Detection Of Acute Allograft Rejection Research Articles

Flow cytometric evaluation of cytotoxic peripheral blood lymphocytes in acute renal graft rejection.

The presence of the S6F1+ epitope on the surface of CD8+ lymphocytes is believed to be uniquely representative of cytotoxic subpopulations. A preliminary study was conducted to evaluate the CD8+ S6F1+ peripheral lymphocytes by flow cytometry in patients undergoing renal allograft biopsy for allograft dysfunction. Lymphocytes, obtained at the time of biopsy, were analyzed by flow cytometry with CD8-FITC/S6F1-RD1 as the test monoclonal antibody and MsIgG-RD1/MsIgG-FITC as internal control. A 100% increase in S6F1+ cells over internal control was considered to be positive result. The results were correlated with the histopathologic findings in 14 instances of allograft dysfunction occurring 26.5 +/- 11.6 days posttransplantation. The histopathologic diagnosis was acute cellular rejection in eight cases, acute tubular necrosis in four, and cyclosporine nephrotoxicity in two. Flow cytometric detection of an increase in S6F1+ cells yielded a sensitivity of 87.5% and a specificity of 83.3% for the diagnosis of acute rejection. It would appear that the use of a monoclonal antibody to detect increases in the number of CD8+ S6F1+ peripheral lymphocytes is a valuable test for the detection of acute allograft rejection in the initial period after transplantation.

568 - Detection of Acute Allograft Rejection by Indium-111 Labeled Platelet Scintigraphy in Renal Transplant Patients

No AccessJournal of Urology1 Jun 1987Detection of Acute Allograft Rejection by Indium-111 Labeled Platelet Scintigraphy in Renal Transplant Patients Margaret J. Bia, Gary Desir, Michael Ezekowitz, Robert Lange, Eileen Smith, Michael Kashgarian, and Martin Schiff Margaret J. BiaMargaret J. Bia More articles by this author , Gary DesirGary Desir More articles by this author , Michael EzekowitzMichael Ezekowitz More articles by this author , Robert LangeRobert Lange More articles by this author , Eileen SmithEileen Smith More articles by this author , Michael KashgarianMichael Kashgarian More articles by this author , and Martin SchiffMartin Schiff More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)75718-7AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail "Detection of Acute Allograft Rejection by Indium-111 Labeled Platelet Scintigraphy in Renal Transplant Patients." The Journal of Urology, 137(6), p. 245A © 1987 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 137Issue 6June 1987Page: 245A Advertisement Copyright & Permissions© 1987 by The American Urological Association Education and Research, Inc.MetricsAuthor Information Margaret J. Bia More articles by this author Gary Desir More articles by this author Michael Ezekowitz More articles by this author Robert Lange More articles by this author Eileen Smith More articles by this author Michael Kashgarian More articles by this author Martin Schiff More articles by this author Expand All Advertisement Loading ...