Electron Spectroscopy Methods Research Articles

Salt Engineering of Aripiprazole with Polycarboxylic Acids to Improve Physicochemical Properties.

Aripiprazole (APZ) has poor physicochemical properties and bitter taste. The current study aimed to prepare salts of APZ with polycarboxylic acids (citric, malic, and tartaric acids) to improve physicochemical properties and impart sour taste to the drug. The salts were prepared by solubilization-crystallization method, and characterized by electron microscopic, spectroscopic, diffractometry, and thermal methods. The salts were assessed for pH solubility, pH-stability, dissolution, and solid-state stability. Fourier transformed infrared, X-ray powder diffraction, and differential scanning calorimetry data indicated formation of new solid phases. APZ and the salts exhibited pH-dependent solubility. The pH solubility curve shape was inverted "V," inverted "W," and inverted "U" for APZ, APZ-Citrate, and APZ-Malate and APZ-Tartrate, respectively. Compared to APZ, the solubility of salts at pH4, 5, and 6 was 3.6-7.1, 23.9-31.5, and 143.4-373.3 folds of APZ. Increase in solubility in water by citrate, malate, and tartrate salts was 5562.8, 21,284.7, and 22,846.7 folds of APZ. The salt formation also leads to an increase in rate and extent of dissolution. The dissolution extent was 3.5 ± 0.5, 71.3 ± 1.2, 80.1 ± 6.2, and 86.1 ± 1.1% for APZ, APZ-Citrate, APZ-Malate, and APZ-Tartrate, respectively. Liquid and solid-state stabilities of the salts were comparable to APZ. In conclusion, salts of APZ with polycarboxylic acids improved solubility, and dissolution, and impart sour taste, which may improve palatability of the drug.

Electrosynthesis of Silver Nanocomposites Based on N-Vinylimidazole and N-Vinylpyrrolidone

Silver nanocomposites and nanocomposite coatings based on copolymers of N-vinylimidazole and N-vinylpyrrolidone are synthesized in water and water-ethanol media on electrodes of pure iron and steel by an electrochemical technique. The composition and structure of nanocomposites is confirmed by the methods of electron and IR spectroscopy, X-ray diffraction analysis, transmission electron microscopy, thermogravimetry, etc. The solubility of nanocomposites is shown to depend on the concentration of silver particles in the (co)polymer matrix. The thermal decomposition of nanocomposites proceeds in stages.

Experimental studies of optical properties of hard tissues of anterior teeth and modern synthetic filling materials

The article data on the study of reflection spectra of hard tooth tissues and dental restoration materials are presented. The method of investigation of dental hard tissues and dental restoration materials — the method of diffuse reflection electron spectroscopy is defined. The relevance of the work, since a high level of restoration aesthetics in therapeutic dentistry is important is shown. Reflection spectra of the studied samples on an automated spectrofluorometer “SDL-2” were performed. Spectral dependences of the reflection coefficient of enamel and dentin sections, as well as intact teeth of patients of different ages, were obtained. The spectral dependences of the reflection coefficient of various dental restoration materials were analyzed.

Study of the Optical Spectra of 4-Hydroxy-3-Methoxibenzoic Acid

Using electronic spectroscopy and quantum chemistry methods, the electronic spectra of vanillic acid in various solvents are interpreted. In the ground electronic state, four protolytic forms of vanillic acid in water were detected: anionic, dianionic, cationic, and neutral. In water, vanillin and isovanillin in the ground state have the same spectral characteristics: the line position and intensity in their absorption spectra coincide. The absorption and fluorescence bands of vanillic acid shift toward shorter wavelengths compared to vanillin and isovanillin. The values of the minimum electrostatic potential indicate that vanillic acid has the deepest minimum in the region of the carbonyl oxygen atom. The study of the fluorescence spectra showed that the radiative properties of the ionic forms of vanillic acid are different. The data of quantum chemical calculations indicate that electronic transitions in the molecule are formed with the participation of charge transfer from the phenyl part of the molecule to the oxygen atoms of the methoxy and carbonyl groups. The presence of OH, OCH3, and carbonyl group in the structure of vanillic acid leads to the existence of a dianionic form, both in the ground and electronically excited states.

