Disruption Of Communication Research Articles

Evaluation of the relationship between retinal nerve layer thickness and corpus callosum atrophy in MRI with memory impairment in patients with multiple sclerosis.

ABSTRACTIntroduction:Multiple sclerosis (MS) is a chronic neurological disease in which demyelination and loss of axons lead to disruption of communication between neurons in the central nervous system. Cognitive impairment occurs in a significant proportion of patients with MS. Therefore, the aim of this study was to investigate the relationship between retinal nerve fiber layer (RNFL) thickness and corpus callosum atrophy in magnetic resonance imaging with memory disorders in patients with MS.Methods:This descriptive analytical study was performed on patients with a diagnosis of relapsing–remitting multiple sclerosis referred to the clinic of Ali Ibn Abi Talib Hospital in Zahedan, Iran. An information form that includes a Mini–Mental State Examination was first prepared, by which the patient’s memory impairment is measured. After recording the data, the data were collected using an information form and finally analyzed by SPSS software version 22 using an independent t-test.Results:In this study, 80 patients with MS primary progressive multiple sclerosis were included in the study, of which 53 were female and 27 were male. The mean age of patients was 45.1 ± 5.9 years, which did not show a statistically significant difference (P = 0.536). The mean RNFL thickness in patients with memory impairment was significantly lower than that in patients without memory impairment. The mean corpus callosum thickness was found to be significantly lower in patients with memory impairment than in patients without memory impairment.Conclusion:All in all, the results of this study showed that the thickness of RNFL and the corpus callosum in patients with memory impairment was significantly lower than that in patients without memory impairment.

Ameliyathane Hemşirelerinin COVID-19 Pandemisindeki Deneyimleri: Nitel Bir Çalışma

Objective: This study was conducted to examine the experiences of operating room nurses care for COVID-19 positive and suspected patients during the surgical process. Material and Methods: This study was conducted qualitatively with operating room nurses between 01-31 May 2021. 22 operating room nurses were interviewed face-to-face-in-depth in the break room of the operating room. Data were analyzed by Colaizzi's seven-step method. The Consolidated Criteria for Reporting Qualitative Research checklist was used in reporting this research. Results: Three themes were determined: Difficulties experienced in the operating room during the COVID-19 epidemic, effect of the COVID-19 epidemic on the operating room organization and the effect of the risk of contamination on the surgical team communication. 7 sub-themes were identified as fear of catching and transmitting the infection, equipment problems, difficulties in the management of emergency cases, operation and working times, changes in the patient transfer line and operating room, social isolation and reduced communication, and fear of medical error. Conclusion: This study reveals difficulties experienced by nurses due to COVID-19, effect of the pandemic on the operating room organization and nurses, and effect of the risk of transmission on the surgical team communication. Nurses have fear of catching and transmitting infection in the operating room, lack of equipment, and experience anxiety in managing emergency cases. The pandemic caused changes in the organization of the operating room. Besides, nurses experienced social isolation and team communication decreased, the use of equipment disrupted communication with the surgical team, and this situation led to fear of medical error.

Pinning Decision in Interconnected Systems with Communication Disruptions under Multi-Agent Distributed Control Topology

In this study, we propose a decision-making strategy for pinning-based distributed multi-agent (PDMA) automatic generation control (AGC) in islanded microgrids against stochastic communication disruptions. The target microgrid is construed as a cyber-physical system, wherein the physical microgrid is modeled as an inverter-interfaced autonomous grid with detailed system dynamic formulation, and the communication network topology is regarded as a cyber-system independent of its physical connection. The primal goal of the proposed method is to decide the minimum number of generators to be pinned and their identities amongst all distributed generators (DGs). The pinning-decisions are made based on complex network theories using the genetic algorithm (GA), for the purpose of synchronizing and regulating the frequencies and voltages of all generator bus-bars in a PDMA control structure, i.e., without resorting to a central AGC agent. Thereafter, the mapping of cyber-system topology and the pinning decision is constructed using deep-learning (DL) technique, so that the pinning-decision can be made nearly instantly upon detecting a new cyber-system topology after stochastic communication disruptions. The proposed decision-making approach is verified using a 10-generator, 38-bus microgrid through time-domain simulation for transient stability analysis. Simulations show that the proposed pinning decision making method can achieve robust frequency control with minimum number of active communication channels.

