Mapping Of Resistance Research Articles

The parental inbred lines of the Nested Association Mapping (NAM) population of corn show sources of resistance to tar spot in northern Indiana.

Tar spot is a major foliar disease of corn caused by the obligate fungal pathogen Phyllachora maydis, first identified in Indiana in 2015. Under conducive weather conditions, P. maydis causes significant yield losses in the U.S. and other countries constituting a major threat to corn production. Relatively little is known about resistance to tar spot other than a major quantitative gene that was identified in tropical maize lines. To test for additional sources of resistance against populations of P. maydis in North America, twenty-six parental inbred lines of the Nested Associated Mapping (NAM) population were evaluated for tar spot resistance in Indiana in replicated field trials under natural infection for 3 years. Tar spot disease severity was scored visually using a 0 to 100 % scale. Maximum disease severities (MDS) for tar spot scoring at reproductive growth stage ranged from 0 to 48.3 % with 0% being most resistant and 48.3% being most susceptible. Nine inbred lines were resistant to P. maydis with MDS ranging from 0 to 5.0%, six were moderately resistant (5.2 to 10.6% MDS), two were moderately susceptible (11.7 to 26.0% MDS) and the remaining eight inbred lines were rated as susceptible (30.0 to 48.3% MDS). There was some variability between years, due to higher disease pressure after 2019. Inbred B73, the common parent of the NAM populations, was rated as susceptible with MDS of 30.0%. The nine highly resistant lines provide a potential source of new genes for genetic analysis and mapping of tar spot resistance in corn.

Characterization and Molecular Mapping of a Gene Conferring High-Temperature Adult-Plant Resistance to Stripe Rust Originally from Aegilops ventricosa.

Wheat near-isogenic line AvSYr17NIL carrying Yr17, originally from Aegilops ventricosa for all-stage resistance to Puccinia striiformis f. sp. tritici, also shows nonrace-specific, high-temperature adult-plant (HTAP) resistance to the stripe rust pathogen. To separate and identify the HTAP resistance gene, seeds of AvSYr17NIL were treated with ethyl methanesulfonate. Mutant lines with only HTAP resistance were obtained, and one of the lines, M1225, was crossed with the susceptible recurrent parent Avocet S (AvS). Field responses of the F2 plants and F3 lines, together with the parents, were recorded at the adult-plant stage in Pullman and Mount Vernon, WA under natural P. striiformis f. sp. tritici infection. The parents and the F4 population were phenotyped with a Yr17-virulent P. striiformis f. sp. tritici race in the adult-plant stage under the high-temperature profile in the greenhouse. The phenotypic results were confirmed by testing the F5 population in the field under natural P. striiformis f. sp. tritici infection. The F2 data indicated a single recessive gene, temporarily named YrM1225, for HTAP resistance. The F4 lines were genotyped with Kompetitive allele-specific PCR markers converted from single-nucleotide polymorphism markers polymorphic between M1225 and AvS. The HTAP resistance gene was mapped on the short arm of chromosome 2A in an interval of 7.5 centimorgans using both linkage and quantitative trait locus mapping approaches. The separation of the HTAP resistance gene from Yr17 should improve the understanding and utilization of the different types of resistance.

Mapping of a soybean rust resistance in PI 594756 at the Rpp1 locus.

The online version contains supplementary material available at 10.1007/s11032-023-01358-4.

Smart Artificial Soft Tissue - Application to a Hybrid Simulator for Training of Laryngeal Pacemaker Implantation.

Surgical simulators are safe and evolving educational tools for developing surgical skills. In particular, virtual and hybrid simulators are preferred due to their detailedness, customization and evaluation capabilities. To accelerate the revolution of a novel class of hybrid simulators, a Smart Artificial Soft Tissue is presented here, that determines the relative position of conductive surgical instruments in artificial soft tissue by inverse resistance mappings without the need for a fixed reference point. This is particularly beneficial for highly deformable structures when specific target regions need to be reached or avoided. The carbon-black-silicone composite used can be shaped almost arbitrarily and its elasticity can be tuned by modifying the silicone base material. Thus, objective positional feedback for haptically correct artificial soft tissue can be ensured. This is demonstrated by the development of a laryngeal phantom to simulate the implantation of laryngeal pacemaker electrodes. Apart from the position-detecting larynx phantom, the simulator uses a tablet computer for the virtual representation of the vocal folds' movements, in accordance with the electrical stimulation by the inserted electrodes. The possibility of displaying additional information about target regions and anatomy is intended to optimize the learning progress and illustrates the extensibility of hybrid surgical simulators.

