Downy Mildew Research Articles

Genome-Wide Reidentification and Expression Analysis of MADS-Box Gene Family in Cucumber

MADS-box transcription factors play a crucial role in plant growth and development. Although previous genome-wide analyses have investigated the MADS-box family in cucumber, this study provides the first comprehensive reannotation of the MADS-box gene family in Cucumis sativus using updated Cucurbitaceae genome data, offering novel insights into the gene family’s evolution and functional diversity. The results show that a total of 48 CsMADS-box genes were identified in the V3 version of cucumber, while 3 of the 43 genes identified in the V1 version were duplicated. The V1 version actually has only 40 genes. Additionally, we analyzed the variability in protein sequences and found that the amino acid sequences of 14 genes showed no differences between the two versions of the database, while the amino acid sequences of 29 genes exhibited significant differences. The further analysis of conserved motifs revealed that although the amino acid lengths of 15 genes had changed, their conserved motifs remained unchanged; however, the conserved motifs of 12 genes had altered. Furthermore we found that motif1 and motif2 were present in most proteins, indicating that they are highly conserved. Gene structure analysis revealed that most type I (Mα, Mβ) MADS-box genes lack introns, whereas type II (MIKC) genes exhibit a similar structure with a higher number of introns. Chromosomal localization analysis indicated that CsMADS-box genes are unevenly distributed across the seven chromosomes of cucumber. Promoter region analysis showed that the promoter regions of CsMADS-box genes contain response elements related to plant growth and development, suggesting that CsMADS-box genes may be extensively involved in plant growth and development. Different CsMADS-box genes exhibit specific high expression in roots, stems, leaves, tendrils, male flowers, female flowers, and ovaries, suggesting that these genes play crucial roles in the growth, development, reproduction and morphogenesis of cucumber. Moreover, 26, 18, 8, and 10 CsMADS-box genes were differentially expressed under high temperature, NaCl and/or silicon, downy mildew, and powdery mildew treatments, respectively. Interestingly, CsMADS07 and CsMADS16 responded to all tested stress conditions. These findings provide a reference and basis for further investigation into the function and mechanisms of the MADS-box genes for resistance breeding in cucumber.

Influence of various protection systems on the level of development of the main phytopathogens of soybean in Khabarovsk krai

The research work was carried out with the aim of determining the effectiveness of modern biological and chemical soybean protection products against pathogens of root rot and leaf and stem diseases. The work was carried out in 2016-2020, 2022-2023 in grain-soybean crop rotation in soybean crops of the Ivan Karamanov, Batya and Khabarovsky Yubilyar varieties against a natural infectious background. The main diseases in crops during the research years were root rot, septoria, and downy mildew. Pre-sowing treatment of soybean seeds can reduce the development of root rot by 1.4-2.3 times. The most effective technique in the fight against seed and soil infection in soybean crops is the treatment of seeds before sowing with chemical seed treatment agents TMTD with an application rate of 6.0 l/t, Maxim with an application rate of 2.0 l/t, Oplot and Sinclair with an application rate of 0.6 l/t, Deposit with an application rate of 1.2 l/t and Standak Top with an application rate of 1.5 l/t. The most effective protection systems against septoria were those using the complex application of Deposit with an application rate of 1.2 l/t seed treatment with Misteria with an application rate of 1.2 l/ha fungicide treatment of plants, TMTD with an application rate of 3.0 l/t with Zircon with an application rate of 40 ml/t for seed treatment with subsequent Zircon with an application rate of 10 ml/ha treatment of plants during flowering, TMTD with an application rate of 6.0 l/t (seed treatment) with Optimo with an application rate of 0.5 l/ha (plant treatment), as well as Zircon with an application rate of 40 ml/t and 10 ml/ha, Extrasol with an application rate of 2.0 l/t and 2.0 l/ha and Nutri-Fight with an application rate of 0.75 l/ha bioagents. The use of Standak Top with an application rate of 1.5 l/t as a seed treatment agent and Pictor Active with an application rate of 0.6 l/ha for treating vegetative plants reduced the incidence of downy mildew in plants by 4,6 times; TMTD for seed treatment and Optimo for spraying plants – by 3,6 times; pre-sowing TMTD with an application rate of 6.0 l/t seed treatment with subsequent Zircon with an application rate of 10 ml/ha treatment of plants during flowering – by 2,9 times, and pre-sowing seed treatment with subsequent Zircon with an application rate of 40 ml/t and 10 ml/ha treatment of plants – by 2,6 times.

