Wildlife Disease Research Articles

Important Mycoses of Wildlife: Emphasis on Etiology, Epidemiology, Diagnosis, and Pathology-A Review: PART 1.

Simple SummaryThe number of wild animals is steadily declining globally, so the early diagnosis and proper treatment of emerging diseases are vital. Fungal diseases are commonly encountered in practice and have a high zoonotic potential. This article describes aspergillosis, candidiasis, histoplasmosis, cryptococcosis, and penicilliosis, and is only the first part of a detailed review. The laboratory methods (fungal isolation, gross pathology, histopathology, histochemistry, cytology, immunohistochemistry, radiography, CT, PCR, or ELISA) used in the diagnosis and the clinical details that provide a complete view of the mycoses are presented.In the past few years, there has been a spurred tripling in the figures of fungal diseases leading to one of the most alarming rates of extinction ever reported in wild species. Some of these fungal diseases are capable of virulent infections and are now considered emerging diseases due to the extremely high number of cases diagnosed with fungal infections in the last few decades. Most of these mycotic diseases in wildlife are zoonotic, and with the emergence and re-emergence of viral and bacterial zoonotic diseases originating from wildlife, which are causing devastating effects on the human population, it is important to pay attention to these wildlife-borne mycotic diseases with zoonotic capabilities. Several diagnostic techniques such as fungal isolation, gross pathology, histopathology, histochemistry, cytology, immunohistochemistry, radiography, CT, and molecular methods such as PCR or ELISA have been invaluable in the diagnosis of wildlife mycoses. The most important data used in the diagnosis of these wildlife mycoses with a zoonotic potential have been re-emphasized. This will have implications for forestalling future epidemics of these potential zoonotic mycotic diseases originating from wildlife. In conclusion, this review will highlight the etiology, epidemiology, diagnosis, pathogenesis, pathogenicity, pathology, and hematological/serum biochemical findings of five important mycoses found in wild animals.

Strategies for managing marine disease.

The incidence of emerging infectious diseases (EIDs) has increased in wildlife populations in recent years and is expected to continue to increase with global environmental change. Marine diseases are relatively understudied compared with terrestrial diseases but warrant parallel attention as they can disrupt ecosystems, cause economic loss, and threaten human livelihoods. Although there are many existing tools to combat the direct and indirect consequences of EIDs, these management strategies are often insufficient or ineffective in marine habitats compared with their terrestrial counterparts, often due to fundamental differences between marine and terrestrial systems. Here, we first illustrate how the marine environment and marine organism life histories present challenges and opportunities for wildlife disease management. We then assess the application of common disease management strategies to marine versus terrestrial systems to identify those that may be most effective for marine disease outbreak prevention, response, and recovery. Finally, we recommend multiple actions that will enable more successful management of marine wildlife disease emergencies in the future. These include prioritizing marine disease research and understanding its links to climate change, improving marine ecosystem health, forming better monitoring and response networks, developing marine veterinary medicine programs, and enacting policy that addresses marine and other wildlife diseases. Overall, we encourage a more proactive rather than reactive approach to marine wildlife disease management and emphasize that multidisciplinary collaborations are crucial to managing marine wildlife health.

How do the practical and pragmatic limitations in the design or implementation of wildlife disease surveillance systems bias our understanding of the drivers, epidemiology, and impact of pathogen traffic between wildlife and people or domestic species, or within wildlife host populations?

Decision makers want more information on the presence, distribution, movement, and impacts of pathogens in wild animals to anticipate or assess the risk of pathogen spillover from wildlife to people or domestic animals or to better understand the conservation implications of contagious diseases in wildlife. There are many impediments to designing and implementing a wildlife health surveillance system that fulfils all the expectations for ‘good’ surveillance. There are well-known pragmatic limitations such as the inability to sample targeted populations consistently and regularly in a representative manner, lack of validated tests, and funding limitations that restrict surveillance to periodic surveys. The nature and impact of the biases on data collection, generation, management, quality, analysis, interpretations, and information sharing are poorly studied. We invite authors to address the question in the title.