Chemical and structural properties of reduced graphene oxide\u2014dependence on the reducing agent

Graphene oxide (GO) prepared from graphite powder using a modified Hummers method and reduced graphene oxide (rGO) obtained from GO using different reductants, i.e., sodium borohydride, hydrazine, formaldehyde, sodium hydroxide and L-ascorbic acid, were investigated using transmission electron microscopy, X-ray diffraction, Raman, infrared and electron spectroscopic methods. The GO and rGOs’ stacking nanostructure (flake) size (height x diameter), interlayer distance, average number of layers, distance between defects, elementary composition, content of oxygen groups, C sp3 and vacancy defects were determined. Different reductants applied to GO led to modification of carbon to oxygen ratio, carbon lattice (vacancy) and C sp3 defects with various in-depth distribution of C sp3 due to oxygen group reduction proceeding as competing processes at different rates between interstitial layers and in planes. The reduction using sodium borohydride and hydrazine in contrary to other reductants results in a larger content of vacancy defects than in GO. The thinnest flakes rGO obtained using sodium borohydride reductant exhibits the largest content of vacancy, C sp3 defects and hydroxyl group accompanied by the smallest content of epoxy, carboxyl and carbonyl groups due to a mechanism of carbonyl and carboxyl group reduction to hydroxyl groups. This rGO similar diameter to GO seems to result from a predominant reduction rate between the interstitial layers. The thicker flakes of a smaller diameter than in GO are obtained in rGOs prepared using remaining reductants and result from a higher rate of reduction of in plane defects.

Formation of Fe<sub>2</sub>Si Wetting Coating on Si(001) 2×1 and Growth of a Stable Fe Nanolayer: AES and EELS Study

The structural-phase state of two-dimensional (d = 1 - 3 ML) coatings before and after annealing and that of Fe nanolayers (d = 4 - 10 ML) were investigated on Si (001)2×1 by electron spectroscopy methods of Auger-electrons and energy losses. The room (30 °С) and lowed (≤ 1250 °С) temperatures of the Si (001) substrate and Fe vapor, respectively, were used during Fe deposition. This study showed the following. An ordered two-dimensional 1×1 phase of Fe stable up to 600 °C and then a Fe2Si wetting coating stable at 250 °C form near the thickness 1 ML and 3 ML, respectively. Fe deposition on this Fe2Si coating leads to the one-after-another formation of the Fe3Si, the Si-in-Fe solution, and then a stable up to 250 °C Fe nanofilm with segregated Si.

Two-Stream Model of Monoenergetic Electron-Beam Backscattering: Application to Problems of the Diagnostics of Thin-Film Structures

The energy spectra of electrons reflected from individual layers located inside a multilayer thin-film target are calculated as applied to problems of the backscattered electron spectroscopy method (BESM). The structure of analytical expressions describing the distribution of the electron-backscattering coefficient over the sample depth is refined. A formula for the two-dimensional polar-angle distribution of backscattered electrons after their exit from a thin-film target with a given thickness is obtained. An algorithm for calculations required to construct the energy distributions of backscattered electrons is developed for a given angular position of the input diaphragm of an analyzer spectrometer. This makes it possible to bring our calculations closer to the actual conditions for recording backscattered electrons. The results of comprehensive verification of the obtained relations based on comparing our model calculations of the energy distributions of backscattered electrons with the experimental spectra of free films, bulk samples, and a multilayer thin-film sample on a massive substrate are given.

Atmospheric Corrosion Influence in the Manufactured Processes of a Seafood Industry Located in Ensenada, Mexico

The atmospheric corrosion presented in indoors of a seafood industry, generated aggressive environments and with this deterioration in steel cans used to can sardine and tuna food, which were corroded in the manufacturing processes of this industry evaluated. Even using metal containers with a special coating to can tuna and sardines and with the exposition of aggressive environments the deterioration of these cans was made, before being used for the container of tuna and sardines in the manufacturing areas. The air pollutants as sulfurs principally, which generated the aggressive environments penetrated through cracks and air conditioning systems to indoors of the seafood industry evaluated in Ensenada, promoting the corrosion process originated. The occurrence of corrosion in the seafood industry evaluated, increased the costs of the manufacturing processes for the necessity of takeoff the deterioration of steel cans corroded used to pack tuna and sardine in 45% of the steel cans received of an industry of United States, and around 35% of the steel cans used had to be replaced. This represented a decrease in competitiveness in this industrial company evaluated, presenting economic losses, caused by presence the air pollution of Ensenada, which generated the deterioration in steel cans, being originated principally sulfurs of cars and chloride ions of sea breeze. Recently in this city of the northwest of the Mexican Republic, the indices of air pollutants emitted by the traffic vehicle, are increased and overpass the air quality standards (AQS), determined by the Secretaria de Medio Ambiente y Recursos Naturales (SEMARNAT-Mexico) and the Environmental Protection Agency (EPA-USA). For this reason an analysis of the atmospheric corrosion in indoors of a seafood industry located in Ensenada city, was made to identify the aggressive agents that reacted with the metallic surface of steel cans used to can tuna and sardine, with micro evaluation with technique of Scanning Electron Microscopy (SEM) and nano analysis with Auger Electron Spectroscopy (AES) method. The investigation was made in 2019.

