Individual Selection Research Articles

A Multi-Strategy Differential Evolution Algorithm with Adaptive Similarity Selection Rule

The differential evolution (DE) algorithm is a simple and efficient population-based evolutionary algorithm. In DE, the mutation strategy and the control parameter play important roles in performance enhancement. However, single strategy and fixed parameter are not universally applicable to problems and evolution stages with diverse characteristics; besides, the weakness of the advanced DE optimization framework, called selective-candidate framework with similarity selection rule (SCSS), is found by focusing on its single strategy and fixed parameter greedy degree (GD) setting. To address these problems, we mainly combine the multiple candidates generation with multi-strategy (MCG-MS) and the adaptive similarity selection (ASS) rule. On the one hand, in MCG-MS, two symmetrical mutation strategies, “DE/current-to-pbest-w/1” and designed “DE/current-to-cbest-w/1”, are utilized to build the multi-strategy to produce two candidate individuals, which prevents the over-approximation of the candidate in SCSS. On the other hand, the ASS rule provides the individual selection mechanism for multi-strategy to determine the offspring from two candidates, where parameter GD is designed to increase linearly with evolution to maintain diversity at the early evolution stage and accelerate convergence at the later evolution stage. Based on the advanced algorithm jSO, replacing its offspring generation strategy with the combination of MCG-MS and ASS rule, this paper proposes multi-strategy differential evolution algorithm with adaptive similarity selection rule (MSDE-ASS). It combines the advantages of two symmetric strategies and has an efficient individual selection mechanism without parameter adjustment. MSDE-ASS is verified under the Congress on Evolutionary Computation (CEC) 2017 competition test suite on real-parameter single-objective numerical optimization, and the results indicate that, of the 174 cases in total, it wins in 81 cases and loses in 30 cases, and it has the smallest performance ranking value, of 3.05. Therefore, MSDE-ASS stands out compared to the other state-of-the-art DEs.

Genome-material costs and functional trade-offs in the autopolyploid Solidago gigantea (giant goldenrod) series.

Increased genome-material costs of N and P atoms inherent to organisms with larger genomes have been proposed to limit growth under nutrient scarcities and to promote growth under nutrient enrichments. Such responsiveness may reflect a nutrient-dependent diploid versus polyploid advantage that could have vast ecological and evolutionary implications, but direct evidence that material costs increase with ploidy level and/or influence cytotype-dependent growth, metabolic, and/or resource-use trade-offs is limited. We grew diploid, autotetraploid, and autohexaploid Solidago gigantea plants with one of four ambient or enriched N:P ratios and measured traits related to material costs, primary and secondary metabolism, and resource-use. Relative to diploids, polyploids invested more N and P into cells, and tetraploids grew more with N enrichments, suggesting that material costs increase with ploidy level. Polyploids also generally exhibited strategies that could minimize material-cost constraints over both long (reduced monoploid genome size) and short (more extreme transcriptome downsizing, reduced photosynthesis rates and terpene concentrations, enhanced N-use efficiencies) evolutionary time periods. Furthermore, polyploids had lower transpiration rates but higher water-use efficiencies than diploids, both of which were more pronounced under nutrient-limiting conditions. N and P material costs increase with ploidy level, but material-cost constraints might be lessened by resource allocation/investment mechanisms that can also alter ecological dynamics and selection. Our results enhance mechanistic understanding of how global increases in nutrients might provide a release from material-cost constraints in polyploids that could impact ploidy (or genome-size)-specific performances, cytogeographic patterning, and multispecies community structuring.

The role of thermal tolerance in determining elevational distributions of four arthropod taxa in mountain ranges of southern Asia.

Understanding the role of thermal tolerances in determining species distributions is important for assessing species responses to climate change. Two hypotheses linking physiology with species distributions have been put forward-the climatic variability hypothesis and the climatic extreme hypothesis. The climatic variability hypothesis predicts the selection of individuals with broad thermal tolerance in more variable climatic conditions and the climatic extreme hypothesis predicts the selection of individuals with extreme thermal tolerance values under extreme climatic conditions. However, no study has tested the predictions of these hypotheses simultaneously for several taxonomic groups along elevational gradients. Here, we related experimentally measured critical thermal maxima, critical thermal minima and thermal tolerance breadths for 15,187 individuals belonging to 116 species of ants, beetles, grasshoppers, and spiders from mountain ranges in central and northern Pakistan to the limits and breadths of their geographic and temperature range. Across all species and taxonomic groups, we found strong relationships between thermal traits and elevational distributions both in terms of geography and temperature. The relationships were robust when repeating the analyses for ants, grasshoppers, and spiders but not for beetles. These results indicate a strong role of physiology in determining elevational distributions of arthropods in Southern Asia. Overall, we found strong support for the climatic variability hypothesis and the climatic extreme hypothesis. A close association between species' distributional limits and their thermal tolerances suggest that in case of a failure to adapt or acclimate to novel climatic conditions, species may be under pressure to track their preferred climatic conditions, potentially facing serious consequences under current and future climate change.