Understanding and strengthening wildlife and zoonotic disease policy processes: A research imperative.

The COVID-19 pandemic highlights the urgency and importance of monitoring, managing and addressing zoonotic diseases, and the acute challenges of doing so with sufficient inter-jurisdictional coordination in a dynamic global context. Although wildlife pathogens are well-studied clinically and ecologically, there is very little systematic scholarship on their management or on policy implications. The current global pandemic therefore presents a unique social science research imperative: to understand how decisions are made about preventing and responding to wildlife diseases, especially zoonoses, and how those policy processes can be improved as part of early warning systems, preparedness and rapid response. To meet these challenges, we recommend intensified research efforts towards: (i) generating functional insights about wildlife and zoonotic disease policy processes, (ii) enabling social and organizational learning to mobilize those insights, (iii) understanding epistemic instability to address populist anti-science and (iv) anticipating evolving and new zoonotic emergences, especially their human dimensions. Since policy processes for zoonoses can be acutely challenged during the early stages of an epidemic or pandemic, such insights can provide a pragmatic, empirically-based roadmap for enhancing their robustness and efficacy, and benefiting long-term decision-making efforts.

Amplification in Time and Dilution in Space: Partitioning Spatiotemporal Processes to Assess the Role of Avian-Host Phylodiversity in Shaping Eastern Equine Encephalitis Virus Distribution

Eastern equine encephalitis virus (EEEv) is an arthropod-borne virus and the causative agent of neurologic disease in humans, horses, poultry, and wildlife. Although EEEv is known to be transmitted in cycles involving avian hosts and ornithophilic mosquitoes, there is ongoing debate about the role avian-host phylodiversity plays in diluting or amplifying virus prevalence across geographic space and through time. This study leveraged seventeen years of non-human EEEv detections to quantify possible EEEv dilution and amplification effects in response to avian-host phylodiversity. In assessing EEEv and avian-host diversity relationships, comparisons were performed to illustrate how modeling decisions aimed at capturing spatial patterns, temporal trends, and space–time interactions impacted results and the interpretations drawn from those results. Principal findings indicated that increased avian phylodiversity promotes EEEv dilution across geographic space, but this dilution effect is scale-dependent and masked by amplification effects that occur through time. Findings further demonstrated that the decisions made when modeling complex spatiotemporal dynamics can readily contribute to contrasting statistical outcomes and results misinterpretation, even when arithmetic and mathematics are strictly correct.

Novel chytrid pathogen variants and the global amphibian pet trade.

Global wildlife trade spreads emerging infectious diseases that threaten biodiversity. The amphibian chytrid pathogen Batrachochytrium dendrobatidis (Bd) has caused population declines and species extinctions worldwide except in Asia. Fire-bellied toads (Bombina orientalis), exported in large numbers from Asia, are tolerant of Bd and carry hypervirulent ancestral chytrid BdAsia-1 variants. We assayed the virulence of a new isolate of BdAsia-1 on the model Australasian frog host Litoria caerulea. Infected individuals (n = 15) all showed rapid disease progression culminating in death, whereas sham-inoculated individuals (n = 10) presented no clinical signs of disease and all survived (log rank test, χ2 = 15.6, df = 1, p < 0.0001). The virulence of the new isolate of BdAsia-1 is comparable tothe one we assayed previously (χ2 = 0.0, df = 1, p = 0.91). Internationally traded wildlife, even when they appear healthy, can carry hypervirulent variants of pathogens. Once new pathogen variants escape into the environment, native species that have had no opportunity to evolve resistance to them may perish. Our study suggests that hypervirulent pathogens are being spread by the international pet trade. Notifiable wildlife diseases attributable to locally endemic pathogens often fail to generate conservation concern so are rarely subject to border surveillance or import controls. Because of the danger novel variants pose, national border control agencies need to implement disease screening and quarantine protocols to ensure the safety of their endemic fauna.

A Review of Non-Invasive Sampling in Wildlife Disease and Health Research: What's New?

