Different Locations In China Research Articles

Morphology and molecular phylogeny of Pelagothrix abietum (Penard, 1922) comb. nov. and two new Metacystis and Apolagynus species (Ciliophora, Prostomatea)

Ciliates of the class Prostomatea show a broad spectrum of feeding strategies and often occur abundantly in various aquatic habitats, playing a vital role in the biogeochemical cycle. Due to their small cell size and simple structure, prostomateans were considered to be a group with a lower degree of morphological differentiation for a long time. However, recent research suggests that the diversity of this group of ciliates is higher than previously thought. In the present study, three prostomateans, collected from different localities in China and classified into three families, were examined using morphological and phylogenetic techniques. Our analyses revealed two new species, Metacystis multitricha sp. nov. and Apolagynus spiralis sp. nov., and suggested a transfer of Prorodon abietum Penard, 1922 to the genus Pelagothrix. Phylogenetic analyses corroborated the morphological classifications of the three species into the families Metacystidae, Lagynusidae, and Holophryidae, respectively, demonstrating that their diagnostic characteristics bear an evolutionary signal at the family level.

The first report and biological characterization of Avian Orthoavulavirus 16 in wild migratory waterfowl and domestic poultry in China reveal a potential threat to birds

ABSTRACT The Avulavirus within the family Paramyxoviridae includes at least 22 different species, and is known to cause different types of infections and even be fatal in multiple avian species. There is limited knowledge of the genetic and biological information of Avulavirus species 2 to 22 in domestic and wild birds and the disease significance of these viruses in birds is not fully determined, although as many as 10 new distinct species have been identified from wild birds and domestic poultry around the world in the last decade. This study aimed to use PCR, virus isolation, and sequencing to genetically and biologically characterize Avian Orthoavulavirus 16 (AOAV-16) in wild birds and domestic poultry collected from different locations in China between 2014 and 2022. Of five isolated AOAV-16 strains (Y1 to Y5), only the Y4 strain had a haemagglutination (HA)-negative result. All of these isolates were low virulent viruses for chickens, except Y3 which was detected simultaneously with avian influenza virus (AIV) of H9N2 subtype. Furthermore, at least four different types of intergenic sequences (IGS) between the HN and L genes junction, and recombination events, as well as interspecific transmission by wild migratory birds, existed within the species AOAV-16. These findings and results of other reported AOAV-16 strains recommend strict control measures to limit contact between wild migratory birds and domestic poultry and imply potential threats to commercial poultry and even public health challenges worldwide. RESEARCH HIGHLIGHTS First confirmation of AOAV-16 in domestic and wild birds in China. AOAV-16 are low virulent viruses for chickens. Co-circulation/co-infection of AOAV-16 and H9N2 subtype AIV enhanced pathogenicity. Different intergenic sequences and recombination events exist within AOAV-16.

“To do or not to do”, male nurses’ experiences of providing intimate care to female patients in China, a constructivist grounded theory study

BackgroundSome studies suggest that female patients have more concerns about receiving intimate care from male than female nurses. Thus, providing intimate care to female patients is a challenging experience for male nurses. The purpose of this study was to explore Chinese male nurses’ experiences and process of providing intimate clinical care to female patients.MethodsA constructivist grounded theory approach was used to develop a theoretical understanding of male nurses’ experiences. This study included participants from 3 hospitals in different locations in China. Twenty-five male nurses were recruited using purposive and theoretical sampling. Semi-structured interviews were conducted. Data analysis was completed using initial coding, focused coding, theoretical coding and memo writing to produce core concepts and categories, and theory development.ResultsChinese male nurses’ experiences of providing intimate care to female patients can be constructed as a three-stage process: (1) anticipation of the level of embarrassment, (2) deciding on the process: do it or not do it and (3) protecting both parties and dealing with embarrassment. Additionally, seven themes and associated categories were identified to represent the important factors in the process of male nurses providing intimate care to female patients in China.ConclusionsChinese traditional culture may affect the embarrassment in Chinese male nurses providing intimate care to female patients. The embarrassing situation can be divided into three different stages, and male nurses have different main concerns in each stage. Hospital nursing administrators should consider the experiences and needs of male nurses in providing intimate care and provide them with psychological support, education and training.

