Membrane Electrical Properties Research Articles

Sulfonated graphene oxide linked with alkali metal ions membranes for proton conductivity in hydrogen production from water electrolysis

Amid technological advances, rising energy demand, and environmental concerns, countries endorse green energy solutions. Prominent among them is hydrogen production via clean water electrolysis, a key strategy for sustainable development. Herein, to address the limitations of expensive and potentially harmful Nafion membranes, we have developed a new graphene oxide (GO) series membranes (hydrophilic sulfonated GO (GO-SO3H) linked with monovalent alkali metal ions were synthesized to fabricate the membranes) to be used in water electrolysis for hydrogen production by introducing the iodide oxidation reaction (IOR) as an alternative to the traditional oxygen evolution reaction (OER). Various modifications by sulfuric acid, methanol, and different alkali metal hydroxides (LiOH, NaOH, KOH) result in membranes with distinct properties. Characterization techniques, including ATR-FTIR, SEM, AFM, XRD, water contact angle (WCA), and electrochemical impedance spectroscopy (EIS), validated the modifications, assessed membrane morphology, interlayer spacing, hydrophilicity, and electrical properties. Among the membranes, GO-SO3K stood out, exhibiting the highest proton conductivity (119.16 mS) and superior potassium ion permeability (20.382 cm/s). Importantly, all GO series membranes demonstrate excellent stability in the electrochemical system. The findings suggested that GO-SO3K holds promise as a potassium ion-conductive membrane for efficient and sustainable hydrogen production, offering a viable alternative to conventional Nafion membranes in water electrolysis applications.

Droplet Polymer Bilayers for Bioelectronic Membrane Interfacing.

Model membranes interfaced with bioelectronics allow for the exploration of fundamental cell processes and the design of biomimetic sensors. Organic conducting polymers are an attractive surface on which to study the electrical properties of membranes because of their low impedance, high biocompatibility, and hygroscopic nature. However, establishing supported lipid bilayers (SLBs) on conducting polymers has lagged significantly behind other substrate materials, namely, for challenges in membrane electrical sealing and stability. Unlike SLBs that are highly dependent on surface interactions, droplet interface bilayers (DIBs) and droplet hydrogel bilayers (DHBs) leverage the energetically favorable organization of phospholipids at atomically smooth liquid interfaces to build high-integrity membranes. For the first time, we report the formation of droplet polymer bilayers (DPBs) between a lipid-coated aqueous droplet and the high-performing conducting polymer poly(3,4-ethylenedioxythiophene) polystyrenesulfonate (PEDOT:PSS). The resulting bilayers can be produced from a range of lipid compositions and demonstrate strong electrical sealing that outcompetes SLBs. DPBs are subsequently translated to patterned and planar microelectrode arrays to ease barriers to implementation and improve the reliability of membrane formation. This platform enables more reproducible and robust membranes on conducting polymers to further the mission of merging bioelectronics and synthetic, natural, or hybrid bilayer membranes.

Sulfonated poly(ether ether ketone)-Protic ionic Liquid-Based anhydrous Proton-Conducting composite polymer electrolyte membranes for High-Temperature fuel cell applications

A high-temperature polymer electrolyte membrane fuel cell (PEMFC) has enormous potential to produce clean energy with minimal environmental pollution along with high energy density and conversion efficiency. In the present work, anhydrous proton-conducting polymer electrolyte membranes for prospective high-temperature fuel cell application were successfully prepared using different protic ionic liquids (PILs) and sulfonated poly(ether ether ketone) (SPEEK). The protic ionic liquids (PILs), 2-hydroxyethylammonium formate, diethylmethyl ammonium triflate, 1-ethylimidazolium bis(trifluoromethylsulfonyl)imide, 1,2-dimethyl imidazolium bis(trifluoro methylsulfonyl)imide, and diethylmethylammonium methanesulfonate were selected based on their favorable physicochemical properties. The effect of the incorporation of the PIL on the structural, thermal, and electrical transport properties of the composite SPEEK polymer electrolyte membranes was studied. The prepared polymer electrolyte membranes (PEMs) were characterized using Fourier transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), X-ray diffractometry (XRD), and electrochemical impedance spectroscopy (EIS) techniques. FTIR results indicated an interaction between the PIL and the SPEEK, which resulted in homogeneous and flexible membranes. The DSC results confirmed good miscibility and elucidated plasticizing effect of the incorporated PIL on the SPEEK polymer matrix. All the PEMs showed good thermal stability and high-temperature anhydrous proton conductivity, achieving best proton conductivity, ≈8 × 10–3 S cm−1 at 120 °C and low activation energy ≈0.26 eV, making it suitable for prospective high-temperature PEMFC applications.

