Different Sources Of Starch Research Articles

Pig diets formulated with different sources of starch based in vitro kinetics of starch digestion

Pig diets formulated with different sources of starch based in vitro kinetics of starch digestion

Preparation and properties of phosphate starches from tuberous roots

The diversification of raw materials in the starch industries is a current strategy. However, the production of native starches does not meet market demand, and it is essential to expand the knowledge about chemical modifications in the same production line for different sources of starch. Phosphate starches are one of the most abundantly produced and widely used chemically modified starches. However, the effects of this modification may vary with the starch source and the reaction conditions. In this study, arrowroot, cassava and sweet potato starches were modified with sodium trimetaphosphate (STMP)/sodium tripolyphosphate (STPP) mixture under same conditions. The reaction time ranged from 7.5 to 120 min. Unmodified and modified starches were analyzed for phosphorus, amylose, morphology, X-ray diffraction pattern, crystallinity, swelling power, solubility, pasting and thermal properties. Phosphorus content linked to the starches increased with the reaction time, which affected the physicochemical properties of the three starches. The changes were more significant in all reaction times for cassava starch, followed by arrowroot. Due to its intrinsic characteristics, longer reaction times were necessary for more significant changes in sweet potato starch. Regardless of the starch source, as the reaction time increased, the average starch granule diameter, swelling power, solubility and peak viscosity increased. There was a decrease in setback in the longer reaction times for cassava and arrowroot starches. The changes in the reaction times allowed obtaining phosphate tuberous starches with different properties which can meet the demands of the food and non-food industries.

Functional properties of starch-chitosan blend bionanocomposite films for food packaging: the influence of amylose-amylopectin ratios.

The present study is focused on the influence of amylose and amylopectin ratio on crystallinity, water barrier, mechanical, morphological and anti-fungal properties of starch-based bionanocomposite films. The different sources of starch containing various proportion of amylose and amylopectin (high amylose corn starch, 70:30; corn starch, 28:72; wheat starch, 25:75; and potato starch, 20:80) has been incorporated with chitosan (CH) and nanoclay (Na-MMT). Amylose and amylopectin ratio has regulated the orientation of molecular structure in the starch-based films. Experimental results have revealed that the prepared bionanocomposite films that of CS/CH/nanoclay has exhibited higher crystallinity and molecular miscibility among corn starch, with chitosan and nanoclay were confirmed by XRD. CS/CH/nanoclay has exhibited lowest water vapor permeability and highest tensile strength due to molecular space present in corn starch. Fourier transform infrared spectroscopy has confirmed the shift of amine peak to a higher wavenumber indicating a stronger hydrogen bond between starch and chitosan. Finally, the best bionanocomposite films were tested for food packaging applications. Low-density polyethylene has exhibited fungal growth on 5th day when packed with bread slices at 25°C and 59% RH whereas CS/CH/nanoclay bionanocomposite film did not show the same for at least 20days. CS/CH/nanoclay film could potentially be useful for active packaging in extending shelf life; maintaining its quality and safety of food products thus substituting synthetic plastic packaging materials.

Ultrasonicated resveratrol loaded starch nanocapsules: Characterization, bioactivity and release behaviour under in-vitro digestion

In this study, the resveratrol was nano-encapsulated in three different sources of starch like Water chestnut Horse chestnut and Lotus stem to safeguard it from gastric conditions and to improve its bioavailability and bioactivity upon digestion. The nano-capsules were prepared using safe and eco-friendly ultra-sonication method and studied for encapsulation-efficiency, particle-size and zeta-potential measurement. These were also characterized by ATR-FTIR, SEM, XRD and DSC. The release behaviour of resveratrol and its activity against anti-diabetic and anti-obesity were also studied. The particle size of HSR, LSR and WSR was found to be 419, 797 and 691 nm with a zeta potential of -16.09, -24.28 and -14.77 and encapsulation efficiency of 81.46, 75.83 and 79.37 %, respectively. The nanoparticles showed porous or film-like structures with decreased crystallinity and higher transition temperatures. The maximum percentage of resveratrol was released in intestinal juice and exhibited higher anti-obesity and anti-diabetic activities than free resveratrol after digestion.

Polysaccharide source altered ecological network, functional profile, and short-chain fatty acid production in a porcine gut microbiota.