A Dataset of SNPs Related to Downy Mildew Resistance in East Asian Grape Based on GBTS

Grapevine is an important commercial crop widely grown in the world. However, grapevine cultivation has long been seriously threatened by grapevine downy mildew caused by Plasmopara viticola. Screening the resistance resources and identifying the resistance germplasm is the key to improve the resistance of cultivars to P. viticola. East Asia is one of the three origin regions of wild grapevine (North America, Eurasia and East Asia), and many varieties have strong disease resistance. In present study, we selected nine wild East Asian grape vines and selected three of them with the highest P. viticola resistance to construct genetic populations. Genotyping by Targeted Sequencing (GBTS) is a high-throughput and cost-effective technology for sequencing specific genome regions, and its application in genetic population analysis and disease resistance related markers identification has revealed that may be abundant P. viticola-resistant germplasm in East Asian grapes. Our sequencing data and disease resistance grade data provided a valuable reference for mining downy mildew resistant germplasm of East Asian grape and breeding resistant varieties.

Monitoring the Progression of Downy Mildew on Vineyards Using Multi-Temporal Unmanned Aerial Vehicle Multispectral Data

Monitoring vineyard diseases such as downy mildew (Plasmopara viticola) is important for viticulture, enabling an early intervention and optimized disease management. This is crucial for disease monitoring, and the use of high-spatial-resolution multispectral data from unmanned aerial vehicles (UAVs) can allow to for a better understanding of disease progression. This study explores the application of UAV-based multispectral data for monitoring downy mildew infection in vineyards through multi-temporal analysis. This study was conducted in a vineyard plot in the Vinho Verde region (Portugal), where 84 grapevines were monitored, half of which received phytosanitary treatments while the other half were left untreated in this way during the growing season. Seven UAV flights were performed across different phenological stages to assess the effects of infection using spectral bands, vegetation indices, and morphometric parameters. The results indicate that downy mildew affects canopy area, height, and volume, restricting the vegetative growth. Spectral analysis reveals that infected grapevines show increased reflectance in the visible and red-edge bands and a progressive decline in near-infrared (NIR) reflectance. Several vegetation indices demonstrated a suitable response to the infection, with some of them being capable of detecting early-stage symptoms, while vegetation indices using red edge and NIR allowed us to track disease progression. These results highlight the potential of UAV-based multi-temporal remote sensing as a tool for vineyard disease monitoring, supporting precision viticulture and the assessment of phytosanitary treatment effectiveness.

Control of cucumber downy mildew disease under greenhouse conditions using biocide and organic compounds via induction of the antioxidant defense machinery

Cucumber as an important vegetable crop faces a variety of abiotic and biotic stresses, especially the recently appeared fungicide-resistant strains of Pseudoperonospora cubensis, the causal agent of downy mildew disease. Herein, the present study aimed to evaluate the effect of Trichoderma asperellum strain T34 as a biological commercial product and potassium phosphite (KPHI) against P. cubensis and investigate the ability of these compounds to activate the plant defense system to suppress P. cubensis infection. In two separate experiments, the foliar applications with T34 or KPHI significantly reduced the severity of downy mildew disease and the area under the disease progress curve compared to water control. Scanning electron microscopy (SEM) of P. cubensis treated with T34 and KPHI showed shrunken and distorted sporangiophores and sporangia. Moreover, two tested compounds enhanced cucumber plants’ growth and yield parameters under greenhouse conditions. The tested compounds protected the membrane permeability of infected cucumber leaves and significantly reduced electrolyte leakage (EL %) compared to water control. These findings were associated with activating enzymatic antioxidant enzymes (catalase, peroxidase, and polyphenol oxidase). Our findings suggest that T34 and KPHI can be environmentally safe alternatives to chemical fungicides to control downy mildew disease in cucumber and other cucurbit crops.