Occupations, retirement, and the value of disability insurance

Occupations vary greatly in physical intensity, and there are, correspondingly, many differences in later-life work disability risk, retirement patterns, and applications for Social Security Disability Insurance (SSDI)—a national program that insures shocks to work productivity due to disability, with about nine million current beneficiaries in the U.S. In light of its widespread coverage and large differences in utilization across occupations, this paper aims to measure the value of the SSDI program across a broad population and the extent to which it influences the major choice of occupation. Using data from the Health and Retirement Study and O*NET, I estimate a life-cycle equilibrium model of occupational choice, work, savings, and disability at older ages, and find that incorporating occupations and preference heterogeneity is an integral part of understanding work and SSDI application behavior. While SSDI coverage is nearly universal and the premiums from workers are uniform, estimates suggest that the value of the program varies greatly, from being worth 2.1 to 14.5 percent of earned income—depending on preferences and choice of occupation—though for all groups it is welfare improving. I also find that SSDI plays an important role in the choice of occupation for older workers, providing an insurance value that results in over ten percent more people choosing physically intense, blue-collar occupations at older ages. This overall effect, however, masks the underlying selection of less risk-averse individuals into blue-collar jobs, which, if not accounted for, would lead to overstating the effects of the SSDI program on behavior. A counterfactual experiment aimed at eliminating the distortion created by uniform SSDI tax rates shows that—even without any additional employer accommodation of disabled workers—occupation-specific tax rates lead to fewer aggregate work-disabled years.

A Design and Analytical Strategy for Monitoring Disease Positivity and Biomarker Levels in Accessible Closed Populations.

In this paper, we advocate and expand upon a previously described monitoring strategy for efficient and robust estimation of disease prevalence and case numbers within closed and enumerated populations such as schools, workplaces, or retirement communities. The proposed design relies largely on voluntary testing, which is notoriously biased (e.g., in the case of coronavirus disease 2019) due to nonrepresentative sampling. The approach yields unbiased and comparatively precise estimates with no assumptions about factors underlying selection of individuals for voluntary testing, building on the strength of what can be a small random sampling component. This component enables the use of a recently proposed "anchor stream" estimator, a well-calibrated alternative to classical capture-recapture (CRC) estimators based on 2 data streams. We show that this estimator is equivalent to a direct standardization based on "capture," that is, selection (or not) by the voluntary testing program, made possible by means of a key parameter identified by design. This equivalency simultaneously allows for novel 2-stream CRC-like estimation of general mean values (e.g., means of continuous variables like antibody levels or biomarkers). For inference, we propose adaptations of Bayesian credible intervals when estimating case counts and bootstrapping when estimating means of continuous variables. We use simulations to demonstrate significant precision benefits relative to random sampling alone.

University peers and career prospects: The impact of university ties on early labor market outcomes

By using extensive Hungarian administrative data, this study aims to provide empirical evidence that former university ties strongly influence the labour market outcomes of individuals, even early in their careers. The estimates focus on the early career paths of graduates who obtained a master's degree between 2010 and 2017. As direct information on social contacts is not available in the dataset, we proxy university peers as students who started and finished the same university programmes (bachelor's or master's) in the same semester. Our results suggest that individuals are more likely to get hired by given firms if their former peers work there. The measured effects are considered significant and quite robust, even after controlling for the important sources of potential bias. Although we cannot present exact proof of the direct help of contacts, we provide suggestive evidence that seems to confirm the existence of such assistance. Our findings also revealed that the measured benefits are mainly attributable to connections from bachelor's studies. The effect of master's peers is mostly driven by the selection of individuals alongside prevalent study track-firm pathways. By comparing entries into new firms with and without peers, we also show that graduates with links have better labor market outcomes after hiring: they earn higher wages, obtain better and more prestigious positions, and stay longer at their new firm. The results draw attention to the importance of university peers in the labour market and contribute to the discussions about the determinants of early labour market success.

