Effects Of Ozone Research Articles

Accelerated aging and altered subclinical response to ozone exposure in young, healthy adults

AbstractOzone exposure induces a myriad of adverse cardiopulmonary outcomes in humans. Although advanced age and chronic disease are factors that may exacerbate a person’s negative response to ozone exposure, there are no molecular biomarkers of susceptibility. Here, we examine whether epigenetic age acceleration (EAA) is associated with responsiveness to short-term ozone exposure. Using data from a crossover-controlled exposure study (n = 17), we examined whether EAA, as measured in lung epithelial cells collected 24 h after clean air exposure, modifies the observed effect of ozone on autonomic function, cardiac electrophysiology, hemostasis, pulmonary function, and inflammation. EAA was assessed in lung epithelial cells extracted from bronchoalveolar lavage fluids, using the pan-tissue aging clock. We used two analytic approaches: (i) median regression to estimate the association between EAA and the estimated risk difference for subclinical responses to ozone and (ii) a block randomization approach to estimate EAA’s effect modification of subclinical responses. For both approaches, we calculated Fisher-exact P-values, allowing us to bypass large sample size assumptions. In median regression analyses, accelerated epigenetic age modified associations between ozone and heart rate–corrected QT interval (QTc) (${{\hat \beta }}$= 0.12, P-value = 0.007) and between ozone and C-reactive protein (${{\hat \beta }}$ = −0.18, P = 0.069). During block randomization, the directions of association remained consistent for QTc and C-reactive protein; however, the P-values weakened. Block randomization also revealed that responsiveness of plasminogen activator inhibitor-1 (PAI-1) to ozone exposure was modified by accelerated epigenetic aging (PAI-1 difference between accelerated aging-defined block groups = −0.54, P-value = 0.039). In conclusion, EAA is a potential biomarker for individuals with increased susceptibility to ozone exposure even among young, healthy adults.

Antibacterial Effect of Ozone on Cariogenic Bacteria and Its Potential Prejudicial Effect on Dentin Bond Strength-An In Vitro Study.

Ozone is increasingly utilized in dental caries treatment due to its antibacterial properties. In a context of limited studies and no consensus on protocols, this research aims to assess ozone's antibacterial efficacy on cariogenic bacteria and its potential adverse impact on dentin bond strength. Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei, and Actinomyces naeslundii suspensions were exposed to 40 μg/mL of ozone gas and 60 μg/mL of ozonated water (80 s) via a medical ozone generator. Negative and positive control groups (chlorhexidine 2%) were included, and UFC/mL counts were recorded. To examine microtensile bond strength (µTBS), 20 human molars were divided into four groups, and class I cavities were created. After ozone application, samples were restored using an etch-and-rinse and resin composite, then sectioned for testing. The SPSS v. 28 program was used with a significance level of 5%. The µTBS results were evaluated using one-way ANOVA, Tukey HSD, and Games-Howell. Bacterial counts reduced from 106 to 101, but dentin µTBS was significantly impacted by ozone (ANOVA, p < 0.001). Despite ozone's attractive antibacterial activity, this study emphasizes its detrimental effect on dentin adhesion, cautioning against its use before restorative treatments.

Evaluation of the restorative effect of ozone and chitosan-hyaluronic acid with and without mesenchymal stem cells on wound healing in rats.

This study evaluated the effect of ozone, chitosan-hyaluronic (Cs-HA) acid and mesenchymal stem cells (MSCs) on wound healing in rats. A total of 64 rats were randomly divided into four groups: control, ozone, Cs-HA+ozone and Cs-HA+ozone+MSCs. A 5mm full-thickness wound was created on the back of each rat. The wound area was measured macroscopically on days 3, 5, 9 and 14. Tissue sections were prepared for histopathological evaluation of inflammation, collagen arrangement, neovascularization and epithelial tissue rearrangement. Macroscopic assessment showed differences in wound area on days 5, 9 and 14. Histopathological examination showed that the Cs-HA+ozone+MSCs and Cs-HA+ozone groups had significantly higher vascularization on day 3 compared to the ozone-treated and control groups. All treatment groups had significantly better collagen arrangement than the control group. On day 5, no significant difference was observed between different groups. On day 9, the inflammation level in the Cs-HA+ozone+MSCs group was significantly lower than in the other groups. All treatment groups had significantly better vascularization compared to the control group. On day 14, the rate of inflammation was significantly lower in the treatment groups than in the control group. Significantly higher collagen arrangement levels were observed in the Cs-HA+ozone and Cs-HA+ozone+MSCs groups compared to the control and ozone groups. All treatment groups had significantly better epithelial tissue rearrangement than the control group. Overall, the results of this study indicated that treatment with ozone, Cs-HA acid, Cs-HA and MSCs accelerated wound healing in rats. The effect of using Cs-HA acid with mesenchymal cells was better than the other types of treatment. Larger clinical trials are needed to assess these factors for improving chronic wound treatment.

