Determination Of Antioxidant Capacity Research Articles

Determination of total phenolic content and antioxidant capacity of blueberries using Fourier transformed infrared (FT-IR) spectroscopy and Raman spectroscopy

This study investigated the feasibility of using Fourier-transform infrared (FT-IR) and Raman spectroscopies to quantify total phenolic content and antioxidant capacity of blueberries. Blueberry extracts were applied to determine total phenolic content (Folin–Ciocalteu assay) and antioxidant capacity [oxygen radical absorbance capacity (ORAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)], while pressed juices were used for spectroscopic analysis. Six partial least squares regression models with cross-validation were developed using FT-IR and Raman spectra of blueberries from 11 locations and their corresponding chemical testing values, followed by prediction tests using samples from another five locations. FT-IR prediction models show relatively good prediction power for ORAC, DPPH, and Folin–Ciocalteu values (R2-prediction = 0.6359, 0.6580, and 0.8092; RMSE-prediction = 6.19 and 0.71 µmol trolox equivalents/g fresh weight, and 0.14 µg GAE/g fresh weight), but Raman prediction models did not yield a satisfactory result. FT-IR spectroscopy may be used to rapidly determine blueberry antioxidants.

A Graphene Quantum Dots-Hypochlorite Hybrid System for the Quantitative Fluorescent Determination of Total Antioxidant Capacity.

Antioxidants play a major part in the prevention and impairment of oxidative stress-induced damages and diseases. Evaluating the antioxidants activity/capacity in food and biological fluid is proved to be useful for the diagnosis and treatment of these oxidative stress-induced diseases. Herein, a graphene quantum dots (GQDs)-hypochlorite system to detect the antioxidants including nonenzymatic and enzymatic antioxidants in the biological fluid is proposed. The detection principle is based on the fact that antioxidants can protect the fluorescence of GQDs from hypochlorite-caused quenching by acting as the scavengers of hypochlorite. The GQDs-hypochlorite system allows the accurate quantification of the total antioxidant capacity (TAC) of commercial drinks as well as the extracellular superoxide dismutase (SOD) secretion upon stimulation of cytokines or hyperglycemia. This system shows the excellent analytical recoveries for commercial drinks (>89.9%) and good consistency with ELISA testing for SOD secretion in cell-conditioned medium. These results demonstrate the ability and reliability of the GQD-hypochlorite system for detecting and quantifying TAC in real drinks and complex biological fluids.

ANTIOXIDANT CAPACITY AND PHENOLIC CONTENT OF FRAXINUS ORNUS L. AND FRAXINUS PENNSYLVANICA MARSCH. LEAVES AND BARK EXTRACTS

UDK: 582.916.16:547.56
 547.56:543.42
 Total contents of phenols, flavonoids, phenolic acids and proanthocyanidins have been determined in methanolic extracts of Fraxinus ornus L. and Fraxinus pennsylvanica Marsch. leaves and branches bark. DPPH and FRAP assay were used in the determination of antioxidant capacity. F. ornus leaves had higher contents of flavonoids (13.08 mg RE g-1 DW) and proanthocyanidins (7.29 mg LCE g-1 DW) while the bark had higher contents of phenols (56.47 mg GAE g-1 DW), phenolic acids (14.32 mg CAE g-1 DW) and coumarins (94.81 mg CE g-1 DW). F. pennsylvanica leaves were richer in contents of phenols (25.73 mg GQE g-1 DW), flavonoids (2.87 mg CE g-1 DW and 5.13 mg RE g-1 DW), phenolic acids (14.60 mg CAE g-1 DW) and coumarins (20.01 mg CE g-1 DW) while the bark had more proanthocyanidins (6.88 mg CE g-1 DW). F. ornus leaves had lower contents of phenolic acids (11.09 mg CAE g-1 DW) than F. pennsylvanica leaves. Also, F. pennsylvanica bark had higher contents of flavonoids (1.25 mg RE g-1 DW) and proanthocyanidins (6.88 mg CE g-1 DW) than F. ornus bark. Antioxidant capacity for both species was higher for the leaves than the bark. Generally, F. ornus had better antioxidant capacity than F. pennsylvanica. Very high correlations were found between antioxidant capacity and phenols (r2= 0.9361-0.9805), flavonoids (r2= 0.9358-0.9876) and coumarins (r2= 0.9661-0.9938) in leaves. In bark, correlations were found for phenols (r2= 0.9744-0.9796) and coumarins (r2= 0.9757-0.9911).