Widespread distribution of rodenticide resistance-conferring mutations in the Vkorc1 gene among house mouse populations in Portuguese Macaronesian islands and Iberian Atlantic areas

Growing evidence of widespread resistance to anticoagulant rodenticides (ARs) in house mice pose significant challenges to pest control efforts. First-generation ARs were introduced in the early 1950s but resistance to these emerged later that decade. Second-generation rodenticides were then developed, with resistance being reported in the late 1970s. Research has linked resistance to ARs with mutations in the Vkorc1 gene, leading to the use of more toxic and environmentally harmful compounds. In this study, 243 tail tips of house mice from mainland Portugal and Southern Spain, the Azores and Madeira archipelagos were analysed for all 3 exons of the Vkorc1 gene. Mutations L128S, Y139C, along with the so-called spretus genotype Vkorc1spr are considered responsible for reduced susceptibility of house mice to ARs. All these sequence variants were broadly detected throughout the sampling regions. Vkorc1spr was the most often recorded among mainland populations, whereas Y139C was nearly ubiquitous among the insular populations. In contrast, L128S was only detected in mainland Portugal and four islands of the Azores archipelago. All first generation ARs such as warfarin and coumatetralyl are deemed ineffective against all Vkorc1 variants identified in this study. Second-generation bromadiolone and difenacoum should also be discarded to control populations carrying Vkorc1spr, Y139C or L128S mutations. Inadequate use of ARs in regions where resistant animals have been found in large proportions will result in the spreading of rodenticide resistance among rodent populations through the positive selection of non-susceptible individuals. Consequently, ineffectiveness of rodent control will increase and potentiate environmental contamination, hazarding non-target wildlife through secondary poisoning. We highlight the need for Vkorc1 screening as a crucial tool in rodent management, aiding in the selection of the most appropriate control/eradication method in order to prevent misuse of these toxic biocides and the spread of rodenticide resistance among house mouse populations.

Individual variation in the habitat selection of upstream migrating fish near a barrier

BackgroundMigration is a vital element of the life cycle of many freshwater fish species but is increasingly hampered globally by riverine barriers. Fish passes are a common approach to enable migration past barriers but are often ineffective. More knowledge is required on fish behaviour as they approach barriers such as habitat preferences.MethodsWe evaluate the habitat selection of two upstream migrating fish species, barbel Barbus barbus and grayling Thymallus thymallus, at a hydropower plant in southern Germany, considering individual variation and population trends. Fish were tracked via fine-scale 2D acoustic telemetry in 2018 during their spawning migration. Step selection functions were used to evaluate selection of hydraulic parameters by the fish for a time step of 20 s. Exploratory models were built via model selection for each individual fish, to evaluate the extent of individual variation in model structure. A population model was developed for each species by averaging coefficients from individual models to describe general trends. The extent of individual variation was determined and confidence intervals for the population model coefficients were calculated.ResultsFish varied greatly in individual model structure though common terms were apparent in both species, such as depth, flow velocity, the angular difference between fish and velocity, and the logarithm of the step length. Final population models for barbel included several parameters describing habitat selection and displacement. Barbel selected for faster flows, deeper water, and higher spatial velocity gradients. In addition, they selected to move more with the flow than against. Interactions were also present between habitat parameters, suggesting selection is context dependent. Barbel movement speed also changed with depth, flow velocity and spatial velocity gradient. With grayling, terms often had contrasting effects among individuals and thus general trends could not be distinguished for most terms.ConclusionOur findings demonstrate habitat selection by upstream migrating fish approaching a fish pass and differences in individual selection which may have an impact on barrier management. Step selection functions are a promising approach and can provide useful insight into habitat selection and movement by migrating freshwater fish in an altered river system.

Long-term follow-up after endovascular treatment of aortic coarctation with bare and covered Cheatham platinum stents.

