Development Of Degenerative Disease Research Articles

Ageing without senescence: A critical absence in social gerontology?

This paper addresses the absence of the term ‘senescence’ in recent social science literature on ageing. The significance of this omission is considered in light of the emerging standpoint of gero-science, which argues that the central processes defining ageing are concerned with the rising probability of functional decline, development of degenerative disease and death. From this perspective, the separation of ageing and senescence sustains the myth that there exist forms of ageing that are exempt from senescence. The persistence of this myth underlies ageing studies, the sociology of later life and most social gerontology. While there have been undoubted benefits arising from this bracketing out of senescence, the argument of this paper is that the continuing advances associated with this standpoint are outweighed by the need to seriously engage with the consequences of contemporary societal ageing and the centrality of the processes of senescence in establishing an adequate understanding of ageing, its correlates and contingencies and its personal and social consequences.

Degenerative Disc Diseases: A Brief Review

Back pain of different etiology is a common encountered symptoms in daily practice. Several factors were associated with the development of degenerative disease (DDD) of the intervertebral disc (IVD).
 The intervertebral disc, usually asymptomatic but once became symptomatic it will be presented as a low back pain with is consider the main complain among the patient with IVD. Both genetic as well as environmental factors lead to progressive redaction in extracellular matrix (ECM) composition which resulted overtime in weak IVD. Clinically back pain divided into two main categories: Inflammatory versus mechanical back pain, DDD typically present with mechanical type lower back pain.
 In patient with compatible history and physical exam findings, diagnosis of DDD can be confirmed by computed tomography (CT) scan, magnetic resonance imaging (MRI), or provocative discography.
 The DDD can be manage with several treatment strategies such as conservative therapy which aim to relief the pain, on the other hand, surgical approach which aim to definitely treat the DDD. The current study aims to provide a brief review about degenerative disc diseases.

Total phenolic content and antioxidant activity of limestone endemic Araceae species, Alocasia farisii

The changing environments are giving a rise to free radical, causing development of degenerative disease. A search for natural antioxidant is required as the synthetic antioxidant reported has carcinogenic effects on living organisms. Therefore, the aim of this study is to determine the total phenolic content and antioxidant activity of Alocasia farisii leaves and petioles using three different polarity solvent which are methanol, ethanol and ethyl acetate. The total phenolic content was evaluated using the Folin-Cioucalteu reagent with some modification and the antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay. The methanolic extract attained the highest total phenolic content and antioxidant activity at 46.615 μg GAE/g and 66.43 %, respectively. Ethyl acetate with the lowest polarity had the lowest value, 34.769 μg GAE/g total phenolic content and 58.274 % in antioxidant activity. The IC50 value shows methanol recorded the lowest value at 339.905 μg/mL, indicates high radical scavenging activity whereas ethyl acetate has highest IC50 value (400 μg/mL) indicates low radical scavenging. These finding provide useful information on the total phenolic content and antioxidant activity of A. farisii that can be a reference for further research on this species of Araceae family. The leaves and petiole extracts of A. farisii may be exploited as natural sources of antioxidant.

The role of infectious pathogens in etiopathogenesis of degenerative intervertebral disc disease

Objective. To analyze the presence of infectious pathogens in the intervertebral disc tissues as possible pathogenetic factor in the development of degenerative diseases of the spine. Material and Method. In 2017–2018, a prospective exploratory research was conducted, which included 64 patients with degenerative disease of the spine who met the following inclusion criteria: the presence of clinically and instrumentally proven hernias of the intervertebral discs in the lumbosacral spine, the absence of a history of surgical interventions on the spine, non-intake of antibacterial drugs for the year before surgery, and Modic I and /or II type changes on MRI. All patients underwent interventions with sampling of intervertebral disc material; a total of 80 discs were examined. The obtained materials were subjected to microbiological and histopathological studies.Results. Out of 64 patients, only 1 (1.6 %) revealed the presence of bacterial flora in the intervertebral disc tissue (Propionibacterium acnes strain). It can be assumed that the identified bacterial flora is the result of contamination. It is also possible that the infectious agent joined already during the development of degenerative disease of the spine. If the bacterial flora was a pathogenetic factor, then it can be assumed that the pathogenesis of the disease is polyetiological and includes the infectious process as a rare event.Conclusions. Given the high interest of specialists in the pathogenesis of degenerative diseases, it seems appropriate to conduct further studies in this direction using various laboratory diagnostic methods with high sensitivity and specificity.