ТЕОРЕТИЧЕСКИЕ ПРЕДПОСЫЛКИ ОПРЕДЕЛЕНИЯ РАЗМЕРА И ОБЪЁМА РАСПРОСТРАНЕНИЯ ОЧАГА ОЗОНА В ЗЕРНОВОМ ВОРОХЕ И ОПТИМИЗАЦИЯ ВРЕМЕНИ ОЗОНИРОВАНИЯ

One of the most promising methods for processing agricultural products today is the ozonation process. The effect of ozone on grain will allow it to be disinfected and stimulate the growth processes of the seed, without causing harmful emissions into the environment. The purpose of the research was to find the optimal time for ozonation of grain and seeds depending on the processing speed of a certain volume of grain material. To achieve it, numerical optimization methods were used, namely the patternsearch global search method, which will determine the most rational ozonation regime for given conditions, were used to achieve it. The source of ozone propagation in a grain heap during ozonation for 10...60 minutes is close in shape to an ellipsoid, but has a slightly different mathematical description. The mathematical models obtained by interpolation make it possible to determine the minimum ozonation time and the maximum speed of ozone treatment per unit volume of a grain heap. It was revealed that increasing the processed volume of grain due to the longer duration of the operation does not make sense, as it will contribute to an increase in energy and cost costs. Under given conditions, an ozonation time of 1270 seconds provides a maximum ozone treatment rate of 3059 mm3/s per unit volume of grain. Optimization will make it possible to ozone the maximum volume of a grain storage facility as quickly as possible at minimal cost. The shortest processing time per unit volume under these conditions was 0.000327 s/mm3. Other ozonation parameters will have different optimal values, therefore the proposed methodology is the basis of an appropriate computer program that will automatically determine the optimal ozonation regime for specific conditions.

Effects of climate change and ozone on vegetation phenology on the Tibetan Plateau

The vegetation phenology, encompassing the start (SOS) and end (EOS) of the growing season on the Tibetan Plateau, has been significantly impacted by global climate change. Furthermore, ozone (O3) has gradually become the main pollutant in this region, substantially influencing carbon cycle and ecosystems on Earth. While ongoing studies have focused mainly on the implications of climate parameters, including temperature, precipitation, and radiation, the effects of O3 on the SOS and EOS remain unclear. Here, we compared the responses and sensitivities of the SOS and EOS to both climatic factors and O3 in this region. With the use of partial correlation analysis, we found that increased precipitation was the most important factor influencing the SOS and caused earlier occurrence (4.8 % vs. 21.9 %) for most plant functional types. In comparison, temperature only dominated in shrublands. In particular, we found that the EOS responded comparably to climatic factors with similar proportions between advancing and delaying patterns. However, higher O3 levels consistently advanced the EOS for almost all plant functional types and was the main factor controlling EOS variations based on the sensitivity analysis. Our results emphasized that O3 pollution should be considered for obtaining better phenological forecasts and determining the impacts of the environment and atmospheric composition on carbon sequestration in terrestrial ecosystems.

Drying Over Eastern China Driven by the Depletion of Arctic Stratospheric Ozone During Boreal Spring

AbstractGiven the significant importance of spring precipitation for agricultural production in China and the presence of the spring predictability barrier, scientists have dedicated extensive efforts to understand the factors influencing spring precipitation variability and explore new predictors. However, the effects of Arctic stratospheric ozone (ASO) on precipitation in China during boreal spring, if any, and the underlying mechanisms remain unclear. We found the robust influences of March ASO on the differences in the precipitation and evaporation in April over Eastern China during 1980–2020. When ASO decreases in March, it tends to result in a higher and colder tropopause in the polar, a stronger subtropical jet stream, an intensified local Hadley circulation accompanied by anomalous downward motion over Eastern China, and consequently, drying in this region, and vice versa. These findings suggest that the likelihood of April moistening over East Asia may be potentially predicted by employing the ASO index.

