Ecological Diversity Research Articles

Missing the biodiversity for the bee: Natural land management strategies impact functional invertebrate diversity in commercial cranberry production

Abstract Simplification of agricultural environments is linked to declines in biodiversity. Improving the floral diversity within and around these areas may result in more robust and diverse ecosystems. We investigated how floral resource abundance, diversity, and species composition in a cranberry agricultural system correlated to the abundance and overall invertebrate diversity and to the abundance and diversity of specific invertebrate groups of agricultural importance (e.g. parasitoids, phytophagous taxa, pollinators and predators). This study focused on habitats immediately surrounding cranberry production and included grassy dikes under a managed system (‘dike’), and semi‐natural areas growing on the surrounding support land (‘semi‐natural’). Floral resource availability and diversity tended to be similar between habitats, while invertebrate richness, diversity and composition differed. As the availability of floral resources increased, invertebrate abundance increased but diversity decreased. Overall invertebrate community composition differed with the specific species and availability of floral resources. The habitat type and floral resource composition impacted some agriculturally important groups, as pollinator abundance was higher in the semi‐natural habitat, and parasitoid abundance varied with floral resource composition across both habitats. These results suggest that managing the structural and floral resource diversity associated with agroecosystems can help support local biodiversity. However, these systems may disproportionately benefit more common taxonomic groups. The difference in responses of individual taxonomic groups also highlights the potential tradeoffs of focusing on only a subset of biodiversity aspects.

A functionally augmented carbohydrate utilization locus from herbivore gut microbiota fueled by dietary β-glucans

Gut microbiota members from the Bacteroidota phylum play a pivotal role in mammalian health and metabolism. They thrive in this diverse ecosystem due to their notable ability to cope with distinct recalcitrant dietary glycans via polysaccharide utilization loci (PULs). Our study reveals that a PUL from an herbivore gut bacterium belonging to the Bacteroidota phylum, with a gene composition similar to that in the human gut, exhibits extended functionality. While the human gut PUL targets mixed-linkage β-glucans specifically, the herbivore gut PUL also efficiently processes linear and substituted β-1,3-glucans. This gain of function emerges from molecular adaptations in recognition proteins and carbohydrate-active enzymes, including a β-glucosidase specialized for β(1,6)-glucosyl linkages, a typical substitution in β(1,3)-glucans. These findings broaden the existing model for non-cellulosic β-glucans utilization by gut bacteria, revealing an additional layer of functional and evolutionary complexity within the gut microbiota, beyond conventional gene insertions/deletions to intricate biochemical interactions.

Barn owl site occupancy and breeding success in relation to land use and nest box characteristics

AbstractAgricultural landscapes play a crucial role in enhancing biodiversity because of their widespread presence over the Earth and their ability to encompass diverse ecosystems. Recognizing this, numerous governments are incentivizing farmers through direct payments to adopt sustainable practices, such as managing extensive pastures and meadows, planting wildflowers, or establishing hedgerows. However, the benefit of such sustainable practices on vertebrate species is not well understood. From 2018 to 2020, we investigated nest occupancy, fledging success, and clutch size of a Swiss population of barn owls (Tyto alba) with respect to nest box characteristics and the presence of extensive agriculture and urbanization in areas surrounding nest boxes. Our results revealed that extensively used pastures were positively associated with site occupancy but negatively associated with clutch size. The proportion of urban areas was negatively related to both site occupancy and clutch size. The altitude of the nest box location was negatively correlated with occupancy, and the number of nest boxes placed at the same site (either 1 or 2) was positively correlated with site occupancy. Moreover, clutch size, but not fledging success, was larger in nest boxes placed outside barns than in nest boxes placed inside barns. Based on these findings, we recommend installing nest boxes at locations <700 m in altitude and in pairs on the same barn, incorporating biodiversity promotion areas into agricultural landscapes, and avoiding dense urban areas in favor of rural zones with lower urban density. Understanding the nuanced relationships between nest box characteristics, environmental factors, and breeding success provides valuable insights for optimizing artificial nesting sites and enhancing the overall reproductive success of barn owls.