TOTAL ARTHROPLASTY AND ANTERIOR CERVICAL DISCECTOMY WITH FIXATION: LONG-TERM RESULTS OF A RANDOMIZED CLINICAL TRIAL

Objective.To perform comparative analysis of the long-term results of using the methods of total cervical disc arthroplasty (CDA) and anterior cervical discectomy and fusion (ACDF) in the surgical treatment of patients with single-level degenerative diseases of cervical intervertebral discs.Material and Methods.The study included 186 patients aged 21–60 years. Independent sequential randomization (1:1) of 173 patients was performed using software. The following parameters were used to evaluate patients: the VAS score of pain syndrome severity in the cervical spine and upper extremities, the Neck Disability Index (NDI) score of the quality of life, the amplitude of movements of the operated segment, the frequency of adjacent discs degeneration and of repeated surgical interventions and adverse events.Results.In the long-term follow-up, the best clinical outcomes according to VAS and NDI data were recorded in patients from the CDA group. Their amplitude of movements in the segment remained in the physiological volume. In the ACDF group, a complete fusion was verified in 83 (93.3 %) cases. A significantly higher degree of degenerative disease of superjacent intervertebral discs was revealed in ACDF group (p < 0.01), while no significant degenerative changes were recorded in the subjacent discs (p > 0.05). The number of intraoperative and early postoperative complications did not have a statistically significant intergroup difference (p > 0.05). Symptomatic degeneration of adjacent segments was verified in 2 (2.4 %) respondents from the CDA group and in 8 (9.0 %) from the ACDF group (p < 0.001). Symptomatic adverse effects were found in 3 (3.6 %) CDA patients in the form of heterotopic ossification and in 6 (6.7 %) ACDF patients in the form of pseudoarthrosis.Conclusions.The operations of total disc arthroplasty (CDA) and anterior cervical discectomy and fusion (ACDF) are safe and effective methods of surgical treatment of patients with single-level degenerative diseases of cervical intervertebral discs. In CDA patients, significantly better clinical results were noted, as compared with the ACDF group. The CDA method allowed preserving the normal biomechanics of the cervical spine and preventing the development of degenerative disease of adjacent segments.

Biology and mechano-response of tendon cells: Progress overview and perspectives.

In this review, we summarize the group discussions on Cell Biology & Mechanics from the 2014 ORS/ISMMS New Frontiers in Tendon Research Conference. The major discussion topics included: (1) the biology of tendon stem/progenitor cells (TSPCs) and the potential of stem cell-based tendon therapy using TSPCs and other types of stem cells, namely, embryonic and/or induced pluripotent stem cells (iPSCs), (2) the biological concept and potential impact of cellular senescence on tendon aging, tendon injury repair and the development of degenerative disease, and (3) the effects of tendon cells' mechano-response on tendon cell fate and metabolism. For each topic, a brief overview is presented which summarizes the major points discussed by the group participants. The focus of the discussions ranged from current research progress, challenges and opportunities, to future directions on these topics. In the preparation of this manuscript, authors consulted relevant references as a part of their efforts to present an accurate view on the topics discussed.

Does Anterior Cruciate Ligament Reconstruction Lead to Degenerative Disease?

Background: Reporting of long-term outcome of anterior cruciate ligament (ACL) reconstruction with the patellar tendon (bone–patellar tendon–bone [BTB]) autograft is limited. There are concerns that degenerative joint disease is common in the long term, which may be associated with the procedure itself. Hypotheses: (1) ACL reconstruction with BTB provides good long-term outcome. (2) There are additional factors to surgical reconstruction that can be associated with the development of degenerative disease. Study Design: Case series; Level of evidence, 4. Methods: Of 161 patients, 114 were eligible. Patient-centered outcome was by Lysholm and subjective International Knee Documentation Committee (IKDC) score; objective outcome measures were clinical examination and IKDC radiological grade. Results: Mean average follow-up was 13 years. The IKDC radiological grades in the worst compartment were A = 15%, B = 51%, C = 19%, and D = 14% (n = 83). There was a significant difference between the injured versus contralateral uninjured knee (n = 42, P = .003). In a subgroup with no meniscal or chondral injury the IKDC grades were A = 38%, B = 55%, C = 7%, and D = 0% (n = 29). The mean subjective scores were 89 ± 11 (Lysholm) and 83 ± 15 (IKDC) (n = 114). Poor IKDC subjective outcome was associated with chondral injury (P = .001), previous surgery (P = .022), return to sport (P = .013), and poor radiological grade in the ipsilateral medial compartment (P = .004). A poor IKDC radiological grade was associated with chondral injury (P = .002), meniscal injury (P = .010) and meniscectomy (P = .012), an IKDC subjective score of <85 (P = .01), and poor radiological grade in the contralateral medial compartment (P = .041). Conclusion: At 13 years, BTB ACL reconstruction provides a good outcome. Chondral and meniscal damage at surgery were associated with a poor radiological outcome, indicating that injuries sustained during ACL rupture may be the main predictors of degenerative bone disease.