STRUCTURAL-GROUP COMPOSITION AND GROWTH-REGULATING ACTIVITY OF D-GLUCOSE–M-AMINODENZOIC ACID CONDENSATION PRODUCTS

Structural-group composition is studied, mechanisms of formation are proposed and assessment of plant growth activity of condensation products in D-glucose–m-aminobenzoic acid (m-ABA) system is carried out. Solid product of complete reaction system, formed from initial components as a result of complex reaction combination is fractionated with water, nondialysable component is isolated from water-soluble fraction, studied by methods of elemental analysis, electronic and vibrational spectroscopy, as well as tested as growth regulating agent. According to IR-Fourier spectroscopy, undialysable water-soluble products are characterized by structural-group compositions close to natural humic substances: mainly aliphatic backbone including C=C, C=O, C–O, OH-functions and bounded arylamine fragments (m-ABA). The profile of the electron spectra confirms the presence of sufficiently long chromophores in the structure. Such functionalization provided the necessary ratio in the product structure of the active groups and the aromatic component for the development of humic products growth activity at low concentrations due to optimal polar-hydrophobic interactions at supramolecular association level, which was confirmed experimentally by the evaluation of cucumber seeds growth indices of the "Nezhinsky" variety. Additional spectral studies of seed seedlings allowed to justify the mumbling effect of synthesized products, the maximum effect of which was observed in the area of low (0.0008%) solutions concentrations, which is also characteristic of natural humate’s stimulating influence. Further studies are planned in the direction of studying the effect of synthesized products on individual phases of plant development, as well as estimation of their ecotoxicity and biodegradation ability. The results obtained supposed to be the basis of technological scheme for production of processed humin-like products on an industrial scale.

A potent drug candidature of Cu(II) pyrazino[2,3‐f][1,10]phenanthroline complexes with bioactive ligands: synthesis, crystal structures, biomolecular interactions, radical scavenging and cytotoxicities

A novel ternary copper(II) complexes, - [Cu(py-phen)(asn)(NO3)(H2O)] (1) and [Cu(py-phen)(trp)(H2O)]NO3 (2)- (py-phen: pyrazino[2,3‐f][1,10]phenanthroline, asn: asparagine, trp: tryptophan), have been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complexes 1 and 2 with CT-DNA has been investigated by absorption spectral titration, EB and Hoechst 33258 displacement assay. The interaction between the complexes 1 and 2 and BSA was investigated by electronic absorption and fluorescence spectroscopy methods. The experimental outcomes indicate that the fluorescence quenching mechanism between the complexes 1 and 2 and BSA is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (ΔG, ΔH, ΔS) of BSA + complex systems were determined at different temperatures. The binding distance between the complexes 1 and 2 and BSA was calculated according to FRET. The effect of the complexes 1 and 2 on the conformation of BSA was also examined using synchronous, two dimensional (2D) and three dimensional (3D) fluorescence spectroscopy. Radical scavenging activity of the complex was determined in terms of EC50, using the DPPH and H2O2 method. The anticancer activities of the complexes 1 and 2 were investigated using an XTT assay against three cancer cell lines (MCF-7, Caco-2 and A549) and non-tumor cell line (BEAS-2B). Communicated by Ramaswamy H. Sarma

Investigation on water soluble copper(II) mono-anionic glutamate complexes with planar aromatic ligands: synthesis, crystal structures, biomacromolecular interactions and radical scavenging activities