Endovascular treatment of aortic coarctation (CoA) in children and adults frequently requires stent implantation. The aim of this study was to analyze long-term results after CoA treatment with bare and covered Cheatham-PlatinumTM (CP) stents in our institution and to derive recommendations for the differential use of these stent types. In this retrospective single institution study, 212 patients received endovascular CoA treatment with bare (n = 71) and covered (n = 141) CP stents between September 1999 and July 2021, respectively. The indications for treatment were native CoA in 110/212 patients (51.9%) and re-coarctation after primary surgical or interventional treatment in 102/212 patients (48.1%). Median patient age at endovascular CoA treatment was 18.8 years [IQR 11.9; 35.8]. Long-term follow-up was available in 158/212 patients (74.5%) with a median follow-up of 7.3 years [IQR 4.3; 12.6]. Procedural success was achieved in 187/212 (88.2%) patients. Survival rate was 98.1% after 5, and 95.6% after 10 and 15 years, respectively. The probability of freedom from re-intervention was 93.0% after 5, 82.3% after 10 and 77.8% after 15 years, respectively. Freedom from re-interventions (44/158, 27.8%) did not differ between patients who received bare or covered CP stents (p = 0.715). Multivariable risk factor analysis identified previous CoA surgery (HR: 2.0, 95% confidence interval (CI): 1.1-3,9, p = 0.029), postdilatation (HR: 2,9, 95% CI: 1.1-6.3, p = 0.028) and age at intervention (HR: 0.96, 95% CI: 0.94-0.99, p = 0.002) as independent risk factors for re-intervention. Peri-procedural complications occurred in 15/212 (7.1%) patients (dissection/thrombosis of vascular access vessel: n = 9; bleeding: n = 1; stent dislocation: n = 2; aortic dissection/aortic wall rupture: n = 3). Long-term complications were observed in 36 patients and included stent fracture (n = 19), aneurysm formation (n = 14), endoleak (n = 1) and subclavian artery stenosis (n = 2). Peri-procedural and long-term complications did not differ between patients who received CoA treatment with bare or covered CP stents (all p > 0.05). Endovascular treatment of CoA using bare or covered CP stents can be performed safely and effectively with excellent long-term results. Survival, re-intervention and complication rate did not significantly differ between both stent types. However, individual stent selection is advisable with regard to CoA morphology and severity as well as patient age.

Reproductive roles as likely drivers of sexual dimorphism in New Zealand’s endangered mountain parrot, the Kea

Abstract New Zealand’s endangered mountain parrot, the Kea (Nestor notabilis), exhibits moderate male-biased sexual size dimorphism in linear body measurements (~5%) and a pronounced dimorphism in bill size (12–14%). Using stable isotope analyses of carbon and nitrogen in Kea feathers and blood sampled from a significant portion (~10%) of the extant population, we determined that Kea bill dimorphism may be an ecologically selected trait that enhances male Kea’s ability to forage at a higher trophic level in order to provision females and offspring during nesting. Sexual dimorphism can arise through sexual selection, ecological drivers, or a combination of both. Ecological selection is associated with foraging niche divergence between the sexes to reduce inter-sexual competition or due to differing dietary needs associated with reproductive role. Despite the widespread occurrence of sexual dimorphism throughout the animal kingdom, empirical evidence for ecological causation is rare. We conducted the first molecular confirmation of sexual size dimorphism in Kea. We then employed Bayesian mixing models to explore potential correlations between diet and bill size to determine whether the dimorphism is linked to diet partitioning throughout all age classes (fledgling, juvenile, subadult, and adult). Female Kea foraged at a consistent, relatively low, trophic level throughout their lifetime, whereas male trophic level increased with age to a maximum at subadult stage, prior to breeding for the first time—a time in which males may have been actively learning extractive foraging techniques associated with a high protein diet. Adult males foraged at a high trophic level relative to all groups except subadult males. As males provision females on the nest, which in turn provision young, these results highlight that the evolution of morphology and reproductive output may be linked in circuitous ways.

Theory of (1+1) ES on SPHERE Revisited

The theory of evolutionary algorithms on continuous space gravitates around the evolution strategy with one individual, adaptive mutation and elitist selection, optimizing the symmetric, quadratic SPHERE function. The classic, normal mutation theory consists of three main building-blocks: i) a two-term formula for the local (constant mutation) expected progress; ii) an exponential formula for the global behavior of the (adaptive mutation) algorithm; iii) linear convergence time with respect to both space dimension n and (logarithm of) initial distance to optimum. We show that the three main results still hold if we replace the normal mutation with uniform inside the sphere and also with the sum of two uniforms. That makes the case for an important conclusion: the linear convergence time of the algorithm is not a consequence of the normal mutation, but of the elitist selection and 1/5 success rule. A simplified version of the 1/5-rule allows also for an intuitive representation of the algorithm, as a sequence of constant-mutation, independent and identical (expected) length cycles.

