in freshwater prawns (Macrobrachium malcolmsonii, M. lamarrei lamarrei)

Subcellular fractions (mitochondrial, cytosolic and microsomal) prepared from the tissues (hepatopancreas, muscle and gill) of freshwater prawns Macrobrachium malcolmsonii and Macrobrachium lamarrei lamarrei were scrutinized to investigate the presence of mixed function oxygenase (MFO) and conjugating enzymes (glutathione-S-transferase, GST). Cytochrome P450 (CYP) and other components (cytochrome b(5); NADPH-cytochrome c (CYP) reductase and NADH-cytochrome c-reductase activities) of the MFO system were predominantly present in the hepatic microsomal fraction of M. malcolmsonii and M. lamarrei lamarrei. The results are in agreement with the notion that monooxygenase system is mainly membrane bound in the endoplasmic reticulum, and that the hepatopancreas is the major metabolic tissue for production of biotransformation enzymes in crustaceans. Further, the prawns were exposed to two sublethal (0.9 ppt (parts per thousand) and 2.3 ppt) concentrations of oil effluent. At the end of 30th day, hydrocarbons and detoxifying enzymes were analysed in the hepatopancreas. The accumulations of hydrocarbon in the tissues gradually increased when exposed to sublethal concentrations of oil effluent and were associated with significantly enhanced levels of cytochrome P450 (180.6+/-6.34 pmol mg(-1) protein (P<0.05 versus control, 136.5+/-7.1 pmol mg(-1) protein) for 2.3 ppt and 305.6+/-8.5 pmol mg(-1) protein (P<0.001 versus control, 132.3+/-6.8 pmol mg(-1) protein] for 0.9 ppt of oil exposed M. malcolmsonii; 150+/-6.5 pmol mg(-1 )protein (P<0.01 versus control, 84.6+/-5.2 pmol mg(-1) protein) for 2.3 ppt and 175+/-5.5 pmol mg(-1) protein (P<0.01 versus control, 87.6+/-5.4 pmol mg(-1) protein) for 0.9 ppt of oil exposed M. lamarrei lamarrei), NADPH cytochrome c-reductase activity (14.7+/-0.6 nmol min(-1 )mg(-1) protein (P<0.05 versus control, 6.8+/-0.55 nmol min(-1 )mg(-1) protein) for 2.3 ppt and 12.1+/-0.45 nmol min(-1 )mg(-1) protein (P<0.01 versus control, 6.9+/-0.42 nmol min(-1 )mg(-1) protein) for 0.9 ppt of oil exposed M. malcolmsonii; 12.5+/-0.31 nmol min(-1 )mg(-1) protein (P<0.001 versus control, 4.6+/-0.45 nmol min(-1 )mg(-1) protein) for 2.3 ppt and 9.6+/-0.32 nmol min(-1 )mg(-1) protein (P<0.01 versus control, 4.9+/-0.41 nmol min(-1 )mg(-1) protein) for 0.9 ppt of oil exposed M. lamarrei lamarrei) and cytochrome b(5 )(124.8+/-3.73 pmol mg(-1) protein (P<0.01 versus control, 76.8+/-4.2 pmol mg(-1) protein) for 2.3 ppt and 115.3+/-3.86 pmol mg(-1) protein (P<0.01 versus control, 76.4+/-4.25 pmol mg(-1 )protein) for 0.9 ppt of oil exposed M. malcolmsonii and 110+/-3.11 pmol mg(-1) protein (P<0.01 versus control, 63.7+/-3.24 pmol mg(-1 )protein) for 2.3 ppt and 95.3+/-2.63 pmol mg(-1) protein (P<0.01 versus control, 61.4+/-2.82 pmol mg(-1) protein) for 0.9 ppt of oil exposed M. lamarrei lamarrei). The enhanced levels of biotransformation enzymes in oil-exposed prawns demonstrate a well-established detoxifying mechanism in crustaceans, and the response offers the possibility of use as a biomarker for the early detection of oil pollution.