Two water soluble copper(II) mono-anionic glutamatecomplexes -[Cu(phen)(glu)(H2O)]NO3·H2O (1) and [Cu(py-phen)(glu)(H2O)]NO3·H2O (2) - (phen: 1,10-phenanthroline, py-phen: pyrazino[2,3-f][1,10]phenanthroline, glu: glutamic acid), have been synthesized and characterized by CHN analysis, ESI-MS, FTIR and single-crystal X-ray diffraction techniques. Interaction of the complex with biomacromolecules {calf thymus DNA (CT-DNA) and bovine serum albumin (BSA)} has been investigated by electronic absorption and fluorescence spectroscopy methods. The experimental outcomes displays that the complexes 1 and 2 bind to CT-DNA by means of a moderate intercalation mode. Furthermore, the fluorescence quenching mechanism between BSA and the complexes 1 and 2 is a static quenching process. The Stern-Volmer constants, binding constants, binding sites and the corresponding thermodynamic parameters (ΔG, ΔH, ΔS) of BSA+ the complexes 1 and 2 systems were determined at different temperatures. The binding distance between BSA and the complexes 1 and 2 was calculated according to Förster non-radiation energy transfer theory (FRET). The effect of the complexes 1 and 2 on the conformation of BSA was also examined using synchronous, two dimensional (2D) and three dimensional (3D) fluorescence spectroscopy. Radical scavenging activity of the complexes 1 and 2 was determined in terms of IC50, using the DPPH and H2O2 method. In biomacromolecules interactions and radical activity studies, the complexes 1 and 2 were found to give good results.

Rhodium(III) complexes of 1-Alkyl-2-{(o-thioalkyl) phenylazo}imidazoles: synthesis, structure, spectral characterization, DNA binding study and DFT calculation

The Rh(III) complexes of 1-alkyl-2-{(o-thioalkyl)phenylazo}imidazole (SRaaiNR′, 1; R = R′= Me (a); R = Me, R′= Et (b); R = Et, R′= Me (c); R = R′= Et (d)), [Rh(SRaaiNR′)(PPh3)Cl2](ClO4) (2) have been synthesized. The complexes have been characterized by physicochemical and spectroscopic methods. The single-crystal X-ray diffraction study authenticates the structure of [Rh(SMeaaiNEt)(PPh3)Cl2](ClO4) (2b). The DNA binding ability of the complexes has been investigated by electronic absorption and fluorescence spectroscopic methods. Density functional theory computation technique has been used to enlighten the electronic structures and their spectral properties. 1-Alkyl-2-{(o-thioalkyl)phenylazo}imidazole complexes of Rh(III), [Rh(SRaaiNR′)(PPh3)Cl2](ClO4)](2) are synthesized and characterized. [Rh(SMeaaiNEt)(PPh3)Cl2](ClO4) (2b) is characterized by single-crystal X-ray diffraction study. Ligand acts as tridentate N(imidazole), N(azo) and S(thioether) donor centers. The DNA binding ability of the complexes is investigated by electronic absorption and fluorescence spectroscopic methods. The electronic structure and observed electronic transitions are interpreted by DFT and TDDFT computation.

Formation of humic substances in the reaction of D-glucose with p-toluidine in anhydrous ethanol

The aim of the study was to investigate the processes of formation and structural group composition of the products formed during the reaction of D-glucose with p-toluidine in anhydrous ethanol using electronic and vibrational spectroscopy methods. The evolution of the profiles of the electronic spectra of working solutions shows the formation of a chromophore system: clear peaks at 380 and 440 nm are already present at a reaction time of 60 min. The appearance of an additional maximum at 520 nm (90, 120 min) likely corresponds to the formation of chromophores in condensed structures, as confirmed by a decrease in the values of the E 4 /Е 6 indices over time. The dynamics of the transformation of structural elements is also clearly seen in the analysis of IR spectra: as the reactions proceed, the CH aliphatic component of the structure decreases, while the aromatisation and functionalisation of products by chromophore groups increases. For products obtained between 90 and 120 min, a clear band at 1656 cm -1 , attributed to stretching vibrations of multiple bonds in conjugated C=C – C=O systems and azomethine fragments, indicates the of condensation reactions. In the spectra of insoluble solid products fractionated by water, the intensity of the bands at 1656 and 1190 cm -1 is significantly reduced; this is possibly due to the transfer of some of the coloured low molecular weight products to the aqueous phase. A comparison of the IR Fourier transform spectra of the final solid products with the spectra of natural and synthetic humic-like substances indicates their similar structural group composition. The intensity of the bands at 1620, 1508 and 815 cm -1 indicates a significant contribution of the aromatic component to the structure of the products of water-insoluble fractions; the water-soluble fractions mainly include aliphatic oxygen-containing structures resulting from the oxidative destruction of the initial carbohydrate. The synthesised products seem promising as convenient plant growth regulators with controlled release of the active substance.