The dung beetles of Venezuela (Coleoptera: Scarabaeidae: Scarabaeinae): catalogue and updated distribution

Venezuela’s diverse land ecosystems are grouped into four major regions (coast-islands, low plains, hills and mountains), ranging from sea level up to 4978 m. The Scarabaeinae (Scarabaeidae, Coleoptera) currently encompass 278 genera and 6837 species worldwide, but are poorly inventoried in Venezuela. We reviewed the literature and the material housed at the entomological collection of the Universidade Federal de Mato Grosso (CEMT), Cuiabá, Brazil, and found 32 genera and 149 species of dung beetles as certainly present in Venezuela. Twenty-four of these species are, as far as current knowledge goes, endemic to the country, while another 34 are restricted to Venezuela and the neighbouring countries of Colombia, Brazil, Trinidad and Tobago, and Guyana. Additionally, 36 species are deemed potential inhabitants of the country, whilst 14 others previously recorded in the literature as part of the Venezuelan fauna are here concluded not to be actually present there. Complete literature is listed for each genus and species, and information on type material, material examined, worldwide distribution, and Venezuelan records is also presented.

The evolving three-dimensional landscape of human adaptation.

Over the past 3 million years, humans have expanded their ecological niche and adapted to more diverse environments. The temporal evolution and underlying drivers behind this niche expansion remain largely unknown. By combining archeological findings with landscape topographic data and model simulations of the climate and biomes, we show that human sites clustered in areas with increased terrain roughness, corresponding to higher levels of biodiversity. We find a gradual increase in human habitat preferences toward rough terrains until about 1.1 million years ago (Ma), followed by a 300 thousand-year-long contraction of the ecological niche. This period coincided with the Mid-Pleistocene Transition and previously hypothesized ancestral population bottlenecks. Our statistical analysis further reveals that from 0.8 Ma onward, the human niche expanded again, with human species (e.g., H. heidelbergensis, H. neanderthalensis, and H. sapiens) adapting to rougher terrain, colder and drier conditions, and toward regions of higher ecological diversity.

The Mycobiota of Library Books in Russia.

Abstract-Numerous studies of microorganisms isolated from the surface of cultural heritage objects, including library documents, are regularly carried out in different countries. Although the micromycete composition in each case varies, some species are constantly isolated. The structure of micromycete communities that inhabit library documents was studied in 57 cities of Russia located in seven federal districts (Northwestern, Central, Southern, Volga, Ural, Siberian, and Far Eastern). Micromycetes of 95 species from 32 genera were isolated and identified. The mycobiota of the library documents represented by Ascomycota has more than 90% of the species richness; Mucoromycota make up 3-9% and Basidiomycota, 3-4%. The Aspergillaceae family was the leading family: it accounted for 48.5-67.3% of the total species richness. In all regions, species diversity is moderate: the Shannon index ranged from 2.7 to 3.3. The Mcintosh species richness index is high everywhere (48-126), except in the Ural district (15.3). The McIntosh (0.76-0.84) and Pielow (0.80-0.91) dominance indices indicate a high level of species evenness in the mycobiota. The obtained values demonstrate the stability the document mycobiota in libraries from different regions. Significant species similarity between the districts was revealed by calculation of binary coefficients: the Jaccard coefficient was from 0.44 to 0.60; the Sørensen's qualitative measure of similarity was from 0.63 to 0.75; the quantitative similarity measure of Sørensen was from 0.44 to 0.71, and Morisita-Horn was from 0.66 to 1.0. The groups of dominant species in different regions are quite similar. The study of the ecological diversity of mycobiota on library books demonstrated the moderate diversity and the stability of the community. A high degree of similarity of taxonomic structures was established regardless of the climate conditions of the regions. Cosmopolitans characterized by high frequency of occurrence formed the major core of the library book mycobiota: Alternaria alternata, Aspergillus niger, Cladosporium cladosporioides, Mucor plumbeus, and Penicillium aurantiogriseum.

Five new species of endophytic Penicillium from rubber trees in the Brazilian Amazon.