Application of Helicopter-Borne and Ground–Airborne Electromagnetic Detection to Tunnel Engineering Investigation

The Cedaya-S340 Holgutu Highway Construction Project is located in the Mongolian Autonomous Prefecture of Bayingolin in the Xinjiang Uygur Autonomous Region. It is an important traffic channel that connects Luntai County and Hejing County at the southern foot of Tianshan Mountains. As the major component of the highway project, the Huola Mountain Tunnel has a sharp topographic relief, and, therefore, commonly used land geophysical detection instruments cannot work on it. Therefore, we conducted a qualitative survey on the ground-to-air and airborne electromagnetic detection methods used at the Huola Mountain Tunnel site to provide basic data for the design of highway tunnels. The geophysical survey summarized the ground–airborne frequency-domain electromagnetic method (GAFEM) and the helicopter-borne time-domain electromagnetic method (HTEM) developed by Jilin University, and measured 15 measuring lines. Apparent resistivity imaging was performed for each section, and the results were consistent. This study comprehensively analyzed the apparent resistivity profile and geological mapping data. Then, the study inferred the major stratigraphic boundaries, fault fracture zones, rock fragmentation, weakness, karst development, and water content in accordance with background value, low-resistivity anomaly shape, low-resistivity anomaly value, and gradient value in the apparent resistivity profile. Finally, the study identified the scope of two main low-resistivity anomalies, located at the tunnel entrance and exit, respectively, which are basically consistent with the known fault location. The results of this study show that on the basis of the apparent resistivity maps of GAFEM and HTEM, the overall distribution law is basically consistent with site landform, hydrogeology, tectonic geology, and aerial image data. The results provide guidance for the construction of the Huola Mountain Tunnel and ensure the construction safety and progress of the tunnel.

Galaxy ASIST: A web-based platform for mapping and assessment of global standards of antimicrobial susceptibility: A case study in Acinetobacter baumannii genomes.

Antimicrobial susceptibility testing (AST) is used to determine the susceptibility of an organism to antibiotics. The determination of susceptibility is based on MIC breakpoints and is provided by EUCAST and CLSI. Likewise, phenotypic classification criteria developed by CDC/ECDC are used for the classification of pathogens into susceptible, multidrug-resistant, extremely drug-resistant, or totally drug-resistant categories. Whole-genome sequencing (WGS)-based diagnosis is now supplementing existing gold-standard microbiology methods for rapid and more precise AST, and therefore, EUCAST recommended quality criteria to assess whole-genome sequence for reporting the same. In this study, these three global standards, MIC breakpoints, phenotypic classification, and genome quality, are applied to the largest publicly available data for Acinetobacter baumannii (AB), the most critical priority pathogen identified by WHO. The drug sensitivity profile and genomes for isolates of AB were obtained from PATRIC and evaluated with respect to AST standards (CLSI and EUCAST). Whole genome quality assessment and antimicrobial resistance mapping is performed with QUAST and ABRicate, respectively. Four in-house methods are developed for mapping standards and are integrated into a Galaxy workflow based system, Galaxy-ASIST. Analysis of the extent of agreement between CLSI 2022 and EUCAST 2022 for antibiotics was carried out using Cohen's kappa statistics. An automated pipeline, Galaxy-ASIST, is designed and developed for the characterization of clinical isolates based on these standards. Evaluation of over 6,500 AB strains using Galaxy-ASIST indicated that only 10% of the publicly available datasets have metadata to implement these standards. Furthermore, given that CLSI and EUCAST have different MIC breakpoints, discrepancies are observed in the classification of resistant and susceptible isolates following these standards. It is, therefore, imperative that platforms are developed that allow the evaluation of ever increasing phenotypic and genome sequence datasets for AST. Galaxy-ASIST offers a centralized repository and a structured metadata architecture to provide a single globally acceptable framework for AST profiling of clinical isolates based on global standards. The platform also offers subsequent fine mapping of antimicrobial-resistant determinants. Galaxy-ASIST is freely available at https://ab-openlab.csir.res.in/asist.

Inheritance of Early and Late Ascochyta Blight Resistance in Wide Crosses of Chickpea.

