Exposure to heavy metals has been associated with the generation of reactive oxygen species (ROS) among exposed individuals in occupational and environmental settings. Dust is considered a significant contributor to airborne metal exposure, and previous data suggest that their levels in dust may vary based on its particle sizes. However, no biomonitoring study has been reported so far to address the metal-induced oxidative stress using different dust fractions, particularly in occupational settings. We designed a systematic cross-sectional study involving 110 chromite mine workers stratified into loaders (n = 28), extractors (n = 47) and operators (n = 35), and controls (n = 30) to find out the association between dust-bound metal exposure and oxidative stress using urinary creatinine-adjusted metal level as a biomarker of metal exposure. Results suggested elevated urinary levels of Cr 51.34 ± 8.6 along with Pb 34.29 ± 4.39, Cd 21.1 ± 2.6, and Ni 18.98 ± 3.01µg/g creatinine in exposed (extractor group) workers. Correlating metal levels with oxidative stress revealed elevated malondialdehyde (MDA) levels of 62.28 ± 5.52nM/dl among the extractors showing high levels of lipid peroxidation. Furthermore, blood superoxide dismutase (SOD) was also found significantly correlated (P = 0.000) with urinary toxic metal levels among exposed workers. We report the association between metal exposure and oxidative stress in exposed mining workers that may give rise to workers' susceptibility towards genetic and non-genetic health implications. The current study emphasized on the need for exposure control measures in the chromite ore mining activity areas.
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