To realize the design of mobile phone human movement breathing and electrocardiograph (ECG) signal acquisition system based on Bluetooth transmission, the principle of the generation and detection of ECG and respiratory signal and the guide system of signal acquisition are analyzed. Additionally, the hardware of the system is designed, including the hardware of the signal acquisition system, the design of ADS1292R ECG and respiratory signal acquisition module, the design of the main control chip and the design of the Bluetooth module. Then, the digital filtering processing of the ECG and respiratory signals is completed, including the baseline drift filtering and the suppression of the power frequency interference. The results show that the monitoring system runs well and it can effectively collect ECG and respiratory signals, calculate heart rate and respiratory frequency in real time, and display ECG waveform in real time. To sum up, the monitoring system is of great significance for real-time monitoring of the patient's condition.