Several validated dynamic in vitro models of the colon have been developed for humans, but there is no dynamic in vitro fermentation model for pigs. This study was conducted to modify the human, dynamic, computer-controlled TNO in vitro model of the colon (TIM-2) for pigs and investigate effects of different starch sources and polysaccharides on swine microbiota structure, ecological network, predictive functional profile, and short-chain fatty acids production. Our study showed that three different types of starch or two polysaccharides greatly impacted microbiota composition. Co-occurrence network analysis indicated that microbiota fed with different sources of starch changed the network topological properties. Functional profiles were predicted to vary significantly among the three starch treatments, and the original pig faecal inoculum was more similar to maize starch treatment. On the other hand, compared with maize starch and arabinoxylans (AX), the microbial composition of the original inoculum was more similar when AX-XG (arabinoxylans and xyloglucan) were added, and the functional profile of the original inoculum also clustered with AX-XG. The cumulative production of acetic, propionic, and butyric acid on maize starch were significantly higher than those on potato starch and wheat starch, while only the amount of acetic acid was significant higher on AX-XG than that on AX. In conclusion, supplementation of maize starch as the starch source together with AX and XG, leads to the bacteria being more stable in the in vitro model and closer to the original inoculum and microbial function compared to potato starch, wheat starch and AX. A maize basal diet may improve energy absorption in the large intestine in growing pigs.

Effect of different starch sources in a raw meat-based diet on fecal microbiome in dogs housed in a shelter.

A dietary intervention study was assessed to determine if different sources of starch in homemade diets could significantly modify fecal microbiome of dogs. Twenty-seven adult dogs were enrolled and fed a diet based on a mixture of rice and pasta with fresh raw meat (CD). After 90 d, 8 dogs continued to receive CD diet, 10 dogs received a diet made of a raw meat and a complementary food with rice as the main source of starch (B1), and 9 dogs were fed a diet with the same raw meat and a complementary food with potato as the main source of starch (B2). Samples of feces were collected from each dog in the mornings at the beginning of the study and after 15 d and analyzed for pH, ammonia N (N–NH3) and total N, short chain fatty acids (SCFA) and lactic acid. Relative abundance of fecal microbiota was assessed by sequencing and annotating the V3–V4 regions of the 16S rRNA. Total starch intake was similar between diets but differed in the in vitro rate digestion and in the resistant starch, which was higher in B2 than in B1 and CD diets. Dogs fed B2 diet showed lower (P < 0.05) N–NH3 and pH but higher (P < 0.05) molar proportion of lactic acid. Linear discriminant analysis of the genera relative abundances indicated a significant (P < 0.01) increase of SMB53 genus at the end of the study in B1 diet and of Megamonas genus in B1 and B2 diets in comparison to CD diet. These results suggest that changes of starch source in a raw meat-based diet have limited effects on fecal microbiome in healthy dogs, but underline a high variability of microbiota among dogs.

Properties of Carboxymethylated-Cassava and Sago Starches Prepared by using Sodium Monochloroacetate

In the present study, carboxymethyl starch (CMC) were produced from different sources of starch and their physico-chemical properties were evaluated. Carboxymethylation was performed using different concentrations of sodium monochloroacetate (1.1, 1.3 and 1.5 mol/mol of anhydrous glucose units) in a three-necked round-bottom flask (250 mL) for approximately 3 h (250 rpm and 40°C) in a two-stage reaction comprising alkalization and etherification. The introduction of carboxymethyl groups was confirmed in the results by the appearance of a new peak in the FTIR spectrum in 1650.10-1649.76 cm-1 region. In addition, degree of substitution (DS) of produced CMS was ranged from 0.53-0.60. An increase in the concentration of sodium monochloroacetate (1.1, 1.3 and 1.5 mol/mol of anhydrous glucose unit) resulted in greater paste clarity, higher solubility and greater swelling than native starch. Furthermore, cassava starch (tapioca) produced a CMS exhibited greater swelling than sago starch, however by contrast, CMS-sago was more soluble and clearer than CMS-cassava.

Fortification of Health Food Supplement as Functional Food in Sago Analogue

Health food supplement is a health support food that can help meet important nutrients for the body’s needs to function properly. Therefore, supplements are not full substitutes for medicines and food, meaning that health food supplements are intended to supplement or increase food intakes. This research is designed to create an economic multiplier effect through the development of sago analog rice as Riau’s flagship product. The purpose of this study is to process fortified sago analog rice fortified with health food supplements made from functional food ingredients (gabus fish concentrate, catfish oil and chlorella sp. concentrate). The research method used is an experimental method with experiments on processing sago analog rice fortified with health food supplements made from functional components of food (amino acid albuminl, crude fiber and omega-9 fatty acids). The treatments given are formulations of analog rice with four levels, namely: sago analog rice (F1), corn analog rice (F2), red rice analog rice (F3), and analog combination sago rice, red rice and corn (F4). The results show that, based on organoleptic preference tests, F4 analog rice was most preferred by panelists, namely analog rice combined with different sources of starch (sago, red rice and corn).