Effective Communication Management: A Factor for Development of International Companies in Our Day

The purpose of communication management at company level is to create and exploit all the categories of company assets by building up the communication space in a way to achieve the company competitiveness. Therefore, the quality of communication management in companies has become an indicator of their ability to create an internal and external environment that will help them gain the necessary market advantages. 
 The article contains results of an analysis of the phenomenon of communication management and causes of disruptions of effective communications in international companies. A review of previous research shows that international companies operating in multicultural environments face communication barriers affecting the performance of international partners. But once built in a right manner, communication management enables to overcome such cross-cultural barriers. When the peculiarities of cross-cultural communications are accounted for, it will allow to predict the behavior of foreign partners and avoid mistakes in management of international organizations and projects. Two aspects have critical importance for the prevention or quick settlement of conflicts: (i) a cautious and balanced strategic policy of company management, the readiness to grasp the causes of conflicts and change policy schemes to achieve a reasonable compromise, flexibility and adaptability of an organizational structure; (ii) deep awareness of the cultural environments in which an organization operates, and of the cultural origins of its staff. 
 It is concluded that due consideration for the cultural diversity can strengthen mutual understanding between the partners in communication. It is shown that communication management has impact on the organizational communication climate, whereas the latter have implications for future performance of international companies. The organizational communication climate is a proxy of the relationship between communication management and prevention of conflicts.

Nuclear Architecture Disruption During Aging Causes Misexpression of Genes Lacking CpG Islands

Abstract Global disorganization of chromatin architecture, characterized by disrupted nuclear lamina and associated heterochromatin, is commonly observed in various aging contexts, including premature aging diseases, cellular senescence, and normative aging. Although these conserved structural changes have been reported for over two decades, their impact on transcription and contribution to age-related degenerative changes remain unclear. Here we show that genes not associated with CpG islands (CGI- genes), which form heterochromatin when transcriptionally silent, are globally misexpressed in aged nuclei with disrupted chromatin architectures. Our data also show that CGI- gene misexpression is a direct outcome of nuclear architecture disruption. Notably, CGI- gene misexpression explains the molecular basis of various defects observed during aging, including loss of cellular identity and increased noises in transcription. We also show that uncontrolled secretory phenotypes commonly observed during aging are largely attributable to CGI- gene misexpression, which drives disruption of intercellular communication and fuel chronic inflammation in aged tissues. Our large-scale meta-analysis further demonstrates that CGI- gene misexpression is a common feature of mammalian aging and age-associated diseases. Interestingly, CGI- gene misexpression can be suppressed by anti-aging interventions. Our study suggests that age-associated CGI- gene misexpression is a novel biomarker of physiological aging which offers an effective therapeutic target for delaying or ameliorating degenerative changes associated with aging.

Nuclear Architecture Disruption During Aging Causes Misexpression of Genes Lacking CpG Islands

Diacylglycerol O-acyltransferase 2 is a transmembrane protein encoded by the DGAT2 gene that functions in lipid metabolism, triacylglycerol synthesis, and lipid droplet regulation. Since cancer cells exhibit altered lipid metabolism, it has been proposed that mutations in DGAT2 may contribute to this state. Using data from the Catalogue of Somatic Mutations in Cancer (COSMIC), we analyzed all reported DGAT2 mutations in human cancers. Bioinformatics analyses were performed to highlight the connections between age, pathogenicity, and cancer tissue type. Mutations are generally associated with samples from older individuals, except for those in glioblastomas which occur earlier. We also found that several DGAT2 mutations fall within the catalytic site of the enzyme and may affect enzyme function. Thus, these mutations may contribute to altered cancer metabolism. We identified D222V as a mutation hotspot neighboring a previously discovered Y223H mutation that causes Axonal Charcot-Marie-Tooth disease. Remarkably, Y223H has not been detected in cancers indicating it is inhibitory to cancer progression. Further analysis showed that most mutations do not affect DGAT2 gene expression suggesting this change is not a major contributor to cancer development. Intriguingly, although most cancers are characterized by low DGAT2 gene expression, some show high expression levels, indicating that, at least in certain cases, over-expression is not inhibitory to cellular proliferation. This work uncovers unknown roles of DGAT2 in cancers and suggests that its function may be more complex than previously appreciated.