The Amazon rainforest is the world's most diverse ecosystem, full of fauna and flora. Among the trees that make up the forest are the rubber trees of the genus Hevea (H. brasiliensis and H. guianensis), which stand out for the industrial use of latex. It was previously shown that endophytic fungi colonize the leaves, stems, and roots of Hevea spp. In this study, 47 Penicillium spp. and three Talaromyces spp. isolates were analyzed using specific DNA barcodes: internal transcribed spacers region (ITS), β-tubulin (BenA), calmodulin (CaM), and the DNA-dependent RNA polymerase II second largest subunit (RPB2) genes and additionally, for species delimitation, the genealogical concordance phylogenetic species recognition (GCPSR) criteria were applied. The phylogenetic analyses placed the Penicillium isolates into four sections Lanata-Divaricata, Sclerotiora, Citrina, and Fasciculata. The morphological and molecular characteristics resulted in the discovery of five new species (P. heveae sp. nov., P. acrean sp. nov., P. aquiri sp. nov., P. amazonense sp. nov., and P. pseudomellis sp. nov.). The five new species were also compared to closely related species, with observations on morphologically distinguishing features and colony appearances. Bayesian inference and maximum likelihood analysis have supported the placement of P. heveae sp. nov. as a sister group to P. globosum; P. acrean sp. nov. and P. aquiri sp. nov. as sister groups to P. sumatrense; P. amazonense sp. nov. closely related to isolates of P. rolfsii, and P. pseudomellis sp. nov. closely related to P. mellis. The study of endophytic Penicillium species of rubber trees and the description of five new taxa of Penicillium sect. Citrina, Lanata-Divaricata, and Sclerotiora as endophytes add to the fungal biodiversity knowledge in native rubber trees. Reports of fungi in native tropical plants may reveal taxonomic novelties, potential pathogen control agents, and producers of molecular bioactive compounds of medical and agronomic interest.

Digital twins and biodiversity: a conceptual framework of opportunities and challenge

PurposeThe intersection of digital twins and ecological diversity offers a new and complex landscape that requires interdisciplinary investigation. Recognizing the urgent need for a nuanced approach to the ethical, technological and practical challenges in this area, this paper presents a conceptual framework that serves as a roadmap for future research and policymaking.Design/methodology/approachThis study employs a conceptual methodology deeply rooted in interdisciplinary perspectives. A systematic literature review was conducted using the Scopus database to identify relevant articles. The selected articles were rigorously analyzed to derive key dimensions, which were then validated through expert panel reviews and a pilot study.FindingsOur framework identifies seven critical dimensions: Data Acquisition and Simulation, Impact Assessment, Ecological Protection and Management, Ethical and Legal Considerations, Social and Cultural Impacts, Technological Feasibility and Limitations and Policies and Regulations. These dimensions provide a comprehensive structure for understanding and addressing the intersection of digital twins and biodiversity conservation.Originality/valueThis study contributes a foundational guide for sustainable and ethical engagement between digital twins and biodiversity conservation. It offers a novel, interdisciplinary framework that integrates diverse perspectives and provides practical insights for scholars, policymakers and practitioners in this emerging field.

Compensation Mechanism for Ecological Diversity Governance in Chinese River Basins Application Based on Low-Carbon Water Quality Game Theory

Background: Taking the construction of ecological civilization as an important core of the overall layout of the “Five in One” and the coordinated promotion of the “Four Comprehensives” strategic layout, combined with the global concept of ecological environment governance, provides a new direction for the reform and innovation of China’s watershed ecological compensation system through diversified governance. Subjects and methods: This article focuses on ecological compensation in river basins, exploring collaborative cooperation models between upstream and downstream of the basin under the dual constraints of “low-carbon” and “water quality”, to achieve a “win-win” situation between the ecological environment of the basin and all parties involved in basin protection. Based on the theory of evolutionary game theory, the indicators of “carbon emissions” and “water quality” are introduced to construct a diversified ecological co-governance compensation strategy model for the watershed. By exploring the optimal strategies among various entities in the upstream and downstream of the watershed. Results: (1) In the initial stage, the optimal strategy for ecological compensation in the watershed among stakeholders cannot achieve a stable balance through their evolution; (2) In the mature stage, based on the regulation of the superior government, interest subjects will choose a mutual supervision strategy to avoid bearing high penalties, to achieve Pareto effectiveness in achieving a stable overall economy of the basin; (3) By paying for high-quality resources from downstream and implementing carbon trading among basin entities, we can promote internal resource digestion, reduce intermediate transaction costs, and deeply promote the overall green and sustainable development of the basin. Through analysis, it has been proven that the conclusion is effective, providing decision support for the diversified co-governance of watershed ecosystems. Based on building an ecological community with a shared future in the watershed, we aim to achieve the construction of a community with a shared future for mankind.

