Effects Of Crude Aqueous Extract Research Articles

Multiple pharmacological targets, cytotoxicity, and phytochemical profile of Aphloia theiformis (Vahl.) Benn.

Aphloia theiformis (Vahl.) Benn. (AT) is traditionally used in Sub-Saharan African countries including Mauritius as a biomedicine for the management of several diseases. However, there is a dearth of experimental studies to validate these claims. We endeavoured to evaluate the inhibitory effects of crude aqueous extract as traditionally used together with the crude methanol extracts of AT leaves on urease, angiotensin (I) converting enzyme (ACE), acetylcholinesterase (AChE), cholesterol esterase (CEase), glycogen phosphorylase a (GPa), and glycation in vitro. The crude extract showing potent activity against the studied enzymes was further partitioned using different solvents of increasing polarity. The enzyme inhibitory and antiglycation activities of each fraction was assessed. Kinetic of inhibition of the active crude extract/fractions on the aforementioned enzymes was consequently determined using Lineweaver-Burk plots. An ultra-high performance liquid chromatography (UHPLC-UV/MS) system was used to establish the phytochemical profile of AT. The real time cell analysis system (iCELLigence™) was used to monitor any cellular cytotoxicity of AT. Crude methanolextract (CME) was a potent inhibitor of the studied enzymes, with IC50 ranging from 696.22 to 19.73μg/mL. CME (82.5%) significantly (p<0.05) inhibited glycation and was comparable to aminoguanidine (81.5%). Ethyl acetate and n-butanol fractions of CME showed non-competitive, competitive, and uncompetitive mode of inhibition against ACE, CEase, and AChE respectively. Mangiferin, a xanthone glucoside was present in CME, ethyl acetate, and n-butanol fractions. Active extract/fractions were found to be non-cytotoxic (IC50>20μg/mL) according to the U.S National Cancer Institute plant screening program. This study has established baseline data that tend to justify the traditional use of AT and open new avenues for future biomedicine development.

African Lettuce (Launaea taraxacifolia) Displays Possible Anticancer Effects and Herb-Drug Interaction Potential by CYP1A2, CYP2C9, and CYP2C19 Inhibition.

Medicinal plants are part of the healthcare systems worldwide, especially in low- and middle-income countries. African lettuce (Launaea taraxacifolia) is cultivated extensively in Africa, from Senegal in the west to Ethiopia and Tanzania in the east, and in Southern Africa. Potential anticancer effects of L. taraxacifolia have been suggested, but little is known about putative molecular mechanisms or potential for herb-drug interactions through inhibition or induction of drug-metabolizing enzymes. We investigated the effects of crude aqueous extracts of L. taraxacifolia on growth kinetics and cell cycle progression of the WHC01 esophageal cancer cells. Antiproliferative and apoptotic effects were evaluated using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and flow cytometry, while examining, in parallel, the genes regulating apoptosis and cell cycle in this cell culture model. In addition, we tested the inhibitory and enzyme kinetic effects of the aqueous L. taraxacifolia using recombinant human CYP450 isozyme model systems (CYP1A2, CYP2C9, and CYP2C19). L. taraxacifolia exhibited a significant growth inhibitory effect on the WHC01 cancer cells. Most cell cycle genes were downregulated. Cell cycle analysis showed a G0-G1 cell cycle arrest in WHC01 cells in the presence of L. taraxacifolia extract, accompanied by morphological changes. L. taraxacifolia extract treatment resulted in downregulation of expression levels of CYP1A2 (p < 0.0005) and CYP2C19 (p < 0.003) by 50-70%. L. taraxacifolia extract caused reversible and time-dependent inhibition of the recombinant CYP1A2, CYP2C9, and CYP2C19. This study provides new insights on possible anticancer effects of L. taraxacifolia, a widely used medicinal plant in parts of Africa and across the world especially by patients with cancer. Further mechanistic studies expanding on these observations would be timely and contribute to the field of global precision medicine that requires solid understanding of drug and herb molecular mechanisms of action and drug-herb interaction potentials, given the worldwide use of medicinal plants.