Structure and functional characteristics of starch from different hulled oats cultivated in China

This work aimed to evaluate the structure and functional characteristics of starch from ten hulled oat cultivars grown in different locations in China. The protein, phosphorus, amylose, and starch contents were 0.2–0.4 %, 475.7–691.8 ppm, 16.2–23.0 %, and 93.6–96.7 %, respectively. All the starches showed irregular polygonal shapes and A-type crystallization with molecular weights ranging from 7.2 × 107 to 4.5 × 108 g/mol. The amounts of amylopectin A (DP 6–12), B1 (DP 13–24), B2 (DP 25–36), and B3 (DP > 36) chains were in the ranges of 10.3–16.0 %, 54.5–64.8 %, 16.5–21.1 %, and 4.9–13.1 %, respectively. The starches differed significantly in gelatinization temperatures, pasting viscosity, solubility, swelling power, rheological properties, and digestion parameters. The results revealed that the larger particle size could increase the peak viscosity of the starch paste. The presence of phosphorus increased the gelatinization temperature and enhanced the resistant starch content. The starch granules with higher crystallinity contained a higher proportion of phosphate, which increased final viscosity and setback viscosity but decreased rapidly digestible starch. Overall, oat starch with a high phosphorus content could be used to prepare low-glycemic-index food for diabetes patients.

Evaluating the Technical, Economic, and Environmental Performance of Solar Water Heating System for Residential Applications–Comparison of Two Different Working Fluids (Water and Glycol)

The use of solar water heaters (SWH) in both residential and commercial facilities is one of the possible ways to reduce electricity bills and the release of greenhouse gases (GHG). This study assessed the technical, economic, and environmental performance of a SWH system at six different locations in China (i.e., Lhasa, Lauchang, Wuhan, Kashi, Yumen, and Harbin). A comparison between two different working fluids (i.e., water and glycol) were modeled in the System Advisor Model in all six cities. A sensitivity analysis was conducted on some key technical and economic parameters to assess the impact of such parameters on the performance of SWH systems in the country. According to the results, Lhasa recorded the highest capacity factor of 11% and 10.70% using water and glycol as the working fluid, respectively. Lhasa was identified as the best location among the studied locations due to its high solar irradiation. The optimization study indicates that the optimum azimuth for China is 190°. It was also found that a 25% reduction in the outlet set temperature of the water can reduce the capacity factor from 11% to about 9.2%. Using the SWH as simulated in this study can reduce carbon dioxide emissions from 1252.87–2014.85 kg per year to 138.20–330.23 kg per year; the extent of reduction depends on the location of the SWHS, and the solar energy available at the area. Net electricity bill savings of $156–296 could be obtained if SWH systems were installed and used at the studied locations.

Residue determination and dietary risk assessment of mesotrione, nicosulfuron, atrazine and its four metabolites in maize in China

IntroductionTo improve maize yield in China, multiple herbicides have been simultaneously applied to control more weeds. However, this combined application raises concerns about potential residues and their subsequent risks to human health. Therefore, evaluating the residues and dietary risk of new herbicide formulations is critical for the sustainability of maize production.MethodsUsing UHPLC-MS/MS, we developed quick methods for the determination of residues of mesotrione, nicosulfuron, atrazine and its four metabolites with acceptable accuracy and precision. The limits of quantification (LOQs) were 0.01 mg/kg for mesotrione and atrazine-desethyl-desopropyl, and 0.005 mg/kg for nicosulfuron, atrazine, 6-deisopropyl atrazine, 2 hydroxyatrazine, and deethylatrazine. Field trials were conducted at 12 different locations in China. And the risk quotient (RQ) model was used to evaluate the chronic risk of residues of these herbicides.ResultsThe residues of straw samples were in the ranges of <0.01 mg/kg for mesotrione, <0.005–0.010 mg/kg for nicosulfuron, and <0.037–0.254 mg/kg for accumulated atrazine. In green straw, the observed ranges were <0.01 mg/kg for mesotrione, <0.005–0.007 mg/kg for nicosulfuron, and <0.037–0.211 mg/kg for accumulated atrazine. Meanwhile, for both green and fully-grown maize, residues were <0.01 mg/kg for mesotrione, <0.005 mg/kg for nicosulfuron, and <0.037 mg/kg for accumulated atrazine. And all RQ values of green and fully-grown maize were below 100%.ConclusionBased on the RQ model, the dietary risk of exposure to three herbicides through maize was acceptable by consumers. This study helps guide the rational use of mesotrione, nicosulfuron and atrazine to ensure the safe production of maize and our human health.