PC43 LONGTERM EFFECTS OF PRE- AND POSTNATAL EXPOSURE TO LOW AND HIGH DIETARY PROTEIN LEVELS: EVIDENCE FROM EPIDEMIOLOGICAL STUDIES AND CONTROLLED ANIMAL EXPERIMENTS

Introduction: Epidemiological evidence relates low birth weight to increased risk for syndrome X, coronary heart disease, and high blood pressure in adult age. Methods: This paper reviews evidence on effects of low and high dietary protein in the development of degenerative disease. Results: Low birth weight was linked to maternal and fetal malnutrition and subsequent fetal growth retardation but there are few epidemiological studies analysing the effect in humans. Their outcome is inconclusive in establishing a relationship between dietary protein in pregnancy and birth weight (Metges 2001). A hypothesis was put forward that surviving poor nutrition in utero programs a ‘thrifty phenotype’ offspring prone to poor health in adult life when exposed postnatally to adequate or excess dietary supply (Hales & Barker 1992). An established model to study fetal programming by maternal malnutrition and exploring mechanisms responsible for the development of adult degenerative disease is the maternal low protein (MLP) model in rodents. Usually a 40–50 % protein restricted casein-based diet is compared with an adequate diet of 18–20% protein during pregnancy. In various studies evidence for the above hypothesis was generated. MLP diet throughout pregnancy results in offspring with age-related loss of glucose tolerance, insulin resistance, hypertension, and possibly obesity (e.g. Ozanne & Hales 1999). However, not only MLP diet results in low birth weight in rats, also maternal protein overnutrition was followed by a reduction of body weight at day of life 2 but a higher weight in wk 6 in the offspring (Daenzer et al. 2002). Exposure to high protein diets during pregnancy and lactation resulted in a decreased body weight of pups until weaning. The offspring of maternal high protein feeding had a higher body fatness and a reduced total energy expenditure at wk 9. In contrast, postnatal protein overnutrition only did not lead to an obese phenotype. So far there is no single mechanism identified explaining how maternal factors change the intrauterine environment to increase disease risk in adult offspring. However, it was suggested that due to inadequate maternal nutrient supply the maternal-fetal nutrient (e.g. glucose, amino acids) and hormonal transfer alters the intrauterine environment with parallel changes of the hormonal setpoints in the fetus. This may lead to persistent modifications in the offspring lasting the whole life. In particular the hypothalamo-pituitary-adrenal axis is thought to play a role in fetal programming. Conclusion: There are indications that also other hormonal systems may be involved which needs further study.

Effect of Chronic Exposure to Ultraviolet Radiation and Photoreactivation on Life Span and Tumor Development in the Marsupial Monodelphis domestica

The effect of exposure to chronic ultraviolet (UV) radiation on life span was examined in Monodelphis domestica, which is capable of photoreactivation repair of UV-radiation-induced pyrimidine dimers. Shaved Monodelphis were exposed to 500 J/m2 UV radiation, 500 J/m2 UV radiation then 90 min of photoreactivating light (PRL), or 90 min of PRL three times weekly for 104 weeks. Opossums were weighed weekly; samples for serum chemistry and hematology testing were obtained periodically. Complete postmortem examinations revealed a primary cause of death for each opossum. Meaningful differences among the groups in weight gain, serum chemistry values or hematology values were not seen. Significant life-shortening due to UV-radiation exposure was found for females but not males. Photoreactivation prolonged life only in the females exposed to UV radiation. Exposure to UV radiation was not associated with accelerated development of degenerative disease. Significant treatment-related mortality occurred in both male and female opossums exposed to UV radiation. Photoreactivation reduced the relative risk of skin tumors but not eye tumors in Monodelphis exposed to UV radiation. Eye and skin tumors were less likely to be a cause of death in UV-radiation-exposed opossums subsequently exposed to PRL than in opossums exposed to UV radiation alone. Females exposed only to UV radiation had an increased risk of skin tumor development relative to males.