Background: In developed countries, diabetic kidney disease (DKD) is the major cause of end-stage kidney disease (ESKD). Microalbuminuria and elevation of serum creatinine are observed only in the later stages of disease. Aim of this study is to evaluate the association of Cystatin C with Diabetic Nephropathy in normoalbuminuric type 2 Diabetes Mellitus indiviaduals with GFR <90ml/min/1.73m2 and >60ml/min/1.73m2 . Material & Method: The present observational cross-sectional study was conducted among the patients with Type 2 diabetes mellitus. Patients' serum creatinine, serum cystatin C, urine 24 hr creatinine clearance, and urine albumin creatinine ratio (ACR) levels were assessed. The eGFR was calculated and compared with the parameters. The diagnostic accuracy was calculated by AUC analysis, Pearson Correlation were measured. The p-value of <0.05 was considered statistically signicant. In present study total Results: of 100 patients fullling inclusion criteria are included after obtaining the informed consent. The mean age of patients was found to be 49.54±8.31yrs of age. Cystatin C showing a signicant diagnostic strength in detecting the presence of the diabetic nephropathy with AUC of 0.91, p<0.05. The eGFR showing the strength of detection of the diabetic nephropathy, we found CKDEPI cr-cys eGFR was showing the strong predictability with AUC of 0.908, compared to other equations. The strength of association of HbA1c was stronger with the GFR derived from CKDEPI cr-cys. The study found the signicant efcacy of Conclusion: the serum Cystatin C in comparison to the serum creatinine clearance in diagnosis of the renal dysfunction in patients of diabetes mellitus. Cystatin C was signicantly associated with nephropathy in normoalbuminuric type 2 Diabetes Mellitus indiviaduals with GFR <90ml/min/1.73m2 and >60ml/min/1.73m2. Our study suggest that Cystatin C is a useful, non-invasive, practical tool for renal involvement in the course of normoalbuminuric Diabetes Mellitus individuals