Electrochemical Detection Of Cholesterol Research Articles

Electrochemical detection of cholesterol in human biofluid using microneedle sensor.

The development of a straightforward, economical, portable, and highly sensitive sensing platform for the rapid detection of cholesterol is desirable for the early diagnosis of several pathologic conditions. In this work, we present a fascinating skin-worn microneedle sensor for monitoring cholesterol in interstitial fluid samples. The microneedle sensor was developed by incorporating platinum (Pt) and silver (Ag) wires within pyramidal microneedles containing a microcavity opening; cholesterol oxidase (ChOx) was coupled on the Pt transducer surface using bovine serum albumin and Nafion. Under optimal conditions, the enzymatic microneedle sensor exhibited high sensitivity (0.201 μA μM-1) towards cholesterol in buffer solution, with good linearity over the 1-20 μM range and a correlation coefficient of 0.9910. The analytical performance of the microneedle sensor was also investigated in artificial interstitial fluid and a skin-mimicking phantom gel; the sensor showed great potential for skin-worn/wearable applications with excellent linearity and a low detection limit. In addition, the developed microneedle sensor showed satisfactory stability and good selectivity towards cholesterol in the presence of potential interfering biomolecules, including glucose, lactic acid, uric acid, and ascorbic acid. This sensor exhibits enormous promise for straightforward, sensitive, and minimally invasive monitoring of cholesterol.

Fabrication of novel chitosan nanofiber/gold nanoparticles composite towards improved performance for a cholesterol sensor

A novel amperometric cholesterol biosensor was fabricated by the immobilization of ChOx (cholesterol oxidase) onto the chitosan nanofibers/gold nanoparticles (designated as CSNFs/AuNPs) composite network (NW). The fabrication involves preparation of chitosan nanofibers (CSNFs) and subsequent electrochemical loading of gold nanoparticles (AuNPs). Field emission scanning electron microscopy (FE-SEM) was used to investigate the morphology of CSNFs (sizes in the range of ∼50–100 nm) and spherical AuNPs. Cyclic voltammetry, hydrodynamic voltammetry and amperometry were used to examine the performance of CSNF–AuNPs/ChOx biosensor. The CSNF–AuNPs/ChOx biosensor exhibited a wide linear response to cholesterol (concentration range of 1–45 μM), good sensitivity (1.02 μA/μM), low response time (∼5 s) and excellent long term stability. The combined existence of AuNPs within CSNFs NW provides the excellent performance of the biosensor towards the electrochemical detection of cholesterol.