Simple SummaryThe interest in wildlife research has increased in the last decades as more scientists work within a One Health framework that regards human, livestock and wildlife health as connected entities. To minimise the impact of research on wildlife, collecting samples with as little disturbance of the animals as possible is important. In our review, we assess the use of so-called non-invasive sampling and summarise which samples can be used successfully when carrying out research on wildlife diseases and health status. Our results show that interest in minimally invasive sampling has steadily increased since the 2010s. Topics able to employ these methods include disease research, but also stress and other hormone assessments, pollution studies, and dietary studies. At the moment, such methods are mainly used to collect samples from land mammals, however, they can also be used in a wide range of other animals. Ever more capable analytical methods will allow for an even wider use of such “animal-friendly” sampling methods.In the last decades, wildlife diseases and the health status of animal populations have gained increasing attention from the scientific community as part of a One Health framework. Furthermore, the need for non-invasive sampling methods with a minimal impact on wildlife has become paramount in complying with modern ethical standards and regulations, and to collect high-quality and unbiased data. We analysed the publication trends on non-invasive sampling in wildlife health and disease research and offer a comprehensive review on the different samples that can be collected non-invasively. We retrieved 272 articles spanning from 1998 to 2021, with a rapid increase in number from 2010. Thirty-nine percent of the papers were focussed on diseases, 58% on other health-related topics, and 3% on both. Stress and other physiological parameters were the most addressed research topics, followed by viruses, helminths, and bacterial infections. Terrestrial mammals accounted for 75% of all publications, and faeces were the most widely used sample. Our review of the sampling materials and collection methods highlights that, although the use of some types of samples for specific applications is now consolidated, others are perhaps still underutilised and new technologies may offer future opportunities for an even wider use of non-invasively collected samples.

The efficacy of wildlife fences for keeping reindeer outside a chronic wasting disease risk area

Abstract Emerging wildlife diseases often comes with negative cultural and economic impact. Limiting disease spread is a recurrent goal and challenge, but the efficacy of various mitigation measures is rarely assessed. Chronic wasting disease (CWD) is a lethal disease among cervids that was discovered among alpine reindeer (Rangifer tarandus) in the Nordfjella mountain range of Norway in 2016. After de‐population, the entire range was fallowed to avoid re‐emergence from environmental pathogen reservoirs. This involved installing perimeter fences in the alpine areas in order to keep reindeer from adjacent populations outside of the CWD risk area, while other cervids (red deer Cervus elaphus, roe deer Capreolus capreolus and moose Alces alces) were likely to enter through the forested areas. We used camera trapping and surveillance reports to assess the efficacy of the perimeter fences. All four species of cervids were documented inside the CWD risk area. For reindeer, only 12.0% of observations were inside the CWD risk area, while this was 28.7% for the other cervids. The higher proportion of observations outside of the fenced area indicate that fences provided a barrier and lowered the number of crossings also of red deer, roe deer and moose. Fences do not provide complete barriers, and we discuss practical solutions for how to avoid ‘intruders’ entering a given area, such as maintenance at critical points (e.g. river and road crossings) and height of fences (e.g. species variation in jumping; deep snow) to uphold their desired effect. We argue that two fence lines with a buffer zone would be required when re‐introduction of reindeer are planned in the CWD risk area after fallowing, similar to what has been suggested for other wildlife diseases.

Bighorn sheep show similar in-host responses to the same pathogen strain in two contrasting environments.