The Disinformation Age: Politics, Technology, and Disruptive Communication in the United States. Edited by W. Lance Bennett and Steven Livingston. New York: Cambridge University Press, 2020. 323p. $99.99 cloth, $29.99 paper.

The Disinformation Age: Politics, Technology, and Disruptive Communication in the United States. Edited by W. Lance Bennett and Steven Livingston. New York: Cambridge University Press, 2020. 323p. 29.99 paper. - Volume 19 Issue 4

Disrupted Market Relations in Agriculture in North Macedonia: the COVID-19 Crisis

Abstract The COVID-19 crisis has stressed the importance of, and need for, a stable and functional food system, able to provide consumers with a continuous supply of quality food at affordable prices. The pandemic has also highlighted the role of agricultural producers in the food supply chain, with small farmers being the most vulnerable category. This global crisis has actually deepened the problems faced by farmers, especially those on the verge of sustainability. The purpose of this analysis was to provide an overview of the disrupted links in agriculture, as a result of the initial COVID-19 crisis. A survey was carried out with 91 farmers in North Macedonia. Moreover, in-depth interviews were conducted with selected traders and processors. In general, farmers assessed that the crisis negatively affected their economic operations. The critical parts of the supply chain derive from the disrupted link between farmers and traders/processors, lack of field technical support resulting from the mobility restrictions, uncertainty in cooperation, lower production quantities, and varying quality of the agricultural products. Availability and cost of seasonal workforce was also pronounced as an issue. The dominant family farms small-scale structure and lack of aggregation in the sector challenged the resilience and effective response to the crisis. From buyers’ and processors’ perspective, the crisis effect was marked through the disrupted communication with farmers, low awareness of the need for change, and drastic decline in the HORECA channel sales. The agricultural and rural policy is designed to address measures adjusted to the needs of the key actors in the sector. The priority set of measures should support the food supply chain, enhancing farmers’ networking and aggregation, and on-farm and off-farm diversification, along with improved communication, information systems and digitalisation. Improving productivity and competitiveness remains an effective strategy for sustainable operations, greater resilience and risk adaptation in crises such as the pandemic.

Coronal mass ejections and high-speed solar wind streams effect on HF ionospheric communication channel

This article analyzes the degree of solar coronal mass ejections and high-speed solar wind streams influence on the ionospheric communication channel in the short-wavelength range. Regularities in the coronal mass ejections influence on the parameters of the ionosphere are revealed. It is shown that there is a decrease in the values of the used differential parameter of critical frequency of the ionosphere F2 layer after the onset of coronal mass ejections of the loop type, while no significant changes are observed from other types of coronal mass ejections. The contribution of the high-speed solar wind flux to the features of the behavior of ionospheric parameters is demonstrated. Deviations of critical frequency and maximum observed frequency of the ionosphere F2 layer indicate a change in conditions in the ionosphere, leading to disruption of radio communication in the short-wavelength range. The results of ground-based measurements of the ionospheric plasma parameters were obtained by the methods of oblique and vertical sounding of the ionosphere. The use of the method of oblique sounding made it possible to obtain data on the state of the ionosphere where there are no vertical sounding stations.