Hijacking of internal calcium dynamics by intracellularly residing viral rhodopsins.

Rhodopsins are ubiquitous light-driven membrane proteins with diverse functions, including ion transport. Widely distributed, they are also coded in the genomes of giant viruses infecting phytoplankton where their function is not settled. Here, we examine the properties of OLPVR1 (Organic Lake Phycodnavirus Rhodopsin) and two other type 1 viral channelrhodopsins (VCR1s), and demonstrate that VCR1s accumulate exclusively intracellularly, and, upon illumination, induce calcium release from intracellular IP3-dependent stores. In vivo, this light-induced calcium release is sufficient to remote control muscle contraction in VCR1-expressing tadpoles. VCR1s natively confer light-induced Ca2+ release, suggesting a distinct mechanism for reshaping the response to light of virus-infected algae. The ability of VCR1s to photorelease calcium without altering plasma membrane electrical properties marks them as potential precursors for optogenetics tools, with potential applications in basic research and medicine.

Effect of gamma irradiation on the electrical and optical properties of PEVA composite membrane embedded with conductive copper fluoroborate glass powder

Effect of gamma irradiation on optical bandgap of PEVA/glass-powder membranes.

Effects of systemic oxytocin administration on ultraviolet B-induced nociceptive hypersensitivity and tactile hyposensitivity in mice.

Ultraviolet B (UVB) radiation induces cutaneous inflammation, leading to thermal and mechanical hypersensitivity. Here, we examine the mechanical properties and profile of tactile and nociceptive peripheral afferents functionally disrupted by this injury and the role of oxytocin (OXT) as a modulator of this disruption. We recorded intracellularly from L4 afferents innervating the irradiated area (5.1J/cm2) in 4-6 old week male mice (C57BL/6J) after administering OXT intraperitoneally, 6mg/Kg. The distribution of recorded neurons was shifted by UVB radiation to a pattern observed after acute and chronic injuries and reduced mechanical thresholds of A and C- high threshold mechanoreceptors while reducing tactile sensitivity. UVB radiation did not change somatic membrane electrical properties or fiber conduction velocity. OXT systemic administration rapidly reversed these peripheral changes toward normal in both low and high-threshold mechanoreceptors and shifted recorded neuron distribution toward normal. OXT and V1aR receptors were present on the terminals of myelinated and unmyelinated afferents innervating the skin. We conclude that UVB radiation, similar to local tissue surgical injury, cancer metastasis, and peripheral nerve injury, alters the distribution of low and high threshold mechanoreceptors afferents and sensitizes nociceptors while desensitizing tactile units. Acute systemic OXT administration partially returns all of those effects to normal.

Novel chitosan-ionic liquid immobilized membranes for PEM fuel cells operating above the boiling point of water