The effect of water ozonation in the presence of microplastics on water quality and microplastics degradation

The occurrence of microplastics in water treatment plants poses a concern for the quality of treated water. When microplastics pass through water treatment plants, they can be oxidized, changing their surface characteristics and the quality of the treated water. This work aimed to investigate the impact of ozone and the association of ozone and hydrogen peroxide on five different microplastic particles that are commonly detected in water samples. The changes in the concentration of total organic carbon and the change in the pH of the water, the leaching of phthalic acid esters, as well as the changes in size and chemical changes in the structure of the tested microplastics were evaluated. The influence of ozonation time, water pH, and type of microplastics, as well as the influence of the addition of hydrogen peroxide, was analyzed. The effect of ozonation was an increase in DOC values ranging from 0.8 to 28 mg/L. The eluting substances included phthalic acid esters, plasticizers with a proven negative impact on organisms. The percentage loss of the surface area of the microplastic was in the range of 1.3 to 26.7 %. PE was more susceptible to degradation. LDIR analyzes were carried out to investigate the effect of O3 and O3/H2O2 treatments on the surface of MPs. This study demonstrated that MPs could change their physical and chemical characteristics if they are subjected to oxidation processes used in water treatment plants. The parameters of purified water change to unfavorable ones due to the leaching of additives. Although much research has been conducted on the occurrence of microplastics in treated water, awareness needs to be raised about the interactions between plastic particles and water treatment technology processes.

Comprehensive Assessment of Environmental Factors Influencing Crop Yields: Ozone, CO2, And Aerosols

The research explored how air pollution, particularly high ozone levels, negatively impacts sensitive crops like wheat, rice, and soybeans, posing significant threats to global food security. It emphasized the long-overlooked destructive effects of ozone and emphasized the immediate need for strategies to counter these impacts amid climate change. Furthermore, the study examined the crucial role of increased carbon dioxide (CO2) levels on C4 crops, essential for global food security and biofuel production. Contrary to expectations, it challenged the belief that C4 crops inherently benefit from elevated CO2 levels in terms of enhanced photosynthesis. The study urged more experiments under changing climate conditions to refine predictions regarding crop yields. It emphasized the importance of focusing on ozone reduction, understanding how C4 crops respond to increased CO2, and assessing aerosol effects on specific crops. These insights aim to inform policies and strategies that can safeguard food security amidst rapid global economic growth and the challenges posed by climate changet.

Effects of Air Pollution, Ozone, and Sulfur Dioxide on Kidney Oxidative-Stress Enzymes, Histopathology, and Apoptosis Gene Expressions in Rats

Effects of Air Pollution, Ozone, and Sulfur Dioxide on Kidney Oxidative-Stress Enzymes, Histopathology, and Apoptosis Gene Expressions in Rats

In Vitro Investigation of the Antimicrobial Potential and Cell Viability of Different Dosages of Ozone Therapy

ABSTRACT The aim of the study was to evaluate and compare, in vitro, the cytotoxicity of different applied ozone dosages in keratinocyte (NOK) and fibroblast (L929) cell lines, as well as the antimicrobial potential for Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). NOK and L929 cells and strains of gram-positive bacteria S. aureus and gram-negative bacteria E. coli were divided into seven experimental groups: ozone at doses from 0, 0.005, 0.01, 0.02, 0.03, 0.04 and 0.05 mg/L. The effect of ozone on cell viability/cytotoxicity was determined using the alamarBlue® assay, the Live/Dead® assay and the morphometric analysis of the percentage of live and dead cells. The results show that applied ozone dosages of 0.005, 0.01 and 0.02 mg/L showed the highest viability values of NOK and L929 cells, as well as a higher density and percentage of live cells in the 72-hour experimental. However, no difference was observed in the percentage of dead cells between the experimental groups. Furthermore, ozone promoted a reduction in CFUs at all tested dosages. With the results obtained in the present study, it is possible to confirm the restorative potential of ozone therapy in a dose-dependent response, as well as its antimicrobial potential.

