Effects Of Flavonoid Apigenin Research Articles

The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of Prmt7/Akt3 Pathway.

Apigenin, a common dietary flavonoid abundantly present in a variety of fruits and vegetables, has promising anticancer properties. As an effector of apigenin in myoblasts, protein arginine methyltransferase 7 (Prmt7) is required for male germ cell development. However, whether apigenin may influence male reproductive health through Prmt7 is still unclear. To this end, mouse spermatogonia were treated with different concentrations (2.5 to 50 μM) of apigenin for 48 h, which showed that apigenin could cause reduced cell proliferation in conjunction with longer S phase and G2/M phase (with concentrations of 10 and 20 μM, respectively), and increased apoptosis of spermatogonia (with concentration of 20 μM). Reduced Prmt7 expression was found in 20 μM apigenin-treated spermatogonia. Moreover, siRNA-induced Prmt7 knockdown exhibited similar influence on spermatogonia as that of apigenin treatment. In mechanistic terms, transcriptome analysis revealed 287 differentially expressed genes between Prmt7-downregulated and control spermatogonia. Furthermore, rescue experiments suggested that the effects of apigenin on spermatogonia might be mediated through the Prmt7/Akt3 pathway. Overall, our study supports that apigenin can interfere with mouse spermatogonial proliferation by way of the downregulated Prmt7/Akt3 pathway, which demonstrates that the concentration should be taken into account in future applications of apigenin for cancer therapy of men.

Therapeutic potentials of the natural plant flavonoid apigenin in polycystic ovary syndrome in rat model: via modulation of pro-inflammatory cytokines and antioxidant activity

Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age, which is associated with increased androgens, chronic inflammation, and oxidative stress. Current research aims at determining the effect of flavonoid apigenin on the level of antioxidant and anti-inflammatory factors in ovarian tissue of rats with PCOS. In this study, 32 female Wistar rats were divided into four groups (n = 8): control, PCOS control, and treated groups (20 and 40 mg/kg apigenin). After 21 days of intervention, the serum levels of sex hormones and gonadotropins were measured. The ovarian tissue was removed for biochemical and histological studies. Research findings indicated that apigenin causes significant decrease in estrogen, testosterone levels, LH and LH to FSH ratio, and significant increase in progesterone and FSH levels in serum of treated groups compared to PCOS control group. Different doses of apigenin significantly decreased the inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and total oxidative status (TOS). The total antioxidant capacity (TAC) and superoxide dismutase (SOD) activity significantly increased in treated groups compared to the PCOS control group. The histological results indicated that the number of cysts and theca layer thickness significantly decreased and the number of corpora lutea and granulosa layer thickness significantly increased in the rats receiving the apigenin as compared to the PCOS control group. Flavonoid apigenin through antioxidant and anti-inflammatory properties can be used as an alternative method for treating patients with PCOS.

Abstract C7: Inhibitory effects of flavonoid Apigenin by oral administration on angiogenesis of ovarian cancer through decrease VEGF expression in mouse model.

Abstract Diet can play a vital role in cancer prevention. Studies have shown that a diet high in fruits and vegetables is associated with a reduced risk of cancer. Apigenin is a non-toxic and non-mutagenic natural flavonoid. Our previous studies demonstrated the effects of apigenin on angiogenesis in CAM model is by two distintic parallel PI3K/AKT and jak/STAT pathways. Vascular endothelial growth factor (VEGF) is one of the most important of all known inducers of angiogenesis and in many cancers this protein is over-expressed. Studies confirm Apigenin efficient on reduction VEGF expression. In this study, as first time, effect of Apigenin is evaluated by oral administration on angiogenesis decreasing and VEGF expression levels were measured in creatures. After infection of mice's ovarian by A2780 cancer cell line, Apigenin (75/mg/kg/day) were fed once a day for 30 days to the animals and angiogenesis measured in vivo. Histological methods were used for counting blood vessels, ELISA analysis was used to measurement of VEGF protein in infected tissues. to confirm the effect of oral administration of Apigenin on VEGF expression, A2780 cells were infected by VEGF promoter reporter vectors then injected to the mice. Studies have shown that taking these orally, Apigenin reduced VEGF expression and ultimately leads to reduced angiogenesis. Citation Information: Mol Cancer Ther 2013;12(11 Suppl):C7. Citation Format: Majid Alipour. Inhibitory effects of flavonoid Apigenin by oral administration on angiogenesis of ovarian cancer through decrease VEGF expression in mouse model. [abstract]. In: Proceedings of the AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; 2013 Oct 19-23; Boston, MA. Philadelphia (PA): AACR; Mol Cancer Ther 2013;12(11 Suppl):Abstract nr C7.