Subject. The article investigates a set of qualitative and quantitative indicators of the transport infrastructure development in the constituent entities of the Russian Federation. Objectives. We aim to assess the state and the level of transport infrastructure development in the constituent entities of the Russian Federation, which includes the analysis of qualitative and quantitative indicators of transport infrastructure, and the calculation of composite indices. Methods. The study employs methods of economic, statistical analysis and synthesis, comparison, the Ward’s method. The theoretical basis rests on the works by economists on the problems of transport security, spatial development, and the impact of transport infrastructure on the dynamics of economic growth. Results. We present the criteria, assessment methodology and results of assessing the index of the level of transport infrastructure development in the context of the constituent entities of the Russian Federation. The offered methodological toolkit was tested on materials of the Russian Federation, based on data for 2014–2019, namely, the assessment and ranking of the subjects of the Russian Federation by the level of transport infrastructure development. As a result of clustering the constituent entities of the Russian Federation, using the Ward’s method, four groups of regions have been identified in terms of the level of transport infrastructure development. Conclusions. The practical use of our approach will enable to form a basis for making management decisions, aimed at increasing the efficiency of activities in planning the development of territories and assessing the socio-economic effects of infrastructure projects.