Field evaluation of new fungicides in controlling downy mildew of broccoli - A mixed-effects model analysis

Field evaluation of new fungicides in controlling downy mildew of broccoli - A mixed-effects model analysis

Relationship between sensory perceptions and volatile and phenolic compounds of fresh basil (Ocimum basilicumL.) evaluated by multivariate statistics.

Sensory and phytochemical analyses were conducted on sweet basil (Ocimum basilicum L.) to investigate the impact of volatile and phenolic compounds on aroma, taste, and flavor by mouth. A trained panel evaluated seven accessions, including 'Aroma 2', 'Nufar', 'CB19', and four breeding lines developed by Rutgers for downy mildew resistance (DMR). The basils selected for this study displayed unique sensory profiles that were chemically characterized with 21 volatile and 15 phenolic compounds using GC/MS and LC/MS, respectively. Principal component analysis revealed a two-factor model. Factor 1 described cinnamon, floral, ginger, lemon, and musty aromas, clustering with eucalyptol and many minor aromatic compounds. 'Rutgers Thunderstruck-DMR' closely aligned with these attributes. Factor 2 described an axis with clove aroma/flavor and bitter taste on one end and anise and sweet aroma/taste on the opposite end. Anise aroma/flavor was closely associated with methyl chavicol and sweet aroma/flavor. Eugenol and several phenolic acids clustered near bitter taste. However, not all phenolic acids contributed to bitterness or astringency, suggesting diverse roles in sensory perception. 'Aroma 2', 'Rutgers Passion DMR', and 'Rutgers Obsession DMR' were aligned with the clove/spicy/bitter pole of Factor 2, whereas 'CB19' was oppositely aligned with the anise/sweet pole. 'Nufar' and 'Rutgers Devotion DMR' occupied the center of the plot and were characterized as moderate in their sensory/phytochemical profiles. In conclusion, this study reveals that new varieties can be distinguished by their sensory/phytochemical profiles and compared to commercial cultivars. Further, the inclusion of phenolic compounds led to more precise sensory/phytochemical descriptions of these varieties. PRACTICAL APPLICATION: This research provides valuable insights into the aroma, flavor, and underlying phytochemistry of fresh basil, which can help improve its taste and quality for culinary use. Sensory scientists and breeders can use the tools presented in this study as a means of selecting basil varieties to identify 'off types' and enhance aroma and flavor profiles.

Heritable virus-induced germline editing in tomato.

Here, we report the successful implementation of heritable virus-induced genome editing (VIGE) in tomato (Solanum lycopersicum). We generated three transgenic tomato lines expressing Streptococcus pyogenes Cas9 (SpCas9) under the control of Cauliflower mosaic virus 35S (35S), S. lycopersicum ribosomal protein S5A (SlRPS5A), or S. lycopersicum YAO promoters (SlYAO). These three lines were tested for somatic and heritable editing using the tobacco rattle virus (TRV)-based system carrying guide RNAs (gRNAs) fused with mobile RNA sequences. TRV with gRNA targeted to Phytoene desaturase (SlPDS) and Downy mildew resistance 6 (SlDMR6) genes fused to mobile RNA sequences showed significant somatic editing efficiency in all three tomato lines expressing SpCas9. However, the progenies from the SlYAO promoter-driven SpCas9 tomato infected with TRV with gRNA targeted to SlDMR6 fused to the mobile RNA sequence resulted in monoallelic mutations with a frequency of 3%. Optimization of environmental conditions, such as reduced light intensity, significantly increased heritable editing frequencies, from 0% to 86% at the SlPDS and from 3% to 100% at the SlDMR6, including biallelic mutations. These findings underscore the use of appropriate promoters to express Cas nucleases and optimized environmental conditions to enhance heritable genome editing efficiency in tomato using VIGE. Furthermore, our method enables the generation of mutants without additional tissue culture or transformation once a SpCas9-expressing tomato line is established.