Potential and Challenges of Ecotourism Development in West Sinjai District, Sinjai Regency

One of the great potentials in Terasa Village to become an ecotourism area is a culture that has its own characteristics, mutual cooperation that is still active, and has a tourist area of Laliako Waterfall. This area has attractiveness and uniqueness, such as biophysical potential and cultural potential. The beauty of the natural panorama, the diversity of flora, fauna and diverse ecosystems and the challenges of the terrain are often the main attraction, as well as the cultural diversity of the Terasa Village community. The government can further assist the development of ecotourism by providing both tangible (capital) and intangible (security, knowledge) support. This condition encourages local communities to manage their tourism potential. One of the developments is the Laliako Waterfall tourist attraction which can be an alternative to community visits on weekends, as well as local culture that is still well preserved as one of the cultural preservation by the Terasa Village community located in Pattiro Hamlet and Laha-laha Hamlet.

Effects of Polyvinyl Chloride Microplastics on the Reproductive System, Intestinal Structure, and Microflora in Male and Female Mice

The pervasive use of plastics in numerous industrial sectors has resulted in the circulation of microplastics across diverse ecosystems and food chains, giving rise to mounting concerns regarding their potential adverse impacts on biological systems and the environment. The objective of this experiment was to investigate the distinct effects of microplastic-polyvinyl chloride (PVC) exposure on the reproductive system, intestinal tissue structure, and intestinal microbial flora of both male and female mice. A total of 24 4-week-old Kunming mice were randomly assigned to one of four groups: male control group (CM), female control group (CF), male PVC test group (PVCM), and female PVC test group (PVCF) (n = 6). The findings revealed that in terms of the reproductive system, the PVCM group exhibited an impaired testicular structure with an irregular arrangement and a significant reduction in spermatogonia, spermatocytes, and spermatozoa within the seminiferous tubules (p < 0.01). The PVCF group exhibited a notable decrease in ovarian follicles (p < 0.01), accompanied by a reduction in uterus volume, fallopian tube volume, and muscle layer thickness, all of which also decreased significantly (p < 0.01). In comparison to the control groups, exposure to PVC resulted in a reduction in the width and height of the intestinal villi, accompanied by an increase in crypt depth. This led to a significant alteration in the ratio of villus height to crypt depth (V/C) (p < 0.01). Moreover, a reduction in microbial species diversity was observed within both the PVCM and PVCF groups; additionally, it was accompanied by contrasting changes in relative abundance and functional gene profiles among the major intestinal flora constituents. In summary, the findings indicate that PVC induces damage to both male and female mice reproductive and digestive systems, further exhibiting notable sex-dependent effects on mouse intestinal microflora composition, which correlates significantly with its impact on reproductive organs.

Deepening the Decade: Collaborative Action for Advancing Deep‐Ocean Science and Policy in the United Nations Decade of Ocean Science for Sustainable Development

AbstractThe current United Nations Decade of Ocean Science for Sustainable Development (2021–2030; hereafter, the Decade) offers a unique opportunity and framework to globally advance ocean science and policy. Achieving meaningful progress within the Decade requires collaboration and coordination across Decade Actions (Programs, Projects, and Centres). This coordination is particularly important for the deep ocean, which remains critically under‐sampled compared to other ecosystems. Despite the limited sampling, the deep ocean accounts for over 95% of Earth's habitable space, plays a crucial role in regulating the carbon cycle and global temperatures, and supports diverse ecosystems. To collectively advance deep‐ocean science, we gathered representatives from 20 Decade Actions that focus at least partially on the deep ocean. We identified five broad themes that aim to advance deep‐ocean science in alignment with the Decade's overarching 10 Challenges: natural capital and the blue economy, biodiversity, deep‐ocean observing, best practices in data sharing, and capacity building. Within each theme, we propose concrete objectives (termed Cohesive Asks) and milestones (Targets) for the deep‐ocean community. Developing these Cohesive Asks and Targets reflects a commitment to better coordination across deep‐ocean Decade Actions. We aim to build bridges across deep‐ocean disciplines, which encompass natural science, ocean observing, policy, and capacity development.

