Dual Imprinting Research Articles

Dual-imprinted organic/inorganic nanocomposite membranes with highly selective polydopamine-intimated nanostructures for pharmaceutically active compound separation

Here, the graphene oxide (GO)/SiO2-loaded dual-imprinted membranes (GS-DIMs) were constructed based on the self-polymerization imprinting technique of dopamine, in which a twice polydopamine (PDA)-based imprinting strategy had been successfully developed to obtain the three-dimensional nanocomposite membrane-based separation system. Meanwhile, the pollution-intensive antibiotics of tetracycline (TC) was used as template molecule throughout the GS-DIMs synthesis, and the dopamine molecules were simultaneously used as functional monomer and cross-linking agent during the twice polydopamine (PDA)-based imprinting processes. Therefore, dual-TC-imprinted sites had been prepared based on the as-designed dual imprinting processes, the as-prepared GS-DIMs-based separation system with dual-TC-imprinted structures could not only allow for the largely enhanced rebinding result of 65.61 mg/g and faster adsorption equilibrium rate within 20 min, but also facilitate the permselectivity performance from TC-based complex separation system and mimetic water sample. Importantly, we demonstrated the applications and effects of the dual-imprinted membrane-based separation materials to selective rebinding and separation of TC from complex solution systems and mimetic water samples. The as-obtained permselectivity factors (β) around 4.0 strongly illustrated the efficiently selective separation ability and high-intensitive recognizability of TC than any other non-template molecules based on our GS-DIMs-based separation system. Overall, the as-designed GS-DIMs had great potential for selective separation applications and provided critical comparisons based on the as-achieved excellent rebinding and permselectivity performance, which encompassed innovative GO/SiO2-loaded nanocomposite and PDA-based dual-TC-imprinted system.

Simultaneous Detection of Two Tumor Marker Proteins Using Dual-Colored Signaling Molecularly Imprinted Polymers Prepared via Multi-Step Post-Imprinting Modifications

Abstract We report simultaneous detection of tumor marker proteins using a molecularly imprinted polymer-based fluorescence sensing system, in which prostate-specific antigen (PSA) recognition cavity, labeled with Alexa Fluor 594, and α-fetoprotein (AFP) recognition cavity, labeled with Alexa Fluor 647, exist together in the polymer matrix. The individually fluorescent-labeled PSA- and AFP-imprinted polymer was prepared by a dual imprinting method, followed by multi-step post-imprinting modifications (PIM). A polymerizable group, conjugated with PSA or AFP via a disulfide bond, was prepared and immobilized on a phenylboronic acid moiety-introduced substrate by the formation of cyclic diester between phenylboronic acid and glycans on proteins. The polymer matrix was prepared using surface-initiated atom transfer radical polymerization. After the reduction of the disulfide bond and hydrolysis of the cyclic diester, PSA- and AFP-imprinted nano-cavities were generated simultaneously. In multi-step PIM, thiol-reactive fluorescent dyes were introduced via a dynamic protection procedure using the target protein, which yielded dual fluorescence-labeled imprinted nano-cavities. Fluorescence signaling abilities were assessed, and each AFP and PSA-imprinted nano-cavity was confirmed to transduce the protein binding events into specific fluorescence signals, with lower values of limit of detection (<2.0 ng/mL). Therefore, the proposed methodology could be a novel platform for the simultaneous detection of multiple proteins.

Does tissue imprinting restrict macrophage plasticity?

Macrophages have long been considered as particularly plastic cells. However, recent work combining fate mapping, single-cell transcriptomics and epigenetics has undermined the macrophage plasticity dogma. Here, we discuss recent studies that have carefully dissected the response of individual macrophage subsets to pulmonary insults and call for an adjustment of the macrophage plasticity concept. We hypothesize that prolonged tissue residency shuts down much of the plasticity of macrophages and propose that the restricted plasticity of resident macrophages has been favored by evolution to safeguard tissue homeostasis. Recruited monocytes are more plastic and their differentiation into resident macrophages during inflammation can result in a dual imprinting from both the ongoing inflammation and the macrophage niche. This results in inflammation-imprinted resident macrophages, and we speculate that rewired niche circuits could maintain this inflammatory state. We believe that this revisited plasticity model offers opportunities to reset the macrophage pool after a severe inflammatory episode.

Fabrication of CoNPs-embedded porous carbon composites based on morphochemical imprinting strategy for detection of H2O2 released from living cells

Rational construction of biosensor to determine reactive oxygen species (ROS) generated from living cells is one of the great challenges in physiological and pathological fields. Herein, for the first time, a novel Co nanoparticles (CoNPs) embedded walnut-like N-doped porous carbon microsphere (Co@NCS) can be synthesized using dual imprinting strategy by pyrolysis and acid leaching. The distinct morphology and chemical characteristic of melamine cyanurate (MC) aggregates can be imprinted in the carbon source derived from glucose during the pyrolysis process. According to our research, with synergistic effect of CoNPs and NCS, the Co@NCS shows an excellent electrochemical activity toward the reduction of hydrogen peroxide (H2O2). The Co@NCS modified electrode exhibits a wider linear detection response with the range from 0.5 μM to 7500 μM and a low detection limit of 88 nM (S/N = 3). Moreover, with desirable selectivity, decent reproducibility and satisfied anti-interference performance, the fabricated biosensor can be applied to determine H2O2 in human serum samples and that released from living cell, indicating the potential applications in the fields of pathological and physiological sensing.

Ion/molecule imprinted polymers with double binding sites via twice imprinting strategy for selective and simultaneous removal of λ-cyhalothrin and Cu(II)

Abstract Herein we reported a facile method to prepare porous ion/molecule imprinted polymers (I-MIPs) via twice imprinting strategy for the simultaneous uptake of copper ions (Cu(II)) and λ-cyhalothrin (LC). Firstly, N -(β-aminoethyl)-γ-aminopropytrimethoxysilane (DAMO) was grafted on silica particles surface to synthesis Cu(II)-imprinted polymers to introduce iron imprinted sites. They were then utilized as stabilizers to prepare a stable W/O Pickering HIPEs template (LC as the imprinting molecule), which was then polymerized with dual imprinting sites including Cu(II) and LC. The adsorption equilibrium, kinetic results and selective recognition experiments were exhibited their prospected dual selectivity and simultaneous removal of LC and Cu(II).