Anthelmintic activity of Tunisian chamomile (Matricaria recutita L.) against Haemonchus contortus

The chemical treatment of gastrointestinal parasitic diseases has been undermined by increasing resistance and high toxicity. There is an urgent need to search for alternative natural sources for the treatment of such parasites. In this respect, the present study aims to quantify phenolic compounds of chamomile (Matricaria recutita L.) and to study their in vitro anti-oxidant and anthelmintic activities in solvents with increasing polarity. In vitro determination of anti-oxidant capacity was carried out using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation methods. In vitro anthelmintic activity was investigated on egg-hatching inhibition and loss of motility of adult worms of Haemonchus contortus from sheep. The results showed that methanolic and aqueous extracts contain more total polyphenols, total flavonoids and condensed tannins than chloroformic and hexanic extracts. ABTS and DPPH assays showed that methanolic extracts had the highest anti-oxidant potency (IC50 = 1.19 μg/ml and 1.18 μg/ml, respectively). In vitro anthelmintic activity showed that both methanolic (IC50 = 1.559 mg/ml) and aqueous (IC50 = 2.559 mg/ml) extracts had the greatest effect on egg hatching and motility of worms (100% after 8 h post exposure at 8 mg/ml). A significant and positive correlation between DPPH and ABTS tests was observed for all tested extracts. Therefore, total phenolic, total flavonoid and condensed tannin values were correlated with IC50 from both ABTS and DPPH, and with inhibition of egg hatching. To our knowledge, this report is the first of its kind to deal with in vitro anthelmintic activities of chamomile extracts.

Estudio preliminar del contenido polifenólico y capacidad antioxidante de la especie Malus domestica cultivada en Costa Rica

<p class="p1">Especies como la manzana de altura han sido sometidas en Costa Rica a un proceso de domesticación para adaptarlas a las condiciones climáticas de ese país y específicamente a las de la Zona de los Santos de Costa Rica. Este esfuerzo ha llevado a la obtención en la actualidad de productos que son bien aceptados en el mercado por su sabor, olor, facilidad de manipulación, entre otros. Con el avance del conocimiento biológico y químico, los estudios del contenido de polifenoles en este tipo de frutos se han incrementado, demostrando que estos poseen propiedades bioactivas, tal como su potencial antioxidante, siendo este conocimiento un factor importante en la creciente utilización de frutas y suplementos nutricionales con contenido polifenólico importante a nivel internacional y en la dieta de los costarricenses. De allí, que el presente trabajo se enfoca en realizar estudios en frutos de altura cultivados en Costa Rica, tanto desde el punto de vista de mejora agroecológica, así como de la determinación del contenido de polifenoles y su capacidad antioxidante. Se adquirieron frutos de dos variedades de la especie <em>Malus domestica</em>, a saber, manzana variedad Ana y Jonas, que fueron procesados en el laboratorio con el fin de ser utilizados para estas determinaciones. Además, se trabajó en la mejora de las condiciones agroecológicas para el cultivo con el uso de microorganimos de biocontrol contribuyendo a la protección de suelo. Esta publicación presenta información preliminar de estos estudios, dada su relevancia para el conocimiento científico con resultados que se publican por primera vez para esta especie económicamente importante cultivada en Costa Rica.</p>

Determination of polyphenol component fractions and integral antioxidant capacity of Syrian aniseed and fennel seed extracts using GC–MS, HPLC analysis, and photochemiluminescence assay

The chemical components of both Syrian aniseed and fennel seeds collected from Idlib and Aleppo regions were identified using GC/MS spectrometry. Thirty-four component fractions representing an average of 98.5% of anise seed extract were characterized. The major average components were (E)-Anethole, Estragole, Limonene, Fenchone, Linalool, para-Anisaldehyde, (Z)-Anethole, and methyl chavicol. Also, thirty-one component fractions representing an average of 99.46% of fennel seed extract were identified. The major components were α-Pinene, α-Phellandrene, Fenchone, Methyl chavicol, Estragole, (Z)-Anethole, Limonene, 1,8-Cineole, Anisole-p-allyl, E,E-Farnesene, Estragole, (E)-Anethole, Anisaldehyde, and Carvacrol. The extract of aniseed was characterized by higher amounts of trans-anethole (52.2%) than fennel extract (38.3%). The integral antioxidant capacity (IAC) of two different extracts from both aniseed and fennel seeds have been determined using photochemiluminescence assay (PCL). The sum of antioxidant capacity (IAC) of hydrophilic and hydrophobic components in both aniseed and fennel seeds was determined, as a total equivalent per gram of dry material (nmolTE/gDM) at 166.69 ± 0.42 and 363.28 ± 1.11 nmol for hydrophilic and hydrophobic components, respectively. Eight major phenolic compounds known as possible antioxidant sources with their concentrations have been identified in both aniseed and fennel seeds by HPLC and GC–MS analysis.