Experimental determination of electron attenuation lengths in complex materials by means of epitaxial film growth: Advantages and challenges

Accurate electron attenuation lengths are of critical importance in using electron spectroscopic methods to quantitatively characterize complex materials. Here, the authors show that analysis of core-level and valence-band x-ray photoelectron spectra excited with monochromatic AlKα x-rays from the substrate and measured as a function of film thickness can be used to determine electron attenuation lengths in epitaxial SrTiO3 films on Ge(001). Closely lattice-matched epitaxial heterojunctions are ideal systems for determining attenuation lengths provided the films grow in a layer-by-layer fashion, leading to atomically flat surfaces, and the buried interfaces are atomically abrupt. In principle, either the rate of attenuation of substrate peak intensities or the rate of increase of film peak intensities can be used for this purpose. However, the authors find that structural nonuniformities in the films reduce the accuracy of electron attenuation lengths determined from photoelectrons that originate within the films. A more reliable source of information is found in photoelectrons from the substrate which traverse the film. By using the energy dependence of calculated electron attenuation lengths from the NIST database in combination with Ge 3d core and Ge-derived valence-band intensities, the authors determine electron attenuation length as a function of kinetic energy for SrTiO3.

Fabrication of highly transparent Er3+, Yb3+:Y2O3 ceramics with La2O3/ZrO2 as sintering additives and the near-infrared and upconversion luminescence properties

Abstract Highly transparent Er3+, Yb3+:Y2O3 ceramics were fabricated by pressureless vacuum sintering method using La2O3 and ZrO2 as compound sintering additives. The microstructure, optical transmittance, upconversion and near-infrared spectral properties of the ceramics were investigated by X-ray diffraction, scanning electron microscopy, energy-dispersive spectrometry and spectroscopic analysis methods. The sintered ceramics displayed a pure cubic Y2O3 phase structure and a nearly pore-free microstructure with homogeneous grain morphology and grain-size distribution. The highest transmittance of Er3+, Yb3+:Y2O3 ceramics sintered at 1800 °C for 14 h with 9 at% of La2O3 and 3 at% of ZrO2 as compound additives was more than 82% in the range of 380–3000 nm. Under 980 nm wavelength excitation, the ceramics exhibited intense blue, green and red upconversion emission, and broad-bandwidth near-infrared emission centered at 1536 nm, which gives it the potential to act as a high-performance solid-state 1.54 μm near-infrared and upconversion laser gain media.

Гибридная система «слоистый силикат магния – хлорин е6 13(1),15(2),17(3)-N,N',N''-(2-гидроксиэтил)триамид»

Matrices based on layered silicates have a set of useful properties, in particular, they are able to increase the stability and photosensitizing ability of porphyrins. Hybrid systems “layered silicate - porphyrin (metal porphyrin)” are promising as active components of systems for light collecting and artificial photosynthesis, catalysts, photocatalysts. The hybrid systems with content of 1.4∙10-6 and 4.2∙10-6 mol of the chlorin e6 13(1),15(2),17(3)-N,N',N''-(2-hydroxyethyl)triamide per 1 g of layered magnesium silicate was obtained by heat treatment method of a synthetic layered magnesium silicate of hectorite composition when treated with an aqueous-alcoholic solution of organic component. The molar ratio of the starting components is: Mg(OH)2 : SiO2 : LiF = 1 : 1.51 : 0.25 : chlorin e6 derivative = 1 : 1.51 : 0.25 : (19.93∙10-5 – 59.58∙10-5). Using dynamic light scattering and laser Doppler electrophoresis, it was shown that the introduction of chlorin e6 13(1),15(2),17 (3)-N,N',N''- (2-hydroxyethyl) triamide leads to the growth of hybrid particles (162 ± 3 nm) and a decrease in their aggregative stability (-30 ± 1 mV), compared with the initial layered magnesium silicate (133 ± 1 nm, -33 ± 1 mV). In this case, the introduction of more hydrophilic chlorin e6 13(1),15(2),17(3)-N,N',N''-(2-hydroxyethyl)triamidе, in the molecule of which there are three fragments of ethanolamine, allows to obtain smaller hybrid particles (162 ± 3 nm), compared with hybrid particles (248 ± 6 nm) formed by the action of chlorin e6 13(1),17(3)-N,N'-(2-hydroxyethyl)diamide15(2)-methyl ether, in the molecule of which there are two fragments of ethanolamine. Using the methods of X-ray phase analysis and electron spectroscopy in the UV-visible region, it was found that individual molecules of chlorin e6 13(1),15(2),17(3)-N,N',N''-(2-hydroxyethyl)triamide were sorbed on the surface of silicate particles due to the electrostatic interaction between the protonated molecules of the macrocycle and the ionized hydroxyl groups of magnesium silicate. It was shown that the higher hydrophilicity of chlorin e6 13(1),15(2),17(3)-N,N',N''-(2-hydroxyethyl)triamide, compared with chlorin e6 13(1),17(3)-N,N'-(2-hydroxyethyl)diamide15(2)-methyl ether did not contribute to the intercalation of the organic component into the interlayer space of layered magnesium silicate under the conditions of a water-alcohol medium.