Sex-specific effects of metformin and liraglutide on renal pathology and expression of connexin 45 and pannexin 1 following long-term high-fat high-sugar diet

The comparative effects of the two commonly used antidiabetic drugs metformin and liraglutide on renal pathology and expression of connexin 45 (Cx45) and pannexin 1 (Panx1) in adult obese rats fed high-fat high-sugar diet (HFHSD) were studied. Considering recent data on the profound influence of sex on metformin and liraglutide effects, we compared the effects of both drugs between male and female animals. 44-week-old Sprague-Dawley rats were separated into 4 groups that were fed: standard diet, HFHSD, HFHSD treated with metformin (s.c., 50 mg/kg/day) and HFHSD treated with liraglutide (s.c., 0.3 mg/kg/day). Treatment with metformin or liraglutide lasted for 14 weeks. Histology and immunohistochemistry were performed to quantify renal pathological changes and Cx45 and Panx1 expression. HFHSD caused thickening of the Bowman’s capsule (BC). Both metformin and liraglutide failed to ameliorate the BC thickening; metformin even worsened it. Effects on the tubulointerstitial fibrosis score, BC thickness and Cx45 and Panx1 expression were sex-dependent. We found a 50% increase in mitochondria in proximal tubules of metformin- and liraglutide-treated HFHSD-fed rats, but these effects were not dependent on the sex. This is a first study showing that the effects of metformin and liraglutide on kidney pathology in rats fed HFHSD are mostly sex-dependent and that these effects are not necessarily beneficial. Both drugs changed the Cx45 and Panx 1 expression; hence their effects could be related to amelioration of disruptions in intercellular communication.

Temporal disruption of neuromuscular communication and muscle atrophy following noninvasive ACL injury in rats.

Many patients with anterior cruciate ligament (ACL) injuries have persistent quadriceps muscle atrophy, even after considerable time in rehabilitation. Understanding the factors that regulate muscle mass, and the time course of atrophic events, is important for identifying therapeutic interventions. With a noninvasive animal model of ACL injury, a longitudinal study was performed to elucidate key parameters underlying quadriceps muscle atrophy. Male Long-Evans rats were euthanized at 6, 12, 24, or 48 h or 1, 2, or 4 wk after ACL injury that was induced via tibial compression overload; controls were not injured. Vastus lateralis muscle size was determined by wet weight and fiber cross-sectional area (CSA). Evidence of disrupted neuromuscular communication was assessed via the expression of neural cell adhesion molecule (NCAM) and genes associated with denervation and neuromuscular junction instability. Abundance of muscle RING-finger protein-1 (MuRF-1), muscle atrophy F-box (MAFbx), and 45 s pre-rRNA along with 20S proteasome activity were determined to investigate mechanisms related to muscle atrophy. Finally, muscle damage-related parameters were assessed by measuring IgG permeability, centronucleation, CD68 mRNA, and satellite cell abundance. When compared with controls, we observed a greater percentage of NCAM-positive fibers at 6 h postinjury, followed by higher MAFbx abundance 48 h postinjury, and higher 20S proteasome activity at 1 wk postinjury. A loss of muscle wet weight, smaller fiber CSA, and the elevated expression of run-related transcription factor 1 (Runx1) were also observed at the 1 wk postinjury timepoint relative to controls. There also were no differences observed in any damage markers. These results indicate that alterations in neuromuscular communication precede the upregulation of atrophic factors that regulate quadriceps muscle mass early after noninvasive ACL injury.NEW & NOTEWORTHY A novel preclinical model of ACL injury was used to establish that acute disruptions in neuromuscular communication precede atrophic events. These data help to establish the time course of muscle atrophy after ACL injury, suggesting that clinical care may benefit from the application of acute neurogenic interventions and early gait reloading strategies.

Psychological Stress as a Risk Factor for Accelerated Cellular Aging and Cognitive Decline: The Involvement of Microglia-Neuron Crosstalk.

The relationship between the central nervous system (CNS) and microglia is lifelong. Microglia originate in the embryonic yolk sac during development and populate the CNS before the blood-brain barrier forms. In the CNS, they constitute a self-renewing population. Although they represent up to 10% of all brain cells, we are only beginning to understand how much brain homeostasis relies on their physiological functions. Often compared to a double-edged sword, microglia hold the potential to exert neuroprotective roles that can also exacerbate neurodegeneration once compromised. Microglia can promote synaptic growth in addition to eliminating synapses that are less active. Synaptic loss, which is considered one of the best pathological correlates of cognitive decline, is a distinctive feature of major depressive disorder (MDD) and cognitive aging. Long-term psychological stress accelerates cellular aging and predisposes to various diseases, including MDD, and cognitive decline. Among the underlying mechanisms, stress-induced neuroinflammation alters microglial interactions with the surrounding parenchymal cells and exacerbates oxidative burden and cellular damage, hence inducing changes in microglia and neurons typical of cognitive aging. Focusing on microglial interactions with neurons and their synapses, this review discusses the disrupted communication between these cells, notably involving fractalkine signaling and the triggering receptor expressed on myeloid cells (TREM). Overall, chronic stress emerges as a key player in cellular aging by altering the microglial sensome, notably via fractalkine signaling deficiency. To study cellular aging, novel positron emission tomography radiotracers for TREM and the purinergic family of receptors show interest for human study.