Combi-metal organic framework (Combi-MOF) of α-amylase and glucoamylase for one pot starch hydrolysis

The multi-enzyme biocatalyst allows to run in vitro multi-step cascade reactions in single pot. An efficient combi-metal organic frameworks (combi-MOF) of α-amylase and glucoamylase for one pot starch hydrolysis was constructed by mixing zinc acetate and 2‑methylimmidazole with enzyme mixture in one pot under biocompatible conditions. The prepared combi-MOF was characterized and analyzed by powder X-ray diffraction (PXRD), Fourier transform infrared (FT-IR), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM). Thermo-stability was evaluated for combi-MOF in the range of 55 to 75°C which showed three folds improved stability in terms of half-life. In kinetic parameter studies, rate of starch hydrolysis (Vmax) of combi-MOF was found to be enhanced after co-immobilization. Further, combi-MOF was recycled in batch mode which retained up to 52% residual activity after five successive cycles of reuse. In addition to that, combi-MOF exhibited extraordinary storage stability till 24days. At the end, starch hydrolytic activity of combi-MOF was tested for different sources of starch (corn, rice, wheat and potato) which exhibited higher rate of hydrolysis than mixture of free enzymes due to spatially co-localized multi-enzymatic systems.

Effects of different starch source of starter on small intestinal growth and endogenous GLP-2 secretion in preweaned lambs.

The objective of this study was to investigate the effects of different sources of starch in starter feed on small intestinal growth and endogenous glucagon-like peptide 2 (GLP-2) secretion in preweaned lambs. Twenty-four 10-d-old lambs were divided into three groups that were treated with different iso-starch diets containing purified cassava starch (CS, n = 8), maize starch (MS, n = 8), and pea starch (PS, n = 8). At 56 d old, there was no significant difference in final body weight (BW) of lambs among the three groups. However, different starch source in starter significantly affected the average daily feed intake (ADFI) and average daily gain (ADG) of lambs among three groups. Compared with the CS and MS diets, the PS diet significantly increased the GLP-2 concentration in blood plasma (P < 0.001), the crypt depth of the jejunum (P = 0.006), and the villus height of the ileum (P = 0.039). Meanwhile, PS diet significantly increased the mRNA expression of proglucagon and the glucagon-like peptide 2 receptor (GLP-2R) in the jejunum and ileum (P < 0.001). Furthermore, the PS diet significantly upregulated the mRNA expression of cyclin D1 (P < 0.001), cyclin E (P = 0.006), and cyclin-dependent kinases 6 (CDK6) (P = 0.048) in the jejunum and cyclin A (P < 0.001), cyclin D1 (P < 0.001), and CDK6 (P = 0.002) in the ileum. Correlation analysis showed that endogenous GLP-2 secretion was positively related to the mRNA levels of cell cycle proteins in small intestinal mucosa. In summary, all results showed that PS in starter feed promoted small intestinal growth that may, in part, be related to cell cycle acceleration and endogenous GLP-2 secretion in preweaned lambs. These findings provide new insights into nutritional interventions that promote the development of small intestines in young ruminants.

PRODUCTION OF AMYLASE FROM INDIGENOUSLY ISOLATED STRAIN OF Aureobasidium pullulans AND ITS HYPER PRODUCING MUTANT

New isolate obtained from leaf surfaces of Spinacia olevacea, Brassica oleracea and Coriandrum sativum was identified as Aureobasidium pullulans on the basis of cultural, morphological, biochemical and molecular characterization. The factors such as inoculums size, incubation time, sources of starch, and concentration of starch, sources of carbon and nitrogen, effect of initial pH and effect of temperature affecting production of amylase were optimized in present study. The optimum pH, temperature and incubation period for enzyme production were 5, 25OC and 5 days respectively. Among different sources of starch tested potato starch was shown to be the best. Potato starch at 1% was recorded to be the best concentration of starch for enzyme production. Sodium nitrate was ideal nitrogen source. At optimum conditions Aureobasidium pullulans Cau 19 has shown amylase activity 800U/L, which is twofold higher than before optimization. For the improvement of Aureobasidium pullulans Cau 19, the parental strain, after optimization of the cultural conditions, was subjected to UV irradiation for 8 min. Total 286 mutants were checked, out of which 9 mutant strains showing comparatively greater hydrolysis zone were selected for further study. Aureobasidium pullulans UVm 276 mutant has shown amylase activity which is 2.78 fold that of the wild type strain. Thus these findings have more impact on enzyme economy for biotechnological applications of microbial amylases.