Chickpea (Cicer arietinum) is a globally important food legume but its yield is negatively impacted by the fungal pathogen Ascochyta blight (Ascochyta rabiei) causing necrotic lesions leading to plant death. Past studies have found that Ascochyta resistance is polygenic. It is important to find new resistance genes from the wider genepool of chickpeas. This study reports the inheritance of Ascochyta blight resistance of two wide crosses between the cultivar Gokce and wild chickpea accessions of C. reticulatum and C. echinospermum under field conditions in Southern Turkey. Following inoculation, infection damage was scored weekly for six weeks. The families were genotyped for 60 SNPs mapped to the reference genome for quantitative locus (QTL) mapping of resistance. Family lines showed broad resistance score distributions. A late responding QTL on chromosome 7 was identified in the C. reticulatum family and three early responding QTLs on chromosomes 2, 3, and 6 in the C. echinospermum family. Wild alleles mostly showed reduced disease severity, while heterozygous genotypes were most diseased. Interrogation of 200k bp genomic regions of the reference CDC Frontier genome surrounding QTLs identified nine gene candidates involved in disease resistance and cell wall remodeling. This study identifies new candidate chickpea Ascochyta blight resistance QTLs of breeding potential.

Analysis of Genetic Diversity, Population Structure and Association Mapping for Late Blight Resistance in Potato (Solanum tuberosum L.) Accessions Using SSR Markers

The allelic variations in a diversity panel of 353 potato accessions, including 256 accessions belonging to Solanum tuberosum sub spp. tuberosum, 49 accessions belonging to Solanum tuberosum sub spp. andigena, and 48 Indian potato varieties were analysed using 25 simple sequence repeat (SSR) markers. The SSR allelic profiles revealed high levels of polymorphism and distinctness among the accessions studied. A total of 343 alleles of 25 SSR markers were observed in the diversity panel of 353 highly diverse tetraploid potato accessions. The number of alleles produced per SSR varied from 8 for the marker STM1053 to 25 for the marker STIKA. The polymorphic information content (PIC) ranged from 0.66 (STG0010) to 0.93 (STM1106) with an average of 0.82. The cluster analysis using the SSR allelic profiles of 353 accessions divided the population into five major groups. The association mapping for late blight resistance identified six markers with the general linear model (GLM), and out of these six markers significance of three markers was reconfirmed with the mixed linear model (MLM). The findings of this study suggest that SSRs are the appropriate markers for evaluating genetic diversity and population structure within different potato germplasm collections. A significant diversity across the tetraploid potato accessions was observed. Moreover, the markers identified in this study could be useful in marker-assisted selection (MAS) breeding in potato for late blight resistance (LBR).

Intrinsic resistance of HIV-2 and SIV to the maturation inhibitor GSK2838232.

GSK2838232 (GSK232) is a novel maturation inhibitor that blocks the proteolytic cleavage of HIV-1 Gag at the junction of capsid and spacer peptide 1 (CA/SP1), rendering newly-formed virions non-infectious. To our knowledge, GSK232 has not been tested against HIV-2, and there are limited data regarding the susceptibility of HIV-2 to other HIV-1 maturation inhibitors. To assess the potential utility of GSK232 as an option for HIV-2 treatment, we determined the activity of the compound against a panel of HIV-1, HIV-2, and SIV isolates in culture. GSK232 was highly active against HIV-1 isolates from group M subtypes A, B, C, D, F, and group O, with IC50 values ranging from 0.25-0.92 nM in spreading (multi-cycle) assays and 1.5-2.8 nM in a single cycle of infection. In contrast, HIV-2 isolates from groups A, B, and CRF01_AB, and SIV isolates SIVmac239, SIVmac251, and SIVagm.sab-2, were highly resistant to GSK232. To determine the role of CA/SP1 in the observed phenotypes, we constructed a mutant of HIV-2ROD9 in which the sequence of CA/SP1 was modified to match the corresponding sequence found in HIV-1. The resulting variant was fully susceptible to GSK232 in the single-cycle assay (IC50 = 1.8 nM). Collectively, our data indicate that the HIV-2 and SIV isolates tested in our study are intrinsically resistant to GSK232, and that the determinants of resistance map to CA/SP1. The molecular mechanism(s) responsible for the differential susceptibility of HIV-1 and HIV-2/SIV to GSK232 require further investigation.

Inferring population connectivity in eastern massasauga rattlesnakes (Sistrurus catenatus) using landscape genetics.