Ecological context—the biotic and abiotic environment, along with its influence on population mixing dynamics and individual susceptibility—is thought to have major bearing on epidemic outcomes. However, direct comparisons of wildlife disease events in contrasting ecological contexts are often confounded by concurrent differences in host genetics, exposure histories, or pathogen strains. Here, we compare disease dynamics of a Mycoplasma ovipneumoniae spillover event that affected bighorn sheep populations in two contrasting ecological contexts. One event occurred on the herd's home range near the Rio Grande Gorge in New Mexico, while the other occurred in a captive facility at Hardware Ranch in Utah. While data collection regimens varied, general patterns of antibody signal strength and symptom emergence were conserved between the two sites. Symptoms appeared in the captive setting an average of 12.9 days postexposure, average time to seroconversion was 24.9 days, and clinical signs peaked at approximately 36 days postinfection. These patterns were consistent with serological testing and subsequent declines in symptom intensity in the free‐ranging herd. At the captive site, older animals exhibited more severe declines in body condition and loin thickness, higher symptom burdens, and slower antibody response to the pathogen than younger animals. Younger animals were more likely than older animals to clear infection by the time of sampling at both sites. The patterns presented here suggest that environment may not be a major determinant of epidemiological outcomes in the bighorn sheep—M. ovipneumoniae system, elevating the possibility that host‐ or pathogen‐factors may be responsible for observed variation.

Potential Facilitation Between a Commensal and a Pathogenic Microbe in a Wildlife Disease.

We assessed the potential for microbial interactions influencing a well-documented host-pathogen system. Mycoplasma agassizii is the known etiological agent of upper respiratory tract disease in Mojave desert tortoises (Gopherus agassizii), but disease in wild animals is extremely heterogeneous. For example, a much larger proportion of animals harbor M. agassizii than those that develop disease. With the availability of a new quantitative PCR assay for a microbe that had previously been implicated in disease, Pasteurella testudinis, we tested 389 previously collected samples of nasal microbes from tortoise populations across the Mojave desert. We showed that P. testudinis is a common commensal microbe. However, we did find that its presence was associated with higher levels of M. agassizii among the tortoises positive for this pathogen. The best predictor of P. testudinis prevalence in tortoise populations was average size of tortoises, suggesting that older populations have higher levels of P. testudinis. The prevalence of co-infection in populations was associated with the prevalence of URTD, providing additional evidence for an indirect interaction between the two microbes and inflammatory disease. We showed that URTD, like many chronic, polymicrobial diseases involving mucosal surfaces, shows patterns of a polymicrobial etiology.

Habitat availability alters the relative risk of a bovine tuberculosis breakdown in the aftermath of a commercial forest clearfell disturbance

Abstract Human modification of landscapes and associated disturbances may facilitate the emergence and spread of zoonotic diseases. Policy‐makers need better understanding of the link between anthropogenic disturbances and wildlife disease hosts at the interface of human society and the natural environment, for example agriculture, forestry and aquaculture. Empirical research is strongly needed for the control of novel zoonoses which might emerge, as well as the management of existing zoonoses with significant economic repercussions such as bovine tuberculosis (bTB). We aimed to examine the link between ecological disturbance and relative bTB risk using Ireland as a case study. We analysed clearfell forestry operations and assessed bTB breakdowns within cattle farms across different spatio‐temporal scales over multiple years, examining how ecological conditions may modulate this relationship using conditional logistic regression models. We found a significant effect of the interaction between the extent of clearfell forestry removed and the extent of natural grassland and mixed forestry present on relative bTB risk. This interaction was dynamic, leading to an increase or decrease of the relative bTB risk depending on where (between 2 and 6 km from the farm) and when (between 0 and 36 months prior to the bTB outbreak) the clearfell operations occurred. Our study provides empirical evidence of the link between mechanised forestry operations and fluctuating relative bTB risk in cattle farms, although the mechanism behind it is yet to be elucidated. Given our data, we hypothesise that wildlife hosts may abandon the area subjected to clearfell when disturbance is highest (during active operations and shortly afterward) but are subsequently attracted back to the site as they regenerate, potentially affecting the contact rates with livestock and thus, relative bTB risk. Synthesis and applications. Our analysis demonstrates that landscape modification is correlated with a change in relative bovine tuberculosis (bTB) risk that is dynamic in time and space. Understanding that the effect of landscape change is transient allows managers to understand the risk for neighbouring farms and inform policy accordingly. Landscape‐level studies are necessary to unveil subtle‐ecological processes, shifting research and management efforts away from cattle herd‐centric policy and toward macroecological surveillance of wildlife hosts and longitudinal assessment of bTB risk.

The predicted impact of resource provisioning on the epidemiological responses of different parasites.