Glucose and lipid profile of obese dogs fed with different starchy sources

ABSTRACT: Among the health disorders caused by obesity in dogs stand out hyperglycemia and hyperlipidemia. Glycemic responses are directly related to the amount and type of starch. Thus, this study aimed to evaluate the effect of different sources of starch on glycemic and lipid levels in obese dogs. These dogs were distributed in a completely randomized design in a factorial 3x2x2 (three sources starchy - corn (60%), sorghum (60%) and corn: sorghum (30:30%), two breeds - Beagle and Dachshund and males and females. After 28 days of experiment, dogs fed with corn had higher amounts of glucose (93.79A mg dL-1) (P&lt;0.05) compared with diets containing sorghum (88.00Bmg dL-1) and corn: sorghum (88.75B mg dL-1) (P&gt;0.05). Thus, dogs that consumed corn had a higher glycemic peak in a shorter time compared with dogs that ingested sorghum. Furthermore, the dogs fed with diet containing sorghum showed lower levels of fructosamine that dogs that ingested corn diet (P&lt;0.05) demonstrating that the diet containing sorghum maintained blood glucose over a longer time period that reduces the fluctuation of glucose in dogs. At 56 and 112 days of the experiment, a positive correlation between glucose levels and body weight (P&lt;0.05) was also observed. At 28, 56, 84 and 112 days, Beagle dogs showed higher serum concentrations of total cholesterol compared with Dachshund dogs (P&lt;0.05). It was concluded that the starchy source and breed can interfere with metabolic rates of obese dogs.

Gastrointestinal tract development in fattening lambs fed diets with different amylose to amylopectin ratios

Thirty-six, 7-d-old male lambs of similar weights were used in the current study to examine the effects of dietary amylose to amylopectin ratios (amylose/amylopectin) on gastrointestinal development in fattening lambs fed concentrates with different sources of starch (tapioca, wheat, maize, and pea) and amylose/amylopectin (0.12, 0.23, 0.24, and 0.48, respectively). The maize starch (MS) and wheat starch (WS) diets improved weight and volume of gastrointestinal. The pea starch diet significantly increased papillar height (P &lt; 0.001), papillar surface area (P = 0.019), and density of papillae (P = 0.001) in the rumen. Additionally, the pea starch diet significantly enhanced villus height, crypt depth, and villus surface area and villus/crypt ratio (P &lt; 0.05) in small intestine. Expressions of insulin-like growth factors I (IGF-1) and insulin-like growth factors’ receptors (IGF-1R) significantly increased in the duodenum mucosa (P = 0.021, P = 0.006, respectively), jejunum mucosa (P = 0.002, P = 0.005, respectively), and ileum mucosa (P = 0.003, P &lt; 0.01, respectively) in the pea starch diet group. The results of the present study show that MS and WS accelerated physical development of rumen and intestine compartments, whereas pea starch increased development of their some morphological parameters, possibly through enhanced expression of genes such as IGF-1 and IGF-1R in fattening lambs.

Effect of the storage conditions on mechanical properties and microstructure of biodegradable baked starch foams

ABSTRACTStarch-based foams (SBFs) were prepared with corn, potato, tapioca, and chayotextle starches. A compression baking process was used to develop the SBFs. Biodegradation studies showed that different sources of starch have a particular behavior. The starches showed values of degradation of ~85%, while the degradation of SBFs was ~70%. The SBFs were conditioned at relative humidities (RHs) of 0 and 75% and temperatures of 4 and 65°C. The starch source used to prepare the SBFs did not have an effect on the mechanical properties, but the storage conditions showed a significant effect on those properties; an increase of RH causes an increase in the mechanical properties. A similar behavior was observed in the conditioned SBFs at different temperatures. The increase of the storage temperature showed a slight reduction in the values of elongation at break. Microscopy revealed that the conditions of RH and storage temperature affect the internal structure of the SBFs.