Effects of aqueous crude extract of Echeveria gibbiflora on mouse sperm function

ABSTRACTThe present study evaluates the possible antifertility effect of aqueous crude extract (OBACE) of Echeveria gibbiflora, a plant that belongs to the crassulaceae family, used in traditional Mexican medicine as a vaginal post coital rinse to prevent pregnancy and shown to have an immobilization/agglutination effect on sperm of different mammal species. We evaluated the effect of OBACE on functional parameters of mouse sperm, such as viability, capacitation, and acrosome reaction. In addition, due to the high concentrations of calcium bis-(hydrogen-1-malate) hexahydrate [Ca (C4H5O5)2•6H2O] present in this plant extract, we evaluated its effect on Ca2+ influx in mouse sperm under capacitating conditions. Moreover, we determined the acute toxicity of OBACE and its in vivo effect in mouse sperm motility administering a single daily dose of 50 and 100 mg/kg during seven days, intraperitoneally. The sperm viability was not affected by the presence of different concentrations of OBACE, however, the capacitation and acrosome reaction suffered a significant decrease in a concentration-dependent manner, coinciding with the reduction of Ca2+ influx. Furthermore, OBACE displayed an LD50 of 3,784.42 mg/kg and can be classified as a low toxic substance. Also, in vivo OBACE showed an inhibition of total and progressive motility on mouse sperm alongside a significant decrease of motility kinematic parameters and IVF rates. The results confirm the antifertility effect of this plant used in Mexican folk medicine. Further study on OBACE as a possible contraceptive treatment is warranted because of its activity and low in vivo toxicity.Abbreviations: ALH: lateral amplitude; AP: acid phosphatase; BCF: beat frequency; BSA: bovine serum albumine; CTC: chlortetracycline; FDA: fluorescein diacetate; Fura-2 AM: fura-2-acetoxymethyl ester; HIV: human immunodeficiency virus; IVF: in vitro fertilization; OBACE: aqueous crude extract of Echeveria gibbiflora; PI: propidum iodide; SN: supernatant; VAP: average path velocity; VCL: track speed; VSL: straight line velocity

Genotoxic, mutagenic and antigenotoxic effects of Cecropia pachystachya Trécul aqueous extract using in vivo and in vitro assays

Ethnopharmacological relevanceCecropia pachystachya is a medicinal plant native to South and Central Americas used to treat asthma and diabetes. Aim of the studyIn this study, we evaluated the genotoxic, mutagenic and antigenotoxic effects of crude aqueous extract of C. pachystachya (CAE-Cp) leaves. Material and methodsCAE-Cp was analyzed by the Folin–Ciocalteu method to determine total phenolic and tannin contents. High performance liquid chromatography (HPLC) was used to identify major compounds. Distinct tissues from female and male adult mice were treated with 500−2000mg/kg of CAE-Cp by gavage for the comet assay and micronucleus test analyses. In addition, peripheral blood slides of the group treated with 2000mg/kg CAE-Cp were analyzed 3, 6, and 24h after treatment and were exposed to hydrogen peroxide (ex vivo) to evaluate the genotoxic effect using the comet assay. The Salmonella/microsome assay was carried out against to TA100, TA98, TA97a, TA102, and TA1535 strains in presence and absence of the S9 mix. ResultsHPLC showed the presence of chlorogenic acid, isoorientin, orientin, and isovitexin as major compounds. Total phenolic and tannin contents were, respectively, 305.6±0.80 and 144.6±19.04mg of gallic acid equivalent/g of extract. Brain DNA damage was observed in all groups treated with CAE-Cp. The H2O2 challenge indicated genotoxic effect only 6h after the administration of the extract. No increase was detected in micronucleus frequency for any group treated with the extract. Mutagenic effects were detected by Salmonella/microsome assay only in TA102 strain without S9 mix at higher doses. ConclusionThe results obtained indicate that CAE-Cp was genotoxic to brain tissue. This result is supported by other papers, showing that compounds present in this extract can cross the blood-brain barrier and act on central nervous system.