Discrimination of Dioscorea species (Chinese yam) using FT-IR integrated with chemometric approach

Dioscorea oppositifolia is an important crop and functional food. D. oppositifolia tuber is often adulterated with D. persimilis, D. alata, and D. fordii tuber in the commercial market. This study proposed an integrated Fourier transform infrared spectroscopy (FT-IR) with chemometric approach to differentiate these four Dioscorea species. A total of 107 Dioscorea spp. tuber samples were collected from different locations in China. Principal Component Analysis (PCA), PCA-Class, and Orthogonal Partial Least Square Discriminant Analysis (OPLS-DA) were utilised to classify the FT-IR spectra. In this PCA is unable to differentiate the Dioscorea spp. tuber effectively. However, PCA-Class and OPLS-DA can distinguish spp. these 4 species Dioscorea tuber with high accuracy, sensitivity, and specificity. Additionally, the RMSEE, RMSEP and RMSECV values for OPLS-DA model were low, showing that it is a good model. The combination of FT-IR with the PCA-Class and OPLS-DA is practical in discriminating Dioscorea spp. tubers.

New data on the subfamily Charipinae (Hymenoptera: Figitidae) of China with the first description of Allloxysta xanthopa (Thomson, 1862) male and an updated key

ABSTRACT Material collected from different locations of China as part of a National Natural Science Foundation of China project has been revised. A total of 16 specimens of Charipinae have been examined, grouped in eight species: Alloxysta arcuata (Kieffer, 1902), A. castanea (Hartig, 1841), A. pilipennis (Hartig, 1840), A. pilosa Ferrer-Suay & Pujade-Villar, 2013, A. sawoniewiczi (Kierych, 1988), A. xanthopa (Thomson, 1862), Phaenoglyphis longicornis (Hartig, 1840) and P. villosa (Hartig, 1841). Phaenoglyphis longicornis (Hartig, 1840) is a new record from China and male of Alloxysta xanthopa (Thomson, 1862) is here described and illustrated for the first time. Information about this material is provided and an updated key to genera and species of Charipinae from China is presented.

Techno-economic evaluation of photovoltaic thermal system integrated with porous phase change materials: Case studies in China

This study aims to to develope a comprehensive mathematical model for predicting the performance, environmental impact, and economic viability of solar photovoltaic thermal (PVT) systems, with specific focus on the use of Porous Phase Change Material (PPCM) into a PVT system (PVT-PPCM) and its effect on the cooling cycle temperature throughout the year. The model was experimentally validated and implemented in Matlab to assess the performance of various systems, including PV-only, PVT, and PV integrated with PCM (PV-PCM). To determine the optimum melting point temperature for the PCM-based systems, a sensitivity analysis was conducted for different locations in China. The annual performance, CO2 reduction, discounted payback time (DPBT), and Levelized cost of energy (LCOE) were predicted for each system. Subsidy-free method was used for economic evaluation, which only shows system’s cost savings. In addition, it is worth noting that the maximum power output of each system was evaluated to be 1 MW. The results of the study showed that PVT-PPCM exhibited the highest annual power output of 1920 MWh and CO2 reduction of 30 tons/year, while the LCOE ranged from 0.02 to 0.58 CNY/kWh and the DPBT varied from 5 to 20 years across the case studies. The study also compared the Levelized cost of electricity (LCOEel) of PVT and PVT-PPCM with cases in UK and Spain, with the lowest LCOEel of PVT recorded in Kunming at 0.4 CNY/kWh.The significance of this study lies in its identification of the strengths and weaknesses of PVT system designs across different climates, highlighting areas for improvement. By providing insights into system performance, environmental impact, and economic feasibility, this research contributes to the advancement and optimization of PVT technology for sustainable energy applications.

Proposal of Four New Aureobasidium Species for Exopolysaccharide Production.

In this study, 99 strains of Aureobasidium species were isolated from various samples collected from different locations in China, among which 14 isolates showed different morphological characteristics to other strains identified as known Aureobasidium species. Based on morphological characteristics, those 14 strains were classified into four groups, represented by stains of KCL139, MDSC-10, XZY411-4, and MQL9-100, respectively. Molecular analysis of the internal transcriptional spacer (ITS) and part of the large ribosome subunit (D1/D2 domains) indicated that those four groups represent four new species in the Aureobasidium. Therefore, the names Aureobasidium insectorum sp. nov., A. planticola sp. nov., A. motuoense sp. nov., and A. intercalariosporum sp. nov. are proposed for KCL139, MDSC-10, XZY411-4, and MQL9-100, respectively. We also found that there were differences in the yield of exopolysaccharides (EPS) among and within species, indicating strain-related exopolysaccharide-producing diversity.

