This study evaluated how organic manures and mineral fertilizers affect winter wheat grain and straw yields and grain quality properties. The analysed period of the long-term fertilizer experiment was established in Čáslav, Czech Republic, in 1955 and covers the seasons 2011–2014. The fertilizer treatments were: control; farmyard manure (FYM); FYM + P; FYM + K; FYM + PK; FYM + N<sub>1</sub>; FYM + N<sub>2</sub>; FYM + N<sub>1</sub>PK; FYM + N<sub>2</sub>PK and FYM + N<sub>3</sub>PK. The highest grain yields were recorded in the FYM + P and FYM + N<sub>3</sub>PK treatments (8.9 t/ha). The highest straw yields were recorded in the FYM + N<sub>3</sub>PK treatment (6.52 t/ha). The lowest yields were provided in the unfertilized control and FYM treatments. Qualitative parameters were evaluated in the control, FYM and FYM + N<sub>3</sub>PK treatments between the years 2011 and 2013. The best quality of wheat grain was provided by the FYM + N<sub>3</sub>PK treatment. Combination of the farmyard manure with NPK is the best way to achieve high grain yields with good quality and leads to sustainable food production.