To test the influence of growth rate on the pattern of fatty acids, Scenedesmus obliquus was exposed, in a chemostat culture, to different dilution rates, as these determine growth rates. Dilution rates varied from 0.0 to 0.48 d −1 (0.0 – 0.15 – 0.33 – 0.45 – 0.48 d −1). In total, only few fatty acids showed a significant correlation to the dilution rate. However it seemed, that algae from the chemostat with zero flow-through differed more from steady state growing algae (i.e. positive dilution rates) in their patterns of fatty acids than within positive growth rates. Also, carbon and nitrogen were measured and C:N-ratios were calculated. The C:N-ratios support the grouping of the samples into two categories: “zero dilution rate” and “positive growth rates at steady state”.
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