Effects Of Pu-erh Tea Research Articles

Anti-Obesity, Anti-Atherosclerotic and Anti-Oxidant Effects of Pu-Erh Tea on a High Fat Diet-Induced Obese Rat Model

Pu-erh tea, a traditional Chinese beverage, has been believed to have many benefits to human health and without side effects. In this study, we systematically analyzed the main active components of Pu-erh tea and investigated its anti-obesity, anti-atherosclerotic and anti-oxidant effects using an obese rat model. Obesity was induced by feeding a high-fat diet and subsequently the experimental obese mice were fed with high-fat diet supplemented with low (2.5%), medium (5%) or high (7.5%) doses of Pu-erh tea powder for 6 weeks respectively. As result, the body weight gain of the rats was decreased by medium and high doses of Pu-erh tea treatments. Furthermore, the levels of serum total cholesterol (TC), triglyceride (TG) and atherosclerosis index (AI) were significantly lowered by Pu-erh tea compared to the control group. Conversely, high density lipoproteincholesterol (HDL-C) level of the rats was significantly elevated by Pu-erh tea treatments. In addition, Pu-erh tea treatments increased the activities of anti-oxidative enzymes such as superoxide dismutase (SOD) and glutathione peroxides (GSH-Px), whereas reduced the level of lipid peroxidation product malondialdehyde (MDA) in obese rats. Collectively, our find-ings revealed that Pu-erh tea exerts comprehensive benefits in anti-obesity, anti-atherosclerotic and anti-oxidant effects, therefore can be used as a promising functional food in obesity management.

Pu-erh Tea Has In Vitro Anticancer Activity in TCA8113 Cells and Preventive Effects on Buccal Mucosa Cancer in U14 Cells Injected Mice In Vivo

Pu-erh tea is a functional tea production in China. The functional effects should be proved. The oral cancer preventive and antimetastatic effects of Pu-erh tea in vitro and in vivo have been studied respectively. Pu-erh tea showed an inhibitory effect on human tongue carcinoma TCA8113 cells proliferation tested by 3-(4,5-Dimethyl-2-Thiazolyl)-2,5-Diphenyltetrazolium Bromide assay and induced TCA8113 apoptosis shown anticancer effect. The antimetastatic effect of Pu-erh tea in TCA8113 cells was proved by the decreasing of matrix metalloproteinases (MMPs) and increasing of tissue inhibitors of metalloproteinases (TIMPs) mRNA transcription. In the animal experiments, the tumor volumes and lymph node metastasis rates of Pu-erh tea-treated mice were smaller than control mice. Pu-erh tea reduced the levels of the serum proinflammatory cytokines interleukin (IL)-6, IL-12, tumor necrosis factor-α, and interferon-γ to a greater extent compared with the control mice, and the levels of 200 μg/mL treatment was more close to the normal mice than 100 μg/mL treated mice. Pu-erh tea also significantly induced apoptosis in tissues of mice (P < 0.05) by upregulating Bax and downregulating Bcl-2. These results demonstrate Pu-erh tea has cancer preventive and anti-metastatic effects on buccal mucosa cancer, the higher concentration get better efficiency.

0261 Hao Ling pu-erh tea attenuates lipid accumulation in primary culture rat hepatocytes

Tea is one of the most consumed beverages in the world and its healthpromoting effects have been widely investigated.Lipid-lowering effects of pu-erh tea have attracted growing interest.The importance of liver in lipid metabolism prompted us to investigate the lipid-lowering properties of pu-erh tea in rat primary culture hepatocytes.We tested the effect of a Hao Ling pu-erh tea extract (HLPT) and one of the major components of tea as Epigallocatechin-3-gallate (EGCG) which is largely recognized as a hypolipidemic molecule. HLPT: an infusion of Hao Ling pu-erh tea was lyophilized and quantified for its composition in catechins by LC-MS. 24h after seeding on collagen, rat hepatocytes in primary culture were treated for 24h with various concentrations of HLPT(100,200,400,600 μg/ml) and EGCG(30,100 uM) and compared to CyclosporinA (hyperlipidemic reference) and Clofibrate (hypolipidemic drug used in human)(n=3 in triplicate).Lipid droplet accumulation was measured by LipidTox staining and evaluated by fluorescence microscopy on an ArrayScanXTI high Content Analysis Reader(Cellomics Inc.). We found that HLPT significantly prevented hepatocyte lipid accumulation (−56%) and in the same proportion to Clofibrate.EGCG also significantly attenuated lipid accumulation (−19%) but less than HLPT. The main result of this study was to point out the major implication of liver cells in the hypolipidemic effects of HLPT.Moreover, we have shown here that this effect was partly due to the EGCG, well known for its antioxidant effects.However as we reported here that HLPT has a higher hypolipidemic effect than EGCG alone, which means that EGCG acts in synergy with other HLPT components such as theaflavins but this hypothesis has to be confirmed in further experiments.In future, the variation of expression of genes involved in lipid metabolism (THRSP, LXR, PPar-α) by qPCR induced by HLPT will allow us to improve the understanding of the effect induced by HLPT.

