Determination Of Cholic Acid Research Articles

Determination of cholic acid in body fluids by β‑cyclodextrin-modified N-doped carbon dot fluorescent probes.

An easy, dependable, and sensitive cholic acid activity experiment was designed based on β‑cyclodextrin-modified carbon dot (β‑CD-CD) nanoprobes with specific host-guest recognizing ability and photoelectron transfer capability. The β‑CD-CD nanoprobes were characterized by infrared, ultraviolet-visible, and fluorescence spectroscopy and transmission electron microscopy. The fluorescence of the probes under optimized conditions linearly responded to cholic acid concentration from 0 to 650 μmol·L-1 with a detection limit of 25 nmol·L-1. The probes also performed well in detecting cholic acid in serum and urine samples with an average recovery rate of 97.1%-103.4%. Thus, this study provides a reliable, rapid, and easy method of cholic acid detection in body fluids that can be potentially applied in medical studies.

Polymeric membrane electrodes for drug analysis

Two new polymeric membrane electrodes selective to cholate and to benzylpenicillinate, based respectively on PVC membranes containing polybenzyl-propargilamine as conductor polymer and benzyldimethylcethylammonium-cholate, or -benzylpenicillinate as exchangers, have been prepared, and applied to the determination of cholic acids, anionic surfactants and antibiotics. Results are compared with those ones obtained by analogous PVC sensors and liquid membrane sensors, previously described.

Differential-pulse polarographic determination of cholic acids.

A differential-pulse polarographic technique is described for the determination of the concentration of cholic acids in pharmaceutical products and in human bile. The procedure is carried out at a temperature of 25 °C, an ionic strength of 0.4 mol l–1 and a pH of 5.25. The similar polarographic behaviour of the peak height versus the concentration of the unconjugated and conjugated acids allows the evaluation of the total cholic acids content by summing the independent forms in which they are present in the sample. The method is particularly good for the determination of cholic acids in human bile because it is cheap, very fast and sufficiently acurate and precise results are obtained without pre-treatment of the sample.The results are comparable to those obtained by the enzymatic-spectrophotometric method. The linearity range in the solution to be polarographed lies between 2 × 10–6 and 1 × 10–4 mol l–1, depending on the nature of the acid; the relative standard deviation of a single measurement on five different samples is less than 1% for pure cholic acid solutions, between 3.3 and 9.2% for pharmaceuticals and 2.4 and 8.9% for human bile.

Determination of Cholic Acids by Ion Selective Liquid Membrane Electrode in Pharmaceutical Products

Abstract An ion selective electrode based on a liquid membrane, constituted by a solution of tributylcetylphosphonium benzoate in nitrobenzene, is employed to determine cholic acids in pharmaceutical products by calibration curve and standard addition methods. The results are compared with those obtained with enzymatic and calorimetric methods. The proposed technique is simple, rapid and sufficently accurate.

魚体脂質の代謝に関する研究-II

Mackerel liver was analysed for fat-and cholic acid-contents. Cholic acid is commonly found in bile which is indespensable in digestion, absorption, and transportation of fat. Determination of cholic acid was made by the colorimetry standing on the INOUE's reaction. Although no correlation was found between the contents of fat and cholic acid, there was a tendency that the cholic acid level is higher in smaller liver than in bigger one: the former (<6.5g.) contained cholic acid above 100mg.% and the latter (>6.5g.) below 100mg.%. Total content of cholic acid in one liver is seemed to be lie in the limited range between ca. 2.5 and 8.2mg., in most cases 2.5-5.0mg. Then it is presumable that the cholic acid content in liver is controlled in constant level by some physiological function.

Determination of Cholic Acid in Bile and in Duodenal Drainage

The Gregory-Pascoe1 reaction was found to be specific for the determination of cholic acid. The Reinhold and Wilson2 modification of this method was combined with the procedure of Harwood3 in such a fashion as to obviate both the use of the color filter and the necessity of carrying out the reaction in the presence of alcohol. In addition it was found that the large amount of deoxycholic acid present in human bile, although not taking part in the reaction, formed a precipitate which interfered markedly with the colorimetric procedure. This difficulty was overcome by the addition of alcohol after the Gregory-Pascoe reaction had been carried out to completion.Method. 1-2 cc. of human or canine gall bladder bile, 3-5 cc. of canine fistula bile, 10-30 cc. of human fistula bile or 20-35 cc. of duodenal drainage material are the usual quantities measured out into a 50 cc. centrifuge tube. Three cc. of 2N KOH is then added and thoroughly mixed with the bile. When small quantities of concentrated bile are used, 1...

The determination of cholic acids in blood

The determination of cholic acids in blood