An exploration of the impact of phylogenetic tree structure on NTI and βNTI estimates of community assembly

The nearest taxon index (NTI) and beta nearest taxon index (βNTI) are popular metrics used to infer community assembly in a variety of ecosystems. However, the dependence these metrics have on the underlying phylogenetic tree is often overlooked and can potentially lead to erroneous conclusions. In this paper, we investigated how misclassified sequences in the phylogenetic tree and reduction of the metacommunity richness affect estimates of the assembly processes. We estimated assembly processes in two datasets investigating fish-microbiota interactions. We found that non-bacterial misclassified sequences can drastically change the interpretation of assembly processes. NTI consistently increased after misclassified sequences were removed, which changed the ecological interpretation for 82.8% of the communities in one of the datasets. Similarly, βNTI was strongly affected by misclassified sequences in the phylogenetic tree, changing the ecological interpretation for 44.7% (Dataset 1) and 6.3% (Dataset 2) of the community comparisons. Lower metacommunity richness generally resulted in decreased NTI and increased βNTI, but the effect sizes were small and generally did not result in a change in ecological interpretation. Based on these findings, we recommend: (1) constructing and visually inspecting a phylogenetic tree for 16 S rRNA gene amplicon datasets to identify and remove misclassified sequences, and (2) evaluating the robustness of community assembly conclusions by performing analyses with varying metacommunity richness and clearly specifying the metacommunity used for the null model approach.

Evaluation of Compost and Manure Amendments for Suppressing Heterodera glycines.

Soybean cyst nematode (SCN) is a major pest of soybean crops, causing significant yield losses and economic impact. Current management strategies primarily rely on resistant varieties, cover crops, and seed treatments. In addition, there is a growing interest in developing sustainable, ecologically based approaches to integrate SCN risk reduction into soybean production systems. This study aimed to evaluate the efficacy of various compost and manure amendments in suppressing SCN populations and promoting soybean productivity. An in vitro egg hatching assay was conducted to screen the inhibitory effects of different compost and manure extracts on SCN egg hatching. Results indicated that poultry manure, Layer Ash Blend, and swine manure extracts significantly inhibited SCN hatching compared with other treatments across multiple time points. Greenhouse trials further validated the effectiveness of Layer Manure, poultry manure, High Carbon Dairy Doo, and Seed Starter 101 in suppressing SCN cysts, eggs, and juveniles. A field microplot trial confirmed the potential of Layer Ash Blend and poultry manure in SCN management, with significant reductions in SCN populations and increased soybean yields. The study also investigated the impact of these amendments on promoting the population of bacterivorous and frugivorous nematodes, contributing to a biological diverse soil ecosystem. Overall, the results indicate that amending SCN-infested soil with specific compost or manure formulations can effectively suppress nematode populations while improving soybean productivity. These findings contribute to the development of sustainable strategies for SCN management in soybean production systems.

Valuing ecosystem services of community gardens in developing countries: a case study of Dezful City in Iran

Allotment (AGs) and community gardens (CGs) are types of urban gardens that provide diverse ecosystem services (ESs) such as climate regulation, beautiful landscapes, biodiversity and food security. Despite this, there are no AGs or CGs in many developing countries such as Iran. One of the main reasons for the lack of development of CGs in developing countries is the lack of proper understanding by policy-makers and citizens regarding the economic value of the ESs provided and the lack of disclosure of the benefits of its construction in urban areas. The purpose of this study is to analyse the preferences of citizens, to examine the existence of a potential market, to estimate the willingness to pay (WTP) for the creation and operation of CG services and to investigate the factors affecting residents’ WTP in Dezful, Iran. For this purpose, a choice experiment, based on the stated preferences valuation approach, was applied through interviews with 170 respondents living in Dezful urban area. The data were collected during the autumn of 2022 and analysed using conditional logit model. The results indicated that the responding citizens are willing to pay 4.57 USD per month to create CG and operate its ESs in Dezful. Amongst the attributes and ESs of the community garden, citizens valued cultural services the most, followed by provisioning services. In addition, the variables “age”, “being native”, “education”, “household expenditure level” and “awareness and recognition of CG ecosystem services” had a significant effect on WTP. According to the results, it is suggested to follow the policy of creating CGs with the participation of citizens instead of focusing only on the creation of urban green space with a government budget and heavy financial burden. CGs, while providing diverse ESs, can generate sustainable incomes for municipalities and accelerate the movement toward sustainable urban development.

