CRISPR-integrated nanoconfined interparticle catalytic hairpin assembly for enhanced dual-mode SARS-CoV-2 detection in wastewater.

DM-FDA: a dual-modal feature detection algorithm for microscopic impurities

Electrochemical-colorimetric dual-mode detection of zearalenone based on restriction endonuclease-driven DNA walker system.

N, P and S co-doped red fluorescent carbon dots sensing for nitrite by fluorescent/colorimetric/smart phone/test strip methods through diazo coupling reaction in food samples.

Fluorescence/smartphone dual-mode detection of captopril based on hydroxytyrosol-naphthoresorcin fluorogenic reaction and Eu MOF

Dual-mode fluorescence/colorimetric aptasensor based on Zn,Ce-MOF nanocomposite for sensitive detection of isocarbophos.

A dual-reporter HCoV-OC43 for coronavirus biology and countermeasure development.

A dual-mode microfluidic chip embedded with photoelectrochemical sensing and fluorescence imaging for phenotypic analysis of tumor cells.

Precisely engineered dual-antenna europium MOF with intrinsic dual-state luminescence for sensitive dexamethasone sensing.

Selective molecular interplay in electrochemical sensing: DNA-templated Hg(II) capture and enzyme-driven SeO42- reduction for mercury removal.

Phage-induced inner filter effect powers sensitive dual-mode detection of E. coli O157:H7

AuNP aggregation-triggered conformational changes in hairpin probes for dual-mode colorimetric and SERS detection of TTX.

DNAzyme walker-triggered HCR dual amplification mediated by MOF-derived bimetallic Fe-Ni-doped porous carbon nanozyme for homogeneous dual-mode aptasensor detection of profenofos.

Amultifunctional polydopamine-gold (PDA@Au) core-shell nanoprobe has been synthesizedthat simultaneously enables surface-enhanced Raman scattering (SERS), photothermal conversion, and visual signal output within a single structure. Based on these features, we developed a dual-modal LFA platform for H1N1 antigens detection through two complementary readout modes. The SERS and photothermal modes achieved detection limits of 1.34pg/mL and 1.03pg/mL, corresponding to 746-fold and 971-fold improvements compared to commercial Au-based LFAs. The platform also exhibited excellent specificity against other common respiratory viruses and high batch-to-batch reproducibility across detection modes. Clinical validation on 90 throat swab specimens showed strong agreement with RT-PCR results. The PDA@Au-LFA demonstrated sensitivities of 98.5% (SERS), 97.0% (photothermal), and 93.9% (visual), along with consistently high specificity (95.8%). These results highlight the PDA@Au-based LFA as a high-performance diagnostic platform, featuring sensitive, specific, and reproducible multimodal detection enabled by the integration of a novel signal transduction material.

Cysteine (Cys) serves as a fundamental building block for protein synthesis and drug development. Due to that l-Cys plays a crucial role in neurological function and cellular detoxification, whereas d-Cys exhibits notable cytotoxicity and health risks, and the rapid and selective assay of d-Cys in l-Cys-related pharmaceuticals is critically important. Here, a multifunctional chiral recognition probe was synthesized to achieve photoelectrochemical (PEC) and red-green-blue (RGB) colorimetric dual-mode selective detection of d-Cys in the presence of l-Cys. Through thiol-ene click chemistry, a d-Cys modified covalent organic framework (d-COF) was synthesized with abundant chiral recognition sites. Driven by homochiral interactions, d-COF exhibits a significantly stronger adsorption affinity for d-Cys than l-Cys. The adsorbed Cys acts as an electron donor to enhance the photocurrent of d-COF, yielding a high chiral recognition efficiency of ΔID/ΔIL = 12.0 in PEC mode. Furthermore, the strongly adsorbed d-Cys protonates the imine bonds in d-COF via its carboxyl and thiol groups, inducing a distinct color change from dark yellow to red, whereas l-Cys adsorption causes negligible color change due to the weak interaction between d-COF and l-Cys. Thus, based on the obvious difference in photocurrent and color changes of d-COF adsorbed with d-Cys and l-Cys, selective detection of d-Cys through PEC-RGB dual-mode sensing platform is achieved in the presence of l-Cys. This work demonstrates a PEC-RGB dual-mode sensing platform for selective detection of d-Cys and a generalizable strategy that can be extended to other chiral substance assays by changing the chiral selector, showing promising potential in chiral drug identification and biomedical detection.

Dual-Mode Histamine Detection Using Nitrogen and Iron Co-Doped Carbon Dots: A Novel Nanozyme-Based Sensing Platform for Food Safety Applications

Plasmonic core-shell nanoparticles regulated FRET process for ultrasensitive dual mode detection of riboflavin.

Multi-functional copper-manganese Nanozyme-Driven integrated platform for Fluorescence/Colorimetric Dual-Mode Detection, catalytic degradation of oxytetracycline and antibacterial disinfection

Mixed-type micro-defect detection in semiconductor Wafers: A dual-modal feature real-time detection approach via optical topography and lightweight classification network

Integrated portable dual-mode multimorphic ferromagnetic metal defect detection device based on diamond NV centers