Understanding Dialogue: Language Use and Social Interaction

Understanding Dialogue: Language Use and Social Interaction

Impaired Sensorimotor Adaption in Schizophrenia in Comparison to Age-Matched and Elderly Controls

Background: The “cognitive dysmetria hypothesis” of schizophrenia proposes a disrupted communication between the cerebellum and cerebral cortex, resulting in sensorimotor and cognitive symptoms. Sensorimotor adaptation relies strongly on the function of the cerebellum. Objectives: This study investigated whether sensorimotor adaptation is reduced in schizophrenia compared with age-matched and elderly healthy controls. Methods: Twenty-nine stably treated patients with schizophrenia, 30 age-matched, and 30 elderly controls were tested in three motor adaptation tasks in which visual movement feedback was unexpectedly altered. In the “rotation adaptation task” the perturbation consisted of a rotation (30° clockwise), in the “gain adaptation task” the extent of the movement feedback was reduced (by a factor of 0.7) and in the “vertical reversal task,” up- and downward pen movements were reversed by 180°. Results: Patients with schizophrenia adapted to the perturbations, but their movement times and errors were substantially larger than controls. Unexpectedly, the magnitude of adaptation was significantly smaller in schizophrenia than elderly participants. The impairment already occurred during the first adaptation trials, pointing to a decline in explicit strategy use. Additionally, post-adaptation aftereffects provided strong evidence for impaired implicit adaptation learning. Both negative and positive schizophrenia symptom severities were correlated with indices of the amount of adaptation and its aftereffects. Conclusions: Both explicit and implicit components of sensorimotor adaptation learning were reduced in patients with schizophrenia, adding to the evidence for a role of the cerebellum in the pathophysiology of schizophrenia. Elderly individuals outperformed schizophrenia patients in the adaptation learning tasks.

Unprecedented heavy rainfall event over Yamunanagar, India during 14 July 2016: An observational and modelling study

AbstractExtreme rainfall events have posed several serious threats to many populated and urbanized areas in the world including the Indian subcontinent. Therefore, accurate predictions of their intensity and areas of influence are important for flood‐prone risk assessments. On 14 July 2016, heavy to exceptionally heavy rainfall occurred in Yamunanagar (30.16° N, 77.29° E), located in the state of Haryana in North India, which led to widespread disruption of communication, electricity, inundation of houses, and so forth. The present paper aims at examining observational, synoptic, thermo‐dynamical, and numerical features associated with this devastating rainfall episode. The analysis found that during extreme rainfall episodes, a trough in mid‐tropospheric westerlies and a strong low‐level atmospheric monsoonal flow seem to have strongly interacted with each other, creating a strong convergence zone near study areas that led to a severe rainstorm. The quasi‐stationary supercells were also noticed due to continuous moisture incursions from the Bay of Bengal and orographic uplift over the Himalayas near Yamunanagar. A deep layer of wind shear interacts dynamically with the convergence zone and leads to a potential rainstorm. Thermodynamic indices indicate high instability over the heavy rainfall area. The dynamics of this event were studied in detail by using three‐dimensional variational data assimilation within the weather research and forecasting model, configured with triple two‐way nesting domains (27, 9, and 3 km). The model results show that the weather research and forecasting model satisfactorily captures the quantitative precipitation (300 mm) in 24 h over the Yamunanagar region as compared with observation (365 mm).

Disruption of Glucocorticoid Action on CD11c+ Dendritic Cells Favors the Generation of CD4+ Regulatory T Cells and Improves Fetal Development in Mice.