Novel chitosan (CS) composite membranes modified with ionic liquids (ILs), namely, 1-Hexyl-3- methylimidazolium tricyanomethanide ([HMIM][TCM]), Diethylmethylammonium methanesulfonate ([DEMA][OMs]), were synthesized to be used as a polymer electrolyte membrane (PEM) in for PEM fuel cells. The ionic conductivities of the prepared membrane samples were evaluated using electrochemical impedance spectroscopy (EIS). The CS is one of the attractive choices of green and sustainable membrane materials in PEMs because of its chemical tunability and cost-effectiveness. The membranes were prepared by solution casting followed by a solvent evaporating procedure. The low proton conductivity of pristine CS biopolymer was enhanced by the incorporation of ILs in the membrane framework. Tensile testing confirmed the excellent membrane mechanical integrity. A significant enhancement in the membranes’ characteristics was observed upon the introduction of [DEMA][OMs] into its matrix. The experimental results showed enhanced proton conductivities of the prepared membranes along with enhanced flexibility. The proton conductivity of pristine CS membrane was increased from 8.4 × 10−4 to 1.25 × 10−2 S/cm and 1.1 × 10−2 S/cm when a 30 wt% [HMIM][TCM] and 100 wt% [DEMA][OMs] were added, respectively. Synergistic effects of amine and imidazolium-based ILs on the electrical, structural, water uptake, and thermal properties of CS-based biopolymer solid electrolyte membranes are reported for the first time in this work. The aforementioned characterization results demonstrate the potential of using these membranes in PEM fuel cells operating above the boiling point of water and at a lower cost. This work showed that there should be an optimization amount of the ILs content in the membrane to maintain its mechanical integrity.

Conductive and self-cleaning composite membranes from corn husk nanofiber embedded with inorganic fillers (TiO2, CaO, and eggshell) by sol–gel and casting processes for smart membrane applications

Abstract The utilization of corn husk as a renewable bio-cellulose material for producing bio-composite membranes through wet chemical and sol–gel process offers numerous advantages. It is an abundant, inexpensive, nontoxic, and readily available agricultural waste product. To enhance the properties of bio-composite membranes, various particulate ionic fillers such as titanium dioxide, calcium oxide, and eggshell (as a source of calcium carbonate) are incorporated in different weight percentages (0, 1, and 5%). These fillers act as additives to the corn husk nanofiber mixed with polyvinyl alcohol during the formation of the biomembrane. The resulting biocomposite membranes exhibit several desirable characteristics. They are lightweight, easy to shape, biodegradable, nontoxic, and possess excellent physical, mechanical, thermal, and electrical properties. Moreover, the addition of 5 wt% of eggshell powder leads to an increase in the dielectric constant and electrical conductivity, reaching approximately 3.300 ± 0.508 and 1.986 × 103 (Ω·m)−1, respectively. These measurements were taken at a frequency of 500 Hz and a temperature of 27°C. Furthermore, these membranes demonstrate self-cleaning abilities due to a contact angle greater than 90°. The electrical properties of the biocomposite membrane improve with a higher percentage of inorganic filler, making them suitable for applications in smart membranes, as well as mechanical, electrical, and thermal systems.

Cellular excitability and ns-pulsed electric fields: Potential involvement of lipid oxidation in the action potential activation

Recent studies showed that nanosecond pulsed electric fields (nsPEFs) can activate voltage-gated ion channels (VGICs) and trigger action potentials (APs) in excitable cells. Under physiological conditions, VGICs’ activation takes place on time scales of the order 10–100 µs. These time scales are considerably longer than the applied pulse duration, thus activation of VGICs by nsPEFs remains puzzling and there is no clear consensus on the mechanisms involved. Here we propose that changes in local electrical properties of the cell membrane due to lipid oxidation might be implicated in AP activation. We first use MD simulations of model lipid bilayers with increasing concentration of primary and secondary lipid oxidation products and demonstrate that oxidation not only increases the bilayer conductance, but also the bilayer capacitance. Equipped with MD-based characterization of electrical properties of oxidized bilayers, we then resort to AP modelling at the cell level with Hodgkin-Huxley-type models. We confirm that a local change in membrane properties, particularly the increase in membrane conductance, due to formation of oxidized membrane lesions can be high enough to trigger an AP, even when no external stimulus is applied. However, excessive accumulation of oxidized lesions (or other conductive defects) can lead to altered cell excitability.

Changes in Ion Transport across Biological Membranes Exposed to Particulate Matter.