تأثير المستخلصات المائية الخام لبعض أزهار النباتات الطبية على ورم إيرليش الصلب في الفئران

تم استخدام المستخلصات المائية الخام لزهور Cynara Cardunculus و Achillea millefolium و Calendula officinalis و Matricaria chamomilla من عائلة Compositeae في هذا العمل لدراسة كفاءتها في تخفيف ورم ehrlich الصلب في الفئران. المستخلص الأكثر فعالية هو C. officinalis الذي قلل من حجم الورم من 513.6 إلى 139.8 ملم بجرعة 200 مجم / كجم من وزن الجسم مقارنة بالدوكسوروبيسين الذي قلل حجم الورم إلى 98.2 ملم بتركيز 15 مجم / كجم بعد 21 يومًا ، يليه M. البابونج التي أظهرت متوسط ​​قيمة 168.7 ملم. بينما أظهرت أقل القيم مع مستخلص أزهار C. cardunculus و A. millefolium 198.7 و 223.5 ملم على التوالي. أيضا ، كانت العلاجات الأكثر فعالية هي دوكسوروبيسين بتركيز 15 مجم / كجم و C. officinalis ، يليه M. chamomilla و C. cardunculus و A. علامات مضادات الأكسدة (MDA ، TAC ، SOD) ، وظائف الكبد (ALT ، AST ، البروتينات الكلية ، الألبومين والجلوبيولين) ، وظائف الكلى (الكرياتينين واليوريا) ملف الدهون (الدهون الثلاثية ، الكوليسترول الكلي ، HDL ، LDL و vLDL) ومعلمات الدم ( HB و RBCs و Plt و WBCs) مقارنة بفئران الأورام الخاضعة للتحكم بجرعة 200 مجم / كجم من وزن الجسم لمدة 21 يومًا.

Cytotoxic Effect of Vincarosea Aqueous Crude Extraction Human Brain Carcinoma Cell Line (AMGM) In Vitro

The present study investigated the cytotoxic effects of aqueous crude extracts of Vincarosea leaves, flowers and seeds on Human brain carcinoma cell line (AMGA, Ahmed Majeed Glioblastoma Multiform ) in vitro, by using serial double dilution (concentration between 1.95-1000 µg/ml). The results showed that the cytotoxic effect of extracts was depended on type of parts of plant extracted, concentration and exposure time. The concentration 1000 µg/ml gave inhibition rate (IR), were (34, 49 and 64) % of leaves, flowers and seeds extracts respectively compared with control 100% after 24 hours from exposure time. However, low concentrations of aqueous extracts were found to induce the AMGA cells growth and proliferation (PR), it was 115% by treatment with aqueous extract offlowers extract in 1.95 µg/ml after 24 hours of exposed.

Effect of Crude Aqueous Extracts of Some Medicinal Plant Flowers on Solid Ehrlich Tumor in Mice

Effect of Crude Aqueous Extracts of Some Medicinal Plant Flowers on Solid Ehrlich Tumor in Mice

The effect of aqueous crude extract of ginger on the histology of corpus luteum and the concentration of hormones estrogen and progesterone in pregnant mice

This study was designed to investigate the effect of aqueous extract of ginger Zingiber officinale Roscoe on the histology of corpus luteum and the concentration of the hormones progesterone and estrogen during the first trimester of pregnancy (0 - 7) days from fertilization. 30 pregnant mice were divided into five experimental groups: control group (administrated with distilled water), and four groups treated at doses (284, 568, 1136,1420 mg / kg), orally administrated , daily with (0.1 ml). Microscopic examination results have shown histopathological changes in corpus luteum included: Pyknosis in some nuclei of granulosa cells, Karyorrhexis, Karyolysis in some granulosa cells, and necrosis in corpus luteum, with additional significant decrease in the average of diameters of corpus luteum at level (P