Taxonomic insights into ethereum smart contracts by linking application categories to security vulnerabilities.

The expansion of smart contracts on the Ethereum blockchain has created a diverse ecosystem of decentralized applications. This growth, however, poses challenges in classifying and securing these contracts. Existing research often separately addresses either classification or vulnerability detection, without a comprehensive analysis of how contract types are related to security risks. Our study addresses this gap by developing a taxonomy of smart contracts and examining the potential vulnerabilities associated with each category. We use the Latent Dirichlet Allocation (LDA) model to analyze a dataset of over 100,040 Ethereum smart contracts, which is notably larger than those used in previous studies. Our analysis categorizes these contracts into eleven groups, with five primary categories: Notary, Token, Game, Financial, and Blockchain interaction. This categorization sheds light on the various functions and applications of smart contracts in today's blockchain environment. In response to the growing need for better security in smart contract development, we also investigate the link between these categories and common vulnerabilities. Our results identify specific vulnerabilities associated with different contract types, providing valuable insights for developers and auditors. This relationship between contract categories and vulnerabilities is a new contribution to the field, as it has not been thoroughly explored in previous research. Our findings offer a detailed taxonomy of smart contracts and practical recommendations for enhancing security. By understanding how contract categories correlate with vulnerabilities, developers can implement more effective security measures, and auditors can better prioritize their reviews. This study advances both academic knowledge of smart contracts and practical strategies for securing decentralized applications on the Ethereum platform.

Ecological assessment and diversity of woody plants in plantations of frankincense trees (Boswellia papyrifera (Del.) Hochst.) in AlGerri Forest, Blue Nile State, Sudan

هدفت هذه الدراسة إلى تقييم تنوع أنواع الشجيرات والأشجار داخل مزارع اشجار اللبان (Boswellia papyrifera (Del.) Hochst.) في منطقة القري بولاية النيل الأزرق. 18 نمازج دائرية منتظمة حددت لاخذ و تسجيل البيانات تبلغ مساحتة الواحد منها 0.1 هكتار. (نصف القطر = 17.84) مع مسافات تباعد تبلغ 100 متر بين كل نموزج و الاخر، تم استخدامها لجمع البيانات. تم تحديد ما مجموعه 13 نوعًا خشبيًا تنتمي إلى 7 عائلات في مجموعتين من مزارع اشجار اللبان. ساد نوع اشجار اللبان (Boswellia papyrifera) على الأنواع الخشبية في جميع مجتمعات الغطاء النباتي، وتليها انواع الهبيل (Combretum hartmannianum) و الطلح (Acacia seya). كان مؤشرا التنوع في شانون (H) للمجتمع (1) والمجتمع (2) 0.328 و 0.429 على التوالي. كانت قيم مؤشر Pielous Evenness (E) في المجتمع (1) المجتمع (2) 0.344 و 0.429 على التوالي. كثافة المجتمع (1) والمجتمع (2) 609.1 و 309.08 نبات / هكتار. على التوالى. أظهر توزيع الأنواع الخشبية أن 61.54٪ من الأنواع تتواجد في تجمعات في توزيعها و 38.46٪ من الأنواع موزعة عشوائياً. تم استخدام التحليل العنقودي المستند إلى الرابط الفردي Bray-Curtis لتقييم التشابه بين تكوين الأنواع الخشبية عبر 18 نموزج عينة.