Physicochemical Characterization, Standardization and In vitro Determination of Radical Scavenging Activity of Zereshk-e- Saghir, A Traditional Preparation, and Its Ingredients

: Objective: Quality control and standardization of traditional preparations ensure their safe, pure and efficient prescription. Zereshk-e-Saghir (ZES), a folk remedy, has been recommended as a hepatoprotective agent in Traditional Persian Medicine (TPM). This study aimed at the evaluation of the physicochemical and phytochemical characterization, standardization, and in vitro antioxidant capacity determination of ZES and its ingredients. Method: Some qualitative and quantitative controls were performed like ash value, moisture content, extractable matter, phytochemical screening, TLC fingerprint and microbial contamination. Total phenolic and emodin contents of ZES were measured by spectrophotometric method to standardize this preparation. Finally in vitro radical scavenging activity of ZES and its ingredients were determined using 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) assay. Result: Regarding the physicochemical evaluation, the amount of each parameter (ash value, moisture content, and extractable value) was determined as documented characteristics for ZES components. Phytochemical screening of ZES ingredients indicated the presence of tannins in all the samples and the presence of alkaloids, steroids and terpenoids in a majority of the samples. Meanwhile, ZES exhibited no pathogenic contamination. The emodin and total phenolic contents (TPC) of ZES were reported 457.0 μg emodin/100g and 381.5 mg gallic acid equivalents (GAE)/100 g of ZES, respectively. Also TPC ranged from 3607.4 to 27.9 mg GAE/ 100 g of ZES ingredients. Meanwhile, the results demonstrated that ZES could scavenge DPPH radicals (IC50value: 266.4 μg/ ml). Conclusion: The obtained results on quality control and standardization of ZES could be proposed as a monograph having standard characteristics.Key words: DPPH Assay, Physichochemical Characterization, Quality Control, Standardization, Traditional Medicine.

Properties of Antioxidant and Anti-Inflammatory Activity and Phenolic Profiles of Şevketi Bostan (Cnicus benedictus L.) Cultivated in Aegean Region from Turkey

The aim of this work was to evaluate the phenolic profiles and anti-inflammatory, antioxidant and gastro-protective activities of Cnicus benedictus L., a type of milk thistle cultivated in Turkey. The total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP) and the 2,2-diphenyl-1-picryhydrazyl (DPPH) free radical scavenging activity was measured to determine antioxidant capacity. The anti-urease and anti-xanthine oxidase activities were used to determine the gastro-protective and anti-inflammatory potential of the plant extracts, respectively. The TPC was 337.40 and 635.10 mg of gallic acid equivalents (GAE)/100 g and TFC was 41.05 -119.12 mg of quercetin equivalents (QE)/100 g in the root and leaf extracts, respectively. The root and leaf extracts of Cnicus benedictus L., were exhibited different inhibition values against both of the enzymes. The inhibition effect of the both enzymes were calculated as IC50 (mg/mL) in terms of 50% inhibition of the enzymes. The xanthine oxidase activity of the leaf and root was 18.53 and 19.75 mg/mL and the urease activity were 2.29 mg/mL and 11.53 mg/mL, respectively. Fifteen phenolic compounds were determined by high-performance liquid chromatography (HPLC-UV). Vanillic, silibinin B, ferulic acid and rutin were found major amount of the plant. In conclusion, the plant have high potential beneficial nutraceuticals and consumption of the together with its roots and leafs will be beneficial in terms of nutrition.