INCLUSÃO DE DIFERENTES FONTES DE AMIDO NA DIETA DE JUNDIÁS (Rhamdia quelen): PARÂMETROS METABÓLICOS E BIOQUÍMICOS

This study was conducted to evaluate the influence of ingestion of different sources of starch on enzymatic and metabolic indicators in blood and liver of Rhamdia quelen. The experiment was conducted during 20 days in water recirculation system. Sixty fishes (initial average weight 60g) were used and divided into three treatments composed of the following diets: inclusion of peeled oats, defatted rice bran and control diet, all with 36% crude protein and 3,200 Kcal DE/Kg. There was no significant difference in growth parameters. The analysis demonstrated a reduction in liver glycogen content for the treatment with oats; however, no apparent changes in the activity of the enzymes alanine-aminotransferase and glucose-6-phosphatase were observed, raising the possibility that the fishes did not use gluconeogenesis. The blood analysis, showed no significant differences in the determinations of total cholesterol, HDL cholesterol and albumin; however, serum levels of total protein were higher for treatment with defatted rice bran, and triglyceride levels were lower in animals that received diet containing oat as starch source. It can be concluded that Rhamdia quelen is able to metabolize various sources of carbohydrate, without the need to use either diet or body protein as an energy source.KEYWORDS: carbohydrate; fish nutrition; intermediary metabolism.

Hindgut fermentation in pigs induced by diets with different sources of starch

A proportion of dietary starch reaches the hindgut, being fermented there. The characteristics of in vitro caecal fermentation and microbial community in pigs given different sources of starch were studied. Twenty-four Duroc × (Landrace × Large White) gilts given diets based on barley (B), broken rice (R), maize (M) or peas (P) (n=6) for five weeks were slaughtered with 93.6 ± 6.41 kg. No differences (p&gt;0.10) were recorded in caecal pH, total short chain fatty acid (SCFA) and total bacterial concentration, nor in in vitro gas production from caecal contents, indicating the lack of a quantitative dietary effect on caecal environment. This could be partly due to the length of fasting time before slaughter (around 10 h). Molar SCFA proportions did not differ among diets; however, relative proportion of Lactobacillus sobrius/amylovorus as the species-type in starch digestion in hindgut of pigs, was highest with P diet (p = 0.010), and gas production from potato starch as substrate with P diet was highest at 2 h incubation (p = 0.012), and higher than B and R diets at 4 (p = 0.055) and 6 (p = 0.10) h incubation. Caecal bacterial biodiversity was higher for M and R diets than for P and B diets (Shannon index, p = 0.003). Sources of resistant or slowly digestible starch such as peas promote a microbial community with a different profile and higher capacity to ferment the starch arriving to the organ than other sources which are mostly digested in the small gut.

The &lt;i&gt;in vitro&lt;/i&gt; caecal fermentation of different starch sources in rabbits

The objective of our study was to extend the knowledge of microbial fermentation of starch in the rabbit caecum using the in vitro gas production technique. Different sources of starch (wheat and maize grain, raw and cooked potato) and different starch isolates (wheat, maize and potato starch) were incubated with inoculum, prepared from rabbit caecum content. The gas production parameters such as total potential gas production (parameter “B”), gas production till 10 h of incubation (Gas10), maximum fermentation rate (MFR) and time of maximum fermentation rate (TMFR) were estimated with Gompertz model and were significantly affected by the substrata. Differences between maize grain and maize starch were small: MFR and TMFR were not different, while Gas10 of maize starch was higher than from maize grain (5 and 3 ml/kg DM, respectively; P &lt; 0.05). MFR of wheat starch was higher than that of wheat grain (3.1 and 2.4 ml/h, respectively; P &lt; 0.05), but reached maximum rate later (TMFR: 12.4 and 10.9 h, respectively; P &lt; 0.05) and had lower Gas10 (6 and 9 ml/kg DM, respectively; P &lt; 0.05). The fermentation of cooked potato reached the TMFR after 8.5 h of incubation and had the highest MFR (2.9 ml/h) and Gas10 (16 ml/kg DM) of all substrata. The MFRs of raw potato and potato starch were similar (2.1 and 2.3 ml/h, respectively), although TMFR and Gas10 of raw potato were shorter and higher than those of potato starch (TMFR of 10.7 and 17.2 h and Gas10 of 10 and 1 ml/kg DM, respectively; P &lt; 0.05).