Assessing the environmental factors that influence the ability of a threatened species to move through a landscape can be used to identify conservation actions that connect isolated populations. However, direct observations of species' movement are often limited, making the development of alternate approaches necessary. Here we use landscape genetic analyses to assess the impact of landscape features on the movement of individuals between local populations of a threatened snake, the eastern massasauga rattlesnake (Sistrurus catenatus). We linked connectivity data with habitat information from two landscapes of similar size: a large region of unfragmented habitat and a previously studied fragmented landscape consisting of isolated patches of habitat. We used this analysis to identify features of the landscape where modification or acquisition would enhance population connectivity in the fragmented region. We found evidence that current connectivity was impacted by both contemporary land-cover features, especially roads, and inherent landscape features such as elevation. Next, we derived estimates of expected movement ability using a recently developed pedigree-based approach and least-cost paths through the unfragmented landscape. We then used our pedigree and resistance map to estimate resistance polygons of the potential extent for S. catenatus movement in the fragmented landscape. These polygons identify possible sites for future corridors connecting currently isolated populations in this landscape by linking the impact of future habitat modification or land acquisition to dispersal ability in this species. Overall, our study shows how modeling landscape resistance across differently fragmented landscapes can identify habitat features that affect contemporary movement in threatened species in fragmented landscapes and how this information can be used to guide mitigation actions whose goal is to connect isolated populations.

Association mapping for broomrape resistance in sunflower.

Sunflower breeding for resistance to the parasitic plant sunflower broomrape (Orobanche cumana Wallr.) requires the identification of novel resistance genes. In this research, we conducted a genome-wide association study (GWAS) to identify QTLs associated with broomrape resistance. The marker-trait associations were examined across a germplasm set composed of 104 sunflower accessions. They were genotyped with a 600k AXIOM® genome-wide array and evaluated for resistance to three populations of the parasite with varying levels of virulence (races EFR, FGV, and GTK) in two environments. The analysis of the genetic structure of the germplasm set revealed the presence of two main groups. The application of optimized treatments based on the general linear model (GLM) and the mixed linear model (MLM) allowed the detection of 14 SNP markers significantly associated with broomrape resistance. The highest number of marker-trait associations were identified on chromosome 3, clustered in two different genomic regions of this chromosome. Other associations were identified on chromosomes 5, 10, 13, and 16. Candidate genes for the main genomic regions associated with broomrape resistance were studied and discussed. Particularly, two significant SNPs on chromosome 3 associated with races EFR and FGV were found at two tightly linked SWEET sugar transporter genes. The results of this study have confirmed the role of some QTL on resistance to sunflower broomrape and have revealed new ones that may play an important role in the development of durable resistance to this parasitic weed in sunflower.

Unraveling the Geothermal Potential of Corbetti Caldera Using Integrated Geophysical Methods, Central Main Ethiopian Rift

In this paper integrated geophysical techniques, involving electrical resistivity, gravity and magnetic surveys have been carried out over the Corbetti Caldera in the central Main Ethiopian Rift (CMER) to verify the geothermal potential of the caldera. Corbetti geothermal prospect is located at about 250 km south of Addis Ababa and 20km from Awassa city and the area is bounded by Lake Awassa to the south and Lake Shalla to the north with geographic location between latitude 7.170N-7.250N and longitudes 38.300E-38.470E. The Corbetti Caldera is characterized by Quaternary volcano-tectonic activity which is mainly silicic volcanism and a resurgent caldera structural system. Quaternary volcanism is associated with a wide spread of steaming ground and fumarolic activity which could confirm the existence of a heat source at depth. Fifty six (56) VES points, 200 gravity and about 200 magnetic data have been used and analyzed. The results are presented as magnetic, gravity and electrical counter maps for qualitative interpretation. Constrained gravity and magnetic 2D model and results obtained from the geoelectric section were made for quantitative interpretation. The complete Bouguer gravity anomaly in conjugation with the total magnetic field anomaly map of the area indicates the existence of intrusion beneath the caldera, i.e. the highest Bouguer gravity anomaly resulting from the higher density of the intrusion. Correspondingly the shallower heat source caused by this intrusion is characterized by the lowest magnetic anomaly response. Results from vertical electrical soundings along profile-2 indicate the presence of a middle thicker conductive zone which is associated with the increase in temperature and alteration of rocks. Apparent resistivity map for AB/2=1810m and AB/2=2700m shows low resistivity anomaly follows the eastern and northern caldera rim, stretching north of the caldera towards Lake Shalla.