Analysis of the rule of window-to-wall ratio on energy demand of residential buildings in different locations in China

Building energy demands are influenced by the window-to-wall ratio (WWR). The impact of the WWR on the energy demand in the same area has been researched by software. However, the impact of the WWR on the energy demand of buildings in different locations of China has not been investigated using the energy balance equation. The equation for the indoor temperature variation under various internal and external disturbances was provided in this study. The relationship between parameters A and B and the change in indoor temperature was proven. China's Harbin, Beijing, and Chengdu air-conditioning and heating loads, as well as the impact of changing WWR on the rate of energy savings for air-conditioning and heating energy demand, were studied. After investigating the changes in the cooling and heating loads in various cities under various WWRs, the suitable relationship between the cooling and heating loads and the WWR of three urban units was discovered. The findings indicated that despite the three cities' various climates and the diverse energy demands for air conditioning and heating in each city, with the same change in the WWR of urban buildings, the change rate of the air conditioning and heating loads in each city was near. Meanwhile, the heating and cooling loads were linear with WWR.

Morphological and Molecular Characterizations of Three Species of the Genus Synura (Synurales, Chrysophyceae) from China

Three species of the genus Synura from China are described here. Morphological observations and molecular phylogenetic analyses were conducted for three specimens collected at different locations in China. The described morphological features included cell size, scale size, spines, keels, and struts. Molecular analyses based on multiple genetic markers (SSU and LSU rDNA and internal transcribed spacer rDNA) were used to determine the phylogenetic positions of the three Synura species. Morphologically, specimen GZ201017 collected in Guizhou Province was characterized by a well-developed keel and lanceolate scales; specimen SX210304 collected in Shanxi Province was characterized by a less-developed keel and poor silicification; and specimen GD201126 collected in Guangdong Province was characterized mainly by spines with blunt ends or two small teeth on the tips. The morphotypes GZ201017, SX210304, and GD201126 corresponded to the original descriptions of Synura petersenii, S. glabra, and S. longitubularis, respectively. This discovery laid a foundation for the molecular phylogeny of the genus Synura and an enhanced understanding of Synura diversity and distribution in China.

Differences in the Chloroplast Genome and Its Regulatory Network among Cathaya argyrophylla Populations from Different Locations in China.

Cathaya argyrophylla Chun et Kuang is a severely endangered, tertiary relict plant unique to China whose high physiological sensitivity to the environment, including photosensitivity, is likely closely related to its endangered status; however, the exact mechanism responsible has remained unknown due to the rarity of the plant and the difficulties involved in performing physiological studies on the molecular level. In this study, the chloroplast genomes of six C. argyrophylla populations sampled from different locations in China were characterized and compared. In addition, a gene regulatory network of the polymorphic chloroplast genes responsible for regulating genes found elsewhere in the plant genome was constructed. The result of the genome characterization and comparison showed that the genome characteristics, the gene composition, and the gene sequence of the chloroplast genes varied by location, and the gene regulatory network showed that the differences in growth location may have led to variations in the protein-coding chloroplast gene via various metabolic processes. These findings provide new insights into the relationship between chloroplasts and the sensitive metabolism of C. argyrophylla and provide additional reference materials for the conservation of this endangered plant.

The Threat of Potentially Pathogenic Bacteria in the Feces of Bats.

Bats have attracted global attention because of their zoonotic association with severe acute respiratory syndrome coronavirus (SARS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Previous and ongoing studies have predominantly focused on bat-borne viruses; however, the prevalence or abundance of bat-borne pathogenic bacteria and their potential public health significance have largely been neglected. For the first time, this study used both metataxonomics (16S rRNA marker gene sequencing) and culturomics (traditional culture methods) to systematically evaluate the potential public health significance of bat fecal pathogenic bacteria. To this end, fecal samples were obtained from five bat species across different locations in China, and their microbiota composition was analyzed. The results revealed that the bat microbiome was most commonly dominated by Proteobacteria, while the strictly anaerobic phylum Bacteroidetes occupied 35.3% of the relative abundance in Rousettus spp. and 36.3% in Hipposideros spp., but less than 2.7% in the other three bat species (Taphozous spp., Rhinolophus spp., and Myotis spp.). We detected 480 species-level phylotypes (SLPs) with PacBio sequencing, including 89 known species, 330 potentially new species, and 61 potentially higher taxa. In addition, a total of 325 species were identified by culturomics, and these were classified into 242 named species and 83 potentially novel species. Of note, 32 of the 89 (36.0%) known species revealed by PacBio sequencing were found to be pathogenic bacteria, and 69 of the 242 (28.5%) known species isolated by culturomics were harmful to people, animals, or plants. Additionally, nearly 40 potential novel species which may be potential bacterial pathogens were identified. IMPORTANCE Bats are one of the most diverse and widely distributed groups of mammals living in close proximity to humans. In recent years, bat-borne viruses and the viral zoonotic diseases associated with bats have been studied in great detail. However, the prevalence and abundance of pathogenic bacteria in bats have been largely ignored. This study used high-throughput sequencing techniques (metataxonomics) in combination with traditional culture methods (culturomics) to analyze the bacterial flora in bat feces from different species of bats in China, revealing that bats are natural hosts of pathogenic bacteria and carry many unknown bacteria. The results of this study can be used as guidance for future investigations of bacterial pathogens in bats.