A wealth of innate and adaptive immune cells and hormones are involved in mounting tolerance towards the fetus, a key aspect of successful reproduction. We could recently show that the specific cross talk between the pregnancy hormone progesterone and dendritic cells (DCs) is significantly engaged in the generation of CD4+ FoxP3+ regulatory T (Treg) cells while a disruption led to placental alterations and intra-uterine growth restriction. Apart from progesterone, also glucocorticoids affect immune cell functions. However, their functional relevance in the context of pregnancy still needs clarification. We developed a mouse line with a selective knockout of the glucocorticoid receptor (GR) on DCs, utilizing the cre/flox system. Reproductive outcome and maternal immune and endocrine adaptation of Balb/c-mated C57Bl/6 GRflox/floxCD11ccre/wt (mutant) females was assessed on gestation days (gd) 13.5 and 18.5. Balb/c-mated C57Bl/6 GRwt/wtCD11ccre/wt (wt) females served as controls. The number of implantation and fetal loss rate did not differ between groups. However, we identified a significant increase in fetal weight in fetuses from mutant dams. While the frequencies of CD11c+ cells remained largely similar, a decreased expression of co-stimulatory molecules was observed on DCs of mutant females on gd 13.5, along with higher frequencies of CD4+ and CD8+ Treg cells. Histomorphological and gene expression analysis revealed an increased placental volume and an improved functional placental capacity in mice lacking the GR on CD11c+ DCs. In summary, we here demonstrate that the disrupted communication between GCs and DCs favors a tolerant immune microenvironment and improves placental function and fetal development.

Bacillus spp. Inhibit Edwardsiella tarda Quorum-Sensing and Fish Infection.

The disruption of pathogen communication or quorum-sensing (QS) via quorum-quenching (QQ) molecules has been proposed as a promising strategy to fight bacterial infections. Bacillus spp. have recognizable biotechnology applications, namely as probiotic health-promoting agents or as a source of natural antimicrobial molecules, including QQ molecules. This study characterized the QQ potential of 200 Bacillus spp., isolated from the gut of different aquaculture fish species, to suppress fish pathogens QS. Approximately 12% of the tested Bacillus spp. fish isolates (FI). were able to interfere with synthetic QS molecules. Ten isolates were further selected as producers of extracellular QQ-molecules and their QQ capacity was evaluated against the QS of important aquaculture bacterial pathogens, namely Aeromonas spp., Vibrio spp., Photobacterium damselae, Edwardsiela tarda, and Shigella sonnei. The results revealed that A. veronii and E. tarda produce QS molecules that are detectable by the Chr. violaceum biosensor, and which were degraded when exposed to the extracellular extracts of three FI isolates. Moreover, the same isolates, identified as B. subtilis, B. vezelensis, and B. pumilus, significantly reduced the pathogenicity of E. tarda in zebrafish larvae, increasing its survival by 50%. Taken together, these results identified three Bacillus spp. capable of extracellularly quenching aquaculture pathogen communication, and thus become a promising source of bioactive molecules for use in the biocontrol of aquaculture bacterial diseases.

COVID-19 and Aphasia.

Purpose of Review Aphasia is an acquired neurological language disorder after brain damages. Persons with aphasia (PWA) are more susceptible to behavioral and emotional implications due to inherent communication and/or cognitive difficulties. Currently, little is known regarding the impact of COVID-19 on PWA.Recent FindingsThere are now growing reports with evidence of neurological and dysexecutive syndromes subsequent to interference of brain functions in acute patients with COVID-19, leading to variable aphasia-like symptoms. COVID-19 affected chronic PWA more in terms of disrupted communication and daily routines, worsened psychosocial well-being, and difficulties getting aphasia services that adequately addressed their needs.SummaryAcute versus chronic PWA were disproportionately affected by COVID-19. Recognizing, examining, and managing COVID-19-related neurological and behavioral problems in PWA is not straightforward. As we passed the 1-year mark and approaching the 2-year mark of the onset of COVID-19, more research is necessary to prioritize strategies for improving current evidence-based care and rehabilitation of aphasia.