Multiplexed real-time and digital PCR tools to differentiate clades of Plasmopara viticola causing downy mildew in grapes.

In vineyards, downy mildew of grapes, caused by the oomycete pathogen Plasmopara viticola, can cause significant economic losses when left unmanaged. P. viticola is a species complex, made up of at least four clades, or cryptic species, causing disease on at least eight plant species within the family Vitaceae. In the United States, clades aestivalis, riparia, and vinifera, have been identified, as being present in the Great Lakes region on cultivated grapes. Within this study, a multiplexed TaqMan qPCR assay system capable of differentiating among these three taxa was developed using a mitochondrial gene order difference unique to Plasmopara species (cox1-atp1). The assay is needed to clearly differentiate among the closely related species as research investigates relationships between the clades and their varying hosts as well as fungicide resistance development. The multiplexed assay was validated using a panel of target and non-target samples of varying types, including leaves, ToughSpot stickers, and air sampling rods. The assay was also transferred to and optimized on a digital PCR (dPCR) platform. Air sampling rods and artificially inoculated mixed samples were tested using both qPCR and the dPCR assays to gauge utility of each. The multiplexed assays for each clade showed varying sensitivity of 10 to 1,000 fg of DNA and efficiency of 70-85% in qPCR. The dPCR sensitivity was the same, except for clade riparia, which showed a potential tenfold increase in sensitivity. These results suggest that the dPCR can serve as a more sensitive option than qPCR when trying to diagnose plant pathogens, but it is dependent on the assay. This assay system provides detection of the pathogen and classification of P. viticola clades allowing discrimination in areas growing multiple cultivated or wild grape species. This will continue to be relevant as wild hosts can potentially harbor different P. viticola clades and downy mildew is intensely managed in commercial vineyards.

Fungicide Resistance Dynamics: Knowledge from Downy Mildew Management in Japanese Vineyards

This review summarizes the mechanisms and historical development of fungicides registered for grape downy mildew control in Japan, with a particular focus on their diverse molecular targets, including cell division and mitochondrial respiration. Grapevine downy mildew is one of the most important pathogens in Japanese grapevine cultivation. Grapevine downy mildew tends to be fungicide-resistant, and in recent years, a quinone outside inhibitor (QoI) fungicide-resistant strain of downy mildew has caused extensive damage in Yamanashi Prefecture, making headlines in newspapers. Although approximately 60 fungicides have been registered for downy mildew management in Japan, many have been withdrawn due to the emergence of resistant pathogen populations. Recent challenges with resistance to quinone outside inhibitors, carboxylic acid amides, and oxysterol-binding protein inhibitors underscore the ongoing importance of resistance management in Japanese viticulture and grape downy mildew control strategies.

Revealing microbial consortia that interfere with grapevine downy mildew through microbiome epidemiology