Reexploring Rice Germplasm: Enlightening a New Path Towards Abiotic Stress-resilient Crop

Oryza sativa, the commonly cultivated rice, is one of the most important crops for human consumption, but is also a crop whose production system is increasingly threatened by the current climate changes. Due to its intensive water requirement rice faces a lot of challenges due to abiotic stresses like drought, heat stress, salinity, flooding etc. Drought is the major abiotic stress to rice grain production under unpredictable and changing environmental conditions. Traits supporting drought resistance are limited in cultivars, while wild rice species like O. glaberrima, O. longistaminata, O. rufipogon and O. meridonalis represent an important reservoir of useful genes for this trait which can be exploited to develop varieties tolerant to drought. Similarly O. australiensis, O. coarctata and O. rufipogon serve as an important sources for salinity-tolerant genes. O. rufipogon, O. meridonalis, O. australiensis comprise a collection of accessions that are reservoirs for heat-tolerant genes. Although many efforts have resulted in breeding rice varieties that are relatively tolerant to their local environments; climate change and population growth are expected to soon require a new, rapid generation of stress-tolerant rice germplasm. Diversity studies suggest that the current diversity within rice varieties may not be sufficient for generation of highly resistant or tolerant varieties for the unfavorable growing conditions. All the efforts to design an ideotype to increase production and productivity has been attempted previously, rice self-sufficiency can be attained only if high production and productivity is coupled with a high degree of tolerance and resistance to biotic and abiotic stress factors. Since it has been found that wild rice relatives serve as a hub for many desirable genes conferring tolerance or resistance to specific stresses, the present article is focused on provide an overview of different wild ancestors of rice which can be exploited to identify suitable donors for gene of interest, their ecological and genomic diversity and the variation observed in wild Oryza species for different stress tolerance traits. Such identified traits can be incorporated in modern day breeding programmes through marker assisted breeding to develop cultivable varieties with desired tolerance to abiotic stresses or transfer such traits to modern day cultivars through backcross breeding.

Adverse Outcomes Following Exposure to Perfluorooctanesulfonamide (PFOSA) in Larval Zebrafish (Danio rerio): A Neurotoxic and Behavioral Perspective.

Toxicity mechanisms of per- and polyfluoroalkyl substances (PFASs), a chemical class present in diverse ecosystems, as well as many of their precursors, have been increasingly characterized in aquatic species. Perfluorooctanesulfonamide (PFOSA, C8H2F17NO2S) is a common precursor of perfluorooctane sulfonic acid (PFOS), a long-chain PFAS. Here, we assessed sub-lethal endpoints related to development, oxidative stress, transcript levels, and distance moved in zebrafish embryos and larvae following continuous exposure to PFOSA beginning at 6 h post-fertilization (hpf). PFOSA decreased survival in fish treated with 1 µg/L PFOSA; however, the effect was modest relative to the controls (difference of 10%). Exposure up to 10 µg/L PFOSA did not affect hatch rate, nor did it induce ROS in 7-day-old larvae fish. The activity of larval fish treated with 100 µg/L PFOSA was reduced relative to the solvent control. Transcripts related to oxidative stress response and apoptosis were measured and BCL2-associated X, apoptosis regulator (bax), cytochrome c, somatic (cycs), catalase (cat), superoxide dismutase 2 (sod2) were induced with high concentrations of PFOSA. Genes related to neurotoxicity were also measured and transcript levels of acetylcholinesterase (ache), elav-like RNA binding protein 3 (elavl3), growth-associated protein 43 (gap43), synapsin II (syn2a), and tubulin 3 (tubb3) were all increased in larval fish with higher PFOSA exposure. These data improve our understanding of the potential sub-lethal toxicity of PFOSA in fish species.

Issue agenda-setting in the voice to parliament referendum: using big data to explain voice discourse on traditional and social media

ABSTRACT This article explores issue agenda-setting in Australia's 2023 Voice to Parliament referendum, analysing the dynamics of Yes and No campaigns in a diverse media ecosystem. We identify influential messages across this complex media environment using soft clustering of sentence embeddings from large language models (LLMs). Drawing on 3.3 million documents and over 10 million Voice specific sentences from mainstream and social media sources, we identify the narrative configurations of the campaigns, their audience size and engagement. Overall, the Yes campaign dominated with more diverse messaging and greater audience size and engagement than No. Yet, the No case prevailed. We find the narrower, mostly negative No campaign helped sustain the issue agenda both online and offline, forcing Yes to respond to it. The findings also challenge echo chamber theory, revealing most unique topics contained content from both #Yes and #No perspectives, indicating robust public engagement rather than isolated filter bubbles.