DIVERSITY, MUTATION AND RECOMBINATION ANALYSIS OF CHILLI INFECTING POTYVIRUSES FROM SOUTHEAST ASIA AND CHINA

The chilli ring spot virus (ChiRSV) is one of the most important virus of chilli crop in Southeast Asia and China. Chilli ring spot virus (ChiRSV) is most devastating virus not only for chilli crop but also for solanaceous crops. In severe cases, up to 100% yield losses have been observed in chilli crop. A large virus vector population spreads this disease throughout Southeast Asia and China. The diversity of this Potyvirus is proven after examination of many samples isolated from different locations in China and Vietnam, which shows the virus is successfully prevalent in that part of the world. The phylogenetic investigation of this potyvirus included 11 full size samples in total. Phylogenetic study of different samples indicates that two sequences (KT633930, KX379001) are ancestors of all sequences and others are descendants. Later on, with the passage of time, these sequences (KT633930, KX379001) evolve and different strains, variants and species arrived. Recombination analysis shows that there is no recombination in any sequence and all sequences are quite unique. We also determined replacement frequencies for Potyviruses. According to our findings, ChiRSV is very important pathogen for not only Solanaceae family but also for other crops because of its presence on different other host plants.

Efficient Catalyst-Free Henry Reaction between Nitroalkanes and Aldehydes or Trifluoromethyl Ketones Promoted by Tap Water

AbstractThe first examples of highly effective Henry reactions between nitroalkanes and aldehydes or trifluoromethyl ketones that proceed under catalyst-free and additive-free conditions, in a recyclable tap water medium, and at room temperature are reported. This process tolerates a broad range of aldehydes and trifluoromethyl ketones to give a series of β-nitro alcohol products in excellent yields. Such products are widely used in the syntheses of pharmaceutical intermediates and natural products. This protocol can be successfully scaled up to a 50-mmol scale without a reduction in yield. Tap water from different locations in China exhibited pH values ranging from 7.5 to 8.1, but the varying pH had no effect on the yield and the processes were successfully reproduced. Finally, the tap water was effectively recovered and reused without any postprocessing, even when the reaction substrates were different.

Geographically Associated Fungus-Bacterium Interactions Contribute to the Formation of Geography-Dependent Flavor during High-Complexity Spontaneous Fermentation.