The cells of living organisms are surrounded by the biological membranes that form a barrier between the internal and external environment of the cells. Cell membranes serve as barriers and gatekeepers. They protect cells against the entry of undesirable substances and are the first line of interaction with foreign particles. Therefore, it is very important to understand how substances such as particulate matter (PM) interact with cell membranes. To investigate the effect of PM on the electrical properties of biological membranes, a series of experiments using a black lipid membrane (BLM) technique were performed. L-α-Phosphatidylcholine from soybean (azolectin) was used to create lipid bilayers. PM samples of different diameters (<4 (SRM-PM4.0) and <10 μm (SRM-PM10) were purchased from The National Institute of Standards and Technology (USA) to ensure the repeatability of the measurements. Lipid membranes with incorporated gramicidin A (5 pg/mL) ion channels were used to investigate the effect of PM on ion transport. The ionic current passing through the azolectin membranes was measured in ionic gradients (50/150 mM KCl on cis/trans side). In parallel, the electric membrane capacitance measurements, analysis of the conductance and reversal potential were performed. Our results have shown that PM at concentration range from 10 to 150 μg/mL reduced the basal ionic current at negative potentials while increased it at positive ones, indicating the interaction between lipids forming the membrane and PM. Additionally, PM decreased the gramicidin A channel activity. At the same time, the amplitude of channel openings as well as single channel conductance and reversal potential remained unchanged. Lastly, particulate matter at a concentration of 150 μg/mL did not affect the electric membrane capacity to any significant extent. Understanding the interaction between PM and biological membranes could aid in the search for effective cytoprotective strategies. Perhaps, by the use of an artificial system, we will learn to support the consequences of PM-induced damage.

Nucleoporin Nup358 Downregulation Tunes the Neuronal Excitability in Mouse Cortical Neurons.

Nucleoporins (NUPs) are proteins that comprise the nuclear pore complexes (NPCs). The NPC spans the nuclear envelope of a cell and provides a channel through which RNA and proteins move between the nucleus and the cytoplasm and vice versa. NUP and NPC disruptions have a great impact on the pathophysiology of neurodegenerative diseases (NDDs). Although the downregulation of Nup358 leads to a reduction in the scaffold protein ankyrin-G at the axon initial segment (AIS) of mature neurons, the function of Nup358 in the cytoplasm of neurons remains elusive. To investigate whether Nup358 plays any role in neuronal activity, we downregulated Nup358 in non-pathological mouse cortical neurons and measured their active and passive bioelectrical properties. We identified that Nup358 downregulation is able to produce significant modifications of cell-membrane excitability via voltage-gated sodium channel kinetics. Our findings suggest that Nup358 contributes to neuronal excitability through a functional stabilization of the electrical properties of the neuronal membrane. Hypotheses will be discussed regarding the alteration of this active regulation as putatively occurring in the pathophysiology of NDDs.

Rapid Screening of Urinary Tract Infection Using Microfluidic Inertial-Impedance Cytometry.

Urinary tract infection (UTI) diagnosis based on urine culture for bacteriuria analysis is time-consuming and often leads to wastage of hospital resources due to false-positive UTI cases. Direct cellular phenotyping (e.g., RBCs, neutrophils, epithelial cells) of urine samples remains a technical challenge as low cell concentrations, and urine characteristics (conductivities, pH, microbes) can affect the accuracy of cell measurements. In this work, we report a microfluidic inertial-impedance cytometry technique for label-free rapid (<5 min) neutrophil sorting and impedance profiling from urine directly. Based on size-based inertial focusing effects, neutrophils are isolated, concentrated, and resuspended in saline (buffer exchange) to improve consistency in impedance-based single-cell analysis. We first observed that both urine pH and the presence of bacteria can affect neutrophil high-frequency impedance measurements possibly due to changes in nucleus morphology as neutrophils undergo NETosis and phagocytosis, respectively. As a proof-of-concept for clinical testing, we report for the first time, rapid UTI testing based on multiparametric impedance profiling of putative neutrophils (electrical size, membrane properties, and distribution) in urine samples from non-UTI (n = 20) and UTI patients (n = 20). A significant increase in cell count was observed in UTI samples, and biophysical parameters were used to develop a UTI classifier with an area under the receiver operating characteristic curve of 0.84. Overall, the developed platform facilitates rapid culture-free urine screening which can be further developed to assess disease severity in UTI and other urologic diseases based on neutrophil electrical signatures.