Modeling the effects of tropospheric ozone on the growth and yield of global staple crops with DSSAT v4.8.0

Abstract. Elevated surface ozone (O3) concentrations can negatively impact growth and development of crop production by reducing photosynthesis and accelerating leaf senescence. Under unabated climate change, future global O3 concentrations are expected to increase in many regions, adding additional challenges to global agricultural production. Presently, few global process-based crop models consider the effects of O3 stress on crop growth. Here, we incorporated the effects of O3 stress on photosynthesis and leaf senescence into the Decision Support System for Agrotechnology Transfer (DSSAT) crop models for maize, rice, soybean, and wheat. The advanced models reproduced the reported yield declines from observed O3-dose field experiments and O3 exposure responses reported in the literature (O3 relative yield loss RMSE &lt;10 % across all calibrated models). Simulated crop yields decreased as daily O3 concentrations increased above 25 ppb, with average yield losses of 0.16 % to 0.82 % (maize), 0.05 % to 0.63 % (rice), 0.36 % to 0.96 % (soybean), and 0.26 % to 1.23 % (wheat) per ppb O3 increase, depending on the cultivar O3 sensitivity. Increased water deficit stress and elevated CO2 lessen the negative impact of elevated O3 on crop yield, but potential yield gains from CO2 concentration increases may be counteracted by higher O3 concentrations in the future, a potentially important constraint to global change projections for the latest process-based crop models. The improved DSSAT models with O3 representation simulate the effects of O3 stress on crop growth and yield in interaction with other growth factors and can be run in the parallel DSSAT global gridded modeling framework for future studies on O3 impacts under climate change and air pollution scenarios across agroecosystems globally.

Effect of tropospheric ozone and its protectants on gas exchange parameters, antioxidant enzymes and quality of Garlic (Allium sativum. L).

An experimental study was conducted to assess the detrimental effect of ground-level ozone (O3) on garlic physiology and to find out appropriate control measures against ground-level O3, at TNAU-Horticultural Research farm, Udhagamandalam. Elevated ground ozone levels significantly decreased garlic leaf chlorophyll, photosynthetic rate, stomatal conductance, total soluble solids and pungency. The garlic chlorophyll content was highest in ambient ozone level and lowest in elevated ozone@200ppb, highest stomatal conductance was recorded in ambient ozone with foliar spray of 3%Panchagavya, and the lowest was observed in elevated ozone@200ppb. Since the elevated O3 had reduced in garlic photosynthetic rate significantly the lowest was observed in elevated O3@200ppb and the highest photosynthetic rate was observed in ambient Ozone with foliar spray 3% of panchagavya after a week. The antioxidant enzymes of garlic were increased with increased concentration of tropospheric ozone. The highest catalase (60.97µg of H2O2/g of leaf) and peroxidase (9.13 ΔA/min/g of leaf) concentration was observed at 200ppb elevated ozone level. Garlic pungency content was highest in ambient ozone with foliar spray of 0.1% ascorbic acid and the lowest was observed under elevated O3@200ppb. Highest total soluble solids were observed in ambient ozone with foliar spray of 3%Panchagavya and the lowest observed in elevated ozone@200ppb. Thus, tropospheric ozone has a detrimental impact on the physiology of crops, which reduced crop growth and yield. Under elevated O3 levels, ascorbic acid performed well followed by panchagavya and neem oil. The antioxidant such as catalase and peroxidase had positive correlation among themselves and had negative correlation with chlorophyll content, stomatal conductance, photosynthetic rate, pungency and TSS. The photosynthetic rate has high positive correlation with chlorophyll content, pungency and TSS. Correlation analysis confirmed the negative effects of tropospheric ozone and garlic gas exchange parameters and clove quality. The ozone protectants will reduce stomatal opening by which the entry of O3 in to the cell will be restricted and other hand they also will alleviate ROS and allied stresses.

Ozonation effects on structural and functional characteristics of starch

Ozone oxidation leads to the substitution of hydroxyl groups in the starch molecules with carboxyl or carbonyl groups, thereby increasing the degree of substitution. A thorough examination of how ozone treatment impacts the composition of various cereal crops, including wheat, rice, and maize, is provided. This review explores the repercussions of ozone on starch rheology, germination ability, and storage stability.The present review also explores the textural qualities of food products such as bread, noodles, and cakes prepared with ozonized grains. Ozone treatment is found to significantly reduce swelling capacity and solubility of natural starch, and differential scanning calorimetry reveals that the gelatinization and retrogradation temperature profiles of oxidized starches remain comparable to those of native starch. The evidence that ozone treatment holds substantial promise for enhancing the functionality of starch in various applications while simultaneously upholding the essential standards of food safety has also been explained in detail. The findings synthesized in this review shed light on the intricate interplay between ozone and starch, providing valuable insights for both the scientific community and the food industry.

Retracted: Effects of Ozone on Hippocampus BDNF and Fos Expressions in Rats with Chronic Compression of Dorsal Root Ganglia.