ABSTRACTFermented foods often have attractive flavor characteristics to meet various human demands. An ever-challenging target is the production of fermented foods with equal flavor profiles outside the product’s origin. However, the formation of geography-dependent flavor in high-complexity fermentations remains poorly understood. Here, taking Chinese liquor (baijiu) fermentation as an example, we collected 403 samples from 9 different locations in China across a latitude range of 27°N to 37°N. We revealed and validated the geography-dependent flavor formation patterns by using culture-independent (metabolomics, metagenomics, and metatranscriptomics) and culture-dependent tools. We found that the baijiu microbiomes along with their metabolites were flavor related and geography dependent. The geographical characteristics were determined mainly by 20 to 40 differentiated chemical markers in metabolites and the latitude-dependent fungal structure of the microbiome. About 48 to 156 core microbiota members out of 735 bacterial genera and 290 fungal genera contributed to the chemical markers. The contributions of both fungi and bacteria were greater than those from either bacteria or fungi alone. Representatively, we revealed that dynamic interdependent interactions between yeasts and Lactobacillus facilitated the metabolism of heterocyclic flavor chemicals such as 2-acetylpyrrole, 2,3,5-trimethylpyrazine, and 2-acetylfuran. Moreover, we found that the intraspecific genomic diversity and microbial structure were two biotic factors that contributed to dynamic microbiome assembly. Based on the assembly pattern, adjusting the composition and distribution of initial species was one option to regulate the formation of diverse flavor characteristics. Our study provided a rationale for developing a microbiome design to achieve a defined flavor goal.IMPORTANCE People consume many spontaneously fermented foods and beverages with different flavors on a daily basis. One crucial and hotly discussed question is how to reproduce fermented food flavor without geographical limitations to meet diverse human demands. The constantly enriched knowledge of the microbial contribution to fermented flavor offers valuable insights into flavor biotechnological development. However, we still have a poor understanding of what factors limit the reproduction of fermented flavor outside the product’s origin in high-complexity spontaneous fermentations. Here, taking baijiu fermentation as an example, we revealed that geography-dependent flavor was contributed mainly by fungus-bacterium cooperative metabolism. The distinct initial microbial composition, distribution, and intraspecific genomic diversity limited reproducible microbial interactions and metabolism in different geographical areas. The abundant microbial resources and predicted fungus-bacterium interactions found in baijiu fermentation enable us to design a synthetic microbial community to reproduce desired flavor profiles in the future.

Changes in Triacylglycerols Content and Quality Control Implications of Coix Seeds during Processing and Storage.

Coix seed is a kind of widespread cereal, and it is used as a folk medicine in China. The present work focuses on the analysis of changes in triacylglycerols (TAGs) content and mycotoxins of coix seed during the processing and storage period for its quality control. Using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS) and high-performance liquid chromatography coupled with evaporative light-scattering detector (HPLC-ELSD) techniques, 42 lipid components in coix seeds were identified, and seven molecular species of TAG in coix seeds from different localities in China were measured and compared, respectively. A correlation analysis between the morphological features and TAGs contents revealed the integrity instead of the particle size of the seed, displaying a highly positive correlation with its quality. The higher contents of TAGs in hulled coix seed than in polished coix seed proposed an alternative processing way. During storage, the changes in TAGs contents of seeds indicated that the storage period should be less than 3 months, and the intact seeds could maintain lipid stability better than the powder. Furthermore, the air humidity and temperature should be controlled during coix seed storage to prevent the production of mycotoxins. These results provide significant insight into the effective control of coix seed quality during processing and storage.

Concentration and risk assessment of PCNs in green tea in different locations in China

Polychlorinated naphthalenes (PCNs) were added to the Stockholm Convention list of persistent organic pollutants in 2015. PCNs are mainly unintentionally produced during industrial processes nowadays, and can be widely found in environmental media and foodstuffs. Dietary intake is the primary pathway for human exposure to PCNs. PCNs in different categories of foodstuffs have been reported. However, little information on PCN concentrations in green tea, a popular beverage worldwide is available. In this study, all 75 PCN congener concentrations and distributions in green tea samples (n = 102) from 11 regions in China were determined, and risk assessment of human exposure to PCNs through tea consumption was conducted. The PCN concentrations in all the green tea samples were 3.62–175 pg/g dry weight (mean 36.1 pg/g dry weight). Similar PCN homolog and congener profiles were found in green tea samples from different areas. The dominant PCN homologs in all of the green tea samples were di-CNs, tetra-CNs, and tri-CNs. No direct relationships were found between PCN emission sources and PCN concentrations in the green tea samples. The brewing technique could affect the PCN concentrations and homolog profiles in tea leaves. PCNs in green tea from China were found to pose little risk to humans.

Techno-economic evaluation of electric vehicle charging stations based on hybrid renewable energy in China

The purpose of the study is to investigate the technical and economic feasibility of hybrid solar photovoltaic (PV) and wind turbine (WT) power systems for environment-friendly electric vehicle (EV) charging stations at five different locations in China. The HOMER Pro 3.14 software using derivative-free algorithm has been used to search for the optimal scheme of the charging stations. The results show that the best solution considering renewable energy charging stations in the five regions is the hybrid PV/WT/battery EV charging station. Furthermore, the PV/WT/battery charging station for Nanjing is the most economical, while the PV/WT/battery charging station in Zhengzhou is the least economical. The hybrid PV/WT/battery EV charging stations in the five regions can meet current requirements. The sensitivity analysis also shows that the greater the load or the number of EVs, the lower the reliability of the charging station. With a certain capacity shortage, the right number of EVs can make the charging station more economical and reliable.