Environmental variables like elevation affect species composition, diversity, distribution, density, and horizontal and upward growth. Ecologists are constantly working to better understand how species diversity varies along elevational gradients, particularly in mountainous ecosystems. Therefore, the purpose of this research was to examine the species’ horizontal and vertical structural diversity along the Harego Mountain Forest’s elevational gradient. The area was categorized into lower, middle, and upper elevations. A total of 67 (20 m × 20 m) plots were created along gradients of elevation 2,079–2,516 meters above sea level (m a.s.l.). Information for floristic composition, diversity, stand structure, and environmental variables were measured and recorded for each plot over the three elevational gradients. Data on anthropogenic disturbances were visually evaluated for every plot in every gradient of elevation. For the diversity analysis, Hill’s diversity statistics were employed. To find significant variations between the three elevational gradients in terms of Hill’s diversity number, stand structure, and environmental variables, the one-way analysis of variance with SPSS version 26 at the 0.05 level of significance was carried out. The finding revealed that 50 woody plants that belonged to 35 families and 44 genera in the 67 sample plots with an elevation of 2,079 to 2,516 were identified. Shrubs were dominant in each elevational gradient. Species richness, abundance, and Hill’s diversity number were all significantly ( p < 0.05 ) greater in the upper elevational gradient of the forest. On the other hand, all stand structures were significantly ( p < 0.05 ) higher in the middle elevational gradient. The effect of anthropogenic disturbances and environmental variables were clearly observed in the lower and upper elevational gradients than in the middle elevation. As a result, there were fewer seedlings, saplings, trees, and shrubs in the gradients of lower and higher elevations. For the conservation of the forest, it is crucial to pay special attention to biotic elements at lower elevations and abiotic factors at higher elevations. Accordingly, involving the local community in forest management, reducing anthropogenic pressure in and around the Harego Mountain Forest through tree planting in farmlands and woodlots and implementing physical soil and water conservation structures are recommended.
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