普洱茶调节apoE基因敲除小鼠血脂水平的研究

为了验证普洱茶对apoE基因敲除小鼠动脉粥样硬化的作用；本研究选取了40只4周龄雄性apoE基因敲除小鼠，按照体重随机分成降脂药组、模型组、生茶组、熟茶组4组外加10只正常对照组。用普洱茶汤进行干预性试验90天后，空腹12小时，摘眼球采血，采用全自动生化仪测血清中的总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)等血脂指标；试验结果显示：普洱茶(生、熟)均可显著控制小鼠体重增长(P 0.05)；普洱生茶可显著降低小鼠血清中TG、LDL-C的含量，升高HDL-C的含量(P )，可显著降低AI系数；因此可判定，普洱茶对apoE基因敲除小鼠的抗动脉粥样硬化有一定的调节作用。This study aimed to investigate the effects of Pu-erh tea on blood lipids in apoE-knockout mice. So we selected 4-week-old male apoE-gene knocked mice, and they were randomly divided into four groups: atherosclerosis group, fermenting Pu-erh Tea group, fermented Pu-erh Tea group and drug group. The all mice were used as the control. All mice were fed with normal chow diet. After 90 days, Vessels blood was collected for serum lipid, using automatic biochemical analyzed measuring serum the total clesterol (TC), triglycerides (TG), low-density lipoprotein (LDL-C), high-density lipoprotein (HDL-C), and other hyperlipidemia indicators. The result is that Pu-erh Health (cooked) tea cansignificantly increase control of body weight (P

Effects of Pu-erh Tea on Oxidative Damage and Nitric Oxide Scavenging

The effects of pu-erh tea, which is prepared by fermentation of tea, on oxidative damage and nitric oxide scavenging, compared with various other brands of tea were investigated. The total antioxidant activity was determined using the Trolox equivalent antioxidant capacity (TEAC) assay. The results showed that TEAC values of the 200 microg/mL water extracts of pu-erh tea (WEPT), green tea, oolong tea, and black tea were 86.3, 85.3, 87.4, and 80.3 (microg/mL), respectively, indicating that WEPT showed a significant antioxidant activity. WEPT, like green tea extract, oolong tea extract, and black tea extract, exhibited a remarkable protective effect in lipid (liposome) and nonlipid (protein and deoxyribose) model systems, implying that it is an inhibitor of lipid and nonlipid oxidative damage. It also exhibited metal-binding ability, reducing power, and scavenging effect for free radicals. Moreover, WEPT showed a decreasing effect on nitric oxide production of lipopolysaccharide-induced RAW 264.7 macrophages. In addition, the results revealed that epicatechin (EC), flavonoid, ascorbic acid, and polyphenolic compounds are present in WEPT, which may partially account for the protective effect on oxidative damage. Thus, WEPT may have potential as an antioxidant and as a nitric oxide scavenging agent.

S87 The hypolipidemic and antioxidative effects of pu-erh tea

S87 The hypolipidemic and antioxidative effects of pu-erh tea

Effects of pu-erh tea on lipid metabolism in rats.

The effect of water extracts of Pu-Erh tea (products of Yunnan district, China, preserved for 2 or 20 years) and of green tea (products of Shizuoka prefecture, Japan) on lipid level, tissue weight, lipoprotein lipase (LPL) and adrenalin-induced lipolytic (AIL) activity in rats were examined. Female Wistar rats (8 weeks old) were fed a diet containing 1% cholesterol and given the above tea extracts in drinking water for 8 or 16 weeks ad libitum. The levels of plasma cholesterol ester in rats given Pu-Erh tea or green tea were significantly lower than those of control rats after 6-8 weeks, though the difference became smallar after 10 weeks. The triglyceride (TG) level in plasma was also low in rats given Pu-Erh tea for 16 weeks. This effect on TG was not observed in the case of green tea. Among 6 organs or tissues of rats examined, the weight of abdominal adipose tissue was significantly lower in rat fed Pu-Erh tea for 16 weeks. The LPL activity in abdominal adipose tissue tended to be low (though not statistically significant), while the activity of AIL was significantly elevated in rats given Pu-Erh tea for 8 or 16 weeks. A negative correlation was observed between AIL activity and the ratio of adipose tissue/body weight. These data suggest that the successive administration of Pu-Erh tea could stimulate the degradation of TG in adipose tissue and thereby decrease its weight.