Cx43 Hemichannel and Panx1 Channel Modulation by Gap19 and 10Panx1 Peptides.

Cx43 hemichannels (HCs) and Panx1 channels are two genetically distant protein families. Despite the lack of sequence homology, Cx43 and Panx1 channels have been the subject of debate due to their overlapping expression and the fact that both channels present similarities in terms of their membrane topology and electrical properties. Using the mimetic peptides Gap19 and 10Panx1, this study aimed to investigate the cross-effects of these peptides on Cx43 HCs and Panx1 channels. The single-channel current activity from stably expressing HeLa-Cx43 and C6-Panx1 cells was recorded using patch-clamp experiments in whole-cell voltage-clamp mode, demonstrating 214 pS and 68 pS average unitary conductances for the respective channels. Gap19 was applied intracellularly while 10Panx1 was applied extracellularly at different concentrations (100, 200 and 500 μM) and the average nominal open probability (NPo) was determined for each testing condition. A concentration of 100 µM Gap19 more than halved the NPo of Cx43 HCs, while 200 µM 10Panx1 was necessary to obtain a half-maximal NPo reduction in the Panx1 channels. Gap19 started to significantly inhibit the Panx1 channels at 500 µM, reducing the NPo by 26% while reducing the NPo of the Cx43 HCs by 84%. In contrast 10Panx1 significantly reduced the NPo of the Cx43 HCs by 37% at 100 µM and by 83% at 200 µM, a concentration that caused the half-maximal inhibition of the Panx1 channels. These results demonstrate that 10Panx1 inhibits Cx43 HCs over the 100-500 µM concentration range while 500 µM intracellular Gap19 is necessary to observe some inhibition of Panx1 channels.

Carboxymethyl cellulose membranes blended with carbon nanotubes/Ag nanoparticles for eco-friendly safer lithium-ion batteries

This paper aimed to construct carboxymethyl cellulose (CMC) nanocomposite films incorporated with carbon nanotubes (CNTs) and silver nanoparticles (Ag NPs) by an eco-friendly approach and compared their alternative properties with the pure CMC film. The novelty in this article is the investigation of the dielectric properties of CMC/CNT/Ag, the enhancement of the CMC solubility by citric acid, and the preparation of Ag NPs in a domestic microwave. Treatment of the CMC/CNT/Ag films with citric acid decreased the water solubility and water adsorption by 13.7–83 % and 6–14.3 %, respectively, while enhancing the tensile strength (TS) and elongation at break (EB) by 4–5 % and 81–115 %, respectively, compared to the pristine CMC film. In addition, the pore size and surface area of the prepared films increased due to the incorporation of CNTs. Adding 10 and 20 wt% CNTs and Ag NPs to the treated films decreased the water solubility and adsorption, increasing tensile properties and electrical conductivity. The addition of different concentrations of CNT/Ag to the membrane led to variations in the membrane's electrical properties. CNTs and Ag NPs might be alternatives to nano-fillers for improving the CMC film properties. These CNT/Ag and CMC films could be used as alternative materials in eco-friendly safer lithium-ion batteries.

Saline Retention and Permeability of Nanofiltration Membranes Versus Resistance and Capacitance as Obtained from Impedance Spectroscopy under a Concentration Gradient.