Anthropogenic activities and natural events such as periodic tree masting can alter resource provisioning in the environment, directly affecting animals, and potentially impacting the spread of infectious diseases in wildlife. The impact of these additional resources on infectious diseases can manifest through different pathways, affecting host susceptibility, contact rate and host demography.To date however, empirical research has tended to examine these different pathways in isolation, for example by quantifying the effects of provisioning on host behaviour in the wild or changes in immune responses in controlled laboratory studies. Furthermore, while theory has investigated the interactions between these pathways, this work has focussed on a narrow subset of pathogen types, typically directly transmitted microparasites. Given the diverse ways that provisioning can affect host susceptibility, contact patterns or host demography, we may expect the epidemiological consequences of provisioning to vary among different parasite types, dependent on key aspects of parasite life history, such as the duration of infection and transmission mode.Focusing on an exemplar empirical system, the wood mouse Apodemus sylvaticus, and its diverse parasite community, we developed a suite of epidemiological models to compare how resource provisioning alters responses for a range of these parasites that vary in their biology (microparasite and macroparasite), transmission mode (direct, environmental and vector transmitted) and duration of infection (acute, latent and chronic) within the same host population.We show there are common epidemiological responses to host resource provisioning across all parasite types examined. In particular, the epidemiological impact of provisioning could be driven in opposite directions, depending on which host pathways (contact rate, susceptibility or host demography) are most altered by the addition of resources to the environment. Broadly, these responses were qualitatively consistent across all parasite types, emphasising the importance of identifying general trade‐offs between provisioning‐altered parameters.Despite the qualitative consistency in responses to provisioning across parasite types, we predicted notable quantitative differences between parasites, with directly transmitted parasites (those conforming to SIR and SIS frameworks) predicted to show the strongest responses to provisioning among those examined, whereas the vector‐borne parasites showed negligible responses to provisioning. As such, these analyses suggest that different parasites may show different scales of response to the same provisioning scenario, even within the same host population. This highlights the importance of knowing key aspects of host–parasite biology, to understand and predict epidemiological responses to provisioning for any specific host–parasite system.

Tourism may threaten wildlife disease refugia

AbstractThe remoteness and isolation of South American tabletop mountain (tepui) summits may protect against infections that underpin global amphibian declines. Increases in recreational pressure in such unspoiled destinations, and in isolated ecosystems globally, pose a poorly understood risk of wildlife disease introduction, especially in supposedly immunologically naïve communities. We here report the first observed chytrid infections in the Pantepui biogeographical region. Infections significantly correlate with proximity to the nearest basic tourist infrastructure in four endemic amphibians occurring on tepui summits and their slopes. Phylogenetic relationships and environmental context suggest a high risk of severe and irreversible population declines in unique, early branching amphibians. These findings advocate for an urgent control of recreational pressure in isolated, highly vulnerable ecosystems.

Listeriosis with viral coinfections in 8 gray foxes, 8 wild turkeys, and 2 young cervids in the southeastern United States.

Listeria monocytogenes is a bacterium that can cause disease in many species, including humans, livestock, and wildlife. Increased interactions via shared habitats may promote pathogen transmission among these groups. Our objectives were to evaluate the Southeastern Cooperative Wildlife Disease Study diagnostic data to characterize and compare L. monocytogenes-induced lesions and comorbidities in gray foxes and wild turkeys, and to describe cases of listeriosis in 2 cervids. From 1991-2020, 8 gray foxes, 8 wild turkeys, a neonatal elk, and a white-tailed deer fawn from several eastern states in the United States were diagnosed with listeriosis. All 8 foxes had hepatitis and/or hepatic necrosis with intralesional gram-positive bacilli, and concurrent canine distemper virus (CDV) infection; 2 of the foxes had been vaccinated recently for CDV. L. monocytogenes was cultured from the liver (6 of 8) or lung (2 of 8) of foxes. Lesions in wild turkeys included hepatocellular necrosis (3 of 8), heterophilic hepatitis (1 of 8), heterophilic granulomas (1 of 8), intrasinusoidal gram-positive bacilli without hepatic lesions (1 of 8), granulomatous dermatitis (1 of 8), and/or granulomatous myocarditis (2 of 8). Lymphoproliferative disease viral DNA was detected in 5 of 6 turkeys tested; reticuloendotheliosis viral DNA was detected in 2 of 3 turkeys tested. Both cervids had systemic listeriosis, with L. monocytogenes isolated from liver. Immunohistochemistry for Listeria spp. on select cases revealed immunolabeling in affected organs. Listeriosis was thus established as a cause of morbidity and mortality in 3 wildlife species, which often suffered from concurrent infections and likely immunosuppression.