The effect of aqueous crude extract of ginger on the histology of corpus luteum and the concentration of hormones estrogen and progesterone in pregnant mice

This study was designed to investigate the effect of aqueous extract of ginger Zingiber officinale Roscoe on the histology of corpus luteum and the concentration of the hormones progesterone and estrogen during the first trimester of pregnancy (0 - 7) days from fertilization. 30 pregnant mice were divided into five experimental groups: control group (administrated with distilled water), and four groups treated at doses (284, 568, 1136,1420 mg / kg), orally administrated , daily with (0.1 ml). Microscopic examination results have shown histopathological changes in corpus luteum included: Pyknosis in some nuclei of granulosa cells, Karyorrhexis, Karyolysis in some granulosa cells, and necrosis in corpus luteum, with additional significant decrease in the average of diameters of corpus luteum at level (P

The Effect of Aqueous Crude Extract of Hypericum triquetrifolium on the (Number, Sex, Generation and their interactions) of Wild Fruit Flies

This study was designed to evaluate the effect of three concentrations of crude aqueous extracts of Hypericum triquetrifolium on (number, sex, generation and their combinations) of wild fruit flies. The concentrations (100, 50 and 25 µg/ml) were given to first generation parents in culture media and left for completing the life cycle under suitable temperature (25 Co ±2) in the modified-lightened incubator. Five replications were used for each treatment, and untreated flies were considered as control. The results were obtained in first and second generations successively. The results showed significant effect (P<0.05) of concentrations and concentration-generation interactions on the number of fruit flies and highly significant (P<0.01) of the impact of sex regarding the number of fruit flies.

Acute Toxicity and Histopathological Effects of Crude Aqueous Extract of Jatropha curcas Leaves in Mice

Acute Toxicity and Histopathological Effects of Crude Aqueous Extract of Jatropha curcas Leaves in Mice

Effect of Aqueous Crude Extract of Tinospora Crispa on Plasmodium Berghei Induced Liver Damage in Mice

Malaria is still a serious problem with increasing of mortality in children annually. One of major causes of death in malaria, organ damage especially liver, has been observed. Hence, we aimed to investigate hepatoprotective effect of traditional plant, Tinospora crispa during malaria infection using Plasmodium berghei infected mice as in vivo model. Aqueous crude extract of T. crispa was freshly prepared. For in vivo test, groups of ICR mice were intraperitoneally injected with 6×106 parasitized erythrocytes of P. berghei ANKA, and given with the extract at doses 10, 50, and 500 mg/kg twice a day for 4-consecutive days. Aspartate aminotransferase and alanine aminotransferase are measured for liver damage while albumin measurement is for liver function. The results showed that liver damage was observed during malaria infection as indicating by significantly (p<0.05) increase of AST and ALT, and markedly decrease of albumin. Interestingly, T. crispa extract exerted hepatoprotective effect during malaria infection. The highest hepatoprotective activity of the extract was shown at dose of 500 mg/kg. Additionally, there were no any toxics to liver function in normal mice treated with this extract. It can be concluded that aqueous crude extract of T. crispa exerts hepatoprotective effect during P. berghei infection.