Simple and Sensitive Method of Fluorometry for Determination of Total Antioxidant Capacity

Simple and Sensitive Method of Fluorometry for Determination of Total Antioxidant Capacity

Phytochemical Screening and In Vitro Antioxidant Activity of Ethanolic Extract of Cassia occidentalis

The ethanolic extract of the leaves of Cassia occidentalis (Co) were subjected to phytochemical analysis by standard qualitative analysis and the invitro antioxidant activity was evaluated by determination of total antioxidant capacity, 1.1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, hydrogen peroxide (H2O2) radical scavenging activity, superoxide scavenging activity and Ferric reducing anti oxidant potential (FRAP). The analyses revealed that the ethanolic extract of Co was able to efficiently scavenge the free radicals in a dose dependant manner. The results were compared with the standard antioxidant ascorbic acid. The results have shown that crude ethanolic extract of the leaves of Co showed excellent antioxidant activity due to the presence of bioactive compounds namely alkaloids, betacyanin, cardiac glycosides, coumarins, flavonoids, phenols, steroids, saponins, tannins, terpenoids, anthraquinones and emodins.

Scanometry as microplate reader for high throughput method based on DPPH dry reagent for antioxidant assay

The stable chromogenic radical 1,1′-diphenyl-2-picrylhydrazyl (DPPH) solution was immobilized on the microwell plate as dry reagent to construct a simple antioxidant sensor. Then, a regular flatbed scanner was used as microplate reader to obtain analytical parameters for antioxidant assay using one-shot optical sensors as scanometry technique. Variables affecting the acquisition of the images were optimized and the analytical parameters are obtained from an area of the sensing zone inside microwell using the average luminosity of the sensing zone captured as the mean of red, green, and blue (RGB) value using ImageJ® program. By using this RGB value as sensor response, it is possible to determine antioxidant capacity in the range 1–25ppm as gallic acid equivalent (GAE) with the response time of 9min. The reproducibility of sensor was good (RSD<1%) with recovery at 93%–96%. The antioxidant sensor was applied to the plant extracts, such as sappan wood and Turmeric Rhizome. The results are good when compared to the same procedure using a UV/Vis spectrophotometer.

Determination of chemical composition, mineral content, antioxidant capacity and rumen degradability in various varieties of wasted date palm

This experiment was carried out in order to characterise six varieties of wasted date palm (WDP). In particular, chemical composition, including mineral content, phenolic compounds and antioxidant activity of varieties were determined. Dry matter (DM) disappearance in samples was determined at 0, 2, 4, 8, 12, 24, 48 h using the in situ method. The results of chemical composition showed that WDP varieties contained 3.23–6.27% Crude Protein (CP), 6.02–32.27% Neutral Detergent Fiber (NDF), 3.83–23.97% Acid Detergent Fiber (ADF), and 54.98–88.38% Non-Fibrous Carbohydrate (NFC) (DM basis). Kharak variety had the highest NDF and as result had the lowest NFC compared to other varieties (p < .05). In addition, all varieties were good sources of minerals, especially potassium and calcium. The total phenolic compounds value was in the range of 2.96 to 9.43% where Koloteh variety had the lowest value and Kharak variety had the highest value. The Kharak variety had the highest flavonoids content and antioxidant activity compare to other varieties (p < .05). The result of degradability showed that rapidly degradable fraction (a) of dry matter was high in all varieties except in Kharak. The Estameran had the highest and Kharak had the lowest potentially degradability fraction (D). Thus, according to the results of this experiment, it can be concluded that WDP can be used as part of the diet of ruminants that helps to reduce the costs.

Application of polyphenazine films doped with metal nanoparticles for the measurements of antioxidant capacity

Six glassy carbon electrodes modified with polyphenazine conducting polymers and metallic nanoparticles have been prepared by using electrochemical techniques with the aim of determining antioxidant capacity. The electrodes showed a cathodic peak corresponding to the H2O2 reduction, being adequate for the quantitative studies of this compound. These electrodes can be used to study the interaction between H2O2 and antioxidants. From this interaction it was measured the antioxidant capacity of ascorbic acid. This capacity, in trolox equivalents, was similar for all electrodes studied, and agreed with that previously reported using other contrasted techniques. Platinum nanoparticles modified electrodes are best from the viewpoint of durability but, provided the lower cost of silver salts, silver nanoparticles modified electrodes are also a good selection, though these electrodes must be prepared daily.