Point Prevalence Studies Are Necessary as First Steps in Studying the Epidemiology of Viruses and Other Pathogens Transmitted by Arthropods.

Studies of the epidemiology of arthropod-borne viruses are based on understanding whether a given virus is found in a particular location or locations, how prevalent the virus is in that area, which vertebrate hosts serve as reservoirs of the virus, the relationship between the size of the populations of these reservoirs and the prevalence of the virus and, of course, whether the virus causes human, livestock, or wildlife diseases, as well as other characteristics. However, seasonal conditions fluctuate, annual conditions fluctuate, human impacts alter the environment, and the habitat and ecosystems naturally change. Because these parameters provide a more encompassing view of the natural history of a virus, it is important to recognize that point prevalence studies comprise only a single view of the situation and that longitudinal studies are required to obtain a more complete and useful understanding of the natural history of the virus. This paper provides details as to whether and how to conduct such studies.

Discovery of a Novel Coronavirus in Swedish Bank Voles (Myodes glareolus).

The unprecedented pandemic COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), with bats as original reservoirs, has once again highlighted the importance of exploring the interface of wildlife diseases and human health. In this study, we identified a novel Betacoronavirus from bank voles (Myodes glareolus) in Grimsö, Sweden, and this virus is designated as Grimso virus. Repeated detection over three years and an overall prevalence of 3.4% suggest that the virus commonly occurs in bank voles. Furthermore, phylogenetic analyses indicate that the Grimso virus belongs to a highly divergent Embecovirus lineage predominantly associated with bank voles. Given that bank voles are one of the most common rodent species in Sweden and Europe, our findings indicate that Grimso virus might be circulating widely in bank voles and further point out the importance of sentinel surveillance of coronaviruses in wild small mammalian animals, especially in wild rodents.

When Defenses Fail: Atelopus zeteki Skin Secretions Increase Growth of the Pathogen Batrachochytrium dendrobatidis.

To combat the threat of emerging infectious diseases in wildlife, ecoimmunologists seek to understand the complex interactions among pathogens, their hosts, and their shared environments. The cutaneous fungal pathogen Batrachochytrium dendrobatidis (Bd), has led to the decline of innumerable amphibian species, including the Panamanian golden frog (Atelopus zeteki). Given that Bd can evade or dampen the acquired immune responses of some amphibians, nonspecific immune defenses are thought to be especially important for amphibian defenses against Bd. In particular, skin secretions constitute a vital component of amphibian innate immunity against skin infections, but their role in protecting A. zeteki from Bd is unknown. We investigated the importance of this innate immune component by reducing the skin secretions from A. zeteki and evaluating their effectiveness against Bd in vitro and in vivo. Following exposure to Bd in a controlled inoculation experiment, we compared key disease characteristics (e.g., changes in body condition, prevalence, pathogen loads, and survival) among groups of frogs that had their skin secretions reduced and control frogs that maintained their skin secretions. Surprisingly, we found that the skin secretions collected from A. zeteki increased Bd growth in vitro. This finding was further supported by infection and survival patterns in the in vivo experiment where frogs with reduced skin secretions tended to have lower pathogen loads and survive longer compared to frogs that maintained their secretions. These results suggest that the skin secretions of A. zeteki are not only ineffective at inhibiting Bd but may enhance Bd growth, possibly leading to greater severity of disease and higher mortality in this highly vulnerable species. These results differ from those of previous studies in other amphibian host species that suggest that skin secretions are a key defense in protecting amphibians from developing severe chytridiomycosis. Therefore, we suggest that the importance of immune components cannot be generalized across all amphibian species or over time. Moreover, the finding that skin secretions may be enhancing Bd growth emphasizes the importance of investigating these immune components in detail, especially for species that are a conservation priority.