Effects of Crude Aqueous Extract of Origanum vulgaris in Developing Ovary of Rabbits Following in Utero, Adolescent, and Postpubertal Exposure

We evaluated the impact on rabbits after having been treated with Origanum vulgaris (Lamiaceae) (OV) at a dose level known to adversely affect ovarian functions in rodents without causing systemic toxicity. The choice of rabbits has been guided by the fact that rabbits have a relatively long phase of reproductive development and hence simulation of reproductive development is better as opposed to dealing with rodents. The use of rabbits facilitates multiple evaluations of mating ability. An attempt has also been made at determining whether OV affected ovarian development and hence the use of animal model. Rabbits were exposed to 80 mg OV/kg/day in utero (gestation days [GD] 0 to23) or during adolescence (postnatal weeks [PNW] 4 by breast feeding and orally from 4w to 12 w), and the offspring were examined at the end of the 12 W period. Another group was treated after puberty (for 12 weeks) till age of 24 [PNW] of age and examined at the conclusion of exposure and follicles were categorized as primordial, primary, small preantral, large preantral or small antral follicles. The most pronounced reproductive effects were in female rabbits group which had been exposed from in utero till post-puberty period, in weights of ovaries (at 12 and 24 weeks, down 23%; p < 0.05). Serum Gonadotropin levels were down (at 24 weeks, 32%; p < 0.05); a slight increase in histological alterations of the ovaries (p < 0.05) at 24 weeks, of abnormal follicles;Â

Detrimental Effects of Mango Stem Bark on the Histology of the Prefrontal Cortex of Young Wistar Rats

The use of herbal preparations as a form of traditional medicine is common in this environment. The histological effects of crude aqueous extract of Mango stem bark was studied in pups of Wistar rats exposed to the extract during their intra-uterine life. Fifteen adult female rats were mated and grouped into three equal groups. Group A animals received water throughout gestation and served as Control, while Groups B and C were treated with 1 ml of the extract during the 2nd and 3rd trimesters respectively. Observation revealed lower birth weights in Group B, but higher birth weights in Group C, compared with the Control. Various degrees of architectural disruptions and degeneration were also noticed in the histological sections of the prefrontal cortex, which were nd more pronounced in the pups exposed to the extract during the 2nd trimester. The use of concoction of Mango stem bark should be discouraged, and more importantly during pregnancy, in view of its toxic effects on the developing brain, and as a potential predisposing factor to neurological dysfunctions.Keywords: mango stem bark, detrimental effect, prefrontal cortex, prenatal

AN ASSESSMENT ON THE PROTECTION OF GYMNEMA SYLVESTRE EXTRACTS AGAINST CASTOR OIL – INDUCED DIARRHOEA IN RATTUS NOVERGICUS

Diarrhoea is one of the main causes of death worldwide in children and adult alike. In view of this problem, the World Health Organization has encouraged studies for treatment and prevention of diarrhoea diseases depending on traditional medicinal practices. Anti-diarrhoeal effect of crude aqueous extracts of the stem, leaf and flower of Gymnema sylvestre was assessed in castor oil-induced diarrhoea at doses of 700, 1500 and 2000 mg/kg b.w, using Rattus novergicus. At these doses the extracts inhibited castor oil-induced diarrhoea in dose dependent fashion. There was significant (p<0.05) reduction in defecation severity when compared with the reference drug and control. The flower extract is more potent followed by the stem and the leaf. The result obtained in this study indicates that the aqueous extracts of Gymnema sylvestre may contain varied pharmacologically active ingredients with potent anti-diarrhoeal activity.

English

In vitro experiments were conducted to determine the anthelmintic effects of crude aqueous extracts of the leaves of Carissa spinarum and Azadrichta indica, fruits ofPhytolacca dodecandra and stem bark of Acacia tortilis on eggs and adults ofHaemonchus contortus using egg hatch assay and mortality of adult parasite. Extracts of the leaves of C. spinarum and A. indica inhibited hatching of egg at concentration less than or equal to 1 mg/ml. Low egg hatch inhibition were observed for extracts of A. tortilis(100%) and P. dodecandra (99.4%) at the maximum concentration tested (2 mg/ml). Of the plants tested, extracts of C. spinarum and A. indica showed very good activity against the adult worms of H. contortus, mortality raised to the levels of 96.8 and 93.9%, respectively, at concentration of 4 mg/ml. P. dodecandra and A. tortilis produced mortality of 68.1 and 53.03% of adult H. contortus at 4 mg/ml concentration, respectively. Albendazole killed the parasites in a dose dependant manner and all the worms were dead at a concentration of 0.5 mg/kg within 24 h. The overall findings of the current study indicated that most of the plants have potential anthelmintic effect warranting further in vitro and in vivo evaluation. Key words: Anthelmintic, Haemonchus contortus, in vitro experiment, plant extracts.