Oxyphyllanene B overcomes temozolomide resistance in glioblastoma: Structure–activity relationship and mitochondria-associated ER membrane dysfunction

The identification of novel therapeutic candidates from natural products for the development of chemoresistant glioblastoma multiforme (GBM) treatment has been a highly significant and effective strategy. Sesquiterpenes are a class of naturally occurring 15-carbon isoprenoid compounds, and several types of sesquiterpenes have the ability to induce growth inhibition and apoptosis in a variety of cancer cell lines. In the present study, 56 sesquiterpenes of five types, namely, eudesmane-type (I) (1-24), eremophilane-type (II) (25-32), cadinane-type (III) (33-41), guaiane-type (IV) (42-49), and oplopanone-type (V) (50-56), were screened for their antiglioma activity, structure-activity relationship analysis (SAR), and underlying mechanism based on patient-derived recurrent GBM strains, patient-derived GBM cell sphere, GBM organoid (GBO) models, and temozolomide (TMZ)-resistant GBM cell lines. We found that compound 12 (oxyphyllanene B, OLB) showed the most potent antiglioma activity, and we confirmed that OLB could induce apoptosis in a time- and dose-dependent manner in TMZ-resistant GBM cells and GBOs. SAR announced that the presence of an α, β-unsaturated carbonyl moiety was likely to enhance cytotoxic activities. Mechanistic studies demonstrated that OLB induced abnormal changes in ER and mitochondria-associated membrane (MAM) networks, which triggered ER stress, mitochondrial dysfunction, and apoptosis. Furthermore, our findings suggested that OLB-triggered PACS2 activation might form a committed step to disrupt ER-mitochondria communication and showed for the first time that the expression levels of PACS2 might positively correlate with the progression and chemotherapy resistance of glioma. Our results indicated that OLB might be a promising candidate for treating TMZ-resistant GBM cells by activating PACS2, which triggered a crucial event to promote the disruption of ER-mitochondria communication and overcome chemotherapy resistance of GBM.

Pheromone-Mediated Mating Disruption as Management Option for Cydia spp. in Chestnut Orchard.

Simple SummaryInvestigations were conducted to evaluate the effectiveness of mating disruption (MD) to control chestnut tortrix moths. Surveys were performed at four sites in northern Italy in 2019–2020. MD was carried out using the pheromone dispenser Ecodian® CT. The total number of trapped males was significantly lower in MD plots than in control ones for all sites and years. Trap catch suppression in MD plots averaged 89.5% and 93.8% for Cydia fagiglandana and 57.4% and 81% for Cydia splendana in 2019 and 2020, respectively. A reduction of about 71% of larval infestation of chestnut fruits was only recorded in the MD plot at one site in 2019. Although the reduction in male catches in MD plots was observed, this research highlighted the need to perform further studies to investigate the effect of MD in reducing larval damage of chestnut fruits.(1) Background: Pheromone-based devices are successfully used to control insect pests in agriculture. (2) Methods: Investigations were conducted to evaluate the effectiveness of mating disruption (MD) to control the chestnut tortrix moths, Cydia fagiglandana and Cydia splendana. Surveys were performed in northern Italy in 2019–2020. MD was carried out using the pheromone dispenser Ecodian® CT. The effectiveness of MD was assessed by recording male adult catches in pheromone-baited sticky traps and larvae in chestnut fruits, comparing MD and control plots. (3) Results: The total number of trapped males was significantly lower in MD plots than in control ones, for all sites and years. Trap catch suppression in MD plots averaged 89.5% and 93.8% for C. fagiglandana and 57.4% and 81% for C. splendana in 2019 and 2020, respectively. The larval infestation rate in fruits did not vary between plots except for one site where a reduction of about 71% in the MD plot was recorded in 2019. (4) Conclusions: Low catches in MD plots turned out to be a good measure of the effectiveness of communication disruption, but no satisfactory data have been obtained regarding fruit infestation, highlighting how the reduction of male catches cannot always be considered as a reliable indicator of successful control. Specific investigations about background population density, dispersal and mating/oviposition behavior are thus essential for a viable management strategy.