Effect of replacing barley with corn or sorghum grain on rumen fermentation characteristics and performance of Iranian Baluchi lamb fed high concentrate rations

The present study, conducted in Iran, was aimed to evaluate the effects of partial substitution of barley with corn or sorghum (slowly fermenting grains) on in situ dry matter (DM) and starch degradability and in vivo digestion characteristics and performance in finishing Iranian Baluchi lambs. In the first experiment, samples of ground barley (B), corn (C), sorghum (S) and their combinations [%: 70 barley : 30 corn (LC), 70 barley : 30 sorghum (LS), 30 barley : 70 corn (HC) and 30 barley : 70 sorghum (HS)] were incubated in nylon bags in rumen of three fistulated Holstein steers. Effective degradability (ED) for DM and starch were different (P &lt; 0.05) among treatments. In both cases, ED was higher (P &lt; 0.05) for B than that for C, S, HC and HS. Results of the present study confirmed previous reports that ruminal degradability of barley is higher than that of corn and sorghum, and further suggested that there may be benefits in replacing barley with ~70% of corn or sorghum. In Experiment 2, 30 Iranian Baluchi male lambs weaned at 65 days of age (BW 32 ± 2.3 kg) were divided into five equal weight groups (6 animals per group) in a complete randomised design. Lambs received a mixed diet in the form of total mixed rations (TMR), consisting of 32.5% alfalfa hay [89% DM, 43% natural detergent fibre (NDF) and 16% crude protein (CP) in diet DM basis] and 67.5% of the respective concentrates. The five iso-caloric (10.73 MJ ME/kg) and iso-nitrogenous (14% CP/kg DM) concentrates with different sources of starch used in the study were (1) barley, as the control cereal source for starch, and in the other treatments barley substituted with (2) 30% corn (LC), (3) 30% sorghum (LS), (4) 70% corn (HC) and (5) 70% sorghum (HS). Lambs in HC and HS diets recorded increases of 20 and 50 g/day in average daily gain, respectively, compared with lambs in B. Substitution of B with 70% C and S significantly (P &lt; 0.01) improved ruminal pH and, presumably, resulted in higher starch outflow for post-ruminal digestion, as reflected by a higher blood glucose concentration, particularly for lambs in the HS diet. Results of the present study reaffirmed the advantages of feeding a mixture of grains with differing fermentation rates and, as previously reported, also suggested that the inclusion rate of slowly degradable grains should be ~70% so as to enhance animal performance.

Growth performance and nitrogen metabolism in weaned pigs fed diets containing different sources of starch

Abstract This study was conducted to determine the performance and N metabolism in weaned pigs fed diets containing different sources of starch. Pigs were weaned at 28 days of age, and assigned randomly into 4 soybean meal-based diets containing different sources of starch: corn, brown rice, sticky rice, and Hi-Maize 1043 (resistant starch). There were 12 pigs per treatment group in the growth-performance trial. Additionally, a 4 × 4 Latin design was used for a nitrogen (N) balance study, with 5 days for each experimental period ( n = 4). Average daily gains were 0.38, 0.34, 0.31, and 0.28 kg/day ( P P P

Consumption of resistant starch decreases lipogenesis in adipose tissues but not in muscular tissues of growing pigs

The aim of the present study was to evaluate the effect of two different sources of starch on plasma glucose, acetate and insulin responses and peripheral lipogenesis in adipose and skeletal muscle tissues. Eighteen male growing pigs were fed a diet containing 250 g native corn starch/kg (CS; 26% amylose, 74% amylopectin) or 250 g raw potato starch/kg (RPS; 20% amylose, 80% amylopectin) as examples of digestible and resistant starch (type II), respectively. After 38 days on the experimental diets, twelve pigs (6 per treatment, Experiment 1) were killed and samples of adipose and muscular tissues were analysed for intramuscular lipid content and lipogenic enzyme activity. Lipogenic enzyme activities were significantly higher for CS than RPS in adipose tissues but not in muscular tissues. No differences were detected on the lipid content of the muscles tested. The six remaining pigs (3 per treatment, Experiment 2) were fitted with catheters in the saphenous vein and femoral artery after 28 days on the experimental diets. After feeding restoration to a level of 1.1 kg/day, they received a primed constant infusion of 1- 13C acetate for a period of 90 min, starting 5 h after feeding the meal ingestion, and a primed constant infusion of 6,6-D 2 glucose for 7 h, starting 1 h before the following meal ingestion. No differences were observed between diets on peripheral acetate entry rate. Glucose concentration, the rate of peripheral glucose appearance and insulin concentration were quantitatively higher after the meal for CS than for RPS diet. This shows the importance of type of dietary starch on lipogenesis as a result of changes in glycemia and insulinemia in adipose but not in muscular tissues of growing pigs.