Genetic Polymorphisms Associated with the Efficiency of Weight Loss: A Systematic Review

Interindividual differences are associated not only with body weight but also determine the body’s response to diet and physical activity. This systematic review aimed to identify the genetic markers associated with diet-induced and exercise-induced weight loss. The eligible intervention studies for this systematic review contained all the necessary parameters for genetic polymorphism, diet, exercise, and changes in anthropometric or body composition parameters. In total, 91 articles met the criteria and were included in this systematic review. The vast majority of studies (n = 88) are used of candidate gene approach, and only three studies were performed using hypothesis-free approach (GWAS). A total of 98 genetic variants were found, of which 72 markers are associated with the efficiency of diet therapy, and 26 – with individual response to physical activity. It should be noted that the significance of markers was replicated for only 10 of 98 genetic variants. In the near future, we should expect progress in this area, which will result in the development of a method for individual selection for each patient of the type of diet and exercise for the prevention and treatment of obesity.

Meta-Analysis of Influence of Diversity of Parental Forms on Heterosis and Specific Combining Ability of Their Hybrids

An important stage in any breeding activity is selection of suitable individuals for further breeding. Thus, the main goal of breeders becomes such a selection of parental forms that leads to the consolidation and maximization of the value of traits of significant utility and economic importance. Heterosis and specific combining ability are very important parameters in plant and animal breeding. The ability to predict their value and relevance could significantly shorten the breeding process. One way to predict the effects of heterosis and specific combining ability is to select parental forms for crosses. This selection can be made on the basis of variation in parental forms. An analysis was made of publicly available data that contain information about the effects of heterosis, the effects of specific combining ability, and phenotypic and genetic diversity of parental forms. Preliminary studies show that the best approach for obtaining favorable hybrids would be selection of parental forms that are very genetically diverse while being phenotypically equal.

Retrospective analysis of spring barley cultivars developed by Omsk breeders (1936–2021)

Plant breeding is an endless conveyor belt: cultivars developed earlier are included in hybridization plans and become the sources for new ones. The main tasks in barley improvement through breeding include raising the productivity of cultivars, improving their quality, and strengthening disease resistance. Correct selection, use, and studying of the source material – that is the key to successful breeding efforts. The first and most important step in constructing a cultivar is the process of hybridization. A valuable source of aid, and often the basis for newly developed cultivars, is the global collection of VIR, whose importance can hardly be overestimated. From 1936 to 2021, 27 barley cultivars were released: they are widely cultivated in Russia and Kazakhstan. Of these, 19 cultivars were obtained by pair hybridization; 4 cultivars by the complex stepwise hybridization technique; 4 cultivars through individual selection using the population approach. Despite the high importance of germplasm collection materials, 21 cultivars were produced by hybridization using a genotype selected at Omsk as one of the parental forms: in 16 cultivars, a local genotype was used as a maternal parent; in 12, as a paternal one; and in 8 cultivars, both parent forms were bred at Omsk Agricultural Scientific Center. Our retrospective analysis of a number of Omsk spring barley cultivars, on the one hand, confirms the earlier conclusions of Siberian scientists about a limited number of base crop cultivars, and on the other, attests to very rich pedigrees of spring barley cultivars in terms of the number of parent forms from the VIR global collection. Siberian barley ecotypes, characterized by increased adaptability to local harsh climate factors, should be further preserved in the national crop germplasm repository at VIR.

Functional constipation in preschool and school age children: ways to solve the problem