Antinociceptive Activity of Stephanolepis hispidus Skin Aqueous Extract Depends Partly on Opioid System Activation

Stephanolepis hispidus is one of the most common filefish species in Brazil. Its skin is traditionally used as a complementary treatment for inflammatory disorders. However, there are very few studies on chemical and pharmacological properties using the skin of this fish. This study was undertaken in order to investigate the effect of aqueous crude extract of S. hispidus skin (SAE) in different nociception models. Here, we report that intraperitoneal administration of SAE inhibited the abdominal constrictions induced by acetic acid in mice. In addition to the effect seen in the abdominal constriction model, SAE was also able to inhibit the hyperalgesia induced by carrageenan and prostaglandin E2 (PGE2) in mice. This potent antinociceptive effect was observed in the hot plate model too, but not in tail-flick test. Naloxone, an opioid receptor antagonist, was able to block the antinociceptive effect of SAE in the abdominal constriction and hot plate models. In addition, SAE did not present cytotoxic or genotoxic effect in human peripheral blood cells. Our results suggest that aqueous crude extract from S. hispidus skin has antinociceptive activity in close relationship with the partial activation of opioid receptors in the nervous system. Moreover, aqueous crude extract from S. hispidus skin does not present toxicity and is therefore endowed with the potential for pharmacological control of pain.

Crude aqueous extracts of Pluchea indica (L.) Less. inhibit proliferation and migration of cancer cells through induction of p53-dependent cell death

BackgroundPluchea indica (L.) Less. (Asteraceae) is a perennial shrub plant with anti-inflammatory and antioxidant medicinal properties. However, the anti-cancer properties of its aqueous extracts have not been studied. The aim of this study was to investigate the anti-proliferation, anti-migration, and pro-apoptotic properties of crude aqueous extracts of P. indica leaf and root on human malignant glioma cancer cells and human cervical cancer cells, and the underlying molecular mechanism.MethodsGBM8401 human glioma cells and HeLa cervical carcinoma cells were treated with various concentrations of crude aqueous extracts of P. indica leaf and root and cancer cell proliferation and viability were measured by cell growth curves, trypan blue exclusions, and the tetrazolium reduction assay. Effects of the crude aqueous extracts on focus formation, migration, and apoptosis of cancer cells were studied as well. The molecular mechanism that contributed to the anti-cancer activities of crude aqueous extracts of P. indica root was also examined using Western blotting analysis.ResultsCrude aqueous extracts of P. indica leaf and root suppressed proliferation, viability, and migration of GBM8401 and HeLa cells. Treatment with crude aqueous extracts of P. indica leaf and root for 48 hours resulted in a significant 75% and 70% inhibition on proliferation and viability of GBM8401 and HeLa cancer cells, respectively. Crude aqueous extracts of P. indica root inhibited focus formation and promoted apoptosis of HeLa cells. It was found that phosphorylated-p53 and p21 were induced in GBM8401 and HeLa cells treated with crude aqueous extracts of P. indica root. Expression of phosphorylated-AKT was decreased in HeLa cells treated with crude aqueous extracts of P. indica root.ConclusionThe in vitro anti-cancer effects of crude aqueous extracts of P. indica leaf and root indicate that it has sufficient potential to warrant further examination and development as a new anti-cancer agent.