QTL mapping and candidate gene sequencing for disease resistance in rice - Review

QTL mapping and candidate gene sequencing for disease resistance in rice - Review

Mapping and characterization of a novel adult-plant leaf rust resistance gene LrYang16G216 via bulked segregant analysis and conventional linkage method.

A novel adult-plant leaf rust resistance gene LrYang16G216 on wheat chromosome 6BL was identified and mapped to a 0.59cM genetic interval by BSA and conventional linkage method. Leaf rust (Puccinia triticina) is one of the most devastating fungal diseases of wheat (Triticum aestivum L.). Discovery and identification of new resistance genes is essential to develop disease-resistant cultivars. An advanced breeding line Yang16G216 was previously identified to confer adult-plant resistance (APR) to leaf rust. In this research, a recombinant inbred line (RIL) population was constructed from the cross between Yang16G216 and a highly susceptible line Yang16M6393, and genotyped with exome capture sequencing and 55K SNP array. Through bulked segregant analysis (BSA) and genetic linkage mapping, a stable APR gene, designated as LrYang16G216, was detected and mapped to the distal region of chromosome arm 6BL with a genetic interval of 2.8cM. For further verification, another RIL population derived from the cross between Yang16G216 and a susceptible wheat variety Yangmai 29 was analyzed using the enriched markers in the target interval, and LrYang16G216 was further narrowed to a 0.59cM genetic interval flanked by the KASP markers Ax109403980 and Ax95083494, corresponding to the physical position 712.34-713.94Mb in the Chinese Spring reference genome, in which twenty-six disease resistance-related genes were annotated. Based on leaf rust resistance spectrum, mapping data and physical location, LrYang16G216 was identified to be a novel and effective APR gene. The LrYang16G216 with linked markers will be useful for marker-assisted selection in wheat resistance breeding.

Mapping and validation of all-stage resistance to stem rust in four South African winter wheat cultivars

Mapping and validation of all-stage resistance to stem rust in four South African winter wheat cultivars

Biology of Vectors and Agent of Skin Disease and River Blindness (Onchocerciasis) In the World

Objectives: Blackflies are the aquatic insects. A total of 1800 species of this insect have been identified worldwide. The main important species is Simulium damnosum. They suck the blood of other animals and human. They spread several diseases, including river blindness (Onchocerciasis)) in Africa and America. The agent of disease is Onchocerca volvulus. The aim of this review article is to explain the bio ecology, medically importance, different control measures for agent and vector of disease and status of resistant to drug and insecticides in the agent and vector. Methods: In this research all, the relevant information regarding the topic of research is research through the internet and used in this paper. An intensive search of scientific literature was done in “PubMed”, “Web of Knowledge”, “Scopus”, “Google Scholar”, “SID”, etc. Results: The results indicated that this insect play an important role in disease worldwide. Ivermectine (Mectizan®) drug is an important for killing the agent of disease. Several larvicides recommended by WHO for vector control. Due to high use of drug and pesticide for control of disease , the resistant to drug and pesticide have been reported worldwide. Conclusion: Monitoring and mapping of drug and insecticide resistance is a major factor for disease control worldwide

Identification of spatial conservation and restoration priorities for ecological networks planning in a highly urbanized region: A case study in Beijing-Tianjin-Hebei, China

Building ecological networks is not only critical for biodiversity conservation, but also for sustainable urban development. It is of great significance to integrate a connected network of protected areas into spatial planning as human-dominated land use changes are mostly a non-reversible process. Taking the Beijing-Tianjin-Hebei (BTH) region as an example, the Least Cost Path (LCP) model is adopted for ecological networks simulation at regional scale based on a resistance map composed by different factors (i.e., naturalness of land covers, the riparian corridor, and disturbance of roads) derived from very high resolution spatial data. Then, all least cost paths are classified as green or blue paths, and evaluated according to their contribution to the whole network connectivity. Two types of prioritized locations are identified for restoration and conservation of the ecological networks: breaking points and stepping stones. The results show that there are a total of 246 potential ecological corridors between the protected areas, and 6 breaking points that need to be restored and 12 intersection points of LCPs (particularly with the blue paths) that are considered as stepping stones enhancing networks connectivity. Further spatial overlay of the ecological networks and Major Function Oriented Zone (MFOZ) plan shows that the current regional development strategy needs to be adapted to protect the integrity of the ecological networks from possible disturbance of human activities. On-site verification has proved that our results can provide reliable instructions for spatial conservation and restoration of ecological networks and contribute to the optimization of spatial planning in practice.