Functional constipation is a significant medical problem in pediatrics. Constipation often causes discomfort in children of any age and, in the absence of timely and effective treatment, can lead to chronic intoxication and the development of various complications, ultimately affecting the patient's quality of life. The article presents the definition of functional constipation in children over 4 years of age in accordance with the Rome IV Criteria (2016), etiology, pathophysiological mechanisms, clinic, diagnostic criteria and differential diagnosis of functional and organic constipation in preschool and school age children, possible complications. In accordance with the draft Consensus of the Society of Pediatric Gastroenterologists (2013), for a more objective assessment of the nature of the stool, the Bristol scale of stool forms is given. The physiology of the colon and the role of motor disorders in the occurrence of functional constipation are described. The physiology of the colon and the role of motor disorders in the occurrence of functional constipation are described. In accordance with the recommendations of the National School of Gastroenterology, the National Institute of Health and Clinical Excellence (NICE) in the UK, ESPGHAN and NASPGHAN, the basic principles of constipation therapy in preschool and school age children are given. The main groups of drugs that are used in the treatment of constipation in children of this age are described: stimulating and osmotic laxatives, prokinetics. Numerous foreign studies and clinical recommendations indicate that stimulants are effective and safe for the treatment of functional constipation, including in children's practice and are most in demand. The analysis of the use of one of the main drugs of the group of stimulating laxatives - sodium picosulfate in numerous international and Russian clinical studies is presented. The effectiveness and favorable safety profile of sodium picosulfate, the possibility of individual dose selection in connection with the liquid form of release are shown. A clinical example of the treatment of functional constipation in a 6-year-old child is given.

Round-Window Vibroplasty: Systematic Review and Meta-Analysis of Audiological Effectiveness With Different Round-Window Coupling Techniques.

Active middle ear implants can be directly coupled to the round-window (RW) membrane via RW Vibroplasty. The objectives of this systematic review were to summarize data on different RW coupling techniques and to investigate their effect on audiological and safety outcomes using meta-analyses. PubMed (MEDLINE), Cochrane Library, and Embase (DIMDI). All publications reporting on audiological outcomes in human patients after RW Vibroplasty were included. Two independent reviewers carried out screening and data extraction. Meta-analyses and meta-regression were used to evaluate the potential effects of surgical and demographic parameters on primary audiological outcomes. Adverse events were extracted and tabulated for qualitative analysis. Fourteen different combinations of surgical coupling parameters were identified in 61 included publications. Overall, data from 23 publications could be used for meta-analyses. Significantly better aided sound-field thresholds were reported for RW Vibroplasty performed without a dedicated coupler and using fascia as interponate, compared with RW Vibroplasty with an RW coupler or without an interponate. These effects were not found in other outcomes (i.e., functional gain, word recognition score). Reporting of adverse events was relatively heterogeneous, with 32 explicit mentions of revision surgery after the loss of coupling efficacy. There are numerous publications investigating the effectiveness of coupling active middle ear implants to the RW membrane. However, studies are typically undersampled and of low evidence level. Using meta-analyses, weighted means, and qualitative analyses enabled a summary of the existing literature on audiological and safety outcomes after RW Vibroplasty. Individual selection of the most appropriate coupling modality with standardized intraoperative measurement and careful patient follow-up may be considered key factors for achieving effective RW coupling.

Individual Feature Selection of Rolling Bearing Impedance Signals for Early Failure Detection

Condition monitoring of technical systems has increasing importance for the reduction of downtimes based on unplanned breakdowns. Rolling bearings are a central component of machines because they often support energy-transmitting elements like shafts and spur gears. Bearing damages lead to a high number of machine breakdowns; thus, observing these has the potential to reduce unplanned downtimes. The observation of bearings is challenging since their behavior in operation cannot be investigated directly. A common solution for this task is the measurement of vibration or component temperature, which is able to show an already occurred bearing damage. Measuring the electrical bearing impedance in situ has the ability to gather information about bearing revolution speed and bearing loads. Additionally, measuring the impedance allows for the detection and localization of damages in the bearing, as early research has shown. In this paper, the impedance signal of five fatigue tests is investigated using individual feature selection. Additionally, the feature behavior is analyzed and explained. It is shown that the three different bearing operational time phases can be distinguished via the analysis of impedance signal features. Furthermore, some of the features show a significant change in behavior prior to the occurrence of initial damages before the vibration signals of the test rig vary from a normal state.