[This retracts the article DOI: 10.1155/2021/5572915.].

Air pollution and upper respiratory diseases: an examination among medically insured populations in Wuhan, China.

Multiple evidence has supported that air pollution exposure has detrimental effects on the cardiovascular and respiratory systems. However, most investigations focus on the general population, with limited research conducted on medically insured populations. To address this gap, the current research was designed to examine the acute effects of inhalable particulate matter (PM2.5 and PM10), nitrogen dioxide (NO2), ground-level ozone (O3), and sulfur dioxide (SO2) on the incidence of upper respiratory tract infections (URTI), utilizing medical insurance data in Wuhan, China. Data on URTI were collected from the China Medical Insurance Basic Database for Wuhan covering the period from 2014 to 2018, while air pollutant data was gathered from ten national monitoring stations situated in Wuhan city. Statistical analysis was performed using generalized additive models for quasi-Poisson distribution with a log link function. The analysis indicated that except for ozone, higher exposure to four other pollutants (NO2, SO2, PM2.5, and PM10) were significantly linked to an elevated risk of URTI, particularly during the previous 0-3 days and previous 0-4 days. Additionally, NO2 and SO2 were found to be positively linked with laryngitis. Furthermore, the effects of air pollutants on the risk of URTI were more pronounced during cold seasons than hot seasons. Notably, females and the employed population were more susceptible to infection than males and non-employed individuals. Our findings gave solid proof of the link between ambient air pollution exposure and the risk of URTI in medically insured populations.

Medical Ozone: A Redox Regulator with Selectivity for Rheumatoid Arthritis Patients.

Rheumatoid arthritis (RA) and osteoarthritis (OA) are the most common arthritic diseases. Medical ozone has demonstrated its effectiveness in combination therapy with methotrexate or non-steroidal anti-inflammatory drugs for RA and OA, respectively. Although RA and OA have been compared from different points of view, few studies have considered their redox status in spite of the oxidative processes that are involved in both diseases. The aim of this study was to compare RA with OA, evaluating their redox status and the effects of ozone on their clinical response to combined therapy with ozone. The redox status of 80 patients was determined: antioxidant defenses, injury markers, two subjective variables (pain and disability), and levels of antibodies against cyclic citrullinated peptides were evaluated. Oxidative stress and clinical response to combined therapy with ozone was higher than in the case of RA. After medical ozone treatment, there was an increase in antioxidant defense and a decrease in injury markers as well as pain, disability, and autoantibody concentrations. Redox biomarkers were able to differentiate between both arthritic diseases and combined therapy with ozone (methotrexate + ozone), showing a therapeutic selectivity for RA in comparison with OA.

The Influence of Ozone on the Flame Propagation Behavior of n-Heptane/air and i-Octane/air Premixed Flame in an Engine-Relevant Environment

ABSTRACT The promotional effect of ozone on lean premixed spherical flame propagation in an engine-relevant, high-temperature, high-pressure environment was investigated using an optically accessible rapid compression and expansion machine (RCEM). The laminar burning velocity, burned gas Markstein length, and initial flame development time based on mass fraction burned (MFB), were examined for n-heptane/air and i-octane/air premixture (φ = 0.55) with 15 [ppm] ozone, through self-emission observation of a propagating flame with a high-speed camera and combustion pressure analysis. The results showed that ozone reduced the initial flame development time and increased the laminar burning velocity for n-heptane/air premixture under temperature conditions in which the low-temperature oxidation (LTO) reaction was more clearly pronounced; but ozone did not enhance either the initial flame development or the laminar burning velocity in the case of i-octane/air premixture, where the LTO reaction was weak owing to the fuel molecular structure, even under the same temperature conditions. These results suggest that the enhancement of the LTO reaction by ozone, and the subsequent generation of thermal energy and CO radicals through an H2O2 loop reaction in the pre-flame region, were responsible for the increase in the laminar burning velocity. Furthermore, ozone increased the burned gas Markstein length, flame thickness, and Zeldovich number of the n-heptane/air mixture under the temperature conditions in which the laminar burning velocity was enhanced by ozone. This suggests that the chemical reaction in the reaction zone was more active in response to the increase in flame temperature.