Impedance spectroscopy has been widely used for the study of the electrical properties of membranes for their characterization. The most common use of this technique is the measure of the conductivity of different electrolyte solutions to study the behavior and movement of electrically charged particles inside the pores of membranes. The objective of this investigation was to observe if there is a relation present between the retention that a nanofiltration membrane possesses to certain electrolytic solutions (NaCl, KCl, MgCl2, CaCl2, and Na2SO4) and the parameters that are obtained through IS measurements of the active layer of the membrane. To achieve our objective, different characterization techniques were performed to obtain the permeability, retention, and zeta potential values of a Desal-HL nanofiltration membrane. Impedance spectroscopy measurements were performed when a gradient concentration was present between both sides of the membrane to study the variation that the electrical parameters had with the time evolution.

Effects of storage conditions on permeability and electrical impedance properties of the skin barrier

The aim of this study was to investigate the effect of various skin preservation protocols on in vitro drug permeation, epidermal-dermal drug distribution, and electrical impedance properties of skin membranes. Acyclovir (AC) and methyl salicylate (MS) were selected as model drugs due to their different physicochemical properties and skin metabolic profiles. In particular, AC is relatively hydrophilic (logP −1.8) and not expected to be affected by skin metabolism, while MS is relatively lipophilic (logP 2.5) and susceptible to metabolism, being a substrate for esterase residing in skin. Skin from pig ears was used and freshly excised into split-thickness membranes, which were divided and immediately stored at five different storage conditions: a) 4 °C overnight (fresh control), b) 4 °C for 4 days, c) and d) −20 °C for 6 weeks and one year, respectively, and e) −80 °C for 6 weeks. Based on the combined results, general trends are observed showing that fresh skin is associated with lower permeation of both model drugs and higher skin membrane electrical resistance, as compared to the other storage conditions. Interestingly, in the case of fresh skin, significantly lower amounts of MS are detected in the epidermis and dermis compartments, implying higher levels of ester hydrolysis of MS (i.e., higher esterase activity). In line with this, the concentration of salicylic acid (SA) extracted from the dermis is significantly higher for fresh skin, as compared to the other storage conditions. Nevertheless, for all storage conditions, substantial amounts of SA are detected in the receptor medium, as well as in the epidermis and dermis, implying that esterase activity is maintained to some extent in all cases. For AC, which is not expected to be affected by skin metabolism, freeze storage (protocols c-e) is observed to result in higher accumulation of AC in the epidermis, as compared to the case of fresh skin, while the AC concentration in dermis is unaffected. These observations can be rationalized primarily by the observed lower permeability of fresh skin towards this hydrophilic substance. Finally, a strong correlation between AC permeation and electrical skin resistance is shown for individual skin membranes irrespective of storage condition, while the corresponding correlation for MS is inferior. On the other hand, a strong correlation is shown for individual membranes between MS permeation and electrical skin capacitance, while a similar correlation for AC is lower. The observed correlations between drug permeability and electrical impedance open up for standardizing in vitro data for improved analysis and comparisons between permeability results obtained with skin stored at different conditions.

Diabetes mellitus differently affects electrical membrane properties of vagal afferent neurons of rats.

To study whether diabetes mellitus (DM) would cause electrophysiological alterations in nodose ganglion (NG) neurons, we used patch clamp and intracellular recording for voltage and current clamp configuration, respectively, on cell bodies of NG from rats with DM. Intracellular microelectrodes recording, according to the waveform of the first derivative of the action potential, revealed three neuronal groups (A0 , Ainf , and Cinf ), which were differently affected. Diabetes only depolarized the resting potential of A0 (from -55 to -44 mV) and Cinf (from -49 to -45 mV) somas. In Ainf neurons, diabetes increased action potential and the after-hyperpolarization durations (from 1.9 and 18 to 2.3 and 32 ms, respectively) and reduced dV/dtdesc (from -63 to -52 V s-1 ). Diabetes reduced the action potential amplitude while increasing the after-hyperpolarization amplitude of Cinf neurons (from 83 and -14 mV to 75 and -16 mV, respectively). Using whole cell patch clamp recording, we observed that diabetes produced an increase in peak amplitude of sodium current density (from -68 to -176 pA pF-1 ) and displacement of steady-state inactivation to more negative values of transmembrane potential only in a group of neurons from diabetic animals (DB2). In the other group (DB1), diabetes did not change this parameter (-58 pA pF-1 ). This change in sodium current did not cause an increase in membrane excitability, probably explainable by the alterations in sodium current kinetics, which are also induced by diabetes. Our data demonstrate that diabetes differently affects membrane properties of different nodose neuron subpopulations, which likely have pathophysiological implications for diabetes mellitus.