Sources of Economically Valuable Features for Selection of Grass Pea (Lathyrus sativus L.) in Conditions of Absheron Peninsula

Background: In a world facing climate change and associated environmental stresses that hamper agricultural productivity and food security, the requirement for more sustainable agriculture is on the rise. In this regard, there is a need to expand the area of cultivation of drought-resistant leguminous crops. The purpose of the research is to study the grass pea collection, to identify forms with economically valuable traits and to create on their basis a new highly productive, technologically advanced, valuable with its quality grass pea grain for a selection in the conditions of the Republic of Azerbaijan. Methods: The studies were conducted in 2017-2020 at the Institute of Genetic Resources (IGR) of the National Academy of Sciences (NAS) of Azerbaijan. 75 samples were used as research material:25 of them were local forms and 50 were samples obtained from ICARDA. The productivity and structure of the harvest have been analyzed. Result: As a result of research, the most high-yielding and high-quality samples GP-58, GP-59, GP-65, GP-71, GP-73, İFLA-2973, İFLA-240, İFLA-479, İFLA-1795, İFLA-242, GP-56, GP-87. were revealed in the studied grass pea samples. During the implementation of the breeding program using the results, we have developed a new early ripe, drought-resistant, heat-resistant, disease-resistant and high-yielding variety Zirve by the method of repeated individual selection from the local forms. These studies are an important initial study for the subsequent production of new varieties. We hope that this will lead to an increase in the acreage of grass pea in the Republic of Azerbaijan.

Modern opportunities for assessing the left ventricle remodeling

Aim: To compare the results of distributing left ventricular (LV) remodeling patterns according to two classifications and identify their advantages and disadvantages. Materials and methods. The study involved 2019 patients, aged from 18 to 94 years with a mean of 57.6 ± 16.4 years, 51 % males, apparently healthy and with cardiovascular and respiratory system pathology. Echocardiography was performed using an Esaote MyLab Seven device (Italy) according to generally accepted standards. The distribution of patients according to four classic LV remodeling patterns and according to the classification proposed by W. Gaasch and M. Zile was studied. Statistical analysis was performed using Statistica for Windows 13.0 (StatSoft Inc., USA; license No. JPZ804I382130ARCN10-J). Qualitative variables were given as absolute and relative frequency (n (%)). Results. The distribution of patients according to 4 classic LV remodeling patterns was as follows: 53.0 % had normal LV geometry; 10.2 % – concentric remodeling; 15.6 % – concentric hypertrophy; 21.2 % – eccentric hypertrophy. At the same time, in the group of normal LV geometry, 25.2 % of people had LV dilatation. When distributing the patients by remodeling patterns according to the W. Gaasch and M. Zile classification, the following data were obtained: 26.9 % of people had normal LV geometry, concentric remodeling – 10.2 %, concentric hypertrophy – 12.4 %, mixed hypertrophy – 3.1 %, physiological and dilated hypertrophy – 11.8 %, eccentric hypertrophy – 5.5 %, eccentric remodeling – 11.4 %. In addition, 3 more groups of patients were formed, who did not have a terminological definition based on the W. Gaasch and M. Zile classification and, in total, accounted for 18.6 % of the examined. Conclusions. The missing options of dilated left ventricle should be recognized as a limitation of the classic classifications of left ventricular remodeling patterns. The imposition of additional criteria covering the cavity dilatation in determining the left ventricular remodeling patterns improves the assessment of the patient’s prognosis and contributes to a more individual selection of pathogenetic therapy.

MACS-DGC sperm preparation method resulted in high-quality sperm, top-quality embryo, and higher blastocyst rate in male factor infertile couples with high DNA fragmented sperm

Conventional sperm selection based on motility and morphology fails to provide detailed information on sperm functional and molecular status. Magnetic-activated cell sorting (MACS) protocol aims to optimize this process by selecting apoptotic sperm cells. Phospholipase C zeta-1 (PLCz1) is a physiological stimulus for oocyte activation and early embryonic development. The purpose of this study was to examine seminal parameters, DNA fragmentation index (DFI), and PLCz1 expression levels in MACS-DGC sorted specimens (DFI > 30%) and assess early development in resulting embryos. Semen specimens from 60 patients diagnosed with male factor infertility were collected and processed by either density gradient centrifugation (DGC) or MACS-DGC protocols. Pre and post-preparation analysis was performed. PLCz1 expression was assessed using the RT-PCR method. Retrieved eggs from their partners were divided into two groups in which they were injected with different sorted sperm. The fertilization rate and embryonic development were evaluated. While sperm’s progressive motility and morphology significantly improved, there was a substantial decline in DFI following MACS-DGC. Fertilization rates were almost the same between the groups, and the latter resulted in remarkably more top-quality embryos and more blastocysts. PLCz1 expression was considerably higher in the MACS-DGC group. By eliminating apoptotic cells, the MACS-DGC technique could sort highly PLCz1-expressed sperm, optimize sperm selection in individuals with elevated DFI, development of resulting embryos.