Metabarcoding Approaches in Amphibian Disease Ecology: Disentangling the Functional Contributions of Skin Bacteria on Disease Outcome.

Molecular technologies have revolutionized the field of wildlife disease ecology, allowing the detection of outbreaks, novel pathogens, and invasive strains. In particular, metabarcoding approaches, defined here as tools used to amplify and sequence universal barcodes from a single sample (e.g., 16S rRNA for bacteria, ITS for fungi, 18S rRNA for eukaryotes), are expanding our traditional view of host-pathogen dynamics by integrating microbial interactions that modulate disease outcome. Here, I provide an analysis from the perspective of the field of amphibian disease ecology, where the emergence of multi-host pathogens has caused global declines and species extinctions. I reanalyzed an experimental mesocosm dataset to infer the functional profiles of the skin microbiomes of coqui frogs (Eleutherodactylus coqui), an amphibian species that is consistently found infected with the fungal pathogen Batrachochytrium dendrobatidis and has high turnover of skin bacteria driven by seasonal shifts. I found that the metabolic activities of microbiomes operate at different capacities depending on the season. Global enrichment of predicted functions was more prominent during the warm-wet season, indicating that microbiomes during the cool-dry season were either depauperate, resistant to new bacterial colonization, or that their functional space was more saturated. These findings suggest important avenues to investigate how microbes regulate population growth and contribute to host physiological processes. Overall, this study highlights the current challenges and future opportunities in the application of metabarcoding to investigate the causes and consequences of disease in wild systems.

Residents’ concerns and attitudes regarding wildlife disease management: A case of chronic wasting disease in Tennessee

ABSTRACT Most of the literature on the chronic wasting disease (CWD) has focused on the attitudes of deer hunters. Designing public policies to manage CWD requires an understanding of how other stakeholders (landowners, non-hunting public) perceive the risk and react. By surveying a sample of residents in Tennessee, this study compared the concerns, agency trust, and acceptability of management actions among resident groups segmented based on their proximity to CWD presence, hunter status, and land ownership status. Responses from seven CWD counties and four adjacent counties were generally similar, but notable differences were found between hunters and non-hunters and between landowners and non-landowners. Considering that the cooperation from all stakeholders will be critical for the success of a regional CWD management program, it may be important for wildlife agencies to recognize the heterogeneity in preferences and the sensitivity of proposed actions among stakeholders before implementation and enforcement.

Enhancing inter-organizational collaboration for wildlife disease surveillance in Sri Lanka.

Management of zoonotic infectious diseases is an urgent global heath imperative. Interdisciplinary approaches for zoonosis management exist in literature, but collaboratively implementing them is a pervasive challenge. The Sri Lanka Wildlife Health Centre (SLWHC) was created in 2011 to coordinate wildlife disease surveillance and response among government agencies. We interviewed SLWHC-affiliated personnel about existing communication and collaboration channels to identify operational needs as well as potential enhancements for the SLWHC's operations. We used the Policy Sciences' analytical framework to identify opportunities and challenges for the SLWHC. Study participants held both human and animal health as the utmost priorities. However, their observations indicate that inter-organizational communication barriers and intra-organizational hierarchies still need to be overcome for the Centre's partnering organizations to collaborate to their fullest potential. Any interventions to enhance the SLWHC's collaborative capacity for detecting and managing zoonotic disease outbreaks could be strengthened by appealing to participants' shared value orientations towards enlightenment and respect. A common interest was the desire to collaborate and combine resources, knowledge and personnel to detect, reduce and prevent the incidence of zoonotic disease outbreaks in Sri Lanka. These lessons about institutionalizing communication have considerable relevance for organizational responses to the current SARS-CoV2 pandemic and other zoonoses.