Gamma irradiation and ozone application as preservation methods for longer-term storage of bee pollen

Bee pollen is a healthy product with a good nutritional profile and therapeutic properties. Its high moisture content, however, promotes the growth of bacteria, molds, and yeast during storage commonly result in product degradation. Therefore, the aim of this study is to assess the effectiveness of gamma irradiation (GI) and ozone (OZ) as bee pollen preservation methods for longer storage time, as well as whether they are influenced by pollen species. To do that, GI at a dosage of 2.5, 5.0, and 7.5 kGy was applied at a rate of 0.68 kGy/h and OZ application at a concentration of 0.01, 0.02, and 0.03 g/m3 was applied for one time for 6 h, to Egyptian clover and maize bee pollen, then stored at ambient temperature for 6 months. We then determined the total phenolic content (TPC) and antioxidant activity of treated and non-treated pollen samples at 0, 3, and 6 months of storage. Total bacteria, mold, and yeast count were also evaluated at 0, 2, 4, and 6 months. Statistical analyses revealed that, TPC, antioxidant, and microbial load of both clover and maize pollen samples were significantly (p < 0.05) affected by both treatment and storage time and their interaction. Both methods were extremely effective at preserving the antioxidant properties of pollen samples after 6 months of storage at room temperature. Furthermore, the highest concentrations of both GI and OZ applications completely protected pollen samples from mold and yeast while decreasing bacterial contamination. GI at the highest dose (7.5 KGy) was found to be more effective than other GI doses and OZ application in preserving biologically active compounds and lowering the microbial count of pollen samples for 6 months. As a result, we advise beekeepers to use GI at this dose for longer-term storage.

Toxicity of commercial and pure forms of three nonsteroidal anti-inflammatory drugs in Xenopus laevis embryos before and after ozonation

In this study, the toxic and teratogenic effects of three commercial drugs and their active ingredients on Xenopus laevis embryos before and after ozonation were evaluated using the Frog Embryos Teratogenesis Assay–Xenopus (FETAX). First, the median lethal concentration (LC50) and, if data were available, the median effective concentration, teratogenic index and minimum growth inhibitory concentration were determined for each drug substance without ozonation. Then, the active substance amounts of three selected nominal concentrations (LC50/2, LC50, and LC50×2) of each test substance before ozonation were measured by HPLC analysis and the toxicity of these substances was evaluated after 2, 3, 4, and 5 h of ozonation. In addition, degradation products that may occur during ozonation were evaluated by LC-MS analysis. The 96-h LC50s of Dolphin–diflunisal, Dichloron–diclofenac sodium, and Apranax–naproxen drug–active substance pairs were determined to be 22.3 and 11.1, 25.7 and 18.7, and 47.8 mg active substance/L and 45.3 mg/L, respectively. According to the FETAX test results, the Dolphin–diflunisal drug–active ingredient pair did not cause growth retardation in exposed embryos. Dichloron–diclofenac sodium and Apranax–naproxen drug–active ingredient pairs were both teratogenic and growth inhibitory. In the second stage of the study, in which the effectiveness of ozonation in eliminating the toxic effects of drugs is evaluated, it is seen that ozonation is partially successful in eliminating the toxic effects of Dolphin–diflunisal and Dichloron–diclofenac sodium pairs, but insufficient for eliminating the effects of the Apranax–naproxen pair.

Potential of gaseous ozone to inhibit Aspergillus flavus and detoxify aflatoxin B1 in agar and broth media (solid and liquid food states) at different growth phases

Agricultural food crops are prone to Aspergillus flavus contamination during post-harvest and storage stages. Their consumption is toxic due to aflatoxin production, a potent carcinogenic and immunosuppressive agent. Present study explored the ozone's effect as a function of food media states (solid and liquid) to inhibit Aspergillus flavus under three modes of treatment (0th-day, Every-day and Nth-day ozone treatment), that adds novelty to the work. Results showed that ozone concentration to treat Aspergillus flavus and aflatoxins is higher in liquid than solid food state. Further, ozone treatment affected the intactness of fungal spores and inhibited 100% conidial growth. It also exhibited 88 %% inhibition and 90% reduced biomass in solid and liquid food states. Effectiveness of ozone towards inhibiting Aspergillus flavus and aflatoxin was in the following order: Nth -day treatment > Every-day treatment > 0th -day ozone treatment. Further, growth studies showed ozone treated cultures exhibit delayed sporulation and aflatoxin production by 72 h. when cultured on fresh media. Additionally, ozone purging into extracted aflatoxin led to > 99% degradation. The above results show the significance of ozone technology in increasing the shelf-life of post-harvested food crops. Further, it can be used as a disinfectant in the food industry to treat contaminated food crops/liquid products by detoxifying aflatoxins.