BackgroundPlant and soil microbiomes can interfere with pathogen life cycles, but their influence on disease epidemiology remains understudied. Here, we analyzed the relationships between plant and soil microbiomes and long-term epidemiological records of grapevine downy mildew, a major disease caused by the oomycete Plasmopara viticola.ResultsWe found that certain microbial taxa were consistently more abundant in plots with lower disease incidence and severity and that the microbial community composition could predict disease incidence and severity. Microbial diversity was not strongly linked to epidemiological records, suggesting that disease incidence and severity is more related to the abundance of specific microbial taxa. These key taxa were identified in the topsoil, where the pathogen’s oospores overwinter, and in the phyllosphere, where zoospores infect leaves. By contrast, the leaf endosphere, where the pathogen’s mycelium develops, contained few taxa of interest. Surprisingly, the soil microbiota was a better predictor of disease incidence and severity than the leaf microbiota, suggesting that the soil microbiome could be a key indicator of the dynamics of this primarily aerial disease.ConclusionOur study integrates long-term epidemiological data with microbiome profiles of healthy plants to reveal fungi and bacteria relevant for the biocontrol of grapevine downy mildew. The resulting database provides a valuable resource for designing microbial consortia with potential biocontrol activity. The framework can be applied to other crop systems to guide the development of biocontrol strategies and reduce pesticide use in agriculture.

Evaluation of Resistant Cultivars for Management of Downy Mildew in Fall Cucumbers in South Deerfield, MA, 2024

Cucurbit downy mildew, caused by the oomycete pathogen Pseudoperonospora cubensis, is among the most devastating diseases of fall cucumber crops in the northeastern United States. Symptoms include angular, vein-trapped lesions on undersides of leaves that are initially water-soaked and develop fuzzy gray sporulation under humid conditions. Diseased foliage quickly becomes necrotic. This report evaluates the resistance to cucurbit downy mildew of 11 cucumber cultivars ( Cucumis sativa ‘SVCS0415’, ‘SVCS0029’, ‘SVCS0025’, ‘TSX-CU254 AS’, ‘TSX-CU252 AS’, ACU-2083’, TSX-CU253 AS’, ‘EWS-CU-059’, ‘Chaperon’, ‘Gateway’, and ‘Marketmore 76’). The trial was conducted in 2024 in South Deerfield, MA. Results from this trial will help cucumber growers select appropriate resistant cultivars for fall production, when downy mildew pressure is high.

Evaluation of Resistant Cultivars for Management of Basil Downy Mildew in South Deerfield, MA, 2024

Basil downy mildew, caused by the oomycete pathogen Peronospora belbahrii, is among the most devastating diseases of basil worldwide. Symptoms include vein-trapped lesions on undersides of leaves that are initially water-soaked and develop fuzzy gray sporulation under humid conditions. Diseased foliage becomes quickly necrotic. This report evaluates the resistance to cucurbit downy mildew of 15 basil cultivars ( Ocimum basilicum ‘Prospera Active Mia’, ‘Prospera Active Lihi’, ‘Prospera Active Noga’, ‘BA159B’, ‘BA159C’, ‘BA160’, ‘Prospera Red’, ‘Prospera CRM CG1’, ‘Prospera DMR PS5’, ‘Prospera ILL2’, ‘Giuletta’, ‘Piamia’, ‘Evi’, ‘Eleonora’, and ‘Keira’). The trial was conducted in 2024 in South Deerfield, MA. Results from this trial will help basil growers select appropriate resistant cultivars for midsummer production, when downy mildew pressure is high.

Transmission Route of Airborne Fungal Spores for Cucumber Downy Mildew

Transmission Route of Airborne Fungal Spores for Cucumber Downy Mildew

Diversity and functional features of the root-associated bacteriome are dependent on grapevine susceptibility to Plasmopara viticola