The aqueous crude extract of Montanoa frutescens produces anxiolytic-like effects similarly to diazepam in Wistar rats: Involvement of GABAA receptor

Ethnopharmacological relevanceCihuapatli is the Nahuatl name assigned to some medicinal plants grouped in the genus Montanoa, where Montanoa frutescens (Family: Asteraceae, Tribe: Heliantheae) is included. The crude extract from these plants has been used for centuries in the Mexican traditional medicine as a remedy for reproductive impairments and mood disorders. Experimental studies have systematically corroborated the traditional use of cihuapatli on reproductive impairments and sexual motivation, however, the effect on mood and “nervous” disorders, remains to be explored. Materials and methodsThe anxiolytic-like effect of aqueous crude extract of M. frutescens (25, 50 and 75mg/kg) was investigated in male Wistar rats evaluated in the elevated plus-maze and compared with several doses of diazepam (1, 2 and 4mg/kg) as a reference anxiolytic drug. Picrotoxin (1mg/kg), a noncompetitive antagonist of the GABAA receptor, was used in experimental procedures to evaluate if this receptor could be involved in the anxiolytic-like effects produced by M. frutescens. To discard hypoactivity, hyperactivity, or no changes associated with treatments, which could interfere with the behavioral activity in the elevated plus-maze, rats were subjected to the open field test. ResultsM. frutescens at 50mg/kg showed anxiolytic-like activity similarly to 2mg/kg of diazepam, without disrupts in general motor activity. The anxiolytic-like effect of M. frutescens detected in the elevated plus-maze was blocked by picrotoxin, indicating that GABAA receptors are involved in the modulation of this effect. ConclusionsThe results corroborate the use of M. frutescens in folk Mexican ethnomedicine as a potential anxiolytic agent and suggest that this effect is mediated by the GABAA receptors. Additionally, some sedative effects with high doses of M. frutescens were detected in the present study.

Modulation of pancreatic MIN6 insulin secretion and proliferation and extrapancreatic glucose absorption with &amp;lt;i&amp;gt;Achillea santolina&amp;lt;/i&amp;gt;, &amp;lt;i&amp;gt;Eryngium creticum&amp;lt;/i&amp;gt; and &amp;lt;i&amp;gt;Pistacia atlantica&amp;lt;/i&amp;gt; extracts: &amp;lt;i&amp;gt;in vitro&amp;lt;/i&amp;gt; evaluation

Objective: The present in vitro studies aimed to investigate the pancreatic and extrapancreatic effects of crude aqueous extracts (AE) of Achillea santolina L, Eryngium creticum Lam, and Pistacia atlantica Desf utilized in Jordan diabetes ethnomedicine. Methods: Bioassays of β-cell proliferation and insulin secretion as well as glucose diffusion as possible modes of action were recruited. Results: Similar to L-alanine insulinotropic efficacy in MIN6 β-cell, glucose-stimulated Ca2+ regulated- insulin secretion was potentiated by AEs of E.creticum (0.01 mg/ml) and P.atlantica (0.01, 0.1 and 0.5 mg/ml). A.santolina AE, however, was found ineffective. Comparable to glucagon-like peptid-1-enhanced β-cell proliferation in 2-day treatment wells, a dose dependent augmentation of bromodeoxyuridine incorporation was obtained with the A.santolina AE (0.05-1 mg/ml), and E.creticum AE (0.1, 0.5 and 1 mg/ml). P.atlantica concentrations lacked pancreatic proliferative capacity. While A.santolina and E.creticum AEs proved inactive, P.atlantica inhibited dose dependently overnight glucose movement in vitro, as effectively as guar gum diffusional hindrance in a simple glucose dialysis model. Conclusion: Current findings signify the in vitro diverse therapeutic antidiabetes properties of the selected medicinal plants. Future directives may assess the use of A.santolina, E.creticum and P.atlantica as new potential sources of functional foods or nutraceuticals or active leads into diabetes type 2 pharmacotherapy.