Neural modulation with photothermally active nanomaterials.

Modulating neural electrophysiology with high precision is essential for understanding neural communication and for the diagnosis and treatment of neural disorders. Photothermal modulation offers a remote and non-genetic method for neural modulation with high spatiotemporal resolution and specificity. This technique induces highly localized and transient temperature changes at the cell membrane interfaced with photothermally active nanomaterials. This rapid temperature change affects the electrical properties of the cell membrane or temperature-sensitive ion channels. In this Review, we discuss the fundamental material properties and illumination conditions that are necessary for nanomaterial-assisted photothermal neural excitation and inhibition. We examine how this versatile technique allows direct investigation of neural electrophysiology and signalling pathways in two-dimensional and three-dimensional cell cultures and tissues, and highlight the scientific and technological challenges in terms of cellular specificity, light delivery and biointerface stability on the road to clinical translation.

Electrical surface properties of nanoporous alumina membranes: influence of nanochannels' curvature, roughness and composition studied via electrokinetic experiments.

Among classical nanoporous oxide membranes, anodic aluminum oxide (AAO) membranes, made of non-connected, parallel and ordered nanochannels, are very interesting nanoporous model systems widely used for multiple applications. Since most of these applications involve local phenomena at the nanochannel surface, the fine description of the electrical surface behavior in aqueous solution is thus of primordial interest. Here, we use an original experimental approach combining several electrokinetic techniques (tangential and transverse streaming potential as well as electrophoretic mobility experiments) to measure the ζ-potential and determine the surface isoelectric points (IEPs) of several AAOs having different characteristic sizes and compositions. Using such an approach, all the different surfaces available in AAOs can be probed: outer surfaces (top and bottom planes), pore wall surfaces (i.e., inner surfaces) and surfaces created by the grinding of the AAOs. We find clear IEP differences between the outer, pore wall and ground surfaces and discuss these in terms of nanochannel and surface morphology (curvature and roughness) and of modifications of the chemical environment of the surface hydroxyl groups. These results highlight the heterogeneities between the different surfaces of these AAO membranes and emphasize the necessity to combine complementary electrokinetic techniques to properly understand the material, an approach which can be extended to many nanoporous systems.

Nano-MnO2/xanthan gum composite films for NO2 gas sensing

Nowadays, sensors based on polymers/nanostructured metal oxide composites have been investigated extensively because of their sensitivity to NO2 gas at ambient temperature. In this work, nanocomposite membranes of xanthan gum (XG) with different contents of MnO2 nanoparticles were prepared as a potential NO2 gas sensor operating at room temperature by a simple one-step oxidation-reduction reaction. The structural, morphological, thermal, and electrical properties of the composite membrane were investigated. The FT-IR results confirm the successful preparation of MnO2 through the oxidation of XG by KMnO4 and reveal further the structural changes of the XG/MnO2 nanocomposite upon its exposure to NO2 gas. The capping of the synthesized MnO2 nanoparticles by XG, the surface composition of the XG/MnO2 nanocomposite membranes, and the effect of NO2 gas on the surface composition was investigated using the XPS technique. The DC conductivity and dielectric loss of nanocomposites were higher than for neat XG. The conductivities of the nanocomposites XG/MO-4, XG/MO-4/low NO2, and XG/MO-4/high NO2 composites are half, one, and three orders of magnitude higher than that for pure XG revealing a transition from insulating to conductive properties. The results demonstrated that XG/MnO2 nanocomposite membranes are promising for potential applications in NO2 gas sensing.