BackgroundPlant health depends on beneficial interactions between the roots and their microbiomes. Despite recent progress on the role of the grapevine microbiome, the taxonomic identity and functional traits of microbial taxa specific to healthy or Plasmopara viticola-diseased plants, as well as to the susceptible or resistant cultivar are unknown. Using metabarcoding and shotgun metagenomics sequencing, we investigated the effect of downy mildew on the root-associated microbiome (rhizospheric soil, rhizoplane and endosphere) of 41B-grafted susceptible cultivar (Chardonnay) and resistant interspecific hybrid (Voltis) at flowering and veraison stages. The impact of conventional treatment on the rhizomicrobiome assembly of Chardonnay was also evaluated.ResultsAnalyses revealed a core bacteriome shared between both susceptible and resistant cultivars. This also highlighted common functional traits between the rhizosphere and rhizoplane bacteriomes in both cultivars. A dysbiosis state was also evidenced by a loss of beneficial communities in the rhizosphere of the P. viticola-infected cultivar. Microbial genome assemblies showed functional differences between healthy and diseased plants, with a loss of Pseudomonas and Phyllobacterium taxa at veraison. This state was mainly characterized by a loss of genes involved in polyamine transport and metabolism in the susceptible cultivar. It was also marked by an increase in population evenness and total bacterial diversity, and the presence of pathogenic species in susceptible plants.ConclusionsThis study reveals distinct and overlapping bacterial communities and functional genes in the rhizospheric soil, rhizoplane and root endosphere of both susceptible and resistant grapevine cultivars to downy mildew. Microbial diversity and abundant taxa of grapevine roots are influenced by downy mildew and cultivar susceptibility. Common bacterial functions are shared among rhizocompartments of susceptible and resistant cultivars, revealing a dysbiosis state and functional signatures related to plant immunity, especially in the infected-susceptible plants.

Identification of Novel Basil Downy Mildew Resistance Genes Using De Novo Comparative Transcriptomics.

Sweet basil (Ocimum basilicum L.) production is threatened by the oomycete pathogen Peronospora belbahrii, causing basil downy mildew (BDM). BDM-resistant cultivar 'Mrihani' (MRI) was identified in a germplasm screen, and bred with BDM-susceptible 'Newton' (SB22) to produce resistant cultivars, but the molecular mechanisms conferring resistance in MRI and the progeny remained unknown. A comparative transcriptomic approach was used to identify candidate resistance genes and potential mechanisms for BDM resistance. To differentiate the host-pathogen interactions in resistant and susceptible plants, RNA samples from BDM-infected MRI and SB22 plants were harvested at four time points during the first three days of infection, with mock-inoculated controls for both genotypes. Three categories of genes uniquely transcribed in the resistant MRI upon pathogen challenge were identified: nucleotide-binding leucine-rich repeat proteins (NLRs), multi-functional receptor-like kinases (RLKs), and secondary metabolic enzymes. Validation of the top resistance candidate NLR gene confirmed its unique presence in MRI and two of four resistant MRIxSB22 F2 progeny. In MRI, pathogen challenge also induced differential regulation in members of the salicylic acid synthesis pathway, suggesting its role in BDM resistance. Overall, our study demonstrates the utility of de novo comparative transcriptomics to identify resistance genes and mechanisms in non-model crops.

Preserving the Biologically Coherent Generic Concept of Phytophthora, 'Plant Destroyer'.

Phytophthora is a long-established, well known and globally important genus of plant pathogens. Phylogenetic evidence has shown that the biologically distinct, obligate biotrophic downy mildews evolved from Phytophthora at least twice. Since, cladistically, this renders Phytophthora 'paraphyletic', it has been proposed that Phytophthora evolutionary clades be split into multiple genera (Runge et al. 2011; Crous et al. 2021; Thines et al. 2023; Thines 2024). In this letter, we review arguments for the retention of the generic name Phytophthora with a broad circumscription made by Brasier et al. (2022) and by many delegates at an open workshop organized by the American Phytopathological Society. We present our well-considered responses to this proposal in general terms and to the specific proposals for new genera; together with new information regarding the biological properties and mode of origin of the Phytophthora clades. We consider that the proposals for new genera are mostly non-rigorous and not supported by the scientific evidence. Further, given (1) the apparent lack of any distinguishing biological characteristics (synapomorphies) between the Phytophthora clades; (2) the fundamental monophyly of Phytophthora in the original Haeckelian sense; (3) the fact that paraphyly is not a justification for taxonomic splitting; and (4) the considerable likely damage to effective scientific communication and disease management from an unnecessary break-up of the genus, we report that Workshop delegates voted unanimously in favour of preserving the current generic concept and for seeking endorsement of this view by a working group of the International Commission on the Taxonomy of Fungi.

