Determination Of Catecholamines In Urine Research Articles

Rapid semiautomated method for the differential determination of urinary catecholamines.

A semiautomated method is presented for the determination of adrenaline and noradrenaline by a combination of the trihydroxyindole procedure for total catecholamines and a new procedure for the specific direct determination of adrenaline without interference from noradrenaline at physiological levels. This new procedure involves the reaction of adrenaline with alkaline thiosemicarbazide to produce a fluorophor of acceptable stability for use in automated procedures. Its structure is as yet unknown. The within-batch standard deviation for the trihydroxyindole and alkaline thiosemicarbazide procedures respectively were 0.005 mumol/24h at a concentration of 0.368 mumol/24h and 0.012 mumol/24h at a concentration of 0.133 mumol/24h. The between-batch standard deviations of the two procedures were respectively 0.018 mumol/24h at a concentration of 0.388 mumol/24h and 0.015 mumol/24h at a concentration of 0.111 mumol/24h. Normal ranges for urine are presented for 24 healthy ambulant laboratory staff. The total catecholamine concentration (calculated as adrenaline) was 0.452 +/- 0.069 mumol/24/ (SD), range 0.349-0.616 mumol/24h. Using the alkaline thiosemicarbazide procedure, the normal range which was determined for adrenaline was 0.100 +/- 0.054 mumol/24h, range 0.022-0.207 mumol/24h. Noradrenaline calculated by difference was 0.355 +/- 0.081 mumol/24h, range 0.195-0.497 mumol/24h.

Left ventricular function and adrenergic hyperactivity before and after saphenous vein bypass.

Forty patients with severe angina pectoris were studied before and two weeks after saphenous vein bypass surgery (SVG) in order to assess the effect of this operation on left ventricular performance as judged by systolic time intervals (STI). The patients were divided into two groups: group I included 29 patients in whom no postoperative infarction occurred and group II was composed of 11 patients with postoperative infarction. For group I the PEP/LVET was 0.39 +/- 0.01 preop and slightly but significantly increased at 0.42 +/- 0.004 (P less than 0.025) two weeks postop. The mean preop PEP/LVET was 0.33 +/- 0.01 for group II and dramatically increased to 0.54 +/- 0.02 (P less than 0.001) after surgery. Another striking abnormally was a marked shortening of electromechanical systole (QS2I), which was uniformly present in the postoperative studies. Follow-up studies in 16 patients and urinary catecholamine determination in five patients suggested excessive adrenergic activity was responsible for the abbreviated QS2I. This phenomenon must be considered when interpreting the results of SVG on left ventricular function.

Norepinephrine and epinephrine secreting paraganglioma of the jugular glomus

A case of glomus jugulare tumor secreting norepinephrine and epinephrine is reported. Further support is given to the neuroectodermal origin and to the embryological similarity to the adrenal medulla. The importance of urinary catecholamine determination in all patients suspected of having a paraganglioma is stressed. Venous sampling for plasma catecholamine determination at the time of angiography may help to localize the tumor and to avoid exploratory laparotomy.

Détermination différentielle des catécholamines urinaires

A method for the differential determination of urinary catecholamines is described, using the formation of hydroxy-indolic derivatives by oxidation; it was developed for routine determinations, giving good accuracy, specificity and its simplicity is adequate. Each step was studied, particularly to test the limits of the method. The main points are: saturation of the reactions, mutual interference of catecholamines, interference of several compounds, like Dopa, α-methyl Dopa, etc. In fact, except in cases of highly abnormal urines (for example, in neural crest tumors or during treatment with α-methyl Dopa), saturations and interferences can be neglected.

Interference by Formaldehyde-forming Drugs in the Determination of Urinary Catecholamines

Journal Article Interference by Formaldehyde-forming Drugs in the Determination of Urinary Catecholamines Get access M O Klotz, M O Klotz Department of Biochemistry, Ottawa Civic Hospital, Ottawa 3, Canada Search for other works by this author on: Oxford Academic Google Scholar Helmut Richter, Helmut Richter Department of Biochemistry, Ottawa Civic Hospital, Ottawa 3, Canada Search for other works by this author on: Oxford Academic Google Scholar Mary Meuffels Mary Meuffels Department of Biochemistry, Ottawa Civic Hospital, Ottawa 3, Canada Search for other works by this author on: Oxford Academic Google Scholar Clinical Chemistry, Volume 10, Issue 4, 1 April 1964, Pages 372–373, https://doi.org/10.1093/clinchem/10.4.372 Published: 01 April 1964 Article history Received: 01 March 1963 Published: 01 April 1964

HUMAN URINARY CATECHOLAMINES IN RELATION TO CLIMATE.

Urinary catecholamine determinations were employed to appraise human sympathoadrenal activity in different seasons (autumn, winter, and spring), the study being made in a subtropical climate, where maximum temperature in these seasons averaged, respectively, 66, 60, and 74 F. Two hundred and thirty-one healthy men were studied under standardized laboratory conditions, and significant variation with season (P < .01) was established for excretion of both epinephrine and norepinephrine and for the norepinephrine-to-epinephrine ratio, the latter index providing the most clear-cut pattern of variation with season, and suggesting greater activity in the sympathoadrenal system in winter than in autumn, with reversal in spring. Cigarette smoking and anxiety, acting singly or jointly, had modifying effects on climate-induced changes in epinephrine excretion. chronic cold effects in man; bioclimatology; seasonal sympathoadrenal activity; epinephrine and norepinephrine excretion in chronic cold; smoking and epinephrine excretion; anxiety and epinephrine excretion Submitted on May 13, 1963

Chemical determination of urinary catecholamines; an aid in the differential diagnosis of essential hypertension and pheochromocytoma.

The classical description of patients with pheochromocytoma includes, as typical symptoms, paroxysmal hypertension, pallor, sweating, and tachycardia. It is now known that only a minority of patients exhibit such symptoms. 1,2 The majority of cases manifest only a persistent hypertension and are difficult to differentiate from cases of essential hypertension. The proportion of pheochromocytomas among cases of hypertension, while small, is significant. Graham 3 encountered 8 cases, or 0.47%, of pheochromocytoma among 1700 unselected patients with hypertension subjected to bilateral lumbodorsal splanchnicectomy. If this percentage is correct, approximately 1 out of every 200 cases of hypertension involves a pheochromocytoma. It thus becomes important to have a highly specific method for the diagnosis of the latter disease. The pharmacologic tests, such as the histamine, benzodioxane, and phentolamine (Regitine) tests, are known to yield both false-negative and false-positive tests. 1,4,5 Since Engel and von Euler 6 first pointed out in 1950 the