Predicting the photoresponse of soot nuclei: Spectroscopic characteristics of aromatic aggregates containing five-membered rings

Establishing the mechanism for soot nucleation will require in situ experimental characterization of the identity and intermolecular interactions of the initial precursors, and electronic spectroscopy methods have the potential to do both. However, the optical response of polycyclic aromatic hydrocarbon (PAH) dimers and complexes differs significantly from that of the constituent monomers, and studies of soot precursor complexes have largely been limited to PAHs containing only six-membered aromatic rings. Hydrocarbons containing unsaturated five-membered rings are also present in high concentration in flames, and the photoresponse of complexes containing five-membered rings has not yet been examined. In this work, we elucidate the spectroscopic properties of small hydrocarbon complexes containing cyclopentadienyl groups or cyclopenta-fused groups, calculating ground- and excited-state binding energies, optimal excited-state geometries, repulsion energies, and fluorescence emission wavelengths. We show that excited-state distortion of cyclopentadienyl groups allows strong binding and low-energy fluorescence emission compared to similar-sized PAHs, and that a cyclopenta-fused group dramatically lowers the absorption and emission energies for acenaphthylene, dominating excited-state noncovalent interactions—findings that could shed light on the complex electronic properties of flames.

Cesium with Residual Oxygen on a Cylindrical Tungsten Polycrystal

The chemical states and changes in the work function of a heterogeneous multilevel film have been determined during the adsorption of cesium at 295 K on a cylindrical gas-phase polycrystalline tungsten coating (30 μm) of Nb–1%Zr alloy with a minimum concentration of residual oxygen using low-energy threshold electron spectroscopy and contact potential difference methods. The chemical states and the change in the work function of this film have been found during successive heating up to 1875 K.

Modification of the Surface Properties of Free Si–Cu Films by Implantation of Active Metal Ions

Using the methods of Auger electron spectroscopy, electron energy loss spectroscopy, and UV photoelectron spectroscopy, the influence of Ba+ ion implantation on the composition, crystal structure, and electron configuration of the surface of Si–Cu(100) free films have been investigated. Specifically, it has been shown that Ba ion implantation and subsequent annealing make it possible to obtain BaSi nanofilms with some excess (to 10 at %) of unbounded silicon atoms.

Simulation of light scattering and electron energy loss (EELS) by small spatially dispersive plasmonic nanodimers

Despite the tremendous progress in the field of nanoplasmonics, complex plasmonic nanostructures such as dimers still present a significant challenge for proper numerical characterization. In particular, for the particles which size is smaller than electron mean free path in metals it is necessary to account for effects of spatial nonlocality in simulations. To address these problems a new mathematical model based on the Discrete Sources Method was developed and corresponding computer model was implemented. It enables one to successfully resolve the problem of accounting for spatial dispersion effects, which is especially important in the systems with very small gap and particle size, and it retains all key features of the Discrete Sources numerical approach including flexibility and a posteriori error estimation. We compute such scattering characteristics as electron energy loss probability and total scattered field intensity of nanodimers as one typically has to rely on methods of both optical and electron spectroscopy to experimentally investigate these structures. The developed model also allows to simultaneously consider several external excitations of the plasmonic system, including both electron and plane wave, in order to obtain more information about the system and to significantly speed up computational performance.