Determination of serum total antioxidant capacity in male smokers and non-smokers

Background: Smoking is characterized by increased free radicals and stress oxidative. It is reported that the smokers are more prone to cardiovascular problems caused by increased production of free radicals as well as decreased level of antioxidants. Aims and Objective: The aim of this study was to compare the total antioxidant capacity (TAC) between adult male smokers and non-smokers. Materials and Methods: A total of 74 males were involved in this study. Of them, 36 individuals were non-smokers and another 38 were smokers. Age of the smokers and non-smokers was ranged between 30 and 45 years (majority of them 30-40 years old). Venous blood was collected from individuals after an overnight fast. Blood samples were used for the estimation of serum TAC. Results: Of the 74 individuals in the study, 51% were smokers with a mean age of 35.7 ± 5.8 and 49% were controls with a mean age of 34.0 ± 4.4. Data showed that TAC was significantly higher in smokers than non-smokers (P < 0.05). Conclusion: Based on this study, we suggest that smoking is associated with decreased antioxidant capacity and stress oxidative. However, future studies should examine the potential role of smoking on oxidant/antioxidant capacity balance.

Determination of toral antioxidant capacity of saliva in sickle cell anemic patients - A cross-sectional study.

Sickle cell anemia is a congenital hemoglobinopathy characterized by deformed red blood cells. Oxidative stress plays an important role in the pathophysiology of sickle cell anaemia as it destroys free radicals, and thereby depleting the protective mechanisms such as antioxidants in serum. These antioxidants are essential to protect against harmful oxidation-reduction reactions preventing oxidative damage to the cells. To evaluate and compare the Total Antioxidant Capacity (TAC) of serum and saliva in sickle cell anemia patients. A total of 150 children aged 4-12 yrs were selected and divided into two equal groups: Children suffering from sickle cell anemia and healthy controls. Blood and saliva samples were collected aseptically from both groups and were subjected to phosphomolybdenum method. Absorbance was read spectrophotometrically at 695 nm. The concentration of total antioxidants was obtained by plotting absorbance of the test against the standard graph. TAC levels in serum (0.29±0.19) and saliva (0.29±0.14) of sickle cell anaemic patients was reduced when compared with serum (0.32+ 0.18) and saliva (0.33+ 0.16) of the healthy children. The correlation between levels of TAC in saliva and serum was found to be statistically significant in sickle cell anaemic patients. A significant correlation of the TAC was found in saliva and serum of the patients with SCA suggests that saliva could be used as a non invasive alternative for assessing the antioxidant status in patients with SCA.

A Non-invasive Real-Time Methodology for the Quantification of Antioxidant Properties in Coffee During the Roasting Process Based on Near-Infrared Spectroscopy

This paper describes the development of a real-time method based on near-infrared spectroscopy (NIRS) for determination of total antioxidant capacity (TAC) and total phenolic content (TPC) in coffee during the roasting process. The real-time non-invasive monitoring procedure involved pointing a diffuse reflectance probe directly at the roasting chamber through a glass window in order to monitor the roasting process. The figures of merit of the chemometric models to estimate TAC and TPC showed selectivity values higher than 12% and determination coefficients (R 2 P) above 0.90. The TAC and TPC profiles during the roasting procedure are discussed in terms of the antioxidant compounds likely to be present in green and roasted coffee. NIRS was found to be a satisfactory real-time tool for monitoring the content of antioxidant compounds in coffee during the roasting process, complementing other established procedures.

Combined microplate-ABTS and HPLC-ABTS analysis of tomato and pepper extracts reveals synergetic and antagonist effects of their lipophilic antioxidative components

The antioxidant capacity of 9 pure lipophilic compounds was examined by microplate-ABTS and HPLC-ABTS, using similar experimental conditions. Results obtained showed that HPLC-ABTS method can be used for a rapid determination of individual antioxidant capacity of compounds in standard solutions or complex mixtures. The application of both methods to real lipophilic extracts from tomato (Solanum lycopersicum L.), green and red peppers (Capsicum annuum) reveals possible interactions between antioxidants. Thus, synthetic mixtures of two compounds identified in tomato and peppers were measured using microplate-ABTS and HPLC-ABTS. Synergistic effects were observed between (β-carotene-capsanthin) (1:9) and (1:1), (α-tocopherol-capsanthin) (1:9), (lutein-lycopene) (9:1) and (capsanthin-δ-tocopherol) (9:1). On the contrary, antagonistic effects were observed for (lutein-δ-tocopherol) and (α-tocopherol-δ-tocopherol). The interactions observed with two-compound mixtures are not systematically observed in the natural lipophilic extracts from tomato, green and red peppers, probably since extracts are more complex and are susceptible to cause interferences.

