Effect Of Green Silver Nanoparticles Research Articles

Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro.

IntroductionExtensive use of metallic nanomaterials in different areas of agriculture and commercial products induce significant harmful effects on human health and the environment. In the current study, we synthesized an eco-friendly approach silver nanoparticles (AgNPs) using root extracts of Beta vulgaris L.MethodsThe synthesized green silver nanoparticles (gAgNPs) were characterized by dynamic light scattering (DLS) and high-resolution transmission electron microscope (HR-TEM). The gAgNPs had a round shape and the mean size was 20−50 nm. The cytotoxic effects of gAgNPs were determined in human hepatic normal (CHANG) and cancer (HUH-7) cells by using tetrazolium salt (MTT) and lactate dehydrogenase (LDH) assays for 24 h.Results and DiscussionIt was clear from the observations of this experiment that higher concentrations of gAgNPs reduce cell viability. The production of reactive oxygen species (ROS) was evaluated by using DCFDA. The gAgNPs induced more ROS in the HuH-7 cells than in the CHANG cells. The fragmentation of DNA was evaluated by alkaline single-cell gel electrophoresis and the maximum DNA strand breakage was found at a higher concentration exposure of gAgNPs for 24 h. It is important to notice that the HuH-7 cells showed an increased sensitivity to gAgNPs than the CHANG cells. The apoptotic and necrotic effects of gAgNPs on both the cells were evaluated using annexin-V-FITC and propidium iodide staining. An increased count of apoptotic and necrotic cells was found following a higher concentration exposure of gAgNPs. The apoptotic protein expression in these cells due to gAgNPs exposure was determined using immunoblotting techniques and the level of Bcl2 was decreased. However, the expression of BAX and protein was increased in both cells.ConclusionTherefore, it can be concluded that higher concentrations of gAgNPs may induce significant cytotoxicity and cause DNA damage and apoptosis.

Effect of Green Silver Nanoparticles Embedded in Irradiated Sodium Alginate /Poly Acrylamide on Removal of Dye Wastes

The toxins generated by waste dyes from several industrial sectors in water bodies are hard to evacuate by ordinary water treatment procedures. This study discusses the effect of silver nanoparticl...

Cytotoxic and mutagenic effects of green silver nanoparticles in cancer and normal cells: a brief review

Applications of silver nanoparticles (AgNPs) have revolutionized the medicinal industry. Due to small size (1–100 nm) these nanoparticles are able to cross cell and nuclear membrane and induce cyto/genotoxicity. Green synthesized (plant based) AgNPs (GSAgNPs) have emerged as alternative antimicrobial agent to chemically synthesized nanoparticles assuming their methods of synthesis will be environment friendly and non toxic. In this review we report that the GSAgNPs were found to be cytotoxic and genotoxic to different cancer cell lines. They affected cell proliferation caused apoptosis and cell death of human cancer cells but did not cause such damages to normal human peripheral blood lymphocyte cells. This property can be utilized in cancer therapeutics to overcome the major drawbacks of chemotherapeutics with surface modifications of the nanoparticles by adding functionalized AgNPs.

Effects of Green Silver Nanoparticles on Soil Quality and Induced Germination: A Future Alternative Fertilizer or Environmental Toxicant?

Silver nanoparticles (AgNP) synthesized from It influenced the inherent soil properties like bulk density (BD), water holding capacity (WHC), available N, P, K, urease, phosphatase activity and TOC. The apparent increment WHC, N, P, K, urease, and phosphatase in soil were observed whereas reduction of BD was noticed. Due to application of nanosolutions the pH of the soil shifted towards neutrality from 0 to 60 days. Moreover, they also did not have any toxicity upon plant as well as earthworm ecosystem.

An investigation on the antibacterial, cytotoxic, and antibiofilm efficacy of starch-stabilized silver nanoparticles

The increased emergence of drug resistant microbes creates a major challenge to the scientific community for successful development of effective therapeutics. The antimicrobial activities of silver ions are well known, but limited information is available on the effects of green silver-nanoparticles (AgNPs) on human pathogens. In this article, we evaluated the antibacterial activity of starch-stabilized AgNPs against a panel of human pathogens commonly associated with air, water and food borne infections. The shape and size distribution of AgNPs were characterized by transmission electron microscopy. We showed that AgNPs were more effective against Gram-positive and Gram-negative pathogens as compared with acid-fast bacteria. AgNPs were not cytotoxic to macrophages at the bactericidal concentration and can augment intracellular killing potential of macrophages. Furthermore, we showed that AgNPs disrupt biofilm formation and exhibit better antibacterial activity compared to human cationic antimicrobial peptide LL-37. In summary, our data suggest AgNPs as a promising template for the design of novel antibacterial agents. From the Clinical EditorIn this fundamental study, the significance of starch-stabilized AgNP-s is studied as antimicrobial agents on a variety of human pathogens. AgNP-s are expected to become universally utilized agents in our efforts to prevent and fight infections.