Determination of aquifer position, aquifer protective capacity and groundwater quality in selected dumpsite in obubra local government area of south eastern Nigeria

At Obubra Urban in Cross River State, aquifer position, aquifer protective capacity, and groundwater quality assessment were carried out. With a maximum electrode spacing of 480 meters, 12 vertical electrical sounding (VES) surveys were conducted. The data collected were processed using the IPI2win program. employing five locations for a 2D survey A resistivity map of the dumpsites was also produced using electrical resistivity tomography (ERT). While traditional laboratory techniques were utilized to examine the groundwater, tritium tagging investigations were also conducted to determine the extent of infiltration in the study area. Four to five geoelectric sections are shown in the study. The aquifer is located at depths of 51.7, 56.4, 34.5, and 56.4 meters in the third, fourth, and fifth layers, respectively. While other VES locations have values between 0.06g and 015 mhos and are classed as weakly protected, the over burden protection capacity has values of 0.3, 0.2, 0.18, and 0.17 mhos at VES 1, 4, 7, and 8, respectively. The area's aquifer system is likely extremely permeable with strong storativity because of the area's high porosity and transmissivity characteristics. The area has an infiltration value between 7 and 21 percent, according to the tritium tagging technique used to measure the extent of infiltration from the dumpsite. The aquifer system in the research area is porous, according to the results of the procedures described above, and toxins will circulate through it quickly if they are contaminated. The investigation reveals that while magnesium and cadmium were present in quantities beyond the WHO-approved limits for portable water, all other psychochemical markers were within acceptable limits.

Prediction of Soil Bearing Capacity in the Masterplan Area of the Kalimantan Institute of Technology based on GIS

Prediction of bearing capacity and soil type is a requirement for the safety of construction before planning a building construction. Therefore, it is necessary to research the distribution of bearing capacity and conical resistance to determine soil conditions in an area. Based on CPT data, this study mapped cone resistance distribution and soil-bearing capacity distribution in the ITK masterplan area. The analysis was carried out by reviewing the 40 cm pile foundation at 11 m. The analysis was carried out using the Kriging, IDW, and Spline with Barriers methods. The bearing capacity was analyzed using the Trofimankove method. The mapping of the carrying capacity using the three interpolation methods results in a Qall value of around 26,024 – 87,835 tons. The cone resistance mapping results using the three interpolation methods show that the soil consistent in the ITK masterplan area is stiff, very stiff and Hard with a qc value of around 16,0804 – 259,54 kg/cm2. The ITK masterplan area has a type of foundation soil, which is hard. The comparison results obtained from mapping the cone resistance and the carrying capacity of the three interpolation methods used, the value of the range of qc and Qall closest to the sample data used is the IDW method

Geophysical data provide three dimensional insights into porphyry copper systems in the Silverton caldera, Colorado, USA

The Silverton caldera in southwest Colorado, USA hosts polymetallic veins and pervasively altered rocks indicative of porphyry copper systems. Nearly a kilometer of erosion has exposed multiple levels of the hydrothermal systems from shallow lithocaps down to quartz-sericite-pyrite veins. New airborne electromagnetic and magnetic survey data are integrated with previous alteration mapping and porphyry models to show the subsurface geophysical response of shallow to deep levels of the porphyry system. Qualitative map views show lateral changes in the magnetization and resistivity of the hydrothermally altered rocks. The volcanic terrain exhibits high magnetization and high amplitude anomalies map near-surface plutonic rocks associated with porphyry systems. Magnetic susceptibility measurements on outcrops of hydrothermally altered rocks indicate magnetite content decreases upward and outward from the source intrusions where magnetic anomaly lows are observed over the lithocaps. The resistivity maps highlight hydrothermal alteration as resistivity lows with exception being rocks having propylitic alteration. Quantitative resistivity models show low resistivity zones with an apparent thickness around 50–150 m beneath quartz-sericite-pyrite veins interpreted to be the result of supergene processes that may continue today, and the calculated magnetic source depths occur near the top of this zone. The resistivity models also show rocks having propylitic, silicic, and quartz-alunite-pyrophyllite assemblages exhibit high resistivity with depth, and argillic alteration assemblages had high resistivity due to high quartz content. This integrated approach presented in a three-dimensional environment provides guidance when exploring for porphyry copper systems in less exposed terrains.