Sustainable Management of Major Fungal Phytopathogens in Sorghum (Sorghum bicolor L.) for Food Security: A Comprehensive Review.

Sorghum (Sorghum bicolor L.) is a globally important energy and food crop that is becoming increasingly integral to food security and the environment. However, its production is significantly hampered by various fungal phytopathogens that affect its yield and quality. This review aimed to provide a comprehensive overview of the major fungal phytopathogens affecting sorghum, their impact, current management strategies, and potential future directions. The major diseases covered include anthracnose, grain mold complex, charcoal rot, downy mildew, and rust, with an emphasis on their pathogenesis, symptomatology, and overall economic, social, and environmental impacts. From the initial use of fungicides to the shift to biocontrol, crop rotation, intercropping, and modern tactics of breeding resistant cultivars against mentioned diseases are discussed. In addition, this review explores the future of disease management, with a particular focus on the role of technology, including digital agriculture, predictive modeling, remote sensing, and IoT devices, in early warning, detection, and disease management. It also provide key policy recommendations to support farmers and advance research on disease management, thus emphasizing the need for increased investment in research, strengthening extension services, facilitating access to necessary inputs, and implementing effective regulatory policies. The review concluded that although fungal phytopathogens pose significant challenges, a combined effort of technology, research, innovative disease management, and effective policies can significantly mitigate these issues, enhance the resilience of sorghum production to facilitate global food security issues.

Biochemical characterization of fenugreek (Trigonella foenum-graecum L.) genotypes for downy mildew resistance

Biochemical characterization of fenugreek (Trigonella foenum-graecum L.) genotypes for downy mildew resistance

Cultivar and Training System Impact Cold-climate Seedless Table Grape Performance in the Northeastern United States

We grew eight cultivars of seedless table grape in Durham, NH, USA, from 2015 to 2021, using two training systems: Munson and vertical shoot positioning (VSP). We evaluated the vine vigor, prevalence of common diseases, yield, and postharvest fruit weight loss. We observed that cultivar significantly impacted vine vigor and the prevalence of three common diseases. One year after planting, the cultivars Thomcord, Marquis, and Lakemont had significantly less vigor than that of other cultivars, and Thomcord and Marquis both exhibited high mortality (38% and 29%, respectively). ‘Marquis’ and ‘Thomcord’ consistently showed among the highest prevalence of most diseases present in the vineyard (powdery mildew, anthracnose, and downy mildew), whereas ‘Concord Seedless’, ‘Canadice’, and ‘Mars’ consistently showed among the fewest symptoms. ‘Thomcord’ and ‘Marquis’ were removed from the experiment in 2019. For the six remaining cultivars, the cultivar, year, training system, and all interaction terms significantly affected yield. ‘Mars’ produced the highest yield throughout the study, reaching a maximum yield of over 15 kg/vine in their seventh season in the vineyard. Overall, yields of vines growing on the Munson training system were greater than those growing on the VSP system (P < 0.0001), although the advantages of growing on Munson were greater for some cultivars than for others (e.g., the yield advantages in 2021 were only 8% for ‘Lakemont’ but reached 90% for ‘Concord Seedless’ and 74.8% for ‘Vanessa’). ‘Mars’ exhibited the lowest percentage weight loss after 28 days of cold storage, which was significantly lower than that of all cultivars except Reliance. In conclusion, ‘Mars’, ‘Canadice’, ‘Vanessa’, and ‘Lakemont’ offer potential for commercial production of seedless table grapes in the northeastern United States based on producing moderate to high yields of consistently high-quality fruit; of these, ‘Mars’ showed the least susceptibility to common diseases and postharvest weight loss.