Role of potassium permanganate ethylene on physicochemical properties, during storage of five different tomato cultivars

Although tomatoes should be ripened at room temperature to obtain the best flavour, the majority of consumers who preserve food at home store tomatoes in the fridge for 4-7 days and some people even keep tomatoes for up to 3 weeks in these conditions. Inthe present work the effects of storage time and potassium permanganate ethylene absorber sachets under refrigerated conditions (similar to those used by consumers who preserve tomatoes at home) on physicochemical properties, ascorbic acid content and antioxidant capacity of five tomato cultivars (Cherry, Cherry pera, Rama, Raf and Pera) were studied. After 25 days storage at 4±2 °C an increase in fruit pH was observed for the five cultivars without and with ethylene absorber sachets. The increase in pH was paralleled by a decrease in titratable acidity. The degradation of ascorbic acid during storage time with and without ethylene absorber sachets followed a first-order kinetic model. In general, the percent loss of ascorbic acid and antioxidant capacity measured with TEAC and ORAC methods was significantly lower in tomatoes stored with ethylene absorber sachets as compared to tomatoes preserved without these. In addition, a positive correlation was found between ORAC and TEAC methods for the determination of antioxidant capacity in all tomato cultivars.

Determination of antioxidant capacity of diverse fruits by electron spin resonance (ESR) and UV-vis spectrometries.

Twenty-one kinds of fruits including strawberry, mulberry, lemon, banana, etc. were measured for antioxidant capacity based on their ability to scavenge 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical. Vitamin C equivalent antioxidant capacity (VCEAC) was used to quantify antioxidant capacity of the studied fruits. The results were expressed as mg of ascorbic acid equivalent per 100g fruit. Each fruit was divided into two parts: harvest part (fresh fruit analyzed immediately), and liquid nitrogen frozen part (fruit frozen and pulverized in liquid nitrogen). Antioxidant capacities of both fresh and frozen fruits were determined, and VCEAC values were proved to have no significant difference. For the frozen fruits, the antioxidant capacities were measured by electron spin resonance spectroscopy (ESR) and UV-vis spectrometry. VCEAC values obtained with UV-vis and ESR range from 11.48 to 345.75mg/100g and 7.01 to 366.26mg/100g. Experimental results indicated that VCEAC values obtained by two methods were highly correlated.

Are antioxidant capacity and oxidative damage related to biological and autecological characteristics in aquatic insects?

&lt;p&gt;In this work, we study the oxidative state of nine taxa of aquatic insects [&lt;em&gt;Serratella ignita &lt;/em&gt;(Poda, 1761), &lt;em&gt;Ephemera danica&lt;/em&gt; Müller, 1764&lt;em&gt;, Crocothemis erythraea &lt;/em&gt;(Brullé, 1832), &lt;em&gt;Dinocras cephalotes &lt;/em&gt;(Curtis, 1827)&lt;em&gt;, Perla bipunctata &lt;/em&gt;Pictet&lt;em&gt;, &lt;/em&gt;1833,&lt;em&gt; Isoperla morenica &lt;/em&gt;Tierno de Figueroa &amp;amp; Luzón-Ortega, 2011, &lt;em&gt;Notonecta maculata &lt;/em&gt;Fabricius, 1794, &lt;em&gt;Gerris &lt;/em&gt;sp.&lt;em&gt;, &lt;/em&gt;and &lt;em&gt;Hydropsyche &lt;/em&gt;sp.] in order to determine the relationships between this state and the biological and environmental characteristics of the species studied. The studied taxa are all in the same life cycle stage (nymph/larva), and many of them have different maximum life span potentials. We assess the antioxidant capacity through the determination of the trolox-equivalent antioxidant capacity, the ferric-reducing/antioxidant power and the activity of the antioxidant enzymes catalase, DT-diaphorase, glutathione peroxidase, glutathione reductase, glutathione transferase, superoxide dismutase and glucose-6-phosphate dehydrogenase. Furthermore, to determine the oxidative damage, we examine thiobarbituric acid reactive substances, free malondialdehyde, protein-bound malondialdehyde, total hydroperoxides, and protein hydroperoxides. In summary, we can consider that having predatory feeding habits, having a long-life cycle and living in permanent streams with cold, well-oxygenated waters are related to a proper oxidative state in the insects that we studied. On the other hand, non-exclusive predator species living in temporary streams with warm and poorly oxygenated waters with a short life cycle have a worse oxidative state. Thus, the oxidative state of each species could be defined by an interaction of biological and autecological factors, for which the